Th9 Cells in Peripheral Blood Increased in Patients with Immune-Related Pancytopenia by Qing Shao, Yangyang Wang, Zhaoyun Liu, Hui Liu, Yihao Wang, Yang Zhao, Lijuan Li, Rong Fu

“…CD4+ lymphocytes were sorted by magnetic beads, and the mRNA expression levels of Th9 cells related transcription factors PU.1 and BATF were detected by RT-PCR. …”
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Visfatin Induces Inflammation and Insulin Resistance via the NF-κB and STAT3 Signaling Pathways in Hepatocytes by Yu Jung Heo, Sung-E Choi, Ja Young Jeon, Seung Jin Han, Dae Jung Kim, Yup Kang, Kwan Woo Lee, Hae Jin Kim

“…We treated HepG2 cells with both visfatin and either AG490 (a JAK2 inhibitor) or Bay 7082 (an NF-κB inhibitor); we examined proinflammatory cytokine mRNA levels using RT-PCR and insulin signaling using western blotting. …”
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Methyl 3-Bromo-4,5-dihydroxybenzoate Attenuates Inflammatory Bowel Disease by Regulating TLR/NF-κB Pathways by Jing Huang, Lei Li, Liyan Xu, Lixin Feng, Yuxin Wang, Attila Gabor SIK, Meng Jin, Rongchun Wang, Kechun Liu, Xiaobin Li

“…The results showed that MBD could regulate the TLR/NF-κB pathways by inhibiting the mRNA expression of <i>TNF-α</i>, <i>NF-κB</i>, <i>IL-1</i>, <i>IL-1β</i>, <i>IL6</i>, <i>AP1</i>, <i>IFNγ</i>, <i>IKKβ</i>, <i>MyD88</i>, <i>STAT3</i>, <i>TRAF1</i>, <i>TRAF6</i>, <i>NLRP3</i>, <i>NOD2</i>, <i>TLR3</i> and <i>TLR4</i>, and promoting the mRNA expression of <i>IL4</i>, <i>IκBα</i> and <i>Bcl-2.…”
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Dorsal Root Ganglion Maintains Stemness of Bone Marrow Mesenchymal Stem Cells by Enhancing Autophagy through the AMPK/mTOR Pathway in a Coculture System by Shuaishuai Zhang, Junqin Li, Huijie Jiang, Yi Gao, Pengzhen Cheng, Tianqing Cao, Donglin Li, Jimeng Wang, Yue Song, Bin Liu, Hao Wu, Chunmei Wang, Liu Yang, Guoxian Pei

“…In addition, AMPK inhibitor compound C could significantly downregulate the protein expression of LC3 and the mRNA level of stemness genes in the coculture group. …”
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Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis by Leonidas Kandilogiannakis, Eirini Filidou, Ioannis Drygiannakis, Gesthimani Tarapatzi, Stylianos Didaskalou, Maria Koffa, Konstantinos Arvanitidis, Giorgos Bamias, Vassilis Valatas, Vasilis Paspaliaris, George Kolios

“…Similarly, HIOs elicited strong mRNA and protein mesenchymal (CXCL10) and epithelial (CXCL1, CCL2, CXCL8, and CCL20) chemokine responses in early but not late passages. …”
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Integrated analysis of cell cycle and p53 signaling pathways related genes in breast, colorectal, lung, and pancreatic cancers: implications for prognosis and drug sensitivity for... by Jiyauddin Khan, Priyanjana Ghosh, Urmi Bajpai, Kountay Dwivedi, Daman Saluja

“…Validation of hub genes at mRNA and protein levels was done via GEPIA2 and Human Protein Atlas. …”
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Inhibition of the Ubiquitin-Activating Enzyme UBA1 Suppresses Diet-Induced Atherosclerosis in Apolipoprotein E-Knockout Mice by Jiawei Liao, Xiaolei Yang, Qiuyue Lin, Shuang Liu, Yunpeng Xie, Yunlong Xia, Hui-Hua Li

“…Besides, PYR-41 also suppressed aortic mRNA expression of NADPH oxidase (Nox1, Nox2, and Nox4) and lesional oxidative stress levels, determined by DHE staining. …”
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Lactoferrin Induces Osteoblast Growth through IGF-1R by Jian-Ming Hou, En-Yu Chen, Fan Lin, Qing-Ming Lin, Ying Xue, Xu-Hua Lan, Man Wu

“…Results. (1) 1, 10, 100, and 1000 μg/mL lactoferrin induced the expression of IGF-1 mRNA and protein. 10 μg/mL and 100 μg/mL lactoferrin induced the expression of IGF-1R mRNA and protein. (2) Lactoferrin (100 μg/mL) induced osteoblast proliferation while inhibiting apoptosis. …”
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Identification of EGR1 as a Key Diagnostic Biomarker in Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Through Machine Learning and Immune Analysis by Wu X, Pan T, Fang Z, Hui T, Yu X, Liu C, Guo Z, Liu C

“…Additionally, functional enrichment analysis, immune infiltration analysis, and immune function analysis were conducted. A TF-miRNA-mRNA network was constructed, and single-cell RNA sequencing was used to determine the distribution of key genes within key cell clusters. …”
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IL-33 Deficiency Attenuates Lung Inflammation by Inducing Th17 Response and Impacting the Th17/Treg Balance in LPS-Induced ARDS Mice via Dendritic Cells by Li Cheng, Yang Jiao, Wei Jiang, Xin Zhang, Liping Zhang, Gongwei Jia

“…Furthermore, depletion of IL-33 inhibited Th17 cells, significantly decreased RORγt mRNA and protein expression and IL-17 levels in BALF, and led to less differentiation of T cells into Th17 cells than Treg cells. …”
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Metallothioneins 1 and 2 Modulate Inflammation and Support Remodeling in Ischemic Cardiomyopathy in Mice by Georg D. Duerr, Daniela Dewald, Eva J. Schmitz, Luise Verfuerth, Katharina Keppel, Christine Peigney, Alexander Ghanem, Armin Welz, Oliver Dewald

“…Hearts were examined with M-mode echocardiography and processed for histological and mRNA studies. Results. Expression of MT1/2 mRNA was transiently induced during repetitive I/R in WT-mice, accompanied by a transient inflammation, leading to interstitial fibrosis with left ventricular dysfunction without infarction. …”
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Effect of High, Medium, and Low Molecular Weight Hyaluronan on Inflammation and Oxidative Stress in an In Vitro Model of Human Nasal Epithelial Cells by Giusy Daniela Albano, Anna Bonanno, Luca Cavalieri, Eleonora Ingrassia, Caterina Di Sano, Liboria Siena, Loredana Riccobono, Rosalia Gagliardo, Mirella Profita

“…We evaluated the ERK1/2 and IκBα phosphorylation, NF-κB signal pathway activation, ROS production, IL-8 and NOX-4 protein, and mRNA levels, in nasal epithelial cells RPMI 2650 stimulated with recombinant human (rh) IL-17A. …”
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Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue Metabolism by M. Arévalo-Turrubiarte, L. González-Dávalos, A. Yabuta, J. D. Garza, J. L. Dávalos, O. Mora, A. Shimada

“…No differences were found for PPARγ mRNA expression in SCAT. The results suggest the potential use of 2,4-TZD in beef cattle diets, because it improves AT differentiation, liver, and muscle fatty acid oxidation that, therefore, might improve energy efficiency.…”
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Curcumin and α/β-Adrenergic Antagonists Cotreatment Reverse Liver Cirrhosis in Hamsters: Participation of Nrf-2 and NF-κB by José Roberto Macías-Pérez, Bruno Jesús Vázquez-López, Martin Humberto Muñoz-Ortega, Liseth Rubí Aldaba-Muruato, Sandra Luz Martínez-Hernández, Esperanza Sánchez-Alemán, Javier Ventura-Juárez

“…In addition, carvedilol treatment and the combination of doxazosin with curcumin increased Nrf-2/NF-κB mRNA ratio and its protein expression in the inflammatory cells in the livers, possibly as another mechanism of hepatoprotection. …”
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ZFP36 Regulates Vascular Smooth Muscle Contraction and Maintains Blood Pressure by Xiuru Cui, Yawei Wang, Hanlin Lu, Lei Wang, Xianwei Xie, Shenghao Zhang, Pavel Kovarik, Shuijie Li, Shanshan Liu, Qunye Zhang, Jianmin Yang, Cheng Zhang, Jinwei Tian, Yan Liu, Wencheng Zhang

“…Zinc finger protein 36 (ZFP36) is an RNA‐binding protein that regulates mRNA stability by binding to adenylate‐uridylate‐rich elements in the mRNA 3′‐untranslated region. …”
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Structures and mechanisms of the RNA m⁶A writer by Deng Ting, Ma Jinbiao

“…N⁶-methyladenosine (m⁶A) is the most prevalent epigenetic modification found in eukaryotic mRNAs and plays a crucial role in regulating gene expression by influencing numerous aspects of mRNA metabolism. The m⁶A writer for mRNAs and long non-coding RNAs consists of the catalytic subunit m⁶A-METTL complex (MTC) (including METTL3/METTL14) and the regulatory subunit m⁶A-METTL-associated complex (MACOM) (including HAKAI, WTAP, VIRMA, ZC3H13, and RBM15/15B). …”
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Alternative RNA Splicing in Cardiac Diseases by Junli Gao, Yanan Zhang, Meiyu Hu, Tao He, Xiaohang Yin, Tian Hao, Guoping Li, Junjie Xiao

“…Alterations in exon exclusion, intron retention, or the selection of alternative splice sites modify transcript isoforms, which can consequently affect RNA molecules, including mRNA and non-coding RNAs, in terms of their localization, regulation, and overall function. …”
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A DATABASE ON TRANSLATIONAL ENHANCERS TO SUPPORT EXPERIMENTS WITH TRANSGENIC PLANTS by O. G. Smirnova, D. A. Rasskazov, A. V. Kochetov

“…A database on translational enhancers providing additional control of foreign gene expression at the mRNA translation level has been developed. It contains structured information on the presence of enhancers located within mRNAs, which control gene expression at the posttranscriptional stage. …”
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In Search of a Function for BCLAF1 by Haya Sarras, Solmaz Alizadeh Azami, J. Peter McPherson

“…We also present recent findings from our laboratory and others indicating a role for BCLAF1 in post-transcriptional processes that impact mRNA metabolism, instead of a direct role for this protein in apoptosis or transcription.…”
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A 20-Year-Old Man with IgA Vasculitis following COVID-19 Vaccination by Abdulaziz Alsubaie, Abdulmajeed Alshabanat, Abdulrahman Almizel, Mohammed Omair, Rahaf Alodaini

“…Herein, we report a case of a young man who is previously healthy and who developed IgA vasculitis after the first dose of the COVID-19 mRNA vaccine Pfizer-BioNTech. The patient’s symptoms were mainly skin and joint without renal or other system involvement. …”
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