Effects of Antipsychotic Drugs on the Epigenetic Modification of Brain-Derived Neurotrophic Factor Gene Expression in the Hippocampi of Chronic Restraint Stress Rats by Mi Kyoung Seo, Young Hoon Kim, Roger S. McIntyre, Rodrigo B. Mansur, Yena Lee, Nicole E. Carmona, Ah Jeong Choi, Gyung-Mee Kim, Jung Goo Lee, Sung Woo Park

“…Chronic restraint stress resulted in the downregulation of total and exon IV BDNF mRNA levels and a decrease in histone H3 acetylation and an increase in MeCP2 binding at BDNF promoter IV. …”
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The Transcription Factor SOX18 Inhibitor Small Molecule 4 Is a Potential Treatment of Cancer‐Induced Lymphatic Metastasis and Lymphangiosarcoma by Katja K. Koll, Martin M. Zimmermann, Patrick A. Will, Ulrich Kneser, Christoph Hirche

“…We found that Sox18 exhibited high mRNA expression levels in both HDLEC and MO‐LAS. …”
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miR-126 Mimic Counteracts the Increased Secretion of VEGF-A Induced by High Glucose in ARPE-19 Cells by Roberta Sanguineti, Alessandra Puddu, Massimo Nicolò, Carlo Enrico Traverso, Renzo Cordera, Giorgio L. Viviani, Davide Maggi

“…After 24 hours, medium was replaced and cells were cultured for 24 hours in normal (CTR) or diabetic condition (HG). Then, we analyzed mRNA levels of miR-126, VEGF-A, PI3KR2, and SPRED1. …”
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AICAR Protects against High Palmitate/High Insulin-Induced Intramyocellular Lipid Accumulation and Insulin Resistance in HL-1 Cardiac Cells by Inducing PPAR-Target Gene Expression by Ricardo Rodríguez-Calvo, Manuel Vázquez-Carrera, Luis Masana, Dietbert Neumann

“…Then, cells were exposed to AICAR stimulation for 24 h in the presence or absence of the AMPK inhibitor Compound C, and the mRNA levels of the three PPARs were analyzed by real-time RT-PCR. …”
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Construction of cuproptosis-related genes risk model predicts the prognosis of Uterine Corpus Endometrial Carcinoma by Yanfang Huang, Guoxian Luo, Xiujie Sheng, Jianqi Li

“…Validation of the selected genes, CDKN2A, GLS, and PPAT, was performed at both mRNA and protein levels. A total of 27 cuproptosis-related genes were identified, with 19 upregulated and 6 downregulated in UCEC. …”
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Inhibiting METTLl3-ATG5 axis-mediated harmful autophagy in macrophages could help reduce airway epithelial inflammation and remodeling in COPD by Chen Guiyang, Xia Zhiwei, Zeng Xianyou, Liu Hanying

“…Methylated RNA immunoprecipitation-qPCR showed that METTL3 knockdown reduced m6A, and the actinomycin D assay suggested that METTL3 knockdown reduced ATG5 mRNA levels and lowered ATG5 mRNA stability. METTL3-knockdown RAW264.7 reduced the inflammation and airway remodeling markers in the co-cultured mouse bronchial epithelial cells. …”
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Oncostatin M and TLR-4 Ligand Synergize to Induce MCP-1, IL-6, and VEGF in Human Aortic Adventitial Fibroblasts and Smooth Muscle Cells by David Schnittker, Karen Kwofie, Ali Ashkar, Bernardo Trigatti, Carl D. Richards

“…Signalling pathways including STATs, MAPKinases, and NFκB were activated, and LPS induced steady state mRNA levels of the OSM receptor chains OSMRβ and gp130. …”
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Dietary Solid-state-fermentation product of Bacillus velezensis T23 alleviate hepatic steatosis, oxidative stress, gut barrier damage, and microbiota dysbiosis in juvenile genetica... by Qiang Hao, Rolf Erik Olsen, Einar Ringø, Qianwen Ding, Tsegay Teame, Yuanyuan Yao, Chao Ran, Yalin Yang, Zhen Zhang, Zhigang Zhou

“…After 4 weeks of the feeding trial, the results showed that T23 supplementation notably reduced the hepatocytes vacuolization and the content of liver TAGs by downregulated the mRNA expression of the lipogenesis gene (fas), while upregulated the mRNA expression of lipid degradation genes (cpt1 and pparα) (p < 0.05). …”
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Exploring Future Pandemic Preparedness Through the Development of Preventive Vaccine Platforms and the Key Roles of International Organizations in a Global Health Crisis by Jihee Jeon, Eunyoung Kim

“…The need for rapid vaccine development has been highlighted by Coronavirus disease 2019 (COVID-19), and mRNA technology has shown promise as a platform. …”
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Dynamic Profile of CD4+ T-Cell-Associated Cytokines/Chemokines following Murine Myocardial Infarction/Reperfusion by Dongsheng Yuan, Jinjun Tie, Zhican Xu, Guanya Liu, Xinyu Ge, Zhulin Wang, Xumin Zhang, Shiyu Gong, Gang Liu, Qingshu Meng, Fang Lin, Zhongmin Liu, Huimin Fan, Xiaohui Zhou

“…IL-13 and MIP-1β showed higher levels in the MI/R serum at day 14 than at day 7. mRNA levels of IL-4, IL-6, IL-13, and IL-27 were increased in the day 7 group compared to the sham, while MIP-1β, MCP-3, and GRO-α mRNA levels showed no significant difference between the MI/R and sham groups in blood mononuclear cells. …”
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Creation of body color mutants by CRISPR/Cas9 gene editing in largemouth bass, Micropterus salmoides by Lina Sun, Tian Gao, Zhiqiang Li, Xiaolong Yang, Zuliang Qin, Mingxiao Ye, Ying Li, Feilong Wang, Deshou Wang

“…The efficiency of mutation was gRNA and Cas9 mRNA concentration dependent, and 400 ng/μl gRNA and 800 ng/μl Cas9 mRNA were recommended as the appropriate injection dose for genome editing in LMB. …”
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N-acetylcysteine increased the growth performance of Leuciscus waleckii by improving mitochondrial function, enhancing antioxidant and anti-inflammatory, and inhibiting apoptosis i... by Deng Lai Li, Rui Zhu, Si Tong Meng, Yin Tao Wang, Si Ying Liu, Zhi Yong Yang, Li Fang Wu

“…Meanwhile, 4.50 g/kg-6.00 g/kg NAC reduced the mRNA expression of hepatopancreatic and intestinal Bax, Caspase-3 and Caspase-9, and Caspase-3, Caspase-8 and Caspase-9 enzyme activities, and inhibited apoptosis. …”
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Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma by Hui Du, Yonghong Wang, Yumin Shi, Jian Yu, Wen Sun, Yiqun Zhang

“…Real-time PCR was used to determine the mRNA expression levels of interleukin- (IL-) 10, IL-17, matrix metalloproteinase-9 (MMP-9), and transforming growth factor β1 (TGF-β1) in peripheral blood mononuclear cells before and after treatment. …”
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Combined Effects of Chrysin Supplementation and Exercise Training on Diabetes-Induced Oxidative Stress and Apoptosis in Rat Testicular Tissue by Shiva Cheraghi Abajlou, Asghar Tofighi, Javad Tolouei Azar, Amir Afshin Khaki, Mazdak Razi

“…Following 8 weeks, the histopathological changes (Johnson’s score, epithelial height,and tubular diameter), testicular malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase(GPX), and the mRNA levels of anti-apoptotic gene Bcl-2 and pro-apoptotic gene Bax was analyzed.Results: Chrysin solely and simultaneous with MIRET could remarkably (P=0.001) improve the DM-induced histopathologicaldamages, increase the testicular SOD and GPx levels, and decline the DM-increased MDA content.Moreover, our results showed that Chrysin solely and more simultaneously with MIRET could significantly (P=0.001)decrease the mRNA expression of Bax and improve the Bcl-2 expression and rebalance the Bax/Bcl-2 balance.Conclusion: Our findings showed that co-administration of Chrysin along with MIRET can significantly amelioratethe DM-induced histopathological, and biochemical impairments and reduce the pro-apoptotic impact of DMon testicular tissue.…”
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Polarization of ILC2s in Peripheral Blood Might Contribute to Immunosuppressive Microenvironment in Patients with Gastric Cancer by Qingli Bie, Pan Zhang, Zhaoliang Su, Dong Zheng, Xinyu Ying, Yumin Wu, Huijian Yang, Deyu Chen, Shengjun Wang, Huaxi Xu

“…The increased frequency of ILC2s was also found in PBMC of patients by flow cytometry. In addition, the mRNA of Arg1 and iNOS were also significantly increased in patients. …”
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Comprehensive Analysis of Differentially Expressed miRNAs and mRNAs Reveals That miR-181a-5p Plays a Key Role in Diabetic Dermal Fibroblasts by Peng Liu, Yi Zhu, Qin Li, Biao Cheng

“…We searched the GEO database for related miRNA (GSE68185, GSE84971) and mRNA (GSE49566, GSE78891) profiles. After eliminating batch effects and identifying differentially expressed genes (DEGs), we applied enrichment analyses and found that 3 miRNAs and 30 mRNAs were differentially expressed in diabetic HDFs. …”
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Dose-dependent role of AMH and AMHR2 signaling in male differentiation and regulation of sex determination in Spotted knifejaw (Oplegnathus punctatus) with X1X1X2X2/X1X2Y chromosom... by Haixia Zhao, Jun Li, Zhizhong Xiao, Yongshuang Xiao

“…Notably, the specific mRNA expression of amhr2 is significantly enriched in amhr2cy, whose extracellular domain exhibits the highest binding affinity for Amh protein, with sexual expression differences manifesting as early as 5 dph. …”
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Serum Glial Cell Line-Derived Neurotrophic Factor (sGDNF) Is a Novel Biomarker in Predicting Cirrhosis in Patients with Chronic Hepatitis B by Guangyue Yang, Liping Zhuang, Tiantian Sun, Yee Hui Yeo, Le Tao, Wei Zhang, Wenting Ma, Liu Wu, Zongguo Yang, Yanqin Yang, Dongying Xue, Jie Zhang, Rilu Feng, Ebert Matthias P., Steven Dooley, Ekihiro Seki, Ping Liu, Cheng Liu

“…Conclusion. Using serum, tissue mRNA, and biopsy data, our study revealed a significant potential of sGDNF as a novel noninvasive biomarker for cirrhosis in CHB patients.…”
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Spontaneous tumor regression following COVID-19 vaccination by Hui Dai, Renata Ferrarotto, Myrna Godoy, Diana Bell, Shiaw-Yih Lin, Daniel J McGrail, Luana Guimaraes de Sousa, Kaiyi Li, Mario L Marques-Piubelli, Cipriano Gonzalez, Sammy Ferri-Borgogno, Jared Burks

“…Insight: This case suggests that the mRNA-1273 COVID-19 vaccine stimulated anticancer immunity and tumor regression.…”
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Triiodothyronine (T3) increases the expression of the amphiregulin (AREG) oncogene by activating extranuclear pathways in MCF-7 breast cancer cells by Maria Teresa De Sibio, Fernanda Cristina Fontes Moretto, Regiane Marques Castro Olimpio, Miriane de Oliveira, Lucas Solla Mathias, Vinícius Vigliazzi Peghinelli, Helena Paim Tilli, Bianca Mariani Gonçalves, Dariane Beatriz Marino Cardoso, Larissa Silva Dall Aqua, Igor de Carvalho Depra, Mariana Menezes Lourenço, Aline Carbonera Luvizon, Paula de Oliveira Montandon Hokama, Maria Tereza Nunes, Marna Eliana Sakalem, Célia Regina Nogueira

“…A control group (C) received no T3 or inhibitors. Analyses of mRNA and protein expression were done using RT-qPCR and Western blot, respectively. …”
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