The methyl-CpG binding domain 2 regulates peptidylarginine deiminase 4 expression and promotes neutrophil extracellular trap formation via the Janus kinase 2 signaling pathway in e... by Biao Peng, Mu-Yun Yan, Yun-Rong Chen, Fei Sun, Xu-Dong Xiang, Da Liu

“…In both severe asthma mice and HL-60-differentiated neutrophil-like cells in vitro, inhibiting MBD2 reduced the mRNA and protein expression of JAK2 and PAD4, and inhibiting JAK2 reduced the expression of PAD4 mRNA and protein.Conclusion MBD2 regulates PAD4 expression through the JAK2 signaling pathway to promote NETs formation in mice with severe asthma. …”
Get full text

CCAAT/Enhancer-Binding Protein β Mediates Oxygen-Induced Retinal Neovascularization via Retinal Vascular Damage and Vascular Endothelial Growth Factor by Tingting Li, Xuan Cai, Xiangning Wang, Xueyan Zhang, Hui Zhang, Biwei Xu, Shiwei Li, Jianyan Hu, Qiang Wu

“…Reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analyses were used to measure mRNA and protein levels of C/EBP β and vascular endothelial growth factor (VEGF). …”
Get full text

Complement Receptors C5aR and C5L2 Are Associated with Metabolic Profile, Sex Hormones, and Liver Enzymes in Obese Women Pre- and Postbariatric Surgery by Reza Rezvani, Jessica Smith, Marc Lapointe, Picard Marceau, Andre Tchernof, Katherine Cianflone

“…Over 12 months postoperatively, liver enzymes in low C5L2/C5aR mRNA ratio group remained higher (ALP and ALT, P<0.05, AST and GGT, P<0.001 2-way-ANOVA). …”
Get full text

SLC1A5 is a key regulator of glutamine metabolism and a prognostic marker for aggressive luminal breast cancer by Lutfi H. Alfarsi, Rokaya El Ansari, Busra Erkan, Ali Fakroun, Madeleine L. Craze, Mohammed A. Aleskandarany, Kiu Wai Cheng, Ian O. Ellis, Emad A. Rakha, Andrew R. Green

“…In large BC cohorts, SLC1A5 mRNA (n = 9488) and SLC1A5 protein (n = 1274) levels were assessed and correlated their expression with clinicopathological features, molecular subtypes, and patient outcomes. …”
Get full text

The Impact of the Coexpression of MET and ESR Genes on Prognosticators and Clinical Outcomes of Breast Cancer: An Analysis for the METABRIC Dataset by Nehad M. Ayoub, Ghaith M. Al-Taani, Amer E. Alkhalifa, Dalia R. Ibrahim, Aymen Shatnawi

“…The ESR1 mRNA expression was significantly different among MET CNAs groups p<0.001. …”
Get full text

Gene Expression of IGF1, IGF1R, and IGFBP3 in Epiretinal Membranes of Patients with Proliferative Diabetic Retinopathy: Preliminary Study by Dorota Romaniuk, Malgorzata W. Kimsa, Barbara Strzalka-Mrozik, Magdalena C. Kimsa, Adam Kabiesz, Wanda Romaniuk, Urszula Mazurek

“…Therefore, the present study focused on the assessment of IGF1, IGF1R, and IGFBP3 mRNA levels in ERMs and PBMCs from patients with PDR. …”
Get full text

Insulin Detemir Causes Lesser Weight Gain in Comparison to Insulin Glargine: Role on Hypothalamic NPY and Galanin by Mohammad Ishraq Zafar, Cuining Hu, Danfeng Liu, Raja Adeel Shafqat, Feng Gao

“…The expression of hypothalamic NPY and GAL at both mRNA and protein level were significantly lower (P<0.05) in the DM+DE group. …”
Get full text

Modifying Graft-versus-Host Disease in a Humanized Mouse Model by Targeting Macrophages or B-Cells by Marieke C. H. Hogenes, Suzanne van Dorp, Joyce van Kuik, Filipa R. P. Monteiro, Natalie ter Hoeve, Liane Guedes, Marijke R. van Dijk, Anton C. Martens, Roel A. de Weger

“…This may be related to a difference in the mRNA levels of TGF-β. Also, lower mRNA levels of Tregs in some organs were observed after Rituximab treatment, which contradicts that a higher number of Tregs would always be related to less severe GvHD. …”
Get full text

Pioglitazone Attenuates Vascular Fibrosis in Spontaneously Hypertensive Rats by Dengfeng Gao, Ning Ning, Guanghua Hao, Xiaolin Niu

“…Masson’s trichrome staining for collagen III and real-time RT-PCR analysis of collagen I, III and fibronectin mRNA indicated that pioglitazone significantly inhibited extracellular matrix production in the aorta. …”
Get full text

BioLake: an RNA expression analysis framework for prostate cancer biomarker powered by data lakehouse by Qiaowang Li, Yaser Gamallat, Jon George Rokne, Tarek A. Bismar, Reda Alhajj

“…In summary, BioLake offers a lightweight online tool to facilitate bioinformatic mRNA data analysis with the support of custom online data processing.…”
Get full text

Prophylactic effect of Kudingcha polyphenols on oxazolone induced colitis through its antioxidant capacities by Xingyao Long, Yanni Pan, Xin Zhao

“…Keywords: Kudingcha polyphenols, Oxazolone, Antioxidant, Colitis, mRNA expression…”
Get full text

Dulaglutide Protects Mice against Diabetic Sarcopenia-Mediated Muscle Injury by Inhibiting Inflammation and Regulating the Differentiation of Myoblasts by Fengyi Deng, Wenyan Wu, Xingyu Fan, Xing Zhong, Nuojin Wang, Yue Wang, Tianrong Pan, Yijun Du

“…High glucose inhibited the differentiation of C2C12 cells and decreased the mRNA and protein levels of myokines. Dulaglutide could reverse the differentiation state induced in C2C12 cells by high glucose.…”
Get full text

Effects of Tocotrienol on Cardiovascular Risk Markers in Patients With Chronic Kidney Disease: A Randomized Controlled Trial by Liana Trugilho, Lívia Alvarenga, Ludmila Cardozo, Bruna Paiva, Jessyca Brito, Isis Barboza, Jonatas Almeida, Juliana dos Anjos, Pramod Khosla, Marcelo Ribeiro-Alves, Denise Mafra

“…Transcription factors NRF2 and NF-κB mRNA expressions remained unaltered in both groups. …”
Get full text

Cyanin Chloride Inhibits Hyperbaric Pressure-Induced Decrease of Intracellular Glutamate-Aspartate Transporter in Rat Retinal Müller Cells by Xiaomin Chen, Yue Wang, Fangfang Han, Min Ke

“…Cell survival rates (SRs) were evaluated by an MTT assay. GLAST mRNA and protein expression were determined by western blot and RT-PCR analyses, respectively. …”
Get full text

Transient degradation of NF-κB proteins in macrophages after interaction with mast cell granules by Noriko Ito, Yuai Li, Tsuneo Suzuki, Daniel J. Stechschulte, Kottarappat N. Dileepan

“…On the other hand, in peritoneal macrophages, MCG induce more extensive modifications to NF-κB proteins with associated inhibition of iNOS or TNF-α mRNA expression.…”
Get full text

Functional analysis of a novel homozygous missense IVD gene variant: a case report with dual genetic diagnoses by Yuying Zhu, Ke Wu, Hanying Wen

“…The assays of relative IVD gene mRNA expression, IVD protein expression, and enzymatic activity were used.ResultsWhole-exome sequencing identified a novel homozygous missense variant in the IVD gene (NM_002225.5) c.1006T&gt;C (p.Cys336Arg) within a region of homozygosity of 15q11.2-q21.3. …”
Get full text

Modeling the endocrine control of vitellogenin production in female rainbow trout by Kaitlin Sundling, Gheorghe Craciun, Irvin Schultz, Sharon Hook, James Nagler, Tim Cavileer, Joseph Verducci, Yushi Liu, Jonghan Kim, William Hayton

“…Numerical simulation results based on this model are comparedwith experimental data for estrogen receptor mRNA, Vg mRNA, and Vgin the plasma from female rainbow trout over a normal annual reproductivecycle. …”
Get full text

Effects of Aspirin on Odontogenesis of Human Dental Pulp Cells and TGF-β1 Liberation from Dentin In Vitro by V. Khampatee, C. Zhang, L. Chou

“…Expressions of DSP and RUNX2 were determined with the ELISA. DSP and RUNX2 mRNA levels were measured with RT-qPCR. Alizarin red staining was conducted to evaluate the mineralized nodule formation. …”
Get full text

A Study Comparing Immunohistochemistry, Fluorescence in situ hybridization, GeneXpert® Breast Cancer STRAT4 Assay for Determining Hormone Receptor, Human Epidermal Growth Factor Re... by Rajaa El Aje, Abdellah Naya, Ayoub Khoaja, Younes Zaid, Mounia Oudghiri, Jodi Weidler, Mehdi Karkouri

“…The simple Kappa coefficient was, for ER, 0.830 (P < 0.0001), 0.565 (P < 0.0001) for PR, 0.838 (P < 0.0001) for HER2-IHC, 0.771 (P < 0.0001) for HER2 IHC+FISH and, for, Ki67, 0.458 (P < 0.0001); Conclusions: We demonstrated globally a high concordance between centrally assessed IHC, IHC+FISH and mRNA measurements of ER/ESR1 and HER2/ERBB2, and a moderate agreement between PR/PGR and Ki67/MKi67. …”
Get full text

Normal Human Gingival Epithelial Cells Sense C. parapsilosis by Toll-Like Receptors and Module Its Pathogenesis through Antimicrobial Peptides and Proinflammatory Cytokines by Raouf Bahri, Sèverine Curt, Dalila Saidane-Mosbahi, Mahmoud Rouabhia

“…Following contact with C. parapsilosis, normal human gingival epithelial cells expressed high levels of Toll-like receptors (TLR)-2, -4, and -6, but not TLR-9 mRNA. The upregulation of TLRs was paralleled by an increase of IL-1β, TNFα, and IFNγ mRNA expression, suggesting the involvement of these cytokines in the defense against infection with C. parapsilosis. …”
Get full text