“Brick‐Mortar‐Binder” Design toward Highly Elastic, Hydrophobic, and Flame‐Retardant Thermal Insulator by Shanying Sui, Huafeng Quan, Jingxing Wang, Yufang Lu, Yufan Yang, Yuhan Sheng, Zhifang Sun, Yi Zhang

“…These distinctive features make the LCS hybrid aerogel highly promising for thermal management applications in extreme conditions, such as in pipelines for transporting liquid nitrogen and liquefied natural gas.…”
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Analysis and optimization of LN2 two-phase flow in CRAFT NNBI cryopump by Qianxu Wang, Yuanlai Xie, Huihui Hong, Yang Zhu, Fang Wang, Kun Tian, Bin Li

“…As an important component of Negative ion based Neutral Beam Injector (NNBI), the cryopump mainly provides a suitable vacuum environment for beam generation and transmission. In the paper, Liquid nitrogen (LN2) pipe structure models of two cryopumps were established for simulation and experimental studies. …”
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Bacterial Isolation from Natural Grassland on Nitrogen-Free Agar Yields Many Strains Without Nitrogenase by Amrit Koirala, Nabilah Ali Alshibli, Bikram K. Das, Volker S. Brözel

“…Putative nitrogen fixers were confirmed by subculturing in liquid nitrogen-free media and PCR amplification of the <i>nifH</i> genes. …”
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A modified droplet vitrification method for cryopreservation of shoot tips from <i>in vitro</i> potato plants by T. A. Gavrilenko, N. A. Shvachko, N. N. Volkova, Yu. V. Ukhatova

“…Panis and colleagues: 1) preparation of plant material, 2) isolation of shoot tips, 3) treatment of explants with cryoprotector solutions, 4) freezing/immersion in liquid nitrogen, 5) thawing, 6) post-cryogenic recovery and evaluation of viability and regeneration capacity. …”
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RED light promotes flavonoid and phenolic accumulation in Cichorium spp. callus culture as anti-candida agent by Sayeda Abdelrazek Abdelhamid, Alaa I. Marzouk, Mohsen S. Asker, Hattem M. El Shabrawi

“…After extraction using liquid nitrogen and methanol, the resulting callus extracts were tested against Candida albicans NRRL477 at various concentrations (1/8, 1/4, and 1/2 MIC) for 20 to 120 min. …”
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Germination and establishment of Teramnus labialis (L.f.) Spreng plants are affected by scarification treatment used by Yanier Acosta, Sershen, Jorge A. Sánchez, Inaudis Cejas, Dayamí Fontes, Marcos E. Martínez-Montero

“…Methods: Four scarification treatments (sanding, sulfuric acid, hot water, and liquid nitrogen (LN)) were evaluated under controlled laboratory and field conditions. …”
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FTIR: A novel method for identifying fish gut contents by Abolfazl Aminimehr, Mohammad Amini Chermahini, Laleh Moosavi Dehmoordi

“…The feces were collected from the end of the intestine, preserved in liquid nitrogen, then freeze-dried and homogenized. The samples were analyzed using the ATR-FTIR.Results and discussion: By comparing the obtained wavelengths, specific patterns were identified between the ingested food and the resulting feces. …”
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A Comparative Analysis of the Antioxidant Profiles Generated by the RoXsta^TM System for Diverse Biological Fluids Highlights the Powerful Protective Role of Human Semina... by Robert J. Aitken, Alexandra Wilkins, Natasha Harrison, Mohammad Bahrami, Zamira Gibb, Kaitlin McIntosh, Quan Vuong, Sarah Lambourne

“…The powerful antioxidant properties of human semen were shown to reside within the seminal plasma (SP) fraction, rather than the spermatozoa, and to be resistant to snap freezing in liquid nitrogen. Moreover, comparative studies demonstrated that human SP exhibited significantly (<i>p</i> < 0.001) higher levels of antioxidant potential than any other species examined (stallion, bull, dog) and that this intense activity reflected the relative vulnerability of human spermatozoa to peroxide attack. (4) Conclusions: The RoXsta^TM system provides valuable information on the antioxidant profile of complex biological fluids, supporting its diagnostic role in conditions associated with oxidative stress. …”
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Comparison of the Efficacy and Safety of Two Cryotherapy Protocols in the Treatment of Common Viral Warts: A Prospective Observational Study by Jihan M. Muhaidat, Firas A. Al-qarqaz, Diala M. Alshiyab, Hadeel S. Alkofahi, Yousef Khader, Mawaddah Y. Ababneh

“…Cryotherapy (freezing by liquid nitrogen) is an effective and widely used method for treatment of common warts. …”
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Investigation of the post-cryogenic regeneration ability of potato varieties under different cultivation conditions by E. S. Bespalova, Yu. V. Ukhatova, N. N. Volkova, E. V. Oves, N. A. Gaitova, T. A. Gavrilenko

“…This paper presents the results of studying the effect of cultivation conditions after plunging into liquid nitrogen and thawing of shoots tips and axillary buds of in vitro plants on their postcryogenic recovery. …”
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A “Novel” Protocol for the Analysis of Hydroxycinnamic Acids in Leaf Tissue of Chicory (Cichorium intybus L., Asteraceae) by Meriem Bahri, Philippe Hance, Sébastien Grec, Marie-Christine Quillet, Francis Trotin, Jean-Louis Hilbert, Theo Hendriks

“…Comparisons with extractions of liquid nitrogen powders indicated that the novel extraction method was reliable. …”
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A novel cryopreservation solution for adipose tissue based on metformin by Yaping Deng, Xin Liu, Xichao Jian, Yan Zhang, Yinchi Hou, Suyun Hou, Fang Qi, Shune Xiao, Chengliang Deng

“…Samples were cryopreserved in liquid nitrogen for 2 weeks. After thawing, 1 mL of adipose tissue from each group was transplanted subcutaneously into the backs of nude mice. …”
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A simple and efficient method to extract polar metabolites from guar leaves (Cyamopsis tetragonoloba (L.) Taub.) for GC-MS metabolome analysis by S. B. Teplyakova, A. L. Shavarda, T. V. Shelenga, E. A. Dzyubenko, E. K. Potokina

“…The essence of the new method is that the leaf is rapidly frozen in liquid nitrogen with subsequent thawing in cold methanol. …”
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Crocin improves post-thaw quality, fertility-associated gene expression and fertilization potential of buffalo bull sperm by Amjad Riaz, Ghulam S. Khan, Muhammad Zahid Tahir, Muhammad Yasir Zahoor, Hifz-ul-Rahman Hifz-ul-Rahman

“…The extended semen was packed in 0.5 mL French straws (25 x 10 6 sperm/ straw) and cryopreserved using liquid nitrogen vapor freezing protocol. Computer-assisted semen analysis, Normal Apical Ridge assay, Rhodamine 123, Acridine orange, and Propidium iodide staining were used to assess sperm motility parameters, acrosome integrity, mitochondrial membrane potential, DNA integrity, and viability, respectively. …”
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Water homeostasis gene expression in the kidney of broilers divergently selected for water conversion ratio by Kentu Lassiter, Loujain Aloui, Elizabeth S. Greene, Marciela Maqaeda, Travis Tabler, Sami Dridi, Robert F. Wideman, Jr., Sara Orlowski, Walter G. Bottje

“…Kidney samples were collected, flash frozen in liquid nitrogen, and kept at -80 °C for gene expression analysis. …”
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Quinazolinone Derivative MR2938 Protects DSS-Induced Barrier Dysfunction in Mice Through Regulating Gut Microbiota by Ling Lv, Mireguli Maimaitiming, Jichen Yang, Shuli Xia, Xin Li, Pingyuan Wang, Zhiqing Liu, Chang-Yun Wang

“…Cecal content was also preserved in liquid nitrogen and subsequently analyzed following 16S RNA sequencing. …”
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