Concepts, History, Principles, and Application of Germplasm Cryopreservation Technology by Huiping Yang, Terrence R. Tiersch

“…Cryopreservation refers to the preservation of biological materials at extremely low temperatures, typically using solid carbon dioxide at -80°C or liquid nitrogen at -196°C for freezing, and cryogenic storage in perpetuity. …”
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Concepts, History, Principles, and Application of Germplasm Cryopreservation Technology by Huiping Yang, Terrence R. Tiersch

“…Cryopreservation refers to the preservation of biological materials at extremely low temperatures, typically using solid carbon dioxide at -80°C or liquid nitrogen at -196°C for freezing, and cryogenic storage in perpetuity. …”
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Study on Pore and Fissure Structure Characteristics of Deep Soft Coal Rock by Minbo Zhang, Zhen Zhang, Dangyu Zhang, Delong Zou, Jinlei Du, Zichao Wang, Chunxin Li

“…According to the results of the low-temperature liquid nitrogen adsorption experiment, the hysteresis curves of coal samples can be divided into two types. …”
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Viability of pollen in sweet cherry (<i>Cerasus avium</i>) varieties of different ecogeographic origin in the Northwestern region of Russia by S. Yu. Orlova, A. V. Pavlov, V. G. Verzhuk

“…Pollen viability parameters (length of pollen tubes and percentage of germinated pollen grains) positively correlated prior to immersion into liquid nitrogen from r = 0,54 (gr. III) to r = 0,76 (gr. …”
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The INRAE Biological Resource Center ‘BrACySol’: a French centre of valuable Brassica, Allium and Solanum genetic resources for breeding by Florence Esnault, Marie-Pierre Cann, Jean-Eric Chauvin, Anne-Marie Chèvre, Marie-Ange Dantec, Stéphane Doré, Pascal Glory, Marie-Claire Kerlan, Dominique Kermarrec, Anne Laperche, Lise-Anna Le Ven, Maria Manzanares-Dauleux, Roland Pellé, Jocelyne Porhel, Julien Quéran, Vincent Richer, Catherine Souchet, Sylvain Théréné, Mathieu Tiret, Nathalie Nési

“…The Brassica genetic resources are conserved as seeds in freezers or liquid nitrogen. The Allium resources are maintained by vegetative propagation in fields or greenhouses and the Solanum resources are maintained by vegetative propagation in fields, greenhouses, in vitro or in liquid nitrogen. …”
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LOW TEMPERATURE STORAGE OF ACTINOBACILLUS PLEUROPNEUMONIAE CULTURES by V. S. Rusaleyev, D. A. Vasilyev

“…Experimental data are reported that were obtained during research of potential effect of liquid nitrogen freezing on vitality and some biological properties of actinobacilli. …”
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Combined multi-scale characterization of pores in ultra-thick coal seams of Jurassic Xishanyao Formation, Tiaohu-Malang sags, Santanghu Basin by Yue CHEN, Qiqi LEI, Dongmin MA, Xin WANG, Xinggang WANG, Diefang HUANG, Gaoxiang RONG

“…The fractal characteristics of the pore structures also differ significantly between the two coal seams, with the 9-1 coal sample showing stronger heterogeneity. Its liquid nitrogen adsorption curves correspond to type Ⅱ with a H4 hysteresis loop. …”
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Coal Reservoir Characterization in a Tectonic Setting and the Effects of Tectonism on the Coalbed Methane (CBM) Content by Zhenni Ye, Enke Hou, Zhonghui Duan, Zengjiang Li

“…In this study, micropore-microfracture structural parameters of coal samples from the Guojiahe coalfield are determined through the use of X-ray photography, an image recognition algorithm, and a liquid nitrogen adsorption method. The relationship between the micropore-microfracture characteristics of the reservoir and the gas content is quantitatively assessed using the Grassberger and Procaccia (GP) algorithm to calculate the correlation dimension of the parameters. …”
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Cryosurgery as an Option for the Treatment of Vascular Lesions of the Oral Cavity by Pedro Thalles Bernardo de Carvalho Nogueira, Mariana Maria Castro Jatobá Remigio, Andreza Maria Correia de Queiroz, Andréia Aparecida da Silva, José Rodrigues Laureano Filho

“…We report the case of a patient with a hemangiomatous lesion of the oral cavity who was treated with liquid nitrogen spray cryosurgery, with successful results at 18-month follow-up.…”
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The Value of Cryosurgery in Treating a Case of Thoracic Chondrosarcoma by Kalliopi Alpantaki, George Datsis, Odysseas Zoras, Alkisti Kampouroglou, Ioannis Drositis, George Halkiadakis, Pavlos Katonis

“…Our patient was treated in two stages, with total excision of the tumor, using cryosurgery. Liquid nitrogen was used to freeze the damaged tissue at a cellular level and made the excision more efficient.…”
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The analysis of apricot and sweet cherry pollen viability after cryopreservation by M. N. Sitnikov, V. G. Verzhuk, A. V. Pavlov, D. D. Bondaruk

“…The possibility of long-term pollen preservation in liquid nitrogen vapor is shown. Based on the results obtained, a collection of pollen is being created for implementing various breeding programs.…”
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EFFECTIVE SILICON DEVICE STRUCTURES WITH RADIATIVE RECOMBINATION ON DISLOCATIONS by A. V. Mudryi, V. D. Zhivulko, F. Mofidnakhaei, G. D. Ivlev, M. V. Yakushev, R. W. Martin, A. V. Dvurechenskii, V. A. Zinovyev, Zh. V. Smagina, P. A. Kuchinskaja

“…The efficient electroluminescence in the region of band-to-band (1,1 eV) and dislocationrelated (D1 – 0,8 eV) transitions has been detected from Si p-n structures at room and liquid nitrogen temperatures. It was found that dislocation-related luminescence in Si single crystals is considerably stronger than the intrinsic band-to-band emission in the wide temperature range of 4,2–300 K. …”
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Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples by Namrata Ghag, Joshua Tam, Rox Anderson, Nashwa Cheema

“…Freezing samples in liquid nitrogen, using isopentane as a mediator for efficient cooling, preserves the tissue in its natural state. …”
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Examine the Mechanical Properties of Aluminium Tetrahydride/Calotropis gigantea Based Hybrid Polyester Composites in Cryogenic Atmosphere by Pavan Balappa Bagali, N. I. Haroon Rashid, Essam A. Al-Ammar, C. Srinivas, H. V. Jayaprakash, J. D. Venkatesh, Sohail M. A. K. Mohammed, Melvin Victor De Poures, Nahom Adugna

“…The materials with 5 wt proportions of filler, 3 layers of CGF and 30 min of liquid nitrogen treatment (A2, B3 and C2) showed better mechanical strength. …”
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Development of Ground Freezing System for Undisturbed Sampling of Granular Soils by Youngseok Kim, Bumsik Hwang, Wanjei Cho

“…The applied coolant was liquid nitrogen and circulated through a double tube inserted in the ground. …”
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Study on the effect of acid fracturing fluid on pore structure of middle to high rank coal by Zhao Kang, Li Liangwei, Li Kun, Zuo Shaojie, Jiang Zhizhong

“…High-pressure mercury intrusion and liquid nitrogen adsorption techniques results demonstrated that the acid fracturing fluid can effectively alter the pore structure of coal. …”
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Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench by Balciunaite Gabriele, Juodsnukyte Jovita, Savickas Arunas, Ragazinskiene Ona, Siatkute Luka, Zvirblyte Gitana, Mistiniene Edita, Savickiene Nijole

“…Moench after homogenization of roots with liquid nitrogen, extraction in 0.01 mol L-1 phosphate-buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. …”
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Recycling of the rare earth oxides from spent rechargeable batteries using waste metallurgical slags by Tang K., Ciftja A., van der Eijk C., Wilson S., Tranell G.

“…The spent battery modules were first frozen with liquid nitrogen for the de-activation and brittle fracture treatment. …”
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Liquid and Frozen Storage of Agouti (Dasyprocta leporina) Semen Extended with UHT Milk, Unpasteurized Coconut Water, and Pasteurized Coconut Water by W. M. Mollineau, A. O. Adogwa, G. W. Garcia

“…Experiment 2: 150 ejaculates were extended for storage as frozen pellets in liquid nitrogen at −195∘C, thawed at 30∘ to 70∘C for 20 to 50 seconds after 5 days and evaluated for FPM% and its rate of deterioration. …”
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Induction of somatic embryogenesis and cryopreservation of Abies pinsapo Boiss. by Laura Cabero-Moreno, Ignacio Landeras-López, María Victoria Bravo-Navas, Carolina Sánchez-Romero

“…Following fluorescein diacetate (FDA) staining, more intense and abundant green fluorescence could be observed in these samples, compared to those subjected to other preconditioning and cryoprotective treatments, thus evincing a higher proportion of viable cells after freezing in liquid nitrogen. Cold hardening did not improve cryotolerance. …”
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