Chemical characterization of Siraitia grosvenorii granules and their efficacy and mechanism of action on PM2.5-induced acute lung injury by Qi Shao, Yan Zhao, YuYu Shi, Fafeng Cheng, ZeHan Zhang, Ying Liu, ChangXiang Li, ZiLin Ren, Hua Bai, Haojie Cheng, Rolando Maddela, Junqiang Tian, Xueqian Wang

“…The chemical composition of SGG, such as wogonin (MOL000173), luteolin (MOL000006), nobiletin (MOL005828), naringenin (MOL004328), acacetin (MOL001689), were identified via ultra-high-performance liquid chromatography-Q Exactive (UHPLC-QE) Orbitrap/MS. …”
Get full text

Transcripts of Anthocyanidin Reductase and Leucoanthocyanidin Reductase and Measurement of Catechin and Epicatechin in Tartary Buckwheat by Yeon Bok Kim, Aye Aye Thwe, YeJi Kim, Xiaohua Li, Jin Woong Cho, Phun Bum Park, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Sun-Ju Kim, Tastsuro Suzuki, Kwang Hyun Jho, Sang Un Park

“…Gene expression was carried out by quantitative real-time RT-PCR, and catechin and epicatechin content was analyzed by high performance liquid chromatography. The expression pattern of ANR and LAR did not match the accumulation pattern of PAs in different organs of two cultivars. …”
Get full text

Fabrication of taxifolin loaded zein-caseinate nanoparticles and its bioavailability in rat by Ying Li, Hang Su, Wenjun Wang, Zhongping Yin, Jing’en Li, En Yuan, Qingfeng Zhang

“…After oral administration, taxifolin and its five metabolites were identified in rat plasma by ultra high performance liquid chromatography (UPLC) with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). …”
Get full text

Peritoneal Nebulization of Ropivacaine during Laparoscopic Cholecystectomy: Dose Finding and Pharmacokinetic Study by Massimo Allegri, Martina Ornaghi, Catherine E. Ferland, Dario Bugada, Yash Meghani, Serena Calcinati, Manuela De Gregori, Federica Lovisari, Krishnaprabha Radhakrishnan, Maria Cusato, Stefano Scalia Catenacci, Marta Somaini, Guido Fanelli, Pablo Ingelmo

“…The pharmacokinetics of ropivacaine was evaluated using high performance liquid chromatography. Results. Nebulization of 50 mg of ropivacaine had the same effect of 100 or 150 mg in terms of postoperative morphine consumption, shoulder pain, postoperative nausea and vomiting, activity resumption, and hospital discharge timing (>0.05). …”
Get full text

Stability-Indicating HPLC Assay for Determination of Idebenone in Pharmaceutical Forms by Sonoube Kombath, Issa-Bella Balde, Sandra Carret, Sofiane Kabiche, Salvatore Cisternino, Jean-Eudes Fontan, Joël Schlatter

“…The assay was achieved by liquid chromatography analysis using a reversed-phase C18 column and a detector set at 480 nm. …”
Get full text

Pharmacokinetic Analysis of Diosgenin in Rat Plasma by a UPLC-MS/MS Approach by Pei Liu, Lin Xu, Jing-han Guo, Jin-hua Chang, Xi-gang Liu, He-fei Xue, Ru-xing Wang, Zhong-si Li, Guang-xin Miao, Cui-zhe Liu, Jian-yu Zhou

“…The aim of the present study was to establish an efficient, sensitive ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) approach for pharmacokinetic analysis of diosgenin amorphous solid dispersion (ASD) using tanshinone IIA as an internal standard (IS). …”
Get full text

Global metabolomic profiling of tumor tissue and paired serum samples to identify biomarkers for response to neoadjuvant FOLFIRINOX treatment of human pancreatic cancer by Manoj Amrutkar, Sander Johannes Thorbjørnsen Guttorm, Anette Vefferstad Finstadsveen, Knut Jørgen Labori, Lars Eide, Helge Rootwelt, Katja Benedikte Prestø Elgstøen, Ivar P. Gladhaug, Caroline S. Verbeke

“…To address this unmet need, global metabolomic profiling was performed on tumor tissue and paired serum samples from patients with treatment‐naïve (TN; n = 18) and neoadjuvant leucovorin calcium (folinic acid), fluorouracil, irinotecan hydrochloride and oxaliplatin (FOLFIRINOX)‐treated (NAT; n = 17) PDAC using liquid chromatography mass spectrometry. Differentially abundant metabolites (DAMs) in TN versus NAT groups were identified and their correlation with various clinical parameters was assessed. …”
Get full text

Exploring Adsorption Performance of Functionalized Mesoporous Silicas with a Different Pore Structure as Strong Cation-Exchange Sorbents for Solid-Phase Extraction of Atropine and... by Fernando L. Vera-Baquero, Sonia Morante-Zarcero, Damián Pérez-Quintanilla, Isabel Sierra

“…Analyses were carried out by ultra-high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry. …”
Get full text

Identification of compounds against atherosclerosis induced by ox-LDL based on cell extraction/UPLC–MS/MS from mulberry twigs and their mechanistic analysis by Zhihui Jin, Wei Xiang, Xiaowen Shi, Fenglin Jiang, Yanan Jia, Yuansong Zhang, Lingshu Zeng, Xianzhi Huang, Li Xu

“…Methods Biospecific cell extraction and ultra-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) were employed to screen and identify these compounds. …”
Get full text

In vitro and in silico analysis of dual-function peptides derived from casein hydrolysate by Maolin Tu, Xinyu Qiao, Cong Wang, Hanxiong Liu, Shuzhen Cheng, Zhe Xu, Ming Du

“…The components of the bioactive casein hydrolysate were analyzed by nanoscale liquid chromatography quadrupole time-of-flight tandem mass spectrometry (NanoLC-Q-TOF-MS/MS). …”
Get full text

Systematic identification of the chemical components of leaves and roots of Didymocarpus heucherifolius Hand.-Mazz. based on UHPLC Q-Exactive Orbitrap MS technology coupled with mo... by Liangyin Shu, Shunli Xiao, Kailin Li, Huibin Luo, Meng Li, Qingrui Yang, Bowen Gao, Jiaxin Li, Fang Yan, Wei Cai

“…An efficient approach based on molecular networking strategy coupled with ultra high-performance liquid chromatography coupled with Q-Exactive Orbitrap tandem mass spectrometry (UHPLC Q-Exactive Orbitrap MS) was used to analyze the chemical components systematically. …”
Get full text

Assessment of Microbiological Indoor Air Quality in Cattle Breeding Farms by Justyna Szulc, Małgorzata Okrasa, Katarzyna Dybka-Stępień, Michael Sulyok, Adriana Nowak, Anna Otlewska, Bogumiła Szponar, Katarzyna Majchrzycka

“…Abstract This study, which was conducted on a farm in central Poland from February till April 2019, aimed to evaluate microbiological threats on cattle breeding premises by counting and identifying the microorganisms (via culturing and high-throughput sequencing), assessing the endotoxin concentrations (via gas chromatography-mass spectrometry [GC-MS]) and analysing the secondary metabolites (including mycotoxins) in the air and sedimented dust (via liquid chromatography-tandem mass spectrometry [LC-MS/MS]) in six barns. …”
Get full text

Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum by Yunliang Zheng, Nana Xu, Xingjiang Hu, Qiao Zhang, Yanpeng Liu, Qingwei Zhao

“…A simple, rapid, and sensitive liquid chromatography (LC)/mass spectrometry (MS) method was established and validated for simultaneous quantitation of pyrazinamide, isoniazid, rifampicin, and ethambutol in human blood sample. …”
Get full text

Anti-Neuroinflammatory Effects of Prenylated Indole Alkaloids from the Antarctic Fungus <i>Aspergillus</i> sp. Strain SF-7367 by Zhiming Liu, Chi-Su Yoon, Thao Quyen Cao, Hwan Lee, Il-Chan Kim, Joung Han Yim, Jae Hak Sohn, Dong-Sung Lee, Hyuncheol Oh

“…Secondary metabolites of SF-7367 were isolated using high-performance liquid chromatography followed by validation of their anti-inflammatory effects in lipopolysaccharide-stimulated BV2 microglia and RAW264.7 macrophages. …”
Get full text

Effect of Food on the Steady-State Pharmacokinetics of Tenofovir and Emtricitabine plus Efavirenz in Ugandan Adults by Mohammed Lamorde, Pauline Byakika-Kibwika, William S. Tamale, Francis Kiweewa, Mairin Ryan, Alieu Amara, John Tjia, David Back, Saye Khoo, Marta Boffito, Cissy Kityo, Concepta Merry

“…Drug concentrations in plasma were determined by liquid chromatography and tandem mass spectrometry. Geometric mean ratios (GMRs) and confidence intervals (CIs) of parameters were calculated (reference, fasting). …”
Get full text

The transport of polyphenols from Camellia fascicularis in Caco-2 cells based on UPLC-ESI-MS/MS by Shengjiang Duan, Hao Zheng, Junrong Tang, Huan Kan, Changwei Cao, Zhijiao Shi, Yun Liu

“…Herein, digestion samples (DS) of C. fascicularis polyphenols (CFPs) were metabolically analyzed based on their bidirectional translocation in Caco-2 cells using ultraperformance liquid chromatography-electrospray ionization-tandem mass spectrometry. …”
Get full text

Synthesis and Characterization of TiO2 Modified with Polystyrene and Poly(3-chloro-2-hydroxypropyl Methacrylate) as Adsorbents for the Solid Phase Extraction of Organophosphorus Pe... by Enrique Alejo-Molina, A. Rafael Vilchis-Néstor, David Muñoz-Rodríguez, Cristian Carrera-Figueiras

“…The pesticide recoveries obtained by high performance liquid chromatography ranged from 6 to 26% for TiO2-PClHPMA and 44 to 92% for TiO2-PS.…”
Get full text

Greener and rapid magnetic solid phase microextraction method using Fe3O4@Al2O3/AC for chromatographic determination of neonicotinoid pesticides in vegetable oils by Musiiwa V. Sengane, Masixole Sihlahla, Philiswa N. Nomngongo, Nomvano Mketo

“…A greener sample preparation method based on magnetic solid phase microextraction (MSPμE) was developed for the preconcentration of neonicotinoid pesticides (acetamiprid, imidacloprid, thiacloprid and thiamethoxam) in vegetable oil samples followed by separation and quantification using high performance liquid chromatography with diode array detector (HPLC-DAD). …”
Get full text

Two Cysteines in Raf Kinase Inhibitor Protein Make Differential Contributions to Structural Dynamics In Vitro by Hyun Sang Cho, Mohammad Faysal Al Mazid, Eun-Young Lee, Md Abu Rayhan, Hyoun Sook Kim, Byung Il Lee, Hye Jin You

“…Mutation of alanine or serine instead of cysteine in RKIP proteins did not affect the biochemical characteristics, while dynamic light scattering and liquid chromatography (LC) quadrupole time-of-flight (Q-TOF) mass spectrometry (MS) suggested distinct peaks in solution, which were identified via LC–MS/MS analyses, and further clarified the role of cysteine in RKIP dimerization. rhRKIP dimer formation was abrogated by a 32-aa peptide mimicking the region between two RKIP proteins for dimerization. …”
Get full text

Monitoring of immune activation using biochemical changes in a porcine model of cardiac arrest by Anton Amann, Bernhard Widner, Josef Rieder, Herwig Antretter, Georg Hoffmann, Viktoria Mayr, Hans-Ulrich Strohmenger, Dietmar Fuchs

“…Urinary neopterin concentrations were measured with high-performance liquid chromatography (HPLC) and radioimmunoassay (RIA) (BRAHMS Diagnostica, Berlin, Germany). …”
Get full text