Screening of six medicinal plant species for antileishmanial activity by Ferreira Fabiana Borges Padilha, Cabral Márcia Regina Pereira, Sarragiotto Maria Helena, Fernandez Carla Maria Mariano, Gazim Zilda Cristiani, Junior Ranulfo Piau, Demarchi Izabel Galhardo, Silveira Thaís Gomes Verzignassi, Lonardoni Maria Valdrinez Campana

“…After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. …”
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Fluralaner systemic treatment of chickens results in mortality in Triatoma gerstaeckeri, vector of the agent of Chagas disease by Cassandra Durden, Yuexun Tian, Koyle Knape, Cory Klemashevich, Keri N. Norman, John B. Carey, Sarah A. Hamer, Gabriel L. Hamer

“…Results Feeding on fluralaner-treated chickens resulted 50–100% mortality in T. gerstaeckeri over the first 14 days post-treatment but not later; in contrast, all insects that fed on fenbendazole- and ivermectin-treated chickens survived. Liquid chromatography tandem mass spectrometry (LC-QQQ) analysis, used to detect the concentration of fluralaner and fenbendazole in chicken plasma, revealed the presence of fluralaner in plasma at 3, 7, and 14 days post-treatment but not later, with the highest concentrations found at 3 and 7 days post-treatment. …”
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Age effects on measurements of hypothalamic-pituitary-adrenal axis activation in laying hens by Meagan E. Abraham, Priscila Serpa, Gregory S. Fraley, Darrin M. Karcher

“…From these blood samples, white blood cell (WBC) differential counts, heterophil:lymphocyte (H:L) ratios (N = 10/treatment), and corticosterone and cortisol levels were measured using a high-performance liquid chromatography (HPLC) system coupled to a mass spectrometer (MS/MS) (N = 5/treatment). …”
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Effects of supplemental feeding of feeds with different protein levels on the metabolism of the gut microbiota of bighead carp (Hypophthalmichthys nobilis) by Zheng Zhang, Junru Wang, Xiaomu Yu, Xiaoyu Zhou, Yanhong Wu, Jun Zou, Jingou Tong

“…We used 16S ribosomal ribonucleic acid sequencing and liquid chromatography-mass spectrometry (LC-MS) techniques to study three groups of bighead carp. …”
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Mid-infrared spectra of dried and roasted cocoa (Theobroma cacao L.): A dataset for machine learning-based classification of cocoa varieties and prediction of theobromine and caffe... by Gentil A. Collazos-Escobar, Andrés F. Bahamón-Monje, Nelson Gutiérrez-Guzmán

“…Infrared data were acquired using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy, while High-Performance Liquid Chromatography (HPLC) was employed to accurately quantify theobromine and caffeine in the dried cocoa beans. …”
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Quantitative proteomic studies of the intestinal mucosa provide new insights into the molecular mechanism of ulcerative colitis by Yandong Guo, Dahal Pabitra, Lei Pan, Lanbo Gong, Aimin Li, Side Liu, Jing Xiong

“…Methods Intestinal mucosal biopsy tissue specimens of inflamed (ulcerative colitis-inflamed, UC-I) and non-inflamed (ulcerative colitis-noninflamed, UC-N) tissue were obtained simultaneously during colonoscopy from newly diagnosed UC patients prior to any treatments. Label-free liquid chromatography tandem mass spectrometry (LC-MS/MS) quantitative proteomics was used to detect proteomic differences between UC-I, UC-N, and normal control subjects (n = 5). …”
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Assessment of healthcare workers’ knowledge and availability of resources for sickle cell disease management in Bukavu, Democratic Republic of the Congo by Nash Mwanza Nangunia, Olivier Mukuku, Viviane Bianga Feza, Yves Mulindilwa Kyembwa, Théophile Barhwamire Kabesha, André Kabamba Mutombo, Stanislas Okitostho Wembonyama

“…Regarding resources, 55.2% of HCFs had a falciform test, but none had advanced technologies such as isoelectrofocusing or high-performance liquid chromatography. Conclusion This study reveals a general lack of knowledge among HCWs about SCD in Bukavu, as well as limited availability of resources for diagnosis and treatment. …”
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Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies by El-Mernissi Rafik, El Menyiy Naoual, Metouekel Amira, Zouhri Aziz, El-Mernissi Yahya, Siddique Farhan, Nadeem Sumaira, Amhamdi Hassan, Abboussi Oualid, Alsahli Abdulaziz Abdullah, Bourhia Mohammed, Dauelbait Musaab, Shazly Gamal A., Hajji Lhoussain

“…Ultra-high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry analysis revealed a rich phenolic composition, including benzoic acid, cannabisin B, genistein, and epicatechin. …”
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Targeted Plasma Bile Acid Metabolomic Analysis in Metabolic Dysfunction-Associated Steatohepatitis and Alcoholic Hepatitis by Yuta Hirata, Yasunaru Sakuma, Hideo Ogiso, Ryozo Nagai, Kenichi Aizawa

“…Plasma samples from patients with MASH, AH, and a control group were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to quantify bile acid concentrations. …”
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Danshen-Honghua Ameliorates Stress-Induced Menopausal Depression in Rats by Simeng Gu, Yao Ma, Kemin Ge, Ruifang Nie, Erxi Wu, Yang Li

“…Also, the levels of norepinephrine (NE), dopamine (DA), serotonin (5-HT), and cortisone were determined by high-performance liquid chromatography (HPLC). Serum estradiol (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were determined by radioimmunoassay. …”
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Effect of French Press Brewing on the Sensory Quality of Robusta Coffee Produced in Hainan by LIU Di, LAO Fei, DONG Wenjiang, YU Xinxin

“…Single factor experiments and response surface methodology were used jointly for optimization of four processing parameters based on consumer preference, and the correlation between consumer preference and sensory characteristics and flavor components was analyzed by quantitative sensory description, gas chromatography-mass spectrometry (GC-MS), and ultra performance liquid chromatography (UPLC). The results showed that the extraction yield of coffee significantly increased with the decrease in coffee grind size, the increase in powder-to-water ratio, the increase in brewing water temperature or the prolongation of brewing time (P < 0.05). …”
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Recovery of Bioactive Constituents from Olive Leaf Pruning Waste of Five Different Cultivars: A Comparison of Green Extraction Techniques to Maximize Health Benefits by Hamid Mushtaq, Simona Piccolella, Jose A. Mendiola, Lidia Montero, Elena Ibáñez, Severina Pacifico

“…Chemical profiling by UHPLC-HR-MS/MS (ultra-high-performance liquid chromatography high-resolution tandem mass spectrometry) and GC-MS (gas chromatography mass spectrometry) showed that UAM-EtOH effectively extracted polyphenols (especially luteolin derivatives) and triterpenes (notably maslinic acid), while PLE yielded the highest amount of secoiridoids (e.g., secologanoside). …”
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The potential of Sonneratia caseolaris mangrove leaves extract as a bioactive food ingredient using various water extract [version 4; peer review: 2 approved] by Maharani Pertiwi Koentjoro, Bambang Semedi, Ike Listya Anggraeni, Hartati Kartikaningsih, Nur Fitriana

“…Methods Analysis of total flavonoids, total phenols, identification of active compounds with Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS), and bioinformatics were also carried out to obtain temporary conclusions about the antioxidant activity of S. caseolaris leaf extract. …”
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Hemoglobin-Derived Antioxidant Peptides from Beef Cattle in Ningxia: Identification Based on Computerized Screening and Molecular Docking by XU Feiran, WANG Yu, WANG Xiaojing, CUI Wei, WANG Yongkun, ZHOU Hui, WANG Zhaoming, CAI Kezhou, ZHANG Bao, XU Baocai

“…Amino acid analysis and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to identify the amino acid compositions and sequences of the hydrolysates with molecular mass < 3 kDa. …”
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In vitro neuroprotective potentials of aqueous and methanol extracts from Heinsia crinita leaves by Ganiyu Oboh, Esther E. Nwanna, Sunday I. Oyeleye, Tosin A. Olasehinde, Opeyemi B. Ogunsuyi, Aline A. Boligon

“…The total phenol and flavonoid contents of the extracts were determined using colorimetric method while phenolic characterization of the leaf was analyzed via high performance liquid chromatography-diode array detector (HPLC-DAD). The effects of the extracts on Fe2+-induced lipid peroxidation in rats’ brain homogenate, monoamine oxidase (MAO), Na+/K+-ATPase, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities were also assessed. …”
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Active Compounds of Red Ginger as Antioxidant Activity in the Supplementation and Treatment of Depression by Ira Aini Dania, Rusdiana Rusdiana, Aldy S. Rambe, Urip Harahap, Elmeida Effendy, Nanda Novziransyah

“…This study was experimental analytical study for analyzing secondary metabolism using phytochemical screening tests and examining active compounds with the combination of liquid chromatography with tandem mass spectrometry (LCMS/MS).Red ginger extracts contain secondary metabolic flavonoids of 48.79 mgQE/g, phenol 92.73 mgGAE/G and antioxidants of 39.96 ppm. …”
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Action Mechanisms of Metformin Combined with Exenatide and Metformin Only in the Treatment of PCOS in Obese Patients by Jingwen Gan, Jie Chen, Rui-lin Ma, Yan Deng, Xue-song Ding, Shi-yang Zhu, Ai-jun Sun

“…Glucagon-like peptide (GLP)-1 receptor agonists (GLP-1Ra), such as exenatide, can bind to specific receptors on tissues such as the ovaries to improve the clinical phenotype of PCOS, while insulin-sensitizing agents, such as metformin, can also benefit to metabolic abnormalities in PCOS. Liquid chromatography-mass spectrometry (LC/MS) metabolomics revealed differences between the mechanisms of exenatide and metformin treatment of PCOS to some extent. …”
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Difference in the Vitreal Protein Profiles of Patients with Proliferative Diabetic Retinopathy with and without Intravitreal Conbercept Injection by Chen Zou, Minjie Zhao, Jingjing Yu, Dandan Zhu, Yunzhi Wang, Xinping She, Yanan Hu, Zhi Zheng

“…To examine the difference in the vitreal protein profiles of patients with proliferative diabetic retinopathy (PDR) with and without preoperative intravitreal conbercept (IVC) treatment. Methods. Liquid chromatography-tandem mass spectrometry- (LC-MS/MS-) based proteomic methods were used to determine the protein profiles of the vitreous humor in patients with PDR treated with (IVC group; n=9) and without (PDR group; n=8) preoperative IVC. …”
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Phenolic Profile and Antioxidant Activity of Crude Extracts from Microalgae and Cyanobacteria Strains by Idaira Jerez-Martel, Sara García-Poza, Gara Rodríguez-Martel, Milagros Rico, Cristina Afonso-Olivares, Juan Luis Gómez-Pinchetti

“…In addition, the identification of 4 simple phenolics (gallic, syringic, protocatechuic, and chlorogenic acids) and the flavonoids (+) catechin and (-) epicatechin was carried out by using reverse phase high performance liquid chromatography. The strain Euglena cantabrica showed the highest concentration of phenolic compounds, particularly gallic and protocatechuic acids (5.87 and 2.97 mg per gram of dried biomass, resp.). …”
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Indoor Air Exposure to Multiple Agricultural Pesticides Potentially Posing the Highest Risk to Young Children by Sithembiso Sifiso Msibi, Chung-Yu Chen, Cheng-Ping Chang, Chiou-Jong Chen, Su-Yin Chiang, Kuen-Yuh Wu

“…This case study assessed such exposure and its risks by sampling and analyzing the air inside the homes of 15 pesticide applicators and 12 non-applicators in Swazi communities surrounded by nearby (~200 m) sugarcane fields on pesticide spraying days. Applying liquid chromatography with tandem mass spectrometry (LC-MS/MS) to the samples revealed mean ametryn, atrazine, pendimethalin and 2,4-dichlorophenoxyacetic acid (2,4-D) concentrations of 0.75, 0.32, 0.57 and 0.004 µg m−3, respectively, in the applicator households and corresponding values of 0.19, 0.03, 0.04 and 0.003 µg m−3, respectively, in the non-applicator households. …”
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