Deciphering cellular complexity: advances and future directions in single-cell protein analysis by Qirui Zhao, Qirui Zhao, Qirui Zhao, Shan Li, Shan Li, Shan Li, Leonard Krall, Leonard Krall, Leonard Krall, Qianyu Li, Rongyuan Sun, Yuqi Yin, Jingyi Fu, Xu Zhang, Xu Zhang, Xu Zhang, Yonghua Wang, Yonghua Wang, Yonghua Wang, Mei Yang, Mei Yang, Mei Yang

“…This review provides a comprehensive examination of the latest methodologies, including sophisticated cell isolation techniques (Fluorescence-Activated Cell Sorting (FACS), Magnetic-Activated Cell Sorting (MACS), Laser Capture Microdissection (LCM), manual cell picking, and microfluidics) and advanced approaches for protein profiling and protein-protein interaction analysis. …”
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CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms by Tinatini Tchatchiashvili, Tinatini Tchatchiashvili, Mateusz Jundzill, Mateusz Jundzill, Mike Marquet, Kamran A. Mirza, Kamran A. Mirza, Mathias W. Pletz, Mathias W. Pletz, Oliwia Makarewicz, Oliwia Makarewicz, Lara Thieme, Lara Thieme

“…Biofilm viability was assessed using CAM/TMA-DPH or SYTO9/PIstaining, followed by analysis with confocal laser scanning microscopy (CLSM) and ImageJ-based biofilm surface coverage quantification. …”
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Clinical Evaluation of LASEK for High Myopia Correction between the Triple-A Profile and the Zyoptix Tissue Saving Profile by Min Li, Yuehui Shi, Lina Sun, Lin Liu, Chen Qu, Jun Zou

“…To compare the effects of correcting high myopia using the MEL®90 Triple-A profile LASEK at a 500 Hz pulse rate (Triple-A group) versus the Zyoptix tissue-saving ablations of Technolas 217z laser platform at 100 Hz (TS group). Methods. This retrospective study included 50 eyes in the Triple-A group and 42 eyes in the TS group with manifest refraction spherical equivalent (MRSE) of −6 diopters (D) to −10 D. …”
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Cross-sectional imaging of speed-of-sound distribution using photoacoustic reversal beacons by Yang Wang, Danni Wang, Liting Zhong, Yi Zhou, Qing Wang, Wufan Chen, Li Qi

“…Herein, we propose a method for SOS imaging using scanned photoacoustic beacons excited by short laser pulse with inversion reconstruction. Our method is based on photoacoustic reversal beacons (PRBs), which are small light-absorbing targets with strong photoacoustic contrast. …”
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In vitro evaluation of hypochlorous acid-silver nanoparticle waterline disinfectant for dental unit waterline disinfection by Tingting Yin, Qiaowen Li, Huan Sun, Jin Zheng, Yuanyuan Wang, Yi Luo, Li Wang

“…Results The growth of biofilm on the waterlines was observed using scanning electron microscopy (SEM) and laser confocal microscopy (LCM). The results indicated that AgNP solution was successfully prepared and demonstrated excellent stability. …”
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Ginger-Derived Exosome-Like Nanoparticles Loaded With Indocyanine Green Enhances Phototherapy Efficacy for Breast Cancer by Guo Z, Li G, Shen L, Pan J, Dou D, Gong Y, Shi W, Sun Y, Zhang Y, Ma K, Cui C, Li W, Liu Q, Zhu X

“…When irradiated by an 808 nm NIR laser, GDNPs@ICG generated high levels of ROS, MDA, and local hyperthermia within the tumor, which caused lipid peroxidation and ER stress, thus enhancing the photo-mediated breast tumor therapy effect. …”
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Effect of Extracorporeal Shock Wave Therapy Combined with Kinesio Taping on Plantar Fasciitis by Mingliang JIN, Hua YUAN, Xu HU, Xu JIN, Hongru YUAN, Jiajin FAN

“…The control group received routine rehabilitation treatment, including low-power He-Ne laser, ultrasonic therapy, exercise therapy (triceps of the leg, plantar fascia relaxation). …”
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In Situ Growth of (−201) Fiber‐Textured β‐Ga2O3 Semiconductor Tape for Flexible Thin‐Film Transistor by Xiao Tang, Yue Zhao, Kuang‐Hui Li, Chen Liu, Hendrik Faber, Wedyan Babatain, Che‐Hao Liao, Saravanan Yuvaraja, Vishal Khandelwal, Dhanu Chettri, Haicheng Cao, Yi Lu, Chuanju Wang, Thomas D. Anthopoulos, Xixiang Zhang, Xiaohang Li

“…In the research, for the first time, Ga2O3 thin films are directly grown on SiOx/Al2Ox buffered Hastelloy substrates. With pulsed laser deposition under high temperature, as‐grown Ga2O3 thin films have a fabric texture with a preferred out‐of‐plane orientation along (−201) and a good field‐effect mobility of 18.07 cm2 V−1 s−1. …”
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Knowledge, Attitude, and Practice (KAP) Survey toward Skin Cancer among Ecuadorian Population by Victor H. Pinos-León, César Sandoval, Franklin Cabrera, Esmeraldas Terán, Ana Garnica, Ana Kellendonk, Mery Alvear, Carla Rosero, Lorena Vaca, Jorge Bonifaz, Anita Buestán, Claudia Armas, Raquel Trujillo, Patricio Freire, Tamara León, Giselle Erazo, Liliana García, Marcela Alzate, Vicente Toapanta, Myriam Ortega, Daniela Caicedo, Alicia Pereira, Lenin Gómez-Barreno, Juan S. Izquierdo-Condoy, Merry Charlie, Esteban Ortiz-Prado, Katherine Simbaña-Rivera

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Role of Intravitreal Antivascular Endothelial Growth Factor Injections for Choroidal Neovascularization due to Choroidal Osteoma by Ahmad M. Mansour, J. Fernando Arevalo, Eman Al Kahtani, Hernando Zegarra, Emad Abboud, Rajiv Anand, Hamid Ahmadieh, Robert A. Sisk, Salman Mirza, Samuray Tuncer, Amparo Navea Tejerina, Jorge Mataix, Francisco J. Ascaso, Jose S. Pulido, Rainer Guthoff, Winfried Goebel, Young Jung Roh, Alay S. Banker, Ronald C. Gentile, Isabel Alonso Martinez, Rodney Morris, Neeraj Panday, Park Jung Min, Emilie Mercé, Timothy Y. Y. Lai, Vicky Massoud, Nicola G. Ghazi

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Photodynamic Therapy for Oral Squamous Cell Carcinoma: A Systematic Review and Meta-Analysis by Jiao Lin, Guangcheng Ni, Tingting Ding, Shangxue Lei, Liang Zhong, Na Liu, Keran Pan, Ting Chen, Xin Zeng, Hao Xu, Taiwen Li, Hongxia Dan

“…A subgroup analysis of lesion site, photosensitizer, laser type, radiant exposure, and power density revealed no statistically significant differences. …”
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The spatially informed mFISHseq assay resolves biomarker discordance and predicts treatment response in breast cancer by Evan D. Paul, Barbora Huraiová, Natália Valková, Natalia Matyasovska, Daniela Gábrišová, Soňa Gubová, Helena Ignačáková, Tomáš Ondris, Michal Gala, Liliane Barroso, Silvia Bendíková, Jarmila Bíla, Katarína Buranovská, Diana Drobná, Zuzana Krchňáková, Maryna Kryvokhyzha, Daniel Lovíšek, Viktoriia Mamoilyk, Veronika Mancikova, Nina Vojtaššáková, Michaela Ristová, Iñaki Comino-Méndez, Igor Andrašina, Pavel Morozov, Thomas Tuschl, Fresia Pareja, Jakob N. Kather, Pavol Čekan

“…On a retrospective cohort of 1082 female breast tissues, we develop and validate mFISHseq, which integrates multiplexed RNA fluorescent in situ hybridization with RNA-sequencing, guided by laser capture microdissection. This technique ensures tumor purity, unbiased whole transcriptome profiling, and explicitly quantifies intratumoral heterogeneity. …”
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Canopy structural heterogeneity drives α and β species–genetic diversity correlations in a Chinese subtropical forest by Zhiliang Yao, Xia Pan, Xin Yang, Xiaona Shao, Bin Wang, Yun Deng, Zhiming Zhang, Qiaoming Li, Luxiang Lin

“…We also quantified microhabitat variation by quantifying canopy structure heterogeneity with airborne laser scanning on 20 1-ha subtropical forest plots. …”
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Triceps insertion violation from commonly applied olecranon plating system: a comparison by Ahmed A. Habis, MD, MSc, FRCSC, Kevin Nguyen, MASc, Julie Chan, MD, MHSc, FRCSC, Emad Anam, MD, MSc, FRCSC, Ryan T. Bicknell, MD, MSc, FRCSC, Heidi-Lynn Ploeg, PhD, PEng, FASME, FORS, Parham Daneshvar, MD, FRCSC

“…Olecranon plates, triceps insertion footprints, and olecranon surface areas were digitized as 3D surface models with a laser scanner (SG100; ShapeGrabber Inc., Ottawa, Canada). …”
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Preparation and in vitro biological evaluation of strontium doped β-tricalcium phosphate scaffolds for alveolar bone tissue regeneration by Congrui Liu, Guiyong Xiao, Zichen Wang, Mengchen Xu, Yinchuan Wang, Nan Wang, Zhenlong Sun, Xiaoyan Li, Yixin Yin

“…The density enhanced with the increasing amount of Sr, achieving a compressive strength of 3.86 MPa in the Sr8-TCP scaffold, meeting the criteria for cancellous bone implantation. Confocal laser scanning microscopy analysis revealed that Srx-TCP offered favorable circumstances for cell adhesion. …”
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Overcoming hypoxia-induced breast cancer drug resistance: a novel strategy using hollow gold-platinum bimetallic nanoshells by Lian-Ying Zhang, Xiao-Tong Chen, Rong-Tian Li, Wei Meng, Guo-Qin Huang, Yong-Jian Chen, Feng-Jun Ge, Qun Zhang, Yu-Jun Quan, Cai-Tao Zhang, Yi-Fei Liu, Ming Chen, Jin-Xiang Chen

“…The photothermal effect, induced by the surface plasmon resonance (SPR) from the Au-Pt shell under near infrared-II (NIR-II) laser irradiation, not only directly causes tumor cell death through photothermal therapy (PTT), but also significantly enhances the catalase-like activity between Pt nanoparticles and endogenous H2O2. …”
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Single-cell atlas reveals multi-faced responses of losartan on tubular mitochondria in diabetic kidney disease by Zhen Zhu, Guangxin Luan, Song Wu, Yiyi Song, Shuang Shen, Kaiyue Wu, Shengnan Qian, Weiping Jia, Jun Yin, Tao Ren, Jianping Ye, Li Wei

“…Preliminary validation through in vitro and in vivo experiments, including observation of changes in mitochondrial morphology and dynamics using probes such as Mitotracker Red, and evaluation of the effect of losartan on key events of mitochondrial homeostasis perturbation using electron microscopy, laser confocal microscopy, immunofluorescence, and Western blotting. …”
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The transcription factor combination MEF2 and KLF7 promotes axonal sprouting in the injured spinal cord with functional improvement and regeneration-associated gene expression by Callan L. Attwell, Inés Maldonado-Lasunción, Ruben Eggers, Bastiaan A. Bijleveld, Ward M. Ellenbroek, Natascha Siersema, Lotte Razenberg, Dédé Lamme, Nitish D. Fagoe, Ronald E. van Kesteren, August B. Smit, Joost Verhaagen, Matthew R. J. Mason

“…To determine whether they could activate the RAG program we carried out gene expression profiling on laser-dissected dorsal root ganglion neurons specifically expressing these TF combinations, and of DRG neurons that had been axotomized. …”
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Curcumin-enhanced NIR-II-responsive gold nanobipyramids for targeted HSP 90 inhibition by Zhenying Diao, Youcheng Liang, Yong Liu, Dou Zhang, Long Qiu, Jianbo Sun, Qiaoyou Lu, Yanlei Liu, Daxiang Cui, Ting Yin

“…Under the irradiation of NIR-II light, the obtained GNBs-Cur blocked the expression of HPS90 and inhibited related antiapoptotic pathways, thus enhancing the mild PTT of GNBs under 1064 nm laser irradiation. Meanwhile, Cur served as chemotherapeutic agents to induce apoptosis in tumor cells. …”
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Prevailing Antibiotic Resistance Patterns in Hospitalized Patients with Urinary Tract Infections in a Vietnamese Teaching Hospital (2014 – 2021) by Le HHL, Thuc LC, Ta TB, Tran TV, Hung DV, Kien HT, Le MN, Luong VH, Nguyen VTH, Pham HQ, Le HV, Viet NH, Hoang LH, Nguyen TT, Latsavong M, Le TD, Trong Tuan D, An NV

“…Hai Ha Long Le,1,2 Luong Cong Thuc,3 Thang Ba Ta,4 Tien Viet Tran,5 Dinh Viet Hung,6 Hoang Trung Kien,7 Minh Nhat Le,8,9 Vu Huy Luong,10,11 Vinh Thi Ha Nguyen,11,12 Hoa Quynh Pham,13 Hung Van Le,11,13 Nguyen Hoang Viet,14 Le Huy Hoang,15 Tram Thuy Nguyen,15 Mixay Latsavong,16 Tuan Dinh Le,17 Dao Trong Tuan,18 Nguyen Van An19 1Department of Clinical Microbiology and Parasitology, Faculty of Medical Technology, Hanoi Medical University, Hanoi, Vietnam; 2Department of Biochemistry, Hematology and Immunology, National Hospital of Dermatology and Venereology, Hanoi, Vietnam; 3Cardiovascular Center, Military Hospital 103, Vietnam Military Medical University, Hanoi, Vietnam; 4Respiratory Center, Military Hospital 103, Vietnam Military Medical University, Hanoi, Vietnam; 5Department of Infectious Diseases, Military Hospital 103, Vietnam Military Medical University, Hanoi, Vietnam; 6Department of Psychiatry, Military Hospital 103, Vietnam Military Medical University, Hanoi, Vietnam; 7Department of Immunology, Vietnam Military Medical University, Hanoi, Vietnam; 8Tay Nguyen Institute of Science Research, Vietnam Academy of Science and Technology, VAST, Hanoi, Vietnam; 9Antimicrobial Resistance Research Center, National Institute of Infectious Disease, Tokyo, Japan; 10Department of Laser and Skincare, National Hospital of Dermatology and Venereology, Hanoi, Vietnam; 11Department of Dermatology and Venereology, Hanoi Medical University, Hanoi, Vietnam; 12Department of General Planning, National Hospital of Dermatology and Venereology, Hanoi, Vietnam; 13Department of Microbiology, Mycology and Parasitology, National Hospital of Dermatology and Venereology, Hanoi, Vietnam; 14Molecular Pathology Department, Faculty of Medical Technology, Hanoi Medical University, Hanoi, Vietnam; 15Department of Bacteriology, National of Hygiene and Epidemiology, Hanoi, Vietnam; 16Department of Nephrology-Hemodialysis, Military Central Hospital 103, Vientiane, Laos; 17Department of Rheumatology and Endocrinology, Military Hospital 103, Vietnam Military Medical University, Hanoi, Vietnam; 18ENT Department, Central Military Hospital 108, Hanoi, Vietnam; 19Department of Microbiology, Military Hospital 103, Vietnam Military Medical University, Hanoi, VietnamCorrespondence: Nguyen Van An, Department of Microbiology, Military Hospital 103, Vietnam Military Medical University, Hanoi, 100000, Vietnam, Tel + 84 982 860 055, Email ank59hvqy@gmail.comPurpose: In a Vietnamese teaching hospital, this study examined the prevalence and patterns of antimicrobial resistance (AMR) of common bacteria isolated from hospitalized patients with urinary tract infections (UTIs) between 2014 and 2021.Methods: From 4060 urine samples collected, common pathogens were isolated using quantitative culture on brilliance UTI Clarity agar and blood agar. …”
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