Exploring the Analgesic Effect of Acupuncture on Knee Osteoarthritis Based on MLT/cAMP/PKA/CREB Signaling Pathway by Zhang C, Yu M, Zhang L, Zhou X, Han J, Fu B, Xue H, Zhang C

“…In addition, acupuncture significantly reduced the expression of the inflammatory factor MMP-3 in knee joint fluid and significantly increased the levels of MLT, AANAT, MT1, MT2, cAMP, PKA and CREB.Conclusion: By regulating sympathetic excitability, acupuncture may activate the MLT/cAMP/PKA/CREB signaling pathway, decrease inflammatory factor expression and slow down degradation of articular cartilage, resulting in the relief of knee pain.Keywords: acupuncture, knee osteoarthritis, MLT/cAMP/PKA/CREB pathway, melatonin…”
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Ten days of supplementation with a standardized Boswellia serrata extract attenuates soreness and accelerates recovery after repeated bouts of downhill running in recreationally ac... by Dawna Salter, Himana Yalamanchi, Aiswarya Yalamanchi, Amulya Yalamanchi

“…The SBS-supplemented subjects reported less knee joint pain after DHR than the PLA group (p < 0.05 on days 8, 9, and 10). …”
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Arctiin Protects Chondrocytes from Interleukin-1β-Induced Inflammation and Apoptosis by RNA Sequence In vivo and In vitro by Yong-Jia Song, Jia-Min Bao, Zeng-Qiao Zhang, Yun-Xiang Hai, Hao-Nan Wen, Tian-Jun Zhai, Wei Feng, Min Song

“…Furthermore, Safranin-O fast green staining and immunohistochemistry staining were performed on slices of the mice knee joint to evaluate the OA Research Society International score, alterations in the cartilage matrix, and levels of apoptosis-related proteins at sites of knee cartilage damage in an arthritis model induced by monosodium iodoacetate (MIA) and physical activity. …”
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Hubungan Indeks Massa Tubuh dengan Derajat Kerusakan Sendi pada Pasien Osteoartritis Lutut di RSUP Dr. M. Djamil Padang by Endang Mutiwara, Najirman ., Afriwardi .

“…The radiograph of knee osteoarthritis shows changes of knee joint which assessed based on Kellgren and Lawrence criteria. …”
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Dual-energy CT-derived virtual noncalcium imaging to assess bone marrow lesions in patients with knee osteoarthritis by Wei Chen, Limin Liu, Heng Zhao, Hui Li, Jing Luo, Yao-lin Qu, Dan Zhang, Ya-han He, Yi-sha Pan, Fang Gao, Hua-zhi Liao, Xiao-long Chen, Hao Lei, De-qiu Tang, Fei Peng

“…VNCa images and MRI were independently scored by three readers using a four-level scoring system over 15 anatomical subregions in each knee joint. Spearman correlation coefficient was used for total BML scores on DECT and MRI correlation with WOMAC and KOOS. …”
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A ROS-responsive hydrogel encapsulated with matrix metalloproteinase-13 siRNA nanocarriers to attenuate osteoarthritis progression by Qiuyang Wang, Kai Feng, Guangsheng Wan, Wei Liao, Jing Jin, Peng Wang, Xiaolian Sun, Weijun Wang, Qing Jiang

“…Studies in vitro demonstrated that si-Fe-HPP exhibited excellent biocompatibility, anti-inflammatory effects and prolonged stable retention time in knee joint. Intra-articular injection of si-Fe-HPP significantly attenuated cartilage degradation in mice with destabilization of the medial meniscus (DMM)-induced OA. …”
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The effects of contralateral limb cross-education training on post-surgical rehabilitation outcomes in patients with anterior cruciate ligament reconstruction: a randomized control... by Chao Liu, ShiJia Li, JianPing Li, HongHao Zhang, GuQiang Li, XiangZhan Jiang

“…Abstract Objective This study examines whether cross-education training of the healthy limb promotes cross-transfer through central nervous system stimulation, enhancing the function, kinematic parameters, dynamic balance, and plantar pressure of the affected knee joint in patients recovering from postoperative anterior cruciate ligament reconstruction (ACLR). …”
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Hydrogen-oxygen mixture inhalation as an adjunctive treatment to home-based exercise in older patients with knee osteoarthritis: an open-label, blinded-endpoint, randomized control... by Chenhui Wang, Mengwei Yan, Yuru Li, Lei Han, Hongqian Wang, Shufeng Jia, Xingchen Liu, Yang Liu, Fan Wu, Baoguo Wang

“…Both groups participated in a 12-week home-based exercise (HBE) program, which included knee-joint exercises and health education. Group H additionally received H2-O2 inhalation for 60 min per day over 2 weeks, while Group C did not. …”
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Joint fluid multi-omics improves diagnostic confidence during evaluation of children with presumed septic arthritis by Yolanda Peña-López, Naureen G. Tareen, Bo Zhang, Indu Raman, Carlos A. Arana, Chengsong Zhu, Yang Liu, Pratibha Selvakumar, Nicolai S. C. van Oers, Simrat Morris, Lora V. Hooper, Lawson A. B. Copley, Prithvi Raj

“…The multi-omics combined bacterial 16S rRNA metagenomics, single cell transcriptomics, and proteomics on knee joint fluid specimens. The diagnostic value of the multi-omics was ascertained relative to standard of care culture and PCR-negative results. …”
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Impact of intermediate-dose tertiary prophylaxis on quality of life and psychological aspects of adult patients with severe/moderate hemophilia A by Hua Gao, Jia Liu, Shiqiu Zhou, Jing Gao, Jing Tan, Rong Chen

“…Significant correlations were observed for HAEMO-QoL-A with functional independence (P < 0.001), mood and emotions with age (P = 0.032), PHQ-9 scores with knee joint rehabilitation scores (P = 0.047), and GAD-7 scores with treatment experience and ankle joint rehabilitation scores (P = 0.029, P = 0.039).Conclusion Intermediate-dose tertiary prophylaxis with rhFVIII can improve quality of life but not relieve anxiety and depression in adults with severe/moderate hemophilia A. …”
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Comparison of Therapeutic Effects of Different Rehabilitation Methods on Patients Undergoing Total Knee Arthroplasty: A Network Meta‐Analysis of Randomized Controlled Trials by Zhaokai Jin, Yi Tang, Hua Huang, Lei Chen, Zhongyi Zhang, Tianyou Ma, Zhengming Wang, Hai Su, Haojing Zhou, Shuaijie Lv, Peijian Tong

“…A Bayesian network meta‐analysis (NMA) was carried out for calculating standardized mean difference (SMD) and the surface under cumulative ranking curve (SUCRA) of improvement of knee joint function after TKA by different rehabilitation methods. …”
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Mechanism of Inhibition of Inflammation in Osteoarthritis by Tougu Xiaotong Capsule via Regulating Ras-Raf-MEK1/2-ERK1/2 Signaling Pathway by Munan LIN, Xiang SHAO, Limei XU, Xin ZHANG, Zaishi ZHU, Yidan HAN, Xinwei DUAN, Jianmei CHEN, song GAO, Xihai LI

“…Isolated chondrocytes of passage two were divided into blank group, model group(treatment with 10 ng/mL of LPS for 8 h), inhibitor group(treatment with 10 ng/mL of U0126 for 30 min followed by 10 ng/mL of LPS for additional 8 h)and TXC group(treatment with 10 ng/mL of LPS plus 300 μg/mL of TXC for 8 h).Western blot was carried out to detect the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3 and MMP-13.qPCR was used to detect the mRNAs expression of MEK1/2, ERK1/2, MMP-3 and MMP-13, as well as the expression of miR-27b, miR-34a and miR-146a.Results:1)The cytoplasm of isolated cells(P2)from the cartilage of knee joint of 4-week-old SD male rats was stained brown by typeⅡcollagen immunohistochemical staining as compared with the negative control cells, which indicated that the extracted cells were chondrocytes.2)The expression of Ras, Raf, MEK1/2, MMP-3, MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of p-ERK1/2 had no significant difference(<italic>P</italic>&gt;0.05);the expression of Ras, Raf, p-ERK1/2, MEK1/2, MMP-3, MMP-13 in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05), but the expression of MEK1/2 in the TXC group had no significant difference compared to that in the model group(<italic>P</italic>&gt;0.05).3)The mRNA expression of MEK1/2, ERK1/2, MMP-3 and MMP-13 in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01);their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).4)The expression of miR-27b in the model group was significantly lower than that in the blank group(<italic>P</italic>&lt;0.01), but its expre-ssion in both the inhibitor group and the TXC group was higher than that in the model group(<italic>P</italic>&lt;0.01);the expression of both miR-34a and miR-146a in the model group was significantly higher than that in the blank group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05);however, their expression in both the inhibitor group and the TXC group was lower than that in the model group, respectively(<italic>P</italic>&lt;0.01, <italic>P</italic>&lt;0.05).Conclusion:Tougu Xiaotong capsule regulates the expression of key factors in Ras-Raf-MEK1/2-ERK1/2 signaling pathway and miR-27b, miR-34a and miR-146a to regulate the inflammatory of chondrocytes, thereby inhibiting osteoarthritis inflammation and protecting articular cartilage.…”
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Structurally sophisticated 3D-printed PCL-fibrin hydrogel meniscal scaffold promotes in situ regeneration in the rabbit knee meniscus by Hebin Ma, Bowen Xie, Hongguang Chen, Lifang Hao, Haigang Jia, Dengjie Yu, Yuanbo Zhou, Puzhen Song, Yajing Li, Jing Liu, Kaitao Yu, Yantao Zhao, Yadong Zhang

“…A meniscus injury is a common cartilage disease of the knee joint. Despite the availability of various methods for the treatment of meniscal injuries, the poor regenerative capacity of the meniscus often necessitates resection, leading to the accelerated progression of osteoarthritis. …”
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Identification of WDR74 and TNFRSF12A as biomarkers for early osteoarthritis using machine learning and immunohistochemistry by Yiwei Chen, Yiwei Chen, Jiali Lin, Detong Shi, Yu Miao, Yu Miao, Feng Xue, Feng Xue, Kexin Liu, Kexin Liu, Xiaotao Wang, Changqing Zhang, Changqing Zhang

“…The IHC validation on human knee joint specimens confirmed the upregulation of WDR74 and TNFRSF12A in OA tissues, corroborating their potential as diagnostic biomarkers.ConclusionsWDR74 and TNFRSF12A as principal biomarkers highlighted their attractiveness as therapeutic targets. …”
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Mechanism of Delaying Cartilage Degeneration in Knee Osteoarthritis with Rongjin Niantong Recipe Based on lncRNA NEAT1 and Nrf2/ARE Pathway by FU Changlong, XIE Xinyu, QIU Zhiwei, HUANG Yanfeng, JIN Linglu, LI Xihai

“…Under anesthesia, 0.05 mL sodium iodoacetate at a concentration of 20 mg/mL was injected into the right knee joint to establish the knee osteoarthritis model, and the blank group was injected with the same amount of normal saline. …”
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Effect of Multi-Joint Functional Electrical Stimulation Combined with Conventional Rehabilitation Therapy on Lower Limb Motor Function of Patients with Stroke by LIU Jiapeng, WANG Weining, XU Dongyan, WANG Nianhong, WU Yi

“…Routine rehabilitation treatment included stretching training, muscle strength training, joint mobility training, motor control training and functional mobility training for transfer, 40 minutes a time, once a day, five times a week for four weeks; routine walking training included pelvic control training, knee joint stability training, balance training, up and down stairs training, 20 minutes a time, once a day, five times a week for four weeks. …”
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A meta-analysis of randomized controlled trials: evaluating the efficacy of isokinetic muscle strengthening training in improving knee osteoarthritis outcomes by Wanqin Guo, Jingyang Gao, Dawazhuoma, Xiuling Mi, Ciwang, Bianba

“…The studies included were randomized controlled trials (RCTs) with participants from the plains who met the diagnostic criteria for KOA as outlined in the 2019 edition, with no restrictions on gender, age, or disease course, and no patients with advanced disease; studies where in the control group was either a non-intervention group or a group receiving treatment, other than IMST, and the experimental group received IMST alone or in addition to the treatment administered to the control group; and studies with at least two of the following outcome indicators: (i) knee flexors (Flex)/extensors (Ext) peak torque (PT), (ii) knee Flex/Ext total work (TW), (iii) knee Flex/Ext max rep total work (MRTW), (iv) knee Flex/Ext average power (AP), (v) visual analogue scale (VAS) for pain, (vi) Lequesne index (LI), (vii) Western Ontario and McMaster University Osteoarthritis Index (WOMAC), (viii) Lysholm Knee Scoring Scale (LKSS), (ix) range of motion (ROM) of the knee joint, and (x) 6-min walk test. We systematically reviewed the RCTs in both Chinese and English and evaluated the quality of the included literature. …”
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Effect of Zhuanggu Jianxi Decoction on Synovitis in Knee Osteoarthritis Rabbits via LXRs/NF-κB Pathway by CHEN Peng, GUO Jiemei, XIAO Yan, WANG Jianhui, SU Youxin

“…The KOA model was injected with papain into the knee joint of the right hind limb. After the model was identified successfully, the synovial tissue of the right hind limb knee was cut into small pieces with a size about 1-2 mm³ under aseptic operation, 60 pieces were collected and randomly divided into model group, Zhuanggu Jianxi decoction group, LXRα inhibitor group and N-CoR inhibitor group with 15 pieces each, the synovial tissue pieces in each group were cultured in 6 well plates with every 3 pieces in each well, the four groups were intervened with 10% blank serum, 10% drug-containing serum, 10% drug-containing serum+5CPPSS-50 and 10% drug-containing serum+ML-792 respectively; the synovium of the blank group was prepared by the same method and cultured with 10% blank serum for 5 wells. …”
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Safety and efficacy of mesenchymal stromal cells mitochondria transplantation as a cell-free therapy for osteoarthritis by Ana Maria Vega-Letter, Cynthia García-Guerrero, Liliana Yantén-Fuentes, Carolina Pradenas, Yeimi Herrera-Luna, Eliana Lara-Barba, Felipe A. Bustamante-Barrientos, Masyelly Rojas, María Jesús Araya, Nicole Jeraldo, Constanza Aros, Francisca Troncoso, Daniela Poblete, Angela Court, Alexander Ortloff, Jose Barraza, Francesca Velarde, Carlos Farkas, Claudio Carril, Noymar Luque-Campos, Gonzalo Almarza, Maximiliano Barahona, Jose Matas, Lucas Cereceda, Rocío Lorca, Jorge Toledo, Karina Oyarce, Rolando Vernal, Andrés Caicedo, Andrea del Campo, Yessia Hidalgo, Roberto Elizondo-Vega, Farida Djouad, Maroun Khoury, Fernando E. Figueroa, Patricia Luz-Crawford

“…Furthermore, Mito-MSC was detected within the knee joint for up to 24 h post-injection without eliciting an inflammatory response in CIOA mice. …”
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Cell-Free Strategies for Repair and Regeneration of Meniscus Injuries through the Recruitment of Endogenous Stem/Progenitor Cells by Weimin Guo, Wenjing Xu, Zhenyong Wang, Mingxue Chen, Chunxiang Hao, Xifu Zheng, Jingxiang Huang, Xiang Sui, Zhiguo Yuan, Yu Zhang, Mingjie Wang, Xu Li, Zehao Wang, Jiang Peng, Aiyuan Wang, Yu Wang, Shuyun Liu, Shibi Lu, Quanyi Guo

“…The meniscus plays a vital role in protecting the articular cartilage of the knee joint. The inner two-thirds of the meniscus are avascular, and injuries to this region often fail to heal without intervention. …”
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