Exploring the Anti-PANoptosis Mechanism of Dachaihu Decoction Against Sepsis-Induced Acute Lung Injury: Network Pharmacology, Bioinformatics, and Experimental Validation by Yang Z, Kao X, Zhang L, Huang N, Chen J, He M

“…Enrichment analysis showed that DCHD against SALI via anti-PANoptosis by modulating tumor necrosis factor (TNF), AGE-RAGE, phosphoinositide 3-kinase (PI3K)-AKT, and Toll-like receptor signaling pathways by targeting Casp3, cellular tumor antigen p53 (TP53), B-cell lymphoma 2 (Bcl2), toll-like receptor-4 (TLR4), STAT3, STAT1, RELA, NF-κB1, myeloid cell leukemia-1 (MCL1), JUN, IL-1β, HSP90AA1, Casp9, Casp8, and Bcl2l1. …”
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Targeting the hERG1/β1 integrin complex in lipid rafts potentiates statins anti-cancer activity in pancreatic cancer by Claudia Duranti, Jessica Iorio, Valeria Manganelli, Giacomo Bagni, Rossella Colasurdo, Tiziano Lottini, Michele Martinelli, Chiara Capitani, Giulia Boso, Franco Nicolas D’Alessandro, Maurizio Sorice, Andrea Becchetti, Roberta Misasi, Tina Garofalo, Annarosa Arcangeli

“…The complex recruits the p85 subunit of phosphatidyl-inositol-3-kinase (PI3K), activating phosphoinositide metabolism and triggering an intracellular signaling pathway centered on Akt. …”
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Protective Effects of NaHS/miR-133a-3p on Lipopolysaccharide-Induced Cardiomyocytes Injury by Yi-Mei Jin, Ai-Rong Huang, Mei-qian Yu, Wan-Ding Ye, Xiao-guang Hu, Hua-min Wang, Zhi-wei Xu, Dong-shi Liang

“…The adenosine triphosphate (ATP) kit was used to detect ATP content; real-time quantitative polynucleotide chain reaction (qPCR) was used to measure the levels of mammalian targets of rapamycin (mTOR), AMP-dependent protein kinase (AMPK), and miR-133a-3p, and Western blot (WB) was used to detect protein levels of mTOR, AMPK, myosin-like Bcl2 interacting protein (Beclin-1), microtubule-associated protein 1 light chain 3 (LC3I/II), and P62 (sequestosome-1, sqstm-1/P62). …”
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PARylation of HMGA1 desensitizes esophageal squamous cell carcinoma to olaparib by Xin‐Yuan Lei, Kai‐Yue He, Qiu‐Tong Li, Lei Zhang, Dan‐Hui Wu, Jing‐Yu Yang, Jin‐Rong Guo, Meng‐Jie Liu, Zi‐Long Zhao, Jun‐Qi Li, Huai Liu, Yuan Zhao, Yu‐Jia Li, Qian‐Hui Sun, Chen‐Guang Wu, Yun‐Fan Wang, Geng‐Sheng Cao, Gang Wang, Yong‐Ping Jian, Zhi‐Xiang Xu

“…This modification enhances the accumulation of Ku70/Ku80 at the site of DNA damage and activates the DNA‐dependent protein kinase catalytic subunit, facilitating nonhomologous end‐joining repair. …”
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Clinical characteristics and predictive biomarkers of intensive care unit-acquired weakness in patients with cardiogenic shock requiring mechanical circulatory support by Tae Higuchi, Tomomi Ide, Takeo Fujino, Takeshi Tohyama, Yuta Nagatomi, Tomoyuki Nezu, Masataka Ikeda, Toru Hashimoto, Shouji Matsushima, Keisuke Shinohara, Masaaki Nishihara, Takeshi Iyonaga, Tomohiko Akahoshi, Tomoki Ushijima, Akira Shiose, Shintaro Kinugawa, Hiroyuki Tsutsui, Kohtaro Abe

“…The ICU-AW group showed a higher prevalence of extracorporeal membrane oxygenation and ventilator use. Creatine kinase, troponin T, interleukin (IL)-15 levels on admission were significantly higher, whereas hemoglobin and albumin levels were significantly lower in the ICU-AW group. …”
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Integrating traditional biomarkers and emerging predictors to assess neoadjuvant chemotherapy efficacy in breast cancer: a multifactorial analysis of Ki-67, CDK4, EGFR, TILs and ct... by Tianzhao Du, Ye Yuan, Shulan Sun, Zhichao Gao, Xiaoshuai Li

“…Abstract Objective This study aimed to analyse the correlation between the expression of cell proliferation-associated antigen (Ki-67), cell cycle protein-dependent kinase 4 (CDK4), epidermal growth factor receptor (EGFR), tumour-infiltrating lymphocytes (TILs) and circulating tumour DNA (ctDNA) with the outcome and prognosis of patients with breast cancer (BC) undergoing neoadjuvant chemotherapy (NACT). …”
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A conformational change of C-reactive protein drives neutrophil extracellular trap formation in inflammation by Ebru Karasu, Rebecca Halbgebauer, Lena Schütte, Johannes Greven, Felix M. Bläsius, Johannes Zeller, Oscar Winninger, David Braig, David Alexander Christian Messerer, Bettina Berger, Hendrik Feuerstein, Anke Schultze, Karlheinz Peter, Uwe Knippschild, Klemens Horst, Frank Hildebrand, Steffen U. Eisenhardt, Markus Huber-Lang

“…We found mCRP-induced neutrophil-extracellular trap formation in vitro and ex vivo involving nicotinamide adenine dinucleotide phosphate oxidase activation, p38/mitogen-activated protein kinase signaling, and histone H3 citrullination. Mimicking the trauma milieu in a human ex vivo whole blood model, we found significant mCRP generation as well as NET formation, prevented by blocking pCRP conformational changes. …”
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Mechanism of <italic>Coptis chinensis</italic> in Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology and Molecular Docking by LIU Huiling, TAN Dingying, HUANG Min, CHEN Pingping, ZHANG Wang

“…It can act on mitogen-activated proteinase 1 (MAPK1), serine/threonine protein kinase (AKT1), tumor protein p53 (TP53), transcription factor AP-1 (JUN), tumor necrosis factor (TNF), interleukin-6 (IL-6), which can regulate MAPK, AGEs-RAGE, PI3K-Akt, Toll-like receptors, HIF-1, TNF signaling pathways, etc. ④ The molecular docking results showed that, the molecular docking affinity between the active ingredients of <italic>Coptis chinensis</italic> and the above core targets was less than -7.0 kcal/mol (1 kca/mol=4.186 kJ/mol), and the binding activity was better.Conclusion<italic>Coptis chinensis</italic> may play an important role in the treatment of type 2 diabetes mellitus and its complications by regulating glucose and lipid metabolism, inflammatory response, cell proliferation and apoptosis and promoting oxidative stress, etc.…”
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Integration of metabolomic and transcriptomic analyses reveals regulatory functions of the ChREBP transcription factor in energy metabolism by Jie An, Inna Astapova, Guofang Zhang, Andrew L. Cangelosi, Olga Ilkayeva, Hannah Marchuk, Michael J. Muehlbauer, Tabitha George, Joseph Brozinick, Mark A. Herman, Christopher B. Newgard

“…Despite suppression of pyruvate kinase, pyruvate levels are maintained, possibly via increased expression of pyruvate and amino acid transporters. …”
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Nox1/PAK1 is required for angiotensin II-induced vascular inflammation and abdominal aortic aneurysm formation by Hui He, Tianyu Jiang, Meng Ding, Yuan Zhu, Xiaoting Xu, Yashuang Huang, Wenfeng Yu, Hailong Ou

“…Further, we found the Nox1 protein interacted with p21-activated kinase 1 (PAK1) in Ang II-stimulated VSMCs. The PAK1, controlled by Nox1/ROS, promoted VSMC proliferation, migration and differentiation; this is associated with increased activities of vimentin and cofilin, and cytoskeleton modulation. …”
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Cellular Cholesterol Loss Impairs Synaptic Vesicle Mobility via the CAMK2/Synapsin-1 Signaling Pathway by Atikam Qurban, Mengqi Zhang, Hengbing Zu, Kai Yao

“…Additionally, we performed immunoblotting to quantify the expression of DHCR24, total calmodulin-dependent protein kinase 2 (CAMK-2), p-CAMK2 (T286), caveolin-1, total synapsin-1, phosphorylated synapsin-1 (p-synapsin-1; S605), and synaptophysin in each experimental group. …”
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Loss of Lkb1 cooperates with BrafV600E and ultraviolet radiation, increasing melanoma multiplicity and neural‐like dedifferentiation by Kimberley McGrail, Elena González‐Sánchez, Paula Granado‐Martínez, Roberto Orsenigo, Yuxin Ding, Berta Ferrer, Javier Hernández‐Losa, Iván Ortega, Juan Martín‐Caballero, Eva Muñoz‐Couselo, Vicente García‐Patos, Juan A. Recio

“…Analysis of human melanoma tumors [data from The Cancer Genome Atlas (TCGA)] revealed that 50% or more of the samples expressed no or low amounts of serine/threonine protein kinase STK11 (also known as LKB1) protein. Here, we report that, in a mouse model, concomitant neonatal BrafV600E activation and Lkb1 tumor suppressor ablation in melanocytes led to full melanoma development. …”
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ROS-ERK Pathway as Dual Mediators of Cellular Injury and Autophagy-Associated Adaptive Response in Urinary Protein-Irritated Renal Tubular Epithelial Cells by Jian-kun Deng, Xueqin Zhang, Hong-luan Wu, Yu Gan, Ling Ye, Huijuan Zheng, Zebing Zhu, Wei Jing Liu, Hua-feng Liu

“…ERK, an extracellular signal-regulated protein kinase, is involved in various biological responses, such as cell proliferation and differentiation, cell morphology maintenance, cytoskeletal construction, apoptosis, and canceration of cells. …”
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The StbHLH47 transcription factor negatively regulates drought tolerance in potato (Solanum tuberosum L.) by Peijie Wang, Xiaojuan Wu, Nan Li, Hushuai Nie, Yu Ma, Juan Wu, Zhicheng Zhang, Yanhong Ma

“…Differential gene expression analysis of wild-type plants (WT) and plants overexpressing StbHLH47 (OE-StbHLH47) under drought stress revealed that the significantly differentially expressed genes were enriched in metabolic pathways, biosynthesis of various plant secondary metabolites, biosynthesis of metabolites, plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signalling pathway-plant, phenylpropanoid biosynthesis, and plant‒pathogen interactions. …”
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Multicentre, 26-week, open-label phase 2 trial of the JAK inhibitor filgotinib in Behçet’s disease, idiopathic inflammatory myopathies and IgG4-related disease: DRIMID study protoc... by Sander W Tas, Joost Raaphorst, Paco M J Welsing, Jacob M van Laar, Rogier M Thurlings, Robbert J Goekoop, César Magro Checa, Jan A M van Laar, Bettina C Geertsema-Hoeve, Nicolle H Rekers, Erik Present

“…Given the activation of the type 1 interferon pathway in BD, IIM and IgG4-RD, inhibition of the Janus kinase (JAK) STAT pathway emerges as a promising therapeutic strategy. …”
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Functional Remediation Improves Serum BDNF and TrkB Levels in Euthymic Patients with Bipolar Disorder: A Randomized Trial Study by Li R, Li J, Ji SS, Li D, Chu L, Zhang J, Sun X, Luo X, Zhang Y

“…Ru Li,1,&ast; Jiaxin Li,1,&ast; Shiyi Suzy Ji,2 Dazhi Li,1 Lijun Chu,1 Jian Zhang,1 Xia Sun,1 Xingguang Luo,3 Yong Zhang1 1Unit of Bipolar Disorder, Tianjin Anding Hospital, Tianjin, People’s Republic of China; 2Department of Counseling and Clinical Psychology, Teachers College, Columbia University, NY, New York, USA; 3Department of Psychiatry, Yale University School of Medicine, New Haven, CT, USA&ast;These authors contributed equally to this workCorrespondence: Yong Zhang, Unit of Bipolar Disorder, Tianjin Anding Hospital, 13 Liulin Road, Hexi District, Tianjin, 300222, People’s Republic of China, Tel/Fax +86 22 8818 8258, Email zhangyong@tjmhc.comPurpose: We aimed to verify the impact of functional remediation (FR) on serum brain-derived neurotrophic factor (BDNF) and tyrosine kinase receptor B (TrkB) levels, to explore the biomechanism of FR intervention in patients with euthymic bipolar disorder (BD).Patients and Methods: This is a randomized controlled, 12-week intervention study with participants randomized into the FR group (n=39) and the treatment as usual group (TAU, n=42) at the 1∶1 ratio. 17-Hamilton Depression Rating Scale-17 (HDRS-17), Young Mania Rating Scale (YMRS), and Measurement and Treatment Research to Improve Cognition in Schizophrenia (MATRICS) Consensus Cognitive Battery (MCCB) were used to assess affective symptoms and cognitive functioning both at baseline and week 12, respectively. …”
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Modified single-incision MIS-TLIF with expandable tubular assistance for degenerative lumbar spine diseases by Wenlong Hu, Wenlong Hu, Fei He, Fei He, Kai Sun, Kai Sun, Haiwu Wan, Sijun Ruan, Bo Huang

“…Laboratory results [whole blood C-reactive protein (CRP), serum creatine kinase (CK)] and clinical outcomes [VAS scores for low back and leg pain, Oswestry Disability Index (ODI), excellent and good rate according to the modified MacNab criteria, and interbody fusion rate according to the Brantigan criteria] were also evaluated.ResultThere were no significant differences in the basic demographics between the two groups. …”
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Research on screening key proteins related to the pathogenesis of sepsis associated encephalopathy based on DIA proteomics technology by Wu Shuhui, Hu Hongjie, Lu Yuru, Zhu Wei

“…These proteins were involved in signaling pathways such as Mitogen-Activated Protein Kinase （MAPK） signaling, focal adhesion and phospholipase D signaling pathways, and were involved in biological processes such as cell adhesion, signaling, macromolecular modification, etc. …”
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Correlation Analysis of the Entorhinal Cortex-Hippocampus Nerve Fiber Changes and Memory Dysfunction in P301L-Tau Mouse Model and Molecular Mechanism by CHEN Xiaocheng, LIANG Shengxiang, LIN Bingbing, LIU Weilin, HUANG Jia

“…Western blot was used to detect the expression levels of t-Tau, p-Tau (Ser262), and glycogen synthase kinase-3β (GSK3β). Pearson correlation analysis was used to analyze the correlation between fractional anisotropy (FA) values in different brain regions of the entorhinal cortex-hippocampus and their corresponding learning and memory functions.Results(1) Learning and memory function test: compared with the control group, the learning and memory function of the model group significantly decreased, the escape latency significantly increased, the number of platform crossings and time ratio of target quadrant significantly decreased (<italic>P</italic>&lt;0.05). (2) Structure of nerve fibers in the entorhinal cortex and hippocampus: compared with the control group, FA values of the entorhinal cortex and hippocampus in the model group decreased more significantly, MD values of the entorhinal cortex in the model group increased more significantly, while there was no significant difference in the MD values of the hippocampus in the model group (<italic>P</italic>&gt;0.05). (3) Correlation analysis between FA values in the entorhinal cortex-hippocampus and learning and memory function: FA values in the entorhinal cortex and hippocampus were positively correlated with the number of platform crossings (<italic>r</italic>=0.76, <italic>P</italic>&lt;0.05; <italic>r</italic>=0.62, <italic>P</italic>&lt;0.05). (4) Expression of t-Tau, Ser262 and GSK3β protein in the entorhinal cortex-hippocampus: compared with the control group, the expression of t-Tau, p-Tau (Ser262) and GSK3β protein of entorhinal cortex and hippocamp increased more significantly in the model group (<italic>P</italic>&lt;0.05).ConclusionThe Tau protein in the entorhinal cortex-hippocampus of the P301LTau mouse model was hyperphosphorylated, which leads to destruction of the nerve fiber structures in the entorhinal cortex-hippocampus and learning and memory dysfunctions. …”
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Neuroligin 1 Regulates Autistic‐Like Repetitive Behavior through Modulating the Activity of Striatal D2 Receptor‐Expressing Medium Spiny Neurons by Dandan Lv, An Liu, Ziyue Yi, Mingdao Mu, Miao Wu, Xingcan Li, Kun Cao, Ruining Liu, Zhengping Jia, Junhai Han, Wei Xie

“…Finally, through single‐nucleus RNA sequencing (sn‐RNAseq) and protein detection verification, it is revealed that the overactivation of protein kinase C (PKC) in Nlgn1‐deficient mice contributes to excessive repetitive behaviors and increased neuronal excitability. …”
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