Stimulation of Alpha7 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Upregulation of MMP, MCP-1, and RANTES through Modulating ERK1/2/AP-1 Signaling Pathway in RAW264... by Liping Liu, Hongxian Wu, Qunan Cao, Zhenzhen Guo, Anmin Ren, Qiuyan Dai

“…Nicotine markedly stimulated the phosphorylation of extracellular signal-regulated kinase1/2 (ERK1/2) and c-Jun in RAW264.7 cells. Pretreatment with U0126 significantly suppressed phosphorylation of ERK1/2 and further attenuated nicotine-induced activation of c-Jun and upregulation of MMP-2, MMP-9, monocyte chemotactic protein- (MCP-) 1, and regulated upon activation normal T cell expressed and secreted (RANTES). …”
Get full text

The Impact of Targeted Therapies on Red Blood Cell Aggregation in Patients with Chronic Lymphocytic Leukemia Evaluated Using Software Image Flow Analysis by Anika Alexandrova-Watanabe, Emilia Abadjieva, Lidia Gartcheva, Ariana Langari, Miroslava Ivanova, Margarita Guenova, Tihomir Tiankov, Velichka Strijkova, Sashka Krumova, Svetla Todinova

“…Despite advances in therapies targeting Bruton’s tyrosine kinase and anti-apoptotic protein BCL-2, little is known about their effect on red blood cell (RBC) aggregation in blood flow. …”
Get full text

Tofacitinib and budesonide treatment affect stemness and chemokine release in IBD patient-derived colonoids by Arun Sridhar, Ingunn Bakke, Shreya Gopalakrishnan, Nimo Mukhtar Mohamud Osoble, Emilie Prytz Hammarqvist, Henrik P. Sahlin Pettersen, Arne Kristian Sandvik, Ann Elisabet Østvik, Marianne Doré Hansen, Torunn Bruland

“…We investigated cellular effects of pan-Janus Kinase (JAK) inhibitor tofacitinib and the corticosteroid budesonide on uninflamed and TNF + Poly(I:C) stimulated human colon organoids (colonoids) from healthy donors and IBD-patients. …”
Get full text

Functional analysis of the PcCDPK5 gene in response to allelopathic substances on p-hydroxybenzoic acid (p-HBA) stress in patchouli by Xiaofeng Liu, Jingru Zhou, Muhammad Zeeshan Ul Haq, Zhineng Fu, Guangtao Gu, Jing Yu, Ya Liu, Dongmei Yang, Huageng Yang, Yougen Wu

“…Calcium-dependent protein kinase (CDPK) is an important mediator for Ca2 + signal recognition and transduction, playing a crucial role in plant stress response. …”
Get full text

Muramyl Dipeptide Enhances Lipopolysaccharide-Induced Osteoclast Formation and Bone Resorption through Increased RANKL Expression in Stromal Cells by Masahiko Ishida, Hideki Kitaura, Keisuke Kimura, Haruki Sugisawa, Tomo Aonuma, Haruhiko Takada, Teruko Takano-Yamamoto

“…MDP also enhanced LPS-induced mitogen-activated protein kinase (MAPK) signaling, including ERK, p38, and JNK, in stromal cells. …”
Get full text

Selective JAK2 pathway inhibition enhances anti-leukemic functionality in CD19 CAR-T cells by Kohei Mitsuno, Masaya Suematsu, Yuki Naito, Azusa Mayumi, Hideki Yoshida, Shinya Osone, Toshihiko Imamura, Yozo Nakazawa, Shigeki Yagyu, Tomoko Iehara

“…While CD19-targeted CAR-T cells and Janus kinase (JAK) inhibitors have independently shown efficacy against certain B-cell leukemias, such as Philadelphia chromosome-like acute lymphoblastic leukemia, the concurrent use of JAK1/2 inhibitors, such as ruxolitinib, has been implicated in reducing CAR-T cell potency by inhibiting the JAK1-dependent T cell activation pathway. …”
Get full text

Inflammation and Immune Escape in Ovarian Cancer: Pathways and Therapeutic Opportunities by Liu C, Yin Q, Wu Z, Li W, Huang J, Chen B, Yang Y, Zheng X, Zeng L, Wang J

“…COX-2 inhibitors, Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway blockers, and NF-kB inhibitors have shown potential in preclinical studies, while immune checkpoint inhibitors targeting PD-1/PD-L1 and CTLA-4 have been explored with mixed results in OC. …”
Get full text

Integrative proteomic-transcriptomic analysis revealed the lifestyles of Lactobacillus paracasei H4-11 and Kluyveromyces marxianus L1-1 under co-cultivation conditions by Na Liu, Likang Qin, Haiying Zeng, Anyan Wen, Song Miao

“…In L. paracasei H4-11, L-lactate dehydrogenase, phosphoglucomutase, acetate kinase, alcohol dehydrogenase and acetyl-CoA were up-regulated and D-lactate dehydrogenase and N-Acetyltransferase were down-regulated. …”
Get full text

RIPK3-Mediated Necroptosis and Neutrophil Infiltration Are Associated with Poor Prognosis in Patients with Alcoholic Cirrhosis by Zhenzhen Zhang, Guomin Xie, Li Liang, Hui Liu, Jing Pan, Hao Cheng, Hua Wang, Aijuan Qu, Yan Wang

“…Recent data suggests that receptor-interacting protein kinase-3- (RIPK3-) mediated necroptosis plays an important role in alcoholic cirrhosis. …”
Get full text

Mutations in the <i>A34R</i> gene increase the immunogenicity of vaccinia virus by S. N. Shchelkunov, T. V. Bauer, S. N. Yakubitskiy, A. A. Sergeev, A. S. Kabanov, S. A. Pyankov

“…The gene for thymidine kinase is frequently used for insertion/inactivation of foreign genes and it causes virus attenuation. …”
Get full text

Phenazine biosynthesis-like domain-containing protein (PBLD) and Cedrelone promote antiviral immune response by activating NF-ĸB by Peili Hou, Hongchao Zhu, Fengyun Chu, Yan Gao, Xiaonan Sun, Fuzhen Zhang, Xiaomeng Wang, Yueyue Feng, Xingyu Li, Yu Liu, Jun Wang, Xiaoyun Wang, Daniel Chang He, Hongmei Wang, Hongbin He

“…Mechanistically, PBLD activates NF-κB signaling pathway during viral infection via blocking tripartite motif containing 21 (TRIM21)-mediated phosphorylated inhibitory kappa B kinase beta (IKKβ) degradation. Furthermore, we show Cedrelone inhibits viral replication by increasing the PBLD protein expression and subsequently activating NF-κB-mediated IFN-I response. …”
Get full text

Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study by Ivana Resanovic, Zoran Gluvic, Bozidarka Zaric, Emina Sudar-Milovanovic, Aleksandra Jovanovic, Davorka Milacic, Radmilo Isakovic, Esma R. Isenovic

“…Expression of iNOS and phosphorylation of p65 subunit of nuclear factor-κB (NFκB-p65), extracellular-regulated kinases 1/2 (ERK1/2), and protein kinase B (Akt) were examined in lymphocyte lysates by Western blot. …”
Get full text

Construction and Investigation of MicroRNA-mRNA Regulatory Network of Gastric Cancer with Helicobacter pylori Infection by Ping Yang, Junjie Liu, Tianci Yang, Lei Zhang, Peiyou Gong, Boqing Li, Xiuzhi Zhou

“…Results. 22 DEMs and 68 DEGs were identified in GSE197694 and GSE27411 dataset. 16 miRNAs and 509 genes were involved in the expression network, while the cross-genes of the network were mainly enriched in MAP kinase (MAPK) signaling pathway and TGF-beta signaling pathway. …”
Get full text

Repression of PFKFB3 sensitizes ovarian cancer to PARP inhibitors by impairing homologous recombination repair by Yinan Xiao, Yu Wu, Qilong Wang, Mo Li, Chaolin Deng, Xiaoyang Gu

“…This study investigated the role of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3), a pivotal regulator of glycolysis, in PARPi resistance and explored its potential as a therapeutic target to overcome PARPi resistance. …”
Get full text

Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice by Yingqun Zhou, Weiqi Dai, Chunlei Lin, Fan Wang, Lei He, Miao Shen, Ping Chen, Chenfen Wang, Jie Lu, Ling Xu, Xuanfu Xu, Chuanyong Guo

“…Necrostatin-1 (Nec-1) inhibits receptor-interacting protein 1 (RIP1) kinase and programmed necrosis. This study was designed to examine the protective effects and mechanisms of Nec-1 in concanavalin A- (ConA-) induced hepatitis in mice. …”
Get full text

Osteoblast‐Derived ECM1 Promotes Anti‐Androgen Resistance in Bone Metastatic Prostate Cancer by Xinwen Wang, Min Wang, Qijun Lin, Lixin He, Baolin Zhang, Xin Chen, Guanhong Chen, Hong Du, Chuandong Lang, Xinsheng Peng, Yuhu Dai

“…This event further recruits adapter proteins including growth factor receptor‐bound protein 2 (GRB2) and son of sevenless homolog 1 (SOS1), which activates the downstream mitogen‐activated protein kinase (MAPK) signaling pathway to induce anti‐androgen resistance. …”
Get full text

Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAK/STAT Pathway in Human Pancreatic β Cells by Akiyo Yamauchi, Asako Itaya-Hironaka, Sumiyo Sakuramoto-Tsuchida, Maiko Takeda, Kiyomi Yoshimoto, Tomoko Miyaoka, Takanori Fujimura, Hiroki Tsujinaka, Chikatsugu Tsuchida, Hiroyo Ota, Shin Takasawa

“…Promoter assay revealed that a signal transducer and activator of transcription- (STAT-) binding site in each promoter of REG Iα (TGCCGGGAA) and REG Iβ (TGCCAGGAA) was essential for the IL-6+Dx-induced promoter activation. A Janus kinase 2 (JAK2) inhibitor significantly inhibited the IL-6+Dx-induced REG Iα and REG Iβ transcription. …”
Get full text

Inhibition of TFF3 synergizes with c-MET inhibitors to decrease the CSC-like phenotype and metastatic burden in ER+HER2+ mammary carcinoma by Chuyu He, Xuejuan Wang, Yi-Shiou Chiou, Basappa Basappa, Tao Zhu, Vijay Pandey, Peter E. Lobie

“…Furthermore, a high-throughput combinatorial anti-cancer compound library screening revealed that AMPC preferentially synergized with receptor tyrosine kinase c-MET inhibitors (c-METis) to reduce cell survival and the CSC-like phenotype. …”
Get full text

Epigenetics of suicidal behavior by R. N. Mustafin, A. V. Kazantseva, R. F. Enikeeva, Yu. D. Davydova, S. B. Malykh, V. V. Viktorov, E. K. Khusnutdinova

“…The analysis of a brain obtained from suicide victims, representing a unique tool for the analysis of modified genomic processes, revealed a wide range of reprogramming patterns of DNA methylation in promoters of the genes of polyamine (OAZ1, OAZ2, AMD1, ARG2, SKA2), serotonergic (SLC6A4) and GABAergic (GABRA1) systems, HPA-axis (GR, NR3C1), tyrosine kinase (TrkB) receptors, brain-derived neurotrophic factor (BDNF). …”
Get full text

Interfacial cfDNA Enrichment and Amplification with On‐Chip Thermoplasmonics for Highly Sensitive Cancerous Liquid Biopsy by Danhua Wang, Linlin Liu, Wenjing Chi, Zhenping Liu, Jiayun Wu, Yirou Liang, Fei He, Ruixiang Zhang, Pengxin Huang, Yunbo Li, Guangyu Qiu

“…Typical cfDNA biomarkers, including epidermal growth factor receptor (EGFR), tumor protein 53 (TP53), phosphatase and tensin homologue deleted on chromosome 10 (PTEN), and cyclin‐dependent kinase inhibitor (CDKN2A), are quantified with detection limits down to femtomolar‐level. …”
Get full text