Progression of ampC amplification during de novo amoxicillin resistance development in E. coli by Luyuan Nong, Martijs Jonker, Wim de Leeuw, Meike T. Wortel, Benno ter Kuile

“…The amoxicillin-resistant ∆ampC did not show amplification of the fragment around the original ampC position but exhibited a large duplication or triplication at another position, suggesting the essential role of the duplicated genes in resistance development.IMPORTANCEAmoxicillin is the most used antimicrobial against bacterial infections. DNA fragments containing ampC are amplified upon prolonged and stepwise increasing exposure to amoxicillin, causing resistance. …”
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Maternal and neonatal outcomes of Group B Streptococcus colonization: a retrospective study by Guixiu Jin, Lanhua Liu, Xiaolong Wang, Junling Fei, Zhenling Zhu, Ziyan Jiang, Min Liu

“…Abstract Background Group B Streptococcus (GBS) colonization is one of the major causes of severe neonatal infections. The study was intended to identify GBS colonization in pregnant women, explore its potential risk factors, and analyze the impact of GBS on outcomes for both mothers and newborns. …”
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Molecular genetic detection and differentiation of <i>Xanthomonas oryzae</i> pv. <i>oryzicola</i>, bacterial leaf streak agents of rice by M. L. Koroleva, S. A. Blinova, A. A. Shvartsev, V. E. Kurochkin, Ya. I. Alekseev

“…The genus Xanthomonas comprises phytopathogenic bacteria which infect about 400 host species, including a wide variety of economically important plants. …”
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Assessment of phytochemical screening, antibacterial, analgesic, and antipyretic potentials of Litsea glutinosa (L.) leaves extracts in a mice model. by Zubair Khalid Labu, Samira Karim, Md Tarekur Rahman, Md Imran Hossain, Sarder Arifuzzaman, Md Shakil

“…<h4>Background</h4>Litsea glutinosa (LG) leaves have been traditionally used in ethnomedicine for the treatment of various ailments, including pain, fever, and microbial infections. This study aims to scientifically evaluate the therapeutic potential of cold methanol extracts of LG leaves, specifically focusing on their analgesic, antipyretic, and antibacterial activities. …”
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Modeling Coupled Nonlinear Multilayered Dynamics: Cyber Attack and Disruption of an Electric Grid by Marc Mangel, Jimmie McEver

“…In analogy to disease models in which individuals are susceptible, infected, or recovered, in the cyber system, components can be uncompromised and vulnerable to attack, uncompromised and temporarily invulnerable to attack, compromised, or reset and thus not able to contribute to the performance of the physical system. …”
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Seasonal Dynamics of Sand Flies (Diptera, Pshycodidae), Vectors of Cutaneous Leishmaniasis, in the City of Fez, Northern Morocco by Najoua Darkaoui, Abdellatif Janati Idrissi, Fatima Zahra Talbi, Youness El Fattouhi, Hajar El Omari, Mohamed Najy, Abdelkarim Taam, Abdellatif Alami, Fouad El-Akhal, Abdelhakim El Ouali Lalami

“…The infections transmitted by sand flies (Diptera, Psychodidae) pose always a real health problem due to the increasing number of cases detected each year and the annual emergence of new leishmaniasis outbreaks. …”
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Trends in the utilisation of maternal and child healthcare services from the public and private health sectors in India, 2005–2021: an analysis of cross-sectional survey data by Niranjan Saggurti, Rajib Acharya, Abhishek Kumar, Anchal Purbey, Arupendra Mozumdar, Punit Mishra

“…Market sustainability of key MCH indicators was assessed by level of MCH services and subsidisation.Participants 36 850, 190 898 and 176 843 ever-married women aged 15–49 years, 4440, 22 500 and 15 334 children under 5 years of age with diarrhoea before the survey, and 2552, 6960 and 6117 children with symptoms of acute respiratory infections (ARI) in NFHS 2005–2006, 2015–2016 and 2019–2021, respectively.Outcome measures The study used three maternal healthcare indicators: women had four or more antenatal care (ANC) visits, had institutional delivery, and received postnatal care (PNC); and two child healthcare indicators: care seeking for ARI and diarrhoea.Results In India, utilisation of maternal healthcare services increased over the last 15 years: four or more ANC visits increased from 37% to 58% and PNC of mothers increased from 33% to 78% between 2005–2006 and 2019–2021. …”
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hCTLA4-Gene-Modified Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMMSCs) Maintain POSTN Secretion to Enhance the Migration Capability of Allogeneic hBMMSCs through the Integ... by Lei Song, Fei Zhang, Rui Zhou, Jun Xiao, Lei He, Fei Dai

“…In the present study, we investigated whether CTLA4-modified hBMMSCs are involved in the migration of allogeneic hBMMSCs (allo-hBMMSCs) by maintaining POSTN secretion. hBMMSCs were isolated from different groups, named hBMMSCs and allo-hBMMSCs. hBMMSCs that were infected with the negative control (NC), empty adenovirus- or recombinant adenovirus-expressing CTLA4, POSTN, or CTLA4 plus the shRNA of POSTN were named NC hBMMSCs, CTLA4-modified hBMMSCs, POSTN-modified hBMMSCs, or CTLA4+shPOSTN-modified hBMMSCs, respectively. …”
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Mono-allelic epigenetic regulation of polycistronic transcription initiation by RNA polymerase II in Trypanosoma brucei by Rudo Kieft, Laura Cliffe, Haidong Yan, Robert J. Schmitz, Stephen L. Hajduk, Robert Sabatini

“…Trypanosoma brucei brucei is unable to infect humans because of its susceptibility to an innate immune complex, trypanosome lytic factor (TLF) in the circulation of humans. …”
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Voriconazole Pharmacokinetics Administered at 4 mg/kg IM and IV in Nursehound Sharks (<i>Scyliorhinus stellaris</i>) Under Human Care by Daniela Cañizares-Cooz, Daniel García-Párraga, Sonia Rubio-Langre, Teresa Encinas, Pablo Morón-Elorza

“…The use of azole antifungal drugs, especially voriconazole, has shown promise as a potential treatment option for fungal infections in elasmobranchs, with favorable outcomes in some registered cases. …”
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Study on the protective effect of naringin-regulated lncRNA GAS5 against mitochondrial apoptosis in osteoarthritic chondrocytes by LIN Qin, ZHU Yingkai, LIN Yanming, LAN Shujie, CHEN Changxing, FU Changlong

“…In vitro experiments, Lenti-lncRNA GAS5 was used to infect sodium nitroprusside (SNP)-induced chondrocytes, and the expression of lncRNA GAS5 was detected by fluorescence in situ hybridization in rat chondrocytes; after silencing of lncRNA GAS5, the expression level of lncRNA GAS5 in rat chondrocytes was detected by Real-time PCR; Western blot detected the protein levels of Caspase-3, Bax, MMP-13 and Cyt-C.Results① Pathological changes of cartilage tissue: The articular cartilage matrix in naringenin group was relatively complete, the surface was relatively smooth, the structure was clear, the tide line was more complete, the number of chondrocytes was more full, the arrangement was more orderly, the cartilage staining was more uniform, and the structure was significantly restored than that in the model group. ② lncRNA GAS5 expression in cartilage tissue: Compared with model group, lncRNA GAS5 expression in naringin group was decreased (<italic>P</italic>&lt;0.01). ③ Expression of proteins related to mitochondrial apoptosis in cartilage tissues: Compared with model group, the expressions of Caspase-3, Bax, MMP-13 and Cyt-C in cartilage tissues of naringin group were decreased (<italic>P</italic>&lt;0.01). ④ Fluorescence expression of lncRNA GAS5 in chondrocytes: FISH results showed that the fluorescence expression of lncRNA GAS5 was decreased after naringin treatment of SNP-induced chondrocytes (<italic>P</italic>&lt;0.01). ⑤ The protein expressions of Caspase-3, Bax, MMP-13 and Cyt-C in chondrocytes: Compared with the model group, the protein expressions of Caspase-3, Bax, MMP-13 and Cyt-C in naringin group were significantly decreased (<italic>P</italic>&lt; 0.01). …”
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Practices and knowledge of health personnel about transgenderity in Primary Health Care by Francisco Jean Gomes de Sousa, Edgar Amatuzzi, Andressa Teoli Nunciaroni, Jaime Alonso Caravaca-Morera, Claudia Regina Ribeiro, Adriana Lemos

“…Results: Professionals’ lack of knowledge about policies and terms was evidenced; there were conceptual confusions, inadequate associations, and a restricted approach to the prevention of sexually transmitted infections. Conclusions: The lack of training reflects a cisnormative and binary approach, compromising the assistance to the transgender population. …”
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Australian children’s foot, ankle and leg problems in primary care: a secondary analysis of the Bettering the Evaluation and Care of Health (BEACH) data by Hylton B Menz, Christopher Harrison, Peter A Lazzarini, Cylie M Williams, Julie Gordon

“…The top four most frequently managed problems were injuries (755.9 per 100 000 encounters), infections (458.2), dermatological conditions (299.4) and unspecified pain (176.3). …”
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Synthesis, Antibacterial, Antioxidant, and Molecular Modeling Studies of Novel [2,3′-Biquinoline]-4-Carboxylic Acid and Quinoline-3-Carbaldehyde Analogs by Zeleke Digafie, Yadessa Melaku, Zerihun Belay, Rajalakshmanan Eswaramoorthy

“…Currently, it has been common to see people being affected and dying from untreatable infections caused by multidrug-resistant (MDR) germs. …”
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Using Sequence Mining to Predict Complex Systems: A Case Study in Influenza Epidemics by Theyazn H. H. Aldhyani, Manish R. Joshi, Shahab A. AlMaaytah, Ahmed Abdullah Alqarni, Nizar Alsharif

“…According to the World Health Organisation, three to five million individuals are infected by influenza, and around 250,000 to 500,000 people die of this infectious disease worldwide. …”
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Survival Status and Predictors of Mortality among Multidrug-Resistant Tuberculosis Patients in Saint Peter’s Specialized Hospital, Addis Ababa, Ethiopia by Mikyas Arega Muluneh, Abayneh Birlie Zeru, Behailu Tariku Derseh, Abebaw Molla Kebede

“…The independent predictors of time to death were being anemic (AHR = 3.65; 95% CI: 1.36, 9.79), having clinical complication (AHR = 3; 95% CI: 1.2, 7.5), and being HIV infected (AHR = 5.8; 95% CI: 2.2, 15.7). Conclusions. …”
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Long-Term Safety and Effectiveness of Dimethyl Fumarate in Patients with Multiple Sclerosis Treated in Routine Medical Practice: Final Analysis of the ESTEEM Study by Krupa Shah Pandey, Kathryn Giles, Konstantin Balashov, Richard Macdonell, Jörg Windsheimer, Mikel Martinez, Ivan Božin, Stephanie Raynaud, Matthew Scaramozza, Oksana Mokliatchouk, Zhaonan Sun, Nicholas Belviso, Yayoi Sato, Xiaochen Lin, Annette Okai

“…SAEs occurred in 391 (8%) patients, most commonly infections and infestations (n = 102; 2%). Adjusted ARR declined by 90% (95% confidence interval [CI]: 90–91%; p < 0.0001), from 0.81 (95% CI 0.79–0.84) 12 months before enrollment to 0.08 (95% CI 0.08–0.09) 6 years after enrollment. …”
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Impact of pulmonary yoga module on lung capacity of COVID-19 adult survivors by Adarsh Tomar, Sudhanshu Verma

“…Those with healthy lungs are less likely to get infected by viruses and tend to have better immunity which is the key factor for a healthy life. …”
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Subcutaneous Fat Obesity in a High Body Mass Index Donor Is Not a Contraindication to Living Donor Hepatectomy by Hirak Pahari, Amey Sonavane, Amruth Raj, Anup Kumar Agrawal, Ambreen Sawant, Deepak Kumar Gupta, Amit Gharat, Vikram Raut

“…His indications were refractory ascites, hepatic encephalopathy, acute kidney injury, recurrent elbow and urine infections leading to cachexia. She was initially rejected due to a high BMI but failed to lose weight over the next 2 months, and the need for a transplant in her husband was imminent. …”
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European ILD registry algorithm for self-assessment in interstitial lung diseases (eurILDreg ASA-ILD). by Ekaterina Krauss, Laurenz H Claas, Silke Tello, Jennifer Naumann, Sandra Wobisch, Stefan Kuhn, Raphael W Majeed, Karen Moor, Maria Molina-Molina, Oisin Byrne, Rebecca Borton, Marlies S Wijsenbeek, Nik Hirani, Carlo Vancheri, Bruno Crestani, Andreas Guenther, eurILDreg investigators

“…<h4>Methods</h4>The European ILD Registry Algorithm for Self-Assessment in ILD (eurILDreg ASA-ILD), developed by all eurILDreg principal investigators, includes questionnaires on symptom burden, respiratory infections, and quality of life (EQ5D VAS, K-BILD, LCQ). …”
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