Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening by Yu-hong Wang, Ting-ting Liu, Yan-ping Guo, Shuo-ji Zhu, Zi-ming Liao, Jia-mei Song, Xi-ming Zhu, Jia-liang Liang, Moussa Ide Nasser, Nan-bo Liu, De-hua Chang, Ping Zhu, Bin Yao

“…Although human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and the engineered heart organoid have been used for drug screening and mimicking disease models, they are always limited by the immaturity and lack of functionality of the cardiomyocytes. …”
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Absence of Rybp Compromises Neural Differentiation of Embryonic Stem Cells by Gergo Kovacs, Viktoria Szabo, Melinda K. Pirity

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New Turns for High Efficiency Knock-In of Large DNA in Human Pluripotent Stem Cells by Xiangjun He, Yin-Xiong Li, Bo Feng

“…Recent technological advances by a coupling CRISPR system with various DNA repair mechanisms have further opened up new opportunities to overcome existing challenges in knocking-in foreign DNA in human pluripotent stem cells, including embryonic stem cells (ESC) and induced pluripotent stem cells (iPSC). In this review, we summarized the very recent development of CRISPR-based knock-in strategies and discussed the results obtained as well as potential applications in human ESC and iPSC.…”
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Research advances in the construction of stem cell-derived ovarian organoids by Tianyue Zhang, Mengtong Zhang, Sichen Zhang, Shaowei Wang

“…Constructing ovarian organoids involves two main processes: differentiating induced pluripotent stem cells (iPSCs) into germ and ovarian somatic cells to restore ovarian function and using extracellular matrix (ECM) to create a suitable ovarian microenvironment and scaffold. …”
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Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity by Anjana Nityanandam, Mary H. Patton, Ildar T. Bayazitov, Kyle D. Newman, Kristen T. Thomas, Stanislav S. Zakharenko

“…Summary: Human induced pluripotent stem cells (hiPSCs) can be used to generate assembloids that recreate thalamocortical circuitry displaying short-term and long-term synaptic plasticity. …”
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Engineering aortic valves via transdifferentiating fibroblasts into valvular endothelial cells without using viruses or iPS cells by Peng Tang, Fuxiang Wei, Weihua Qiao, Xing Chen, Chenyang Ji, Wanzhi Yang, Xinyu Zhang, Sihan Chen, Yanyan Wu, Mingxing Jiang, Chenyu Ma, Weiqiang Shen, Qi Dong, Hong Cao, Minghui Xie, Ziwen Cai, Li Xu, Jiawei Shi, Nianguo Dong, Junwei Chen, Ning Wang

“…The technology of induced pluripotent stem cells (iPSCs) has enabled the conversion of somatic cells into primitive undifferentiated cells via reprogramming. …”
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iPSCs: A Minireview from Bench to Bed, including Organoids and the CRISPR System by Andrés Javier Orqueda, Carla Alejandra Giménez, Federico Pereyra-Bonnet

“…Ten years later, the reprogramming process became a defined method of producing induced pluripotent stem cells (iPSCs) through the overexpression of four transcription factors. iPSCs are capable of originating virtually all types of cells and tissues, including a whole new animal. …”
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Structural characterization of stem cell factors Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to detect phospho-dependent binding partners by Bouguechtouli, Chafiaa, Ghouil, Rania, Alik, Ania, Dingli, Florent, Loew, Damarys, Theillet, Francois-Xavier

“…The combined expression of a handful of pluripotency transcription factors (PluriTFs) in somatic cells can generate induced pluripotent stem cells (iPSCs). Here, we report the structural characterization of disordered regions contained in four important PluriTFs, namely Oct4, Sox2, Nanog and Esrrb. …”
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Identification of Molecular Signatures in Neural Differentiation and Neurological Diseases Using Digital Color-Coded Molecular Barcoding by Debora Salerno, Alessandro Rosa

“…Human pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells, represent powerful tools for disease modeling and for therapeutic applications. …”
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Protocol for differentiating cardiomyocytes and generating engineered heart tissues from human feeder-free extended pluripotent stem cells by Zhongjun Wan, Shanshan Wen, Ran Zheng, Li Li, Wei Jiang, Donghui Zhang

“…We describe steps for converting human embryonic stem cells or induced pluripotent stem cells (ESCs/iPSCs) into ffEPSCs, followed by their long-term maintenance, cryopreservation, seed preservation, and differentiation into cardiomyocytes. …”
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Cell Reprogramming, IPS Limitations, and Overcoming Strategies in Dental Bioengineering by Gaskon Ibarretxe, Antonia Alvarez, Maria-Luz Cañavate, Enrique Hilario, Maitane Aurrekoetxea, Fernando Unda

“…The procurement of induced pluripotent stem cells, or IPS cells, from adult differentiated animal cells has the potential to revolutionize future medicine, where reprogrammed IPS cells may be used to repair disease-affected tissues on demand. …”
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zIncubascope: Long-term quantitative imaging of multi-cellular assemblies inside an incubator. by Anirban Jana, Naveen Mekhileri, Adeline Boyreau, Aymerick Bazin, Nadège Pujol, Kevin Alessandri, Gaëlle Recher, Pierre Nassoy, Amaury Badon

“…We exemplify the capabilities of our system by investigating human induced pluripotent stem cells (hiPSCs) enclosed in spherical capsules, hiPSCs in tubular capsules and yeast cells in spherical capsules. …”
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Cholangiocyte organoids for disease, cancer, and regenerative medicine by Munemasa Nagao, Akihisa Fukuda, Hirotaka Kashima, Sho Matsuyama, Kei Iimori, Shinnosuke Nakayama, Kenta Mizukoshi, Munenori Kawai, Go Yamakawa, Mayuki Omatsu, Mio Namikawa, Tomonori Masuda, Yukiko Hiramatsu, Yu Muta, Takahisa Maruno, Yuki Nakanishi, Tatsuaki Tsuruyama, Hiroshi Seno

“…With advancements in 3D culture technology, cholangiocyte organoids (COs) derived from primary tissues or induced pluripotent stem cells (iPSCs) can accurately replicate the structural and functional properties of biliary tissues. …”
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Current Bioengineering Methods for Whole Kidney Regeneration by Shuichiro Yamanaka, Takashi Yokoo

“…Here, we review recent advances in the field of kidney regeneration, including (i) the directed differentiation of induced pluripotent stem cells/embryonic stem cells into kidney cells; (ii) blastocyst decomplementation; (iii) use of a decellularized cadaveric scaffold; (iv) embryonic organ transplantation; and (v) use of a nephrogenic niche for growing xenoembryos for de novo kidney regeneration from stem cells. …”
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Unchain My Heart: Integrins at the Basis of iPSC Cardiomyocyte Differentiation by Rosaria Santoro, Gianluca Lorenzo Perrucci, Aoife Gowran, Giulio Pompilio

“…In particular, mechanotransduction is of growing importance in groundbreaking cellular models such as induced pluripotent stem cells (iPSC), since this process may strongly influence cell fate and, thus, augment the precision of differentiation into specific cell types, e.g., cardiomyocytes. …”
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Protein Kinases and Their Inhibitors in Pluripotent Stem Cell Fate Regulation by Jungwoon Lee, Young-Jun Park, Haiyoung Jung

“…Furthermore, new reprogramming technologies have been developed using kinase modulators, which have provided novel insight of the mechanisms underlying the kinase regulatory networks involved in the generation of induced pluripotent stem cells (iPSCs). In this review, we highlight the recent progress made in defining the roles of protein kinase signaling pathways and their small molecule modulators in regulating the pluripotent states, self-renewal, reprogramming process, and lineage differentiation of PSCs.…”
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Emerging Stem Cell Therapies: Treatment, Safety, and Biology by Joel Sng, Thomas Lufkin

“…The discovery of induced pluripotent stem cells has led to an improved understanding of the underlying epigenetic keys of pluripotency and carcinogenesis. …”
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A synthetic chronogenetic gene circuit for programmed circadian drug delivery by Lara Pferdehirt, Anna R. Damato, Kristin L. Lenz, Maria F. Gonzalez-Aponte, Daniel Palmer, Qing-Jun Meng, Erik D. Herzog, Farshid Guilak

“…Gene circuits were transduced into induced pluripotent stem cells that were tissue-engineered into cartilage constructs. …”
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Current Therapeutic Strategies for Stem Cell-Based Cartilage Regeneration by Yoojun Nam, Yeri Alice Rim, Jennifer Lee, Ji Hyeon Ju

“…We also review the chondrogenic potential of cells according to their origin, including autologous chondrocytes, mesenchymal stem cells, and induced pluripotent stem cells.…”
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Highly efficient construction of monkey blastoid capsules from aged somatic cells by Junmo Wu, Tianao Shao, Zengli Tang, Gaojing Liu, Zhuoyao Li, Yuxi Shi, Yu Kang, Jiawei Zuo, Bo Zhao, Guangyu Hu, Jiaqi Liu, Weizhi Ji, Lei Zhang, Yuyu Niu

“…In this work, we demonstrate the efficient generation of blastoids from induced pluripotent stem cells and somatic cell nuclear transfer embryonic stem cells derived from aged monkeys, achieving an 80% formation efficiency. …”
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