Development of a Cell-Based Functional Assay for the Detection of Clostridium botulinum Neurotoxin Types A and E by Uma Basavanna, Tim Muruvanda, Eric W. Brown, Shashi K. Sharma

“…Here, we describe a cell-based assay utilizing a fluorescence reporter construct expressed in a neuronal cell model to study toxin activity in situ. Our data indicates that the assay can detect as little as 100 pM BoNT/A activity within living cells, and the assay is currently being evaluated for the analysis of BoNT in food matrices. …”
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Application of Artificial Intelligence Models to Predict the Tensile Strength of Glass Fiber-Modified Cemented Backfill Materials during the Mine Backfill Process by Lei Zhu, Wenzhe Gu, Zhicheng Liu, Fengqi Qiu

“…Due to the complex stress environment of in situ stress, mining stress, water pressure, and gas pressure, cemented backfill materials need to have good mechanical properties, and glass fiber is usually used to mix into cemented backfill materials to improve its performance, but there are many problems including complex testing process, high cost, and long time-consuming in the study of mechanical properties of glass fiber-modified cemented backfill materials (GFCBM) by laboratory tests. …”
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Investigation on Inorganic Salts K2TiF6 and KBF4 to Develop Nanoparticles Based TiB2 Reinforcement Aluminium Composites by Vinayagam Mohanavel, K. Ravi Kumar, T. Sathish, Palanivel Velmurugan, Alagar Karthick, M. Ravichandran, Saleh Alfarraj, Hesham S. Almoallim, Shanmugam Sureshkumar, J. Isaac JoshuaRamesh Lalvani

“…In the current research, AA6082 aluminium alloy matrix composites (AAMCs) incorporated with various weight fractions of titanium diboride (0, 3, 6, and 9 wt%) were prepared via an in situ casting technique. The exothermic reaction between inorganic powders like dipotassium hexafluorotitanate (K2TiF6) and potassium tetrafluoroborate (KBF4) in molten Al metal contributes to the development of titanium diboride content. …”
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Experiment Investigation on Stress Characteristics of Grouting Microsteel Pipe Piles with Cement-Soil Wall by Shujuan Yang, Mingyi Zhang, Xiaoyu Bai, Xueying Liu, Chen Zheng

“…The inner force change laws and bearing capacity of microsteel pipe piles were discussed through in situ stress measurement and laboratory antibending tests on three microsteel pipe piles. …”
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Study of GaN Hall Effect Magnetic Sensors by Vlad Marsic, Soroush Faramehr, Isha Maini, David A. J. Moran, Petar Igic

“…The GaN Hall sensors are the latest advancements in non-intrusive active monitoring, designed to be integrated on GaN power transistors to enable in-situ condition monitoring, hence boosting device reliability and life. …”
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DPSCs Protect Architectural Integrity and Alleviate Intervertebral Disc Degeneration by Regulating Nucleus Pulposus Immune Status by Xiwen Dong, Fanqi Hu, Jing Yi, Yuning Zhang, Chao Liu, Panpan Geng, Han Duan, Chu-Tse Wu, Xuesong Zhang, Hua Wang

“…Besides, to evaluate the therapeutic effect of DPSCs in IVD degeneration treatment, DPSCs were injected into a degeneration rat model in situ, with treatment outcome measured by micro-CT and histological analysis. …”
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Electric and Magnetic Properties of Sputter Deposited BiFeO3 Films by N. Siadou, I. Panagiotopoulos, N. Kourkoumelis, T. Bakas, K. Brintakis, A. Lappas

“…Polycrystalline BiFeO3 films have been magnetron sputter deposited at room temperature and subsequently heat-treated ex situ at temperatures between 400 and 700°C. The deposition was done in pure Ar atmosphere, as the use of oxygen-argon mixture was found to lead to nonstoichiometric films due to resputtering effects. …”
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Temperature-Sensitive Gels for Intratumoral Delivery of β-Lapachone: Effect of Cyclodextrins and Ethanol by Marcilio S. S. Cunha-Filho, Carmen Alvarez-Lorenzo, Ramón Martínez-Pacheco, Mariana Landin

“…This work evaluated the potential of Pluronics (varieties F127 and P123) in combination with solubilizing agents to be used as syringeable in situ gelling depots of intratumoral β-lapachone (βLAP). …”
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Physical and Biological Characterizations of Injectable Thermosensitive Poly(N-Isopropylacrylamide)-Collagen I Hydrogels Intended for Meniscus Repair by Chunhai Ke, Xuanyuan Lu, Jiahua Ni, Ruicong Li, Qi Chen, Zhaoxiang Peng

“…There has been an increase in the incidence of meniscus injuries worldwide, as well as growing needs for injectable hydrogels with the potentials to promote in situ meniscal tissue repair. This work developed poly(N-isopropylacrylamide)-collagen I composite hydrogels, with a series of different collagen I contents. …”
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El espacio público en disputa. El caso de la calle de acceso al campus de la Universidad Nacional del Comahue by Romina Valeria Schroeder

“…Con respecto a la metodología, se incluye recopilación de variada información, observación in situ y la elaboración de un sondeo de opinión que hace hincapié, principalmente, en la percepción que la comunidad universitaria tiene acerca de los cambios que se vienen generando. …”
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Estudio cualitativo sobre fisuración debida a la reacción álcali -sílice en el forjado de la represa hidráulica de Jaguari by Camila Zoilo, Jorge Akasaki, Cesar Fioriti, Haroldo de Mayo Bernardes, Flávio Moreira Salles, Adriana Pereira

“…En el presente trabajo se realizó un análisis cualitativo de las grietas que se producen en la losa por la entrada de agua con un studio visual in situ, y los resultados de análisis por ordenador usando el método de elementos finitos. …”
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Intercomparison of Downscaling Techniques for Satellite Soil Moisture Products by Daeun Kim, Heewon Moon, Hyunglok Kim, Jungho Im, Minha Choi

“…This study introduces a new downscaling method based on support vector regression (SVR) that includes the geophysical variables with locational weighting. Regarding the in situ SM, the SVR downscaling method exhibited a smaller root mean square error, from 0.09 to 0.07 m3·m−3, and a larger average correlation coefficient increased, from 0.62 to 0.68, compared to the conventional method. …”
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Prenatal Identification and Molecular Characterization of Two Simultaneous De Novo Interstitial Duplications of Chromosomal Regions 7p22.1p21.1 and 15q24.1 by Sabrina C. Burn, Kali Swift, Maria Palmquist

“…Cytogenetic analysis of cultured amniocytes by conventional chromosome analysis, comparative genomic hybridization, and fluorescence in situ hybridization revealed two interstitial duplications of the chromosomal regions 7p22.1p21.1 and 15q24.1, leading to partial trisomy of 7p and 15q and karyotype 46,XY,dup(7)(p22.1-p21.1),dup (15)(q24.1). …”
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Intratumor heterogeneity: models of malignancy emergence and evolution by R. A. Ivanov, S. A. Lashin

“…Traditional experimental methods involve genomic profiling techniques such as next­generation sequencing and fluorescence in situ hybridization. These techniques enable the identification of somatic mutations, copy number alterations, and structural rearrangements within cancer genomes. …”
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Hybrid breeding boosted molecular genetics in rye by R. Schlegel

“…Only through the development of new methods such as C-banding, in situ DNA hybridization, enzymology and molecular marker techniques achieved an enormous progress. …”
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Spatial transcriptomics of healthy and fibrotic human liver at single-cell resolution by Brianna R. Watson, Biplab Paul, Raza Ur Rahman, Liat Amir-Zilberstein, Åsa Segerstolpe, Eliana T. Epstein, Shane Murphy, Ludwig Geistlinger, Tyrone Lee, Angela Shih, Jacques Deguine, Ramnik J. Xavier, Jeffrey R. Moffitt, Alan C. Mullen

“…Here we apply multiplexed error robust fluorescent in situ hybridization (MERFISH) to map the zonal distribution of hepatocytes, spatially resolve subsets of macrophage and mesenchymal populations, and investigate the relationship between hepatocyte ploidy and gene expression within the healthy human liver. …”
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A New Evaluation Method for Water Blocking Performance of Coal Seam Floor: Model Construction, Case Application, and Water-Preserved Strategy by Baobin Gao, Chuangnan Ren, Shaopeng Song

“…Furthermore, an optimized strategy for coal seam floor water retention mining is proposed: the technology system of water-preserved mining for coal seam floor contains 3 stages and 3 detections, which provides a scientific basis for the in situ protection of high-pressure limestone water from coal seam floor.…”
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Multifeature Extraction of Three-Dimensional Topography of Carbon Steel Specimen during Fatigue Process by Tao Liu, Jingxiong Wu, Jingfa Lei, Xue Wang, Bingqi Zhang, Shu Zhang

“…In order to investigate the variation of three-dimensional metal surface topography during fatigue process, a three-dimensional (3D) topography acquisition platform was built with an in situ tensile tester and a three-dimensional profilometer. …”
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Placental Infection with Different SARS-CoV-2 Variants Leading to Stillbirth: Report of Two Cases by Miriam A. Snow, Medini K. Annavajhala, Sofia Z. Moscovitz, Anne-Catrin Uhlemann, Larisa Debelenko

“…Placental tissues were positive for SARS-CoV-2 by in situ hybridization (ISH) and immunohistochemistry (IHC). …”
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DNA Ploidy and S-Phase Fraction in Carcinoma of the Gallbladder Related to Histopathology, Number of Gallstones and Survival 1 by Ulf Gustafsson, Curt Einarsson, Lennart C. Eriksson, Virgil Gadaleanu, Staffan Sahlin, Bernhard Tribukait

“…S‐phase fraction increased during development from normal tissue to dysplasia, cancer in situ and cancer in diploid cases (p=0.0002), and further at the change from diploid to aneuploid (p=0.004). …”
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