Epidemiology of bacterial biofilms on polyps and normal tissues in a screening colonoscopy cohort by Julia L. Drewes, Samara B. Rifkin, Madison McMann, Sara Glass, Emma Spence, Caroline R. Wensel, Abby L. Geis, Courtney Stevens, Joell J. Gills, Hao Wang, Linda M. Hylind, Gerard Mullin, David Kafonek, David Cromwell, Louis La Luna, Francis M. Giardiello, Cynthia L. Sears

“…Here we evaluated potential procedural and demographic risk factors associated with biofilm status using a cross-sectional observational cohort.Methods Histologically normal colonic biopsies from 2,051 individuals undergoing screening colonoscopy were evaluated for biofilm status using fluorescence in situ hybridization with oligonucleotide probes targeting bacterial 16S rRNA. …”
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Turning the Surface Electronic Effect Over Core‐Shell CoS2─FexCo1‐xS2 Nanooctahedra Toward Electrochemical Water Splitting in the Alkaline Medium by Lian‐Ming Lyu, Yu‐Chung Chang, Han‐Jung Li, Pei‐En Wang, Ruei‐Hung Juang, Ming‐Yen Lu, Cheng‐Shiuan Li, Chun‐Hong Kuo

“…From the confirmations by in‐situ X‐ray spectroscopies in reaction kinetics, the Co sites of Fe0.9Co0.1S2(111) on the core‐shell nanooctahedra exhibited much higher activities than those of CoS2(111) under the applied potentials for OER and HER, which reflected the electronic benefits from the existing Fe neighbors.…”
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Construction of complex three-dimensional vascularized liver tissue model in vitro based on a biphasic cell-laden embedding medium by Weikang Lv, Haoran Yu, Abdellah Aazmi, Tuya Naren, Wanli Cheng, Mengfei Yu, Zhen Wang, Xiaobin Xu, Huayong Yang, Liang Ma

“…By adjusting the printing speed, we controlled the filament diameter, achieving a range from 250 μ m to 1000 μ m, and ensuring precise control over ink deposition locations and filament shapes. Using the in situ endothelialization method, we constructed complex vascular structures and ensured close adhesion between hepatocytes and endothelial cells. …”
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Long non-coding RNA Malat1 modulates CXCR4 expression to regulate the interaction between induced neural stem cells and microglia following closed head injury by Qin Dong, Pengyu Chen, Wenqiao Qiu, Zhijun Yang, Yanteng Li, Yuhui Zhou, Lili Guo, Dan Zou, Ruxiang Xu, Mou Gao

“…Subsequently, we predicted the target genes and performed gain- and loss-of-functional studies, dualluciferase reporter, RNA immunoprecipitation, biotin-coupled miRNA pulldown, fluorescence in situ hybridization and cell transplantation assays to further elucidate the mechanism underlying the immunoregulatory effects of iNSCs. …”
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Novel combination strategy of high intensity focused ultrasound (HIFU) and checkpoint blockade boosted by bioinspired and oxygen-supplied nanoprobe for multimodal imaging-guided ca... by Ying Zhou, Chen Cheng, Qi Wang, Min Zheng, Pan Li, Yang Zhou, Yuting Cao, Li Wan, Xun Guo, Xiaohong Lin, Rui Tang, Hongye He, Nianhong Wu, Qiaoqi Chen, Yaqin Hu, Xialin Xiong, Faqi Li

“…Flow cytometry and immunofluorescence were used to clarify the potential mechanism of HIFU+M@P-SOP and their role in anti-programmed death ligand-1 (PD-L1) therapy.Results Guided by photoacoustic/MR/ultrasound (US) multimodal imaging, M@P-SOP was abundantly enriched in tumor, which greatly enhanced HIFU’s killing of tumor tissue in situ, induced stronger tumor immunogenic cell death, stimulated dendritic cell maturation and activated CD8+ T cells. …”
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High-pressure structural and electrical properties of pyrochlore: Enhanced octahedral distortions and electrical resistance of La2Sn2O7 by Shufan Jia, H. Saqib, Daniel Errandonea, Yuxin Hu, Bin Zeng, Xiangyu Chen, Alfonso Munoz, Silvana Radescu, Andres Mujica, Yongsheng Zhao, S. Rahman, Ning Dai

“…The effect of high pressure on the structural and electrical properties of La2Sn2O7 have been studied using Raman spectroscopy, in situ angle dispersive X-ray diffraction (ADXRD) and alternating current (AC) impedance spectroscopy. …”
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Injectable Nanocomposite Hydrogel for Accelerating Diabetic Wound Healing Through Inflammatory Microenvironment Regulation by Chen Y, Li Y, Song H, Liu X, Zhang H, Jiang J, Liu H, Zhuo R, Cheng G, Fang J, Xu L, Qi Y, Sun D

“…Additionally, a full-thickness skin defect model of diabetic rat was established to investigate the hydrogel’s multifaceted efficacy in facilitating wound repair, mitigating inflammatory responses, and fostering angiogenesis.Results: The thermosensitive MCC@ZIF-8@Cur hydrogel possess the attribute of being injectable and capable of in situ formation (gelation temperature of ≥ 28 °C), thereby establishing an effective physical barrier for a multitude of irregular wound profiles. …”
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Assessment of atmospheric correction methods in MSI imagery for deriving bathymetry and substrates of shallow-water coral reefs by Wei Huang, Wei Huang, Wei Huang, Wei Huang, Jun Zhao, Mingjie Li, Mingjie Li, Mingjie Li, Quansheng Lou, Quansheng Lou, Nanyang Yan, Nanyang Yan, Nanyang Yan, Shaojie Sun

“…Polymer-derived remote sensing reflectance showed excellent agreement with in situ measurements, with R2 > 0.95 in 3-hour matches and R2 > 0.65 in 6-hour matches in visible bands (443nm-665nm). …”
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Analysis of different therapeutic schemes combining cyclophosphamide and doublestranded DNA preparation for eradication of Krebs-2 primary ascites in mice by E. A. Potter, E. V. Dolgova, A. M. Minkevich, V. P. Nikolin, N. A. Popova, Ya. R. Efremov, S. I. Baiborodin, V. A. Rogachev, A. S. Proskurina, A. V. Kozel, O. S. Taranov, V. V. Omigov, E. I. Vereschagin, D. B. Petrov, A. A. Ostanin, E. R. Chernykh, N. A. Kolchanov, S. S. Bogachev

“…In the present paper, we report on the series of experiments where multiple regimens of CP and dsDNA injections were tested for targeting the ascites form of murine Krebs-2 cancer in situ. We show that combining CP with cross-linked human and salmon dsDNA results in a synergistic toxicity for ascites-bearing mice, an observation supported by the histopathology analysis of organs and tissues of experimental animals. …”
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Single‐cell RNA‐sequencing and spatial transcriptomic analysis reveal a distinct population of APOE− cells yielding pathological lymph node metastasis in papillary thyroid cancer... by Guohui Xiao, Rongli Xie, Jianhua Gu, Yishu Huang, Min Ding, Dongjie Shen, Jiqi Yan, Jianming Yuan, Qiong Yang, Wen He, Siyu Xiao, Haizhen Chen, Dan Xu, Jian Wu, Jian Fei

“…A machine‐learning bioinformatics model based on scRNA‐seq data of in‐situ thyroid cancer tissue was established to predict lymph node metastasis.…”
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Identification of glutamine as a potential therapeutic target in dry eye disease by Xiaoniao Chen, Chuyue Zhang, Fei Peng, Lingling Wu, Deyi Zhuo, Liqiang Wang, Min Zhang, Zhaohui Li, Lei Tian, Ying Jie, Yifei Huang, Xinji Yang, Xiaoqi Li, Fengyang Lei, Yu Cheng

“…Our study demonstrated that co-treatment with mesenchymal stem cells (MSCs) and thymosin beta-4 (Tβ4) yielded the best therapeutic outcome against dry eye, surpassing monotherapy outcomes. In situ metabolomics through matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) revealed increased glutamine levels in cornea following MSC + Tβ4 combined therapy. …”
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Development of the early fetal human thalamus: from a protomap to emergent thalamic nuclei by Maznah Alhesain, Ayman Alzu’bi, Ayman Alzu’bi, Ayman Alzu’bi, Niveditha Sankar, Niveditha Sankar, Charles Smith, Charles Smith, Janet Kerwin, Ross Laws, Susan Lindsay, Gavin J. Clowry

“…Higher order thalamic nuclei, relaying information between cortical regions and important in higher cognitive function, are greatly expanded.MethodsThis study mapped the emergence of thalamic nuclei in human fetal development (8–16 post conceptional weeks; PCW) by revealing gene expression patterns using in situ hybridization and immunohistochemistry for previously established thalamic development markers.ResultsIn the proliferative thalamic ventricular zone, OLIG3 and NR2F1 immunoreactivity marked the extent of the thalamus, whereas PAX6 and NR2F2 were expressed in gradients, suggesting an early protomap. …”
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Three-dimensional reconstruction and connectivity analysis of REV-size organic matter in shales by Dongchen Liu, Dongchen Liu, Dongchen Liu, Xuefeng Yang, Xuefeng Yang, Shengxian Zhao, Shengxian Zhao, Deliang Zhang, Deliang Zhang, Shan Huang, Shan Huang, Ning Zhu, Ning Zhu, Jianqi Rong, Bowen Shi, Zhiwei Wang, Chao-Zhong Qin

“…Based on the case studies of three in-situ shale samples with different OM contents, we show that at the REV size the three types of OM, as a whole, can form conducting pathways throughout the domains, but each type of OM is disconnected. …”
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Proteomics unveil candidate biomarkers and pathogenesis of subacute thyroiditis by Litong Ran, Xiufei Liu, Yongfeng Tian, Jiaran Zhu, Zhengyuan Gong, Qiao Qiao, Xin Jiang, Yuren Wang, Guojun Yang, Hongting Zheng, Yi Zheng, Hua Qu

“…Here, we profiled thyroid in situ protein alterations in fine-needle aspiration biopsy samples from SAT patients using proteomic analysis and uncovered 57 differentially abundant proteins. …”
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Deciphering the Differences Between Epstein–Barr Virus‐Associated and Negative Gastric Cancer in the Prospect of CDKN2A Genomic Alterations and Lymphoid Infiltration by Fuda Xie, Bonan Chen, Yang Lyu, Peiyao Yu, Canbin Fang, Kam Tong Leung, Shouyu Wang, Dazhi Xu, Jun Yu, Kwok Wai Lo, Ka Fai To, Wei Kang

“…Methods GC samples were stained by immunohistochemistry, fluorescent and EBV‐encoded small RNA in situ hybridization to identify CD8 expression, CDKN2A genomic alteration, and EBV existence. …”
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Leveraging AI to automate detection and quantification of extrachromosomal DNA to decode drug responses by Kohen Goble, Aarav Mehta, Damien Guilbaud, Jacob Fessler, Jingting Chen, William Nenad, William Nenad, Christina G. Ford, Oliver Cope, Darby Cheng, William Dennis, Nithya Gurumurthy, Yue Wang, Kriti Shukla, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk, Elizabeth Brunk

“…Extrachromosomal DNA (ecDNA), in contrast, represents a rapid, reversible, and predictable DNA alteration critical for cancer’s adaptive response.MethodsIn this study, we developed a novel post-processing pipeline for automated detection and quantification of ecDNA in metaphase Fluorescence in situ Hybridization (FISH) images, leveraging the Microscopy Image Analyzer (MIA) tool. …”
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Transcriptomic signatures of cold acclimated adipocytes reveal CXCL12 as a Brown autocrine and paracrine chemokine by Marina Agueda-Oyarzabal, Marie S. Isidor, Kaja Plucińska, Lars R. Ingerslev, Oksana Dmytriyeva, Patricia S.S. Petersen, Sara Laftih, Axel B. Pontoppidan, Jo B. Henningsen, Kaja Rupar, Erin L. Brown, Thue W. Schwartz, Romain Barrès, Zachary Gerhart-Hines, Camilla C. Schéele, Brice Emanuelli

“…Cxcl12 mRNA expression analysis by RT-qPCR and fluorescence in situ hybridization specified Cxcl12 distribution in various cell types, and indicated its enrichment in cold-acclimated brown adipocytes. …”
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A compilation of surface inherent optical properties and phytoplankton pigment concentrations from the Atlantic Meridional Transect by T. M. Jordan, G. Dall'Olmo, G. Tilstone, R. J. W. Brewin, F. Nencioli, R. Airs, C. S. Thomas, L. Schlüter

“…<p>In situ measurements of particulate inherent optical properties (IOPs) – absorption (<span class="inline-formula"><i>a</i>_p(<i>λ</i>)</span>), scattering (<span class="inline-formula"><i>b</i>_p(<i>λ</i>)</span>), and beam attenuation (<span class="inline-formula"><i>c</i>_p(<i>λ</i>)</span>) – are crucial for the development of optical algorithms that retrieve biogeochemical quantities such as chlorophyll <span class="inline-formula"><i>a</i></span>, particulate organic carbon (POC), and total suspended matter (TSM). …”
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Tunning valence state of cobalt centers in Cu/Co-CoO1-x for significantly boosting water-gas shift reaction by Xing-Chi Li, Jun-Hao Wang, Tao-Tao Huang, Yang Hu, Xin Li, De-Jiu Wang, Wei-Wei Wang, Kai Xu, Chun-Jiang Jia, Hao Dong, Guangshe Li, Chen Li, Ya-Wen Zhang

“…A synergistic mechanism is proposed that Coδ+ species can be easily reduced by CO adsorbed on Cu and Co0 can be oxidized by H2O. Systematic in situ characterization results reveal that the addition of Cu can regulate the redox properties of Co species and thus modulate the adsorption properties of catalysts. …”
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Pressure‐Enhanced Superconductivity and Structural Phase Transition in Layered Sn4P3 by Hao Ding, Jingyu Hou, Kun Zhai, Xin Gao, Junquan Huang, Feng Ke, Bingchao Yang, Congpu Mu, Fusheng Wen, Jianyong Xiang, Bochong Wang, Tianyu Xue, Anmin Nie, Xiaobing Liu, Lin Wang, Xiang‐Feng Zhou, Zhongyuan Liu

“…Comprehensive measurements using in situ synchrotron X‐Ray diffraction and Raman spectroscopy reveal a structural phase transition with mild distortion of SnP3 building blocks and interlayer shrinkage under high pressure. …”
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