Toward autism spectrum disorders and Williams-Beuren syndrome co-occurrence condition in Tunisian patients: Genetic insights by Rim Khelifi, Afef Jelloul, Houda Ajmi, Wafa Slimani, Sarra Dimassi, Khouloud Rjiba, Manel Dardour, Moez Gribaa, Ali Saad, Soumaya Mougou-Zerelli

“…A comprehensive evaluation using karyotyping, fluorescence in situ hybridization (FISH), and array comparative genomic hybridization (array-CGH) was performed.ResultsAll patients were clinically diagnosed and confirmed to have WBS through karyotyping and FISH analysis. …”
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Investigation of Woody Species Structure and Regeneration Status in the Central Rift Valley, Sidama Regional State, Ethiopia by Assegid Assefa, Tamrat Bekele, Sebsebe Demissew, Tesfaye Abebe

“…Protected areas are the most commonly used tool for in situ conservation of biodiversity. Selective removal of species proposed by the local communities living surrounding the national park and grazing pressure negatively affect the composition, structure, and regeneration of woody species. …”
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Development, optimization and application of a universal fluorescence multiplex PCR-based assay to detect BCOR genetic alterations in pediatric tumors by Meng Zhang, Xingfeng Yao, Nan Zhang, Yongbo Yu, Chao Jia, Xiaoxing Guan, Wenjian Xu, Xin Ni, Yongli Guo, Lejian He

“…However, gene fusions are usually detected through reverse transcription-polymerase chain reaction (RT-PCR) or fluorescence in situ hybridization. Methods that analyze these variants simultaneously in a sensitive and convenient manner are lacking in clinical practice. …”
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Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse by Sara Carmela Credendino, Nicole Lewin, Miriane de Oliveira, Swaraj Basu, Barbara D’Andrea, Elena Amendola, Luigi Di Guida, Antonio Nardone, Remo Sanges, Mario De Felice, Gabriella De Vita

“…We examine the whole expression level of Klhl14-AS in a panel of adult mouse tissues, showing that it is expressed in the thyroid, lung, kidney, testis, ovary, brain, and spleen, although at different levels. In situ hybridization analysis reveals that, in the context of each organ, Klhl14-AS shows a cell type-specific expression. …”
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The necroptosis-related lncRNA ENSG00000253385.1 promotes the progression of esophageal squamous cell carcinoma by targeting the miR-16-2-3p/VDAC1 axis by Xiaoyang Duan, Jian Shi, Ran Hou, Yajie Huang, Chunyan Wang, Huazhen Du

“…We detected the location of the lncRNA ENSG00000253385.1 in ESCC cells by fluorescence in situ hybridization (FISH). FISH and quantitative real-time polymerase chain reaction (qRT‒PCR) were used to detect gene expression in ESCC tissues and cells. …”
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The benefits and trade-offs of multi-variable calibration of the WaterGAP global hydrological model (WGHM) in the Ganges and Brahmaputra basins by H. M. M. Hasan, P. Döll, P. Döll, S.-M. Hosseini-Moghari, F. Papa, A. Güntner, A. Güntner

“…The selected model parameters, determined through a multi-variable, multi-signature sensitivity analysis, were estimated using up to four types of observations: in situ streamflow (<span class="inline-formula"><i>Q</i></span>), GRACE and GRACE Follow-On terrestrial water storage anomaly (TWSA), LandFlux evapotranspiration (ET), and surface water storage anomaly (SWSA) derived from multi-satellite observations. …”
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Systemic Overexpression of GDF5 in Adipocytes but Not Hepatocytes Alleviates High-Fat Diet-Induced Nonalcoholic Fatty Liver in Mice by Yan Yang, Wenting Zhang, Xiaohui Wu, Jing Wu, Chengjun Sun, Feihong Luo, Zhou Pei

“…Compared to that of cells in the FAA-free control group, LO2 cells with in situ overexpression of GDF5 developed lipid droplets after FFA treatment; the levels of triglycerides, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were significantly increased in both the GDF5 lentivirus and control lentivirus groups compared with those of the FAA-free group. …”
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Mangrove biomass productivity and sediment carbon storage assessment at selected sites in Mauritius: the effect of tidal inundation, forest age and mineral availability by M D D Doodee, S D D V Rughooputh, S Jawaheer

“…Using 25m x 25m quadrats and a simple random sampling technique, plant height and diameter at breast height (DBH) were measured in situ with the GLOBE Observer application (The Globe Program, NASA-sponsored) and a measuring tape, respectively. …”
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A gut-derived Streptococcus salivarius produces the novel nisin variant designated nisin G and inhibits Fusobacterium nucleatum in a model of the human distal colon microbiome by Garreth W. Lawrence, Enriqueta Garcia-Gutierrez, A. Kate O’Mahony, Calum J. Walsh, Paula M. O'Connor, Máire Begley, Caitriona M. Guinane, Paul D. Cotter

“…Bacteriocin-producing probiotic bacteria demonstrate the potential to target disease-associated taxa in situ in the gut. A gut-derived strain Streptococcus salivarius DPC6487 was found to demonstrate anti-F. nucleatum activity, which was attributable to a gene encoding a novel nisin variant designated nisin G. …”
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Detecting gene copy number alterations by Oncomine Comprehensive genomic profiling in a comparative study on FFPE tumor samples by Fabio Bozzi, Elena Conca, Marco Silvestri, Gianpaolo Dagrada, Alice Ardore, Donata Penso, Daniele Lorenzini, Chiara Costanza Volpi, Desirè Viola Trupia, Adele Busico, Iolanda Capone, Federica Perrone, Elena Tamborini, Andrea Vingiani, Luca Agnelli, Giancarlo Pruneri

“…The CNAs recognition in formalin fixed paraffin embedded (FFPE) material for diagnostic purposes has relied for years mainly on fluorescence in situ hybridization. The introduction of other procedures, such as Next-Generation Sequencing has dramatically improved CNAs discovery at genome-wide level. …”
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Rainwater and fog harvesting from solar panels by I. Alazzam, K. Shatanawi, R. Al-Weshah

“…This study aimed to evaluate the quantity of rainwater and fog collected through the utilization of solar panels, while also conducting a feasibility analysis on the economic and environmental aspects of employing solar panels for rainwater and fog harvesting in a solar farm situated in Jordan.METHODS: In the present study, an in-situ experiment is conducted to investigate rainwater and fog harvesting from solar panels' surfaces that are widely spread in Jordan. …”
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Surgical Injury and Ischemia Prime the Adipose Stromal Vascular Fraction and Increase Angiogenic Capacity in a Mouse Limb Ischemia Model by Satoko Kishimoto, Ken-ichi Inoue, Ryoichi Sohma, Shigeru Toyoda, Masashi Sakuma, Teruo Inoue, Ken-ichiro Yoshida

“…HIF-1 alpha was stabilized in the primed cutaneous fat in situ, and VEGF-A synthesis of the primed SVFs was on a peak on 5 days after priming. …”
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The role of manganese in CoMnOx catalysts for selective long-chain hydrocarbon production via Fischer-Tropsch synthesis by Hao Chen, Zan Lian, Xiao Zhao, Jiawei Wan, Priscilla F. Pieters, Judit Oliver-Meseguer, Ji Yang, Elzbieta Pach, Sophie Carenco, Laureline Treps, Nikos Liakakos, Yu Shan, Virginia Altoe, Ed Wong, Zengqing Zhuo, Feipeng Yang, Ji Su, Jinghua Guo, Monika Blum, Saul H. Lapidus, Adrian Hunt, Iradwikanari Waluyo, Hirohito Ogasawara, Haimei Zheng, Peidong Yang, Alexis T. Bell, Núria López, Miquel Salmeron

“…Here we present an experimental and theoretical study using model catalysts consisting of crystalline CoMnOx nanoparticles and thin films, where Co and Mn are mixed at the sub-nm scale. Employing TEM and in-situ X-ray spectroscopies (XRD, APXPS, and XAS), we determine the catalyst’s atomic structure, chemical state, reactive species, and their evolution under FTS conditions. …”
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Microbes and metabolites of a plant-parasite interaction: Deciphering the ecology of Tetrastigma host choice in the world’s largest parasitic flower, Rafflesia by Jeanmaire Molina, Roche C. de Guzman, Rinat Abzalimov, Wenkai Huang, Anusha Guruprasad, Ronniel Pedales, Adhityo Wicaksono, Destiny Davis, John Rey Callado, Hans Bänziger, Piyakaset Suksathan, William Eaton, Pride Yin, Marco Bürger, Mick Erickson, Stephen Jones, James Adams, Susan Pell

“…In identifying essential microbial and chemical conditions that could improve propagation techniques, this research has practical applications in ex situ conservation efforts, aiding in the rescue of the world’s largest flowers from the brink of extinction.…”
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Vingt ans de pédagogie au Parc zoologique et botanique de Mulhouse : la place des Primates by Corinne Di Trani‑Zimmermann

“…La nécessité de les préserver est abordée spécifiquement lors des projets scolaires ou des visites pour le grand public illustrant les actions mises en place dans les programmes ex et in situ. Le Parc zoologique et botanique a essayé de rendre cette sensibilisation plus concrète en mobilisant les visiteurs autour d’actions engageantes, que ce soit par le biais d’échanges pédagogiques entre élèves ou de partage d’expérience lors des évènements festifs annuels au profit des espèces rares et des milieux naturels.…”
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The Modulation of Laser Refractive Surgery on Sensory Eye Dominance of Anisometropia by Hongting Liu, Qi Chen, Fangfang Lan, Yan Luo, Enwei Lin, Wuqiang Luo, Ming Kong, Jiangxia Wang, Fengju Zhang

“…The second part of the study included 40 subjects with nonanisometropic myopia and 40 subjects with anisometropic myopia who received the femtosecond laser-assisted in situ keratomileusis (Fs-LASIK). A comprehensive ophthalmologic evaluation was performed with particular attention to sensory eye dominance preoperatively and one-week and one-month postoperatively. …”
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Organotin(IV) trifluoromethanesulfonate chemistry: isolation and characterization of novel 1,10-phenanthroline-based derivatives by Cattey, Hélène, Plasseraud, Laurent

“…Monitoring by 119Sn{1H} NMR spectroscopy in CD3CN solution of the reaction proceeding with successive addition of phen revealed the in situ formation of three organotin(IV) trifluoromethanesulfonate intermediate species. …”
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Achieving equiaxed and lamellar TiAl alloys via cold-cathode electron beam additive manufacturing with dual-wire synergistic control by Yilei Shi, Shuili Gong, Haiying Xu, Wei Chen, Zhuang Wang, Guang Yang, Xin Liu, Bojin Qi

“…The characteristics of the cold-cathode electron beam heat source were used to manufacture low-cost in-situ alloyed TiAl alloys through wire-fed electron beam additive manufacturing. …”
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Enhanced photocatalytic degradation of Rhodamine B using polyaniline-coated XTiO3(X = Co, Ni) nanocomposites by Mariyem Abouri, Abdellah Benzaouak, Mohamed Elouardi, Lahcen El Hamdaoui, Fatima Zaaboul, Khalil Azzaoui, Belkheir Hammouti, Rachid Sabbahi, Shehdeh Jodeh, Mohammed Alaoui El Belghiti, Adnane El Hamidi

“…Abstract In this study, novel polyaniline-coated perovskite nanocomposites (PANI@CoTiO3 and PANI@NiTiO3) were synthesized using an in situ oxidative polymerization method and evaluated for the photocatalytic degradation of Rhodamine B (RhB) a persistent organic pollutant. …”
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Gas Hydrate Accumulation and Occurrence Associated with Cold Seep Systems in the Northern South China Sea: An Overview by Wei Zhang, Jinqiang Liang, Qianyong Liang, Jiangong Wei, Zhifeng Wan, Junxi Feng, Wei Huang, Jing Zhao, Miaomiao Meng, Wei Deng, Chongmin Chen

“…Based on high-resolution seismic data, subbottom profiles, in situ submergence observations, deep drilling and coring, and hydrate gas geochemical analyses, the geological and geophysical characteristics of these cold seep systems and their associated gas hydrate accumulations in the Qiongdongnan Basin, the Shenhu area, the Dongsha area, and the Taixinan Basin have been investigated. …”
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