Reversible multivalent carrier redox exceeding intercalation capacity boundary by Yuanhe Sun, Rui Qi, Qi Lei, Wei Zhang, Haitao Li, Mengru Lin, Hao Shi, Jianrong Zeng, Wen Wen, Yi Gao, Xiaolong Li, Chunyi Zhi, Daming Zhu

“…Operando X-ray absorption spectroscopy, operando synchrotron X-ray diffraction and composite ex situ characterization jointly demonstrated that intercalated divalent copper is preferentially involved in redox afforded extra electron transfer to form reversible monovalent copper pillars, thus not only ensuring stable topological de/intercalation with high capacity but also sustaining fast migration kinetic paths through reconfigurable pillar effects. …”
Get full text

Evaluation of X-Inactivation Status and Cytogenetic Stability of Human Dermal Fibroblasts after Long-Term Culture by Zhi-Gang Xue, Zhan-Ping Shi, Juan Dong, Ting-Ting Liao, Yan-Peng Wang, Xue-Ping Sun, Zheng-Jie Yan, Xiao-Qiao Qian, Yu-Gui Cui, Juan Chen, Jia-Yin Liu, Guoping Fan

“…Consistently, DNA fluorescent in situ hybridization (FISH) identified 3-4 copies of X chromosomes in one nucleus in this subset of cells with two inactive Xs. …”
Get full text

An Enhanced Three-Band Algorithm for Retrieving Phycocyanin Concentration from Satellite Data in Plateau Inland Lake by Dajun Yuan, Bo-Hui Tang, Dong Wang, Junyi Chen, Zhen Zhang, Zhitao Fu

“…Since the PC absorption feature at 620 nm is prone to interference from non-PC pigments, the algorithm incorporates the quantification of the absorption coefficient of non-PC pigments at 620 nm (<inline-formula><tex-math notation="LaTeX">${{{\bm{a}}}_{{\bm{ph}} - {\bm{pc}}}}( {620} )$</tex-math></inline-formula>) to improve the accurate of estimating <inline-formula><tex-math notation="LaTeX">${{{\bm{a}}}_{{\bm{pc}}}}( {620} )$</tex-math></inline-formula>. Using in situ observation data from Erhai Lake as an example, the results show that ETBA significantly outperforms the original three-band algorithm in mitigating interference from non-PC pigments. …”
Get full text

New insights into interspecies relationships, chromosomal evolution, and hybrid identification in the Lycoris Herb. by Yue Zhang, Shujun Zhou, Yu Chen, Pengchong Zhang, Yongchun Zhang, Junhuo Cai, Zixuan Nie, Lu Zhang

“…This study utilizes karyotype structure, genome size, and fluorescent in situ hybridization (FISH) technology to explore the chromosomal evolution and hybrid identification of Lycoris employing three approaches at the cytogenetic level. …”
Get full text

Study on the protective effect of naringin-regulated lncRNA GAS5 against mitochondrial apoptosis in osteoarthritic chondrocytes by LIN Qin, ZHU Yingkai, LIN Yanming, LAN Shujie, CHEN Changxing, FU Changlong

“…In vitro experiments, Lenti-lncRNA GAS5 was used to infect sodium nitroprusside (SNP)-induced chondrocytes, and the expression of lncRNA GAS5 was detected by fluorescence in situ hybridization in rat chondrocytes; after silencing of lncRNA GAS5, the expression level of lncRNA GAS5 in rat chondrocytes was detected by Real-time PCR; Western blot detected the protein levels of Caspase-3, Bax, MMP-13 and Cyt-C.Results① Pathological changes of cartilage tissue: The articular cartilage matrix in naringenin group was relatively complete, the surface was relatively smooth, the structure was clear, the tide line was more complete, the number of chondrocytes was more full, the arrangement was more orderly, the cartilage staining was more uniform, and the structure was significantly restored than that in the model group. ② lncRNA GAS5 expression in cartilage tissue: Compared with model group, lncRNA GAS5 expression in naringin group was decreased (<italic>P</italic>&lt;0.01). ③ Expression of proteins related to mitochondrial apoptosis in cartilage tissues: Compared with model group, the expressions of Caspase-3, Bax, MMP-13 and Cyt-C in cartilage tissues of naringin group were decreased (<italic>P</italic>&lt;0.01). ④ Fluorescence expression of lncRNA GAS5 in chondrocytes: FISH results showed that the fluorescence expression of lncRNA GAS5 was decreased after naringin treatment of SNP-induced chondrocytes (<italic>P</italic>&lt;0.01). ⑤ The protein expressions of Caspase-3, Bax, MMP-13 and Cyt-C in chondrocytes: Compared with the model group, the protein expressions of Caspase-3, Bax, MMP-13 and Cyt-C in naringin group were significantly decreased (<italic>P</italic>&lt; 0.01). …”
Get full text

The Clinicopathological Features and Prognostic Significance of HER2-Low in Early Breast Tumors Patients Prognostic Comparison of HER-Low and HER2-Negative Breast Cancer Stratified... by Sanaa Gamrani, Laila Akhouayri, Sara Boukansa, Mehdi Karkouri, Hinde El Fatemi

“…A new nomenclature has been proposed for HER2 1+ and HER2 2+ tumors that are confirmed negative according to fluorescence in situ hybridization (FISH). These tumors are now referred to as HER2-low, and it is thought that they may represent a distinct subtype of breast cancer that warrants further investigation. …”
Get full text

Detecting glacial lake water quality indicators from RGB surveillance images via deep learning by Zijian Lu, Xueyan Zhu, Jinfeng Li, Mingyue Li, Jie Wang, Wenqiang Wang, Yili Zheng, Qianggong Zhang

“…In this study, we synchronized surveillance monitoring with in-situ water quality measurements at a typical high-altitude glacial lake on the Qinghai-Tibet Plateau. …”
Get full text

Formation and fate of freshwater on an ice floe in the Central Arctic by M. M. Smith, N. Fuchs, E. Salganik, D. K. Perovich, I. Raphael, M. A. Granskog, K. Schulz, M. D. Shupe, M. D. Shupe, M. Webster

“…Additionally, the contribution from stored precipitation (snowmelt) outweighs by 5 times the input from in situ summer precipitation (rain). The magnitude and rate of local meltwater production are remarkably similar to those observed on the prior Surface Heat Budget of the Arctic Ocean (SHEBA) campaign, where the cumulative summer freshwater production totaled around 1 m during both. …”
Get full text

Composites Based on Poly(ortho-toluidine) and WS₂ Sheets for Applications in the Supercapacitor Field by Teodora Burlanescu, Ion Smaranda, Andreea Androne, Cristina Stefania Florica, Madalina Cercel, Mirela Paraschiv, Adelina Udrescu, Adam Lőrinczi, Petru Palade, Andrei Galatanu, Catalin Negrila, Elena Matei, Monica Dinescu, Radu Cercel, Mihaela Baibarac

“…In this work, three methods for the synthesis of composites based on poly(ortho-toluidine) (POT) and WS₂ are reported: (a) the solid-state interaction (SSI) of POT with WS₂ nanoparticles (NPs); (b) the in situ chemical polymerization (ICP) of ortho-toluidine (OT); and (c) the electrochemical polymerization (ECP) of OT. …”
Get full text

A method to estimate the water storage of on-farm reservoirs by detecting slope gradients based on multi-spectral drone data by Yixuan Wang, Nana Yan, Weiwei Zhu, Zonghan Ma, Bingfang Wu

“…The derived water storage agrees well with in situ observation (R2: 0.99) using slope gradients, reaching an overall accuracy of 95.2 %, with a root mean square error (RMSE) and a mean absolute error (MAE) of 2785 m3 and 1820 m3, respectively. …”
Get full text

Ultrafast Sintering of Dense Li7La3Zr2O12 Membranes for Li Metal All‐Solid‐State Batteries by Faruk Okur, Huanyu Zhang, Julian F. Baumgärtner, Jaka Sivavec, Matthias Klimpel, Gregor Paul Wasser, Romain Dubey, Lars P.H. Jeurgens, Dmitry Chernyshov, Wouter vanBeek, Kostiantyn V. Kravchyk, Maksym V. Kovalenko

“…The chemical and structural evolution of LLZO membranes during the UFS process is characterized through in situ synchrotron X‐ray diffraction and thermogravimetric analysis‐mass spectrometry, complemented by an in‐depth investigation of surface chemistry using X‐ray photoelectron spectroscopy. …”
Get full text

Unveiling the nexus between irradiation and phase reconstruction in tin-lead perovskite solar cells by Wenbo Li, Zhe Li, Shun Zhou, Yanzhuo Gou, Guang Li, Jinghao Li, Cheng Wang, Yan Zeng, Jiakai Yan, Yan Li, Wei Dai, Yaoguang Rong, Weijun Ke, Ti Wang, Hongxing Xu

“…In this study, we investigate the long-term stability problem of tin-lead perovskites under irradiation, counterintuitively discovering an irreversible phase reconstruction process. In-situ photoluminescence spectroscopy is used to monitor the reconstruction process, which involves the reaction of oxygen with photoexcited electrons to form superoxide. …”
Get full text

Feline Papillomatosis by Herman Egberink, Katrin Hartmann, Ralf Mueller, Maria Grazia Pennisi, Sándor Belák, Séverine Tasker, Karin Möstl, Diane D. Addie, Corine Boucraut-Baralon, Tadeusz Frymus, Regina Hofmann-Lehmann, Fulvio Marsilio, Etienne Thiry, Uwe Truyen, Margaret J. Hosie

“…Diagnosis is supported by the histological detection of PV-induced cell changes and intralesional detection of viral antigen (immunostaining) or viral DNA (in situ hybridization). Immunostaining of p16CDKN2A protein (p16) can be performed as a proxy marker for PV-induced neoplasms. …”
Get full text

Preclinical assessment of splicing modulation therapy for ABCA4 variant c.768G>T in Stargardt disease by Dyah W. Karjosukarso, Femke Bukkems, Lonneke Duijkers, Tomasz Z. Tomkiewicz, Julia Kiefmann, Andrei Sarlea, Sander Bervoets, Irene Vázquez-Domínguez, Laurie L. Molday, Robert S. Molday, Mihai G. Netea, Carel B. Hoyng, Alejandro Garanto, Rob W. J. Collin

“…Results Testing of these AONs in patient-derived photoreceptor precursor cells and retinal organoids allow the selection of a lead candidate AON (A7 21-mer) that rescues on average 52% and 50% expression of wild-type ABCA4 transcript and protein, respectively. In situ hybridization and probe-based ELISA demonstrate its distribution and stability in vitro and in vivo. …”
Get full text

Developing a method for root-zone soil moisture monitoring at the field scale using remote sensing and simulation modeling by Hamideh Noory, Morteza Khoshsima, Atsushi Tsunekawa, Mitsuru Tsubo, Nigussie Haregeweyn, Salar Pashapour

“…Because of the high cost and difficulties of operation and maintenance of in situ instrumentation over irrigated fields, fine-scale monitoring of soil moisture (SM) based on remote sensing and a simulation model may be a practical way to inform irrigation practices. …”
Get full text

Analysis of MLKL, RIP1 and RIP3 Immunostaining Markers in Human Liver Tissue from Fatal Yellow Fever Cases: Insights into Necroptosis by Vanessa do Socorro Cabral Miranda, Luiz Fabio Magno Falcão, Hellen Thais Fuzii, Marcos Luiz Gaia Carvalho, Jeferson da Costa Lopes, Arnaldo Jorge Martins Filho, Ana Cecilia Ribeiro Cruz, Raimunda do Socorro da Silva Azevedo, Jorge Rodrigues de Sousa, Mayumi Duarte Wakimoto, Pedro Fernando da Costa Vasconcelos, Juarez Antônio Simões Quaresma

“…The cases underwent histopathological analysis, followed by tissue immunostaining with the immunohistochemical method of streptavidin–biotin peroxidase. Using the in situ method, we evaluated the centrilobular zone (Z3), midzonal zone (Z2), periportal zone and portal tract (PT) of human liver parenchyma with markers for necroptosis, RIPK1, RIPK3 and MLKL. …”
Get full text

Magnetocaloric Effect in 3D Gd(III)-Oxalate Coordination Framework by Fang-Wen Lv, Mei-Xin Hong, Xue-Ting Wang, Haiquan Tian, Chun-Chang Wang, Xiu-Ying Zheng

“…We have synthesized a series of Ln(III)-based metal-organic framework (MOF) <b>Ln-3D</b> (Ln = Gd/Dy) by the slow release of oxalates in situ from organic ligands (disodium edetate dehydrate (EDTA-2Na) and thiodiglycolic acid). …”
Get full text

Assessment of Hydrologic Data Estimates From ERA5 Reanalyses in Benin, West Africa by René Bodjrènou, Luc Ollivier Sintondji, Yekambessoun M' Po N'Tcha, Diane Germain, Francis Esse Azonwade, Fernand Sohindji, Gilbert Hounnou, Edid Amouzouvi, Arthur Freud Segnon Kpognin, Françoise Comandan

“…ABSTRACT In West Africa, the validation of distributed models is limited by the quality and availability of point station data measured in situ. ERA5 is a climate reanalysis product produced by the European Centre for Medium‐range Weather Forecasts (ECMWF) and is suggested to overcome this constraint. …”
Get full text

Interactive effects of multiple stressors in coastal ecosystems by Shubham Krishna, Shubham Krishna, Carsten Lemmen, Serra Örey, Serra Örey, Serra Örey, Jennifer Rehren, Julien Di Pane, Moritz Mathis, Miriam Püts, Sascha Hokamp, Himansu Kesari Pradhan, Himansu Kesari Pradhan, Matthias Hasenbein, Jürgen Scheffran, Kai W. Wirtz

“…This can be achieved by combining in-situ observations from controlled environments (e.g. mesocosm experiments) with modelling approaches.…”
Get full text

Prospective cohort study investigating frequency and risk factors for acute pain 1 day after refractive surgery by Anat Galor, Jodi Lapidus, Winston Chamberlain, Elyana Vittoria Tessa Locatelli, Jason Betz, Brooke M Harkness, Maricarmen Perez-Blanco, Steven J Everist, Siting Chen, Richard Stutzman, Afshan Nanji, Sue A Aicher

“…Acute ocular pain 1 day after surgery was defined as an NRS score of worst pain since surgery ≥3 and this group was compared with individuals with NRS scores&lt;3.Results 251 individuals underwent refractive surgery (89% laser-assisted in situ keratomileusis, n=222; 11% PRK, n=29). Mean age was 35±8 years (range 19 to 60); 60% (n=150) self-identified as female, 80% (n=203) as White, and 36% (n=89) as Hispanic. …”
Get full text