Titanium nanotubes modulate immunophenotyping and cytokine secretion of T cells via IL-17A: a bioinformatic analysis and experimental validation by Jingju Yin, Jingju Yin, Jingju Yin, Jingju Yin, Yunyang Liao, Yunyang Liao, Yunyang Liao, Yunyang Liao, Shaofeng Liu, Shaofeng Liu, Shaofeng Liu, Shaofeng Liu, Bangwei Che, Hanghang Zhu, Hanghang Zhu, Bingbing Yang, Bingbing Yang, Bingbing Yang, Bingbing Yang, Bin Shi, Bin Shi

“…Magnetic bead sorting of CD3+ T cells and transcriptome sequencing were performed to explore the signaling pathways and key immune factors that may influence the related immune responses.ResultsBioinformatics analysis showed that healthy peri-implant tissues were enriched by the most of T-cell subtypes. …”
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Parasitic infection prevalence in tuberculosis patients and their household contacts in the Littoral Region of Cameroon by Lucy Cho Nchang, Chefor Magha, Patience Agwa Fonong, Narcisse Victor Tchamatchoua Gandjui, Nancielle Mbiatong Tchatat, Desmond Akumtoh Nkimbeng, Frank Noel Nietcho, Juluis Visnel Foyet, Fanny Fri Fombad, Tatiana Djikeussi Katcho, Jerome Fru Cho, Achim Hoerauf, Manuel Ritter, Samuel Wanji

“…Conclusion: We observed that 31 % of the TB patients and household contacts are infected with parasites including P. falciparum, Loa loa and Since helminths can downregulate immune responses against bacterial infections and thus affect treatment efficacy, we recommend that diagnosis of parasitic infections should be included during TB diagnosis and treatment programmes, especially in rural areas.…”
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GmWRKY33a is a hub gene responsive to brassinosteroid signaling that suppresses nodulation in soybean (Glycine max) by Mingliang Yang, Mingliang Yang, Chengjun Lei, Chengjun Lei, Chao Ma, Chao Ma, Xiuming Hou, Xiuming Hou, Mingming Yao, Mingming Yao, Liang Mi, Liang Mi, Enliang Liu, Linli Xu, Shukun Wang, Shukun Wang, Chunyan Liu, Chunyan Liu, Qingshan Chen, Qingshan Chen, Dawei Xin, Dawei Xin, Chang Xu, Chang Xu, Jinhui Wang, Jinhui Wang

“…The qRT-PCR analysis confirmed that BR signaling upregulates GmWRKY33a, leading to nodulation suppression and activation of soybean immune responses. In summary, our research revealed that BR suppresses root nodule formation by modulating the immune signaling pathway in soybean roots. …”
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PD-L1/TLR7 dual-targeting nanobody-drug conjugate mediates potent tumor regression via elevating tumor immunogenicity in a host-expressed PD-L1 bias-dependent way by Wei Huang, Ji Zhou, Jia Li, Yakun Wan, Chao Hu, Meng Liu, Pan Xu, Feng Tang, Jianhua Sun, Xiaolu Yu, Yiru Long, Binfan Chen, Yongliang Tong, Mengwen Shan, Xiaomin Jia, Henglei Lu, Runqiu Chen, Jin Ren, Guangyi Jin, Likun Gong

“…The TLR7 agonists induced broad innate immune responses and intratumoral PD-L1 expression on antigen-presenting cells (APCs), and its antitumor effect was dependent on intratumoral delivery. …”
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Optimizing selenium-enriched yeast supplementation in laying hens: Enhancing egg quality, selenium concentration in eggs, antioxidant defense, and liver health by Longfei Zhang, Jianmin Zhou, Uchechukwu Edna Obianwuna, Cheng Long, Kai Qiu, Haijun Zhang, Xiaolong Qi, Shugeng Wu

“…This study evaluated the effects of selenium-enriched yeast (SY) supplementation at various levels on health and production parameters in laying hens, including egg production, egg quality, selenium (Se) concentrations in eggs, liver health, serum biochemical markers, antioxidant function, and immune responses. A total of 360 Hy-Line Brown hens (28 weeks old) were randomly assigned to four dietary groups with six replicates of 15 birds each, monitored over a 12-week feeding trial after a two-week acclimatization period. …”
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Sexual dimorphism in lung transcriptomic adaptations in fetal alcohol spectrum disorders by Vishal D. Naik, Dylan J. Millikin, Daniel Moussa, Hong Jiang, Alexander L. Carabulea, Joseph D. Janeski, Jiahui Ding, Kang Chen, Marta Rodriguez-Garcia, Sunil Jaiman, Stephen A. Krawetz, Gil Mor, Jayanth Ramadoss

“…These data provide novel information in a growing area focused on alcohol effects on the offspring lung and its influence on appropriate fetal/neonatal immune responses and highlights the importance of examining sexual dimorphism in developmental adaptations.…”
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Targeting of the G9a, DNMT1 and UHRF1 epigenetic complex as an effective strategy against pancreatic ductal adenocarcinoma by Daniel Oyon, Amaya Lopez-Pascual, Borja Castello-Uribe, Iker Uriarte, Giulia Orsi, Sofia Llorente, Jasmin Elurbide, Elena Adan-Villaescusa, Emiliana Valbuena-Goiricelaya, Ainara Irigaray-Miramon, Maria Ujue Latasa, Luz A. Martinez-Perez, Luca Reggiani Bonetti, Felipe Prosper, Mariano Ponz-Sarvise, Silvestre Vicent, Antonio Pineda-Lucena, David Ruiz-Clavijo, Bruno Sangro, Urko Aguirre Larracoechea, Tian V. Tian, Andrea Casadei-Gardini, Irene Amat, Maria Arechederra, Carmen Berasain, Jesus M. Urman, Matias A. Avila, Maite G. Fernandez-Barrena

“…CM272, by targeting this epigenetic complex, shows promising therapeutic potential by inducing apoptosis, reprogramming metabolic pathways, and enhancing immune responses. The combination of CM272 with immunotherapy offers a novel, effective treatment strategy for PDAC.…”
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Longitudinal mitochondrial bioenergetic signatures of blood monocytes and lymphocytes improve during treatment of drug-susceptible pulmonary tuberculosis patients by Bridgette M. Cumming, Kelvin W. Addicott, Fernanda Maruri, Fernanda Maruri, Vanessa Pillay, Rukaya Asmal, Sashen Moodley, Beatriz Barreto-Durate, Beatriz Barreto-Durate, Mariana Araújo-Pereira, Mariana Araújo-Pereira, Matilda Mazibuko, Zoey Mhlane, Nikiwe Mbatha, Khadija Khan, Senamile Makhari, Farina Karim, Lauren Peetluk, Alexander S. Pym, Mahomed Yunus S. Moosa, Yuri F. van der Heijden, Yuri F. van der Heijden, Yuri F. van der Heijden, Timothy S. Sterling, Timothy S. Sterling, Bruno B. Andrade, Bruno B. Andrade, Alasdair Leslie, Alasdair Leslie, Alasdair Leslie, Adrie J. C. Steyn, Adrie J. C. Steyn, Adrie J. C. Steyn

“…Network analysis of the bioenergetic parameters of circulating immune cells with serum cytokine levels indicated a highly coordinated immune response at month 6. These findings underscore the importance of metabolic health in combating TB, supporting the need for further investigation of the bioenergetic immunometabolism associated with TB infection for novel therapeutic approaches aimed at bolstering cellular energetics to enhance immune responses and expedite recovery in TB patients.…”
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Multiorgan proteomic analysis of infected animal models predict potential host factors for chikungunya virus by Dongdong Lin, Cong Tang, Junbin Wang, Yun Yang, Hao Yang, Yanan Zhou, Wenhai Yu, Bai Li, Qing Huang, Haixuan Wang, Ran An, Xiaoming Liang, Yuhuan Yan, Longhai Yuan, Xuena Du, Yuxia Yuan, Yanwen Li, Shuaiyao Lu

“…Our findings reveal significant species‐specific similarities and differences in immune responses and metabolic regulation, with proteins like Interferon‐Stimulated Gene 15 (ISG15) and Retinoic Acid‐Inducible Gene I (RIG‐I) playing crucial roles in the anti‐CHIKV defense. …”
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Pan-tumor analysis to investigate the obesity paradox in immune checkpoint blockade by Evan J Lipson, Ludmila Danilova, Elizabeth M Jaffee, Won Jin Ho, Mark Yarchoan, Yasser Ged, Jean Hoffman-Censits, Jennifer Durham, Sanjay Bansal, Chester Kao, Rajat Mohindra, Stephanie L Alden, Tanguy Y Seiwert, Soren Charmsaz, Howard L Li, Kabeer Munjal, Hua-Ling Tsai, Alexei Hernandez, Erin M Coyne, Rachel Garonce-Hediger, Laura Tang, Marina Baretti, Kathryn Howe, G Scott Chandler, Madelena Brancati, Aanika Warner, Ervin Griffin, Mari Nakazawa, Chris Thoburn, Jennifer Gizzi, Nicole E Gross, Elsa Hallab, Sarah S Shin, Aditi Guha

“…To interrogate mechanisms of divergent immune responses in obese and non-obese patients, we examined the relationship among obesity status, clinical responses, and immune profiles from a diverse, pan-tumor cohort of patients treated with ICI-based therapy.Methods From June 2021 to March 2023, we prospectively collected serial peripheral blood samples from patients with advanced or metastatic solid tumors who received ICI as standard of care at Johns Hopkins. …”
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Immune monitoring of trabectedin therapy in refractory soft tissue sarcoma patients - the IMMUNYON study by Paulo Rodrigues-Santos, Paulo Rodrigues-Santos, Paulo Rodrigues-Santos, Paulo Rodrigues-Santos, Paulo Rodrigues-Santos, Paulo Rodrigues-Santos, Jani Sofia Almeida, Jani Sofia Almeida, Jani Sofia Almeida, Jani Sofia Almeida, Jani Sofia Almeida, Jani Sofia Almeida, Luana Madalena Sousa, Luana Madalena Sousa, Luana Madalena Sousa, Patrícia Couceiro, Patrícia Couceiro, Patrícia Couceiro, Patrícia Couceiro, António Martinho, Joana Rodrigues, Ruben Fonseca, Manuel Santos-Rosa, Manuel Santos-Rosa, Manuel Santos-Rosa, Manuel Santos-Rosa, Manuel Santos-Rosa, Paulo Freitas-Tavares, José Manuel Casanova, José Manuel Casanova, José Manuel Casanova, José Manuel Casanova, José Manuel Casanova

“…This study evaluates Trabectedin therapy in 22 refractory STS patients, analyzing progression-free survival (PFS) and immune responses. Immune monitoring included deep immunophenotyping (200+ parameters), gene expression profiling (103 genes), and soluble proteome analysis (99 analytes). …”
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Phase I study of intratumoral administration of CV8102 in patients with advanced melanoma, squamous cell carcinoma of the skin, squamous cell carcinoma of the head and neck, or ade... by Lucie Heinzerling, Michael Fluck, Caroline Robert, Celeste Lebbe, Erika Richtig, Sven D Koch, Ulrike Gnad-Vogt, Tobias Seibel, Thomas Eigentler, Juan Martin-Liberal, Sebastian Ochsenreither, Peter Mohr, Patrick Terheyden, Carsten Weishaupt, Michael Erdmann, Lukas Koch, Ainara Soria, Igor Samoylenko, Jürgen Krauß, Peter Brossart, Franz Georg Bauernfeind, Marina Gonzalez, Peter Wengenmayer, Ioannis Thomas, Artem Poltoratskiy, Marina Sekacheva, Beate Schmitt-Bormann, Gianluca Quintini, Martin Falk, Paula Codó, Arjun Oberoi, Jana Hess, Yulia Semiletova, Casilda Llacer Perez, Oliver Schönborn-Kellenberger

“…Background CV8102, a toll-like receptor 7/8 and RIG I agonist, has demonstrated antitumor immune responses in preclinical studies. We investigated intratumoral (IT) administration of CV8102 in patients with anti-programmed cell death protein-1 (PD-1) therapy-naïve or anti-PD-1 therapy-refractory cutaneous melanoma (cMEL) and in patients with advanced cutaneous squamous cell carcinoma, head and neck squamous cell carcinoma and adenoid cystic carcinoma.Methods This open-label, cohort-based, phase I dose escalation study aimed to establish the maximum tolerated dose (MTD), recommended dose (RD), safety and preliminary efficacy of CV8102 as monotherapy or in combination with a PD-1 inhibitor. …”
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Extracellular acyl-CoA-binding protein as an independent biomarker of COVID-19 disease severity by Stephane Isnard, Stephane Isnard, Tsoarello Mabanga, Tsoarello Mabanga, Léna Royston, Léna Royston, Léna Royston, Carolina A. Berini, Carolina A. Berini, Simeng Bu, Simeng Bu, Orthy Aiyana, Orthy Aiyana, Hansen Feng, Hansen Feng, Bertrand Lebouché, Bertrand Lebouché, Bertrand Lebouché, Bertrand Lebouché, Cecilia T. Costiniuk, Cecilia T. Costiniuk, Joseph Cox, Joseph Cox, Guido Kroemer, Guido Kroemer, Guido Kroemer, Madeleine Durand, Jean-Pierre Routy, Jean-Pierre Routy, Jean-Pierre Routy, the Biobanque Québécoise de la COVID-19 (BQC-19)

“…Autophagy, a cytosolic structure degradation pathway is involved in the maintenance of cellular homeostasis, degradation of intracellular pathogens and generation of energy for immune responses. Acyl-CoA binding protein (ACBP) is a key regulator of autophagy in the context of diabetes, obesity and anorexia. …”
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Cancer cells avoid ferroptosis induced by immune cells via fatty acid binding proteins by Maria Angelica Freitas-Cortez, Fatemeh Masrorpour, Hong Jiang, Iqbal Mahmud, Yue Lu, Ailing Huang, Lisa K. Duong, Qi Wang, Tiffany A. Voss, Claudia S. Kettlun Leyton, Bo Wei, Wai-Kin Chan, Kevin Lin, Jie Zhang, Efrosini Tsouko, Shonik Ganjoo, Hampartsoum B. Barsoumian, Thomas S. Riad, Yun Hu, Carola Leuschner, Nahum Puebla-Osorio, Jing Wang, Jian Hu, Michael A. Davies, Vinay K. Puduvalli, Cyrielle Billon, Thomas P. Burris, Philip L. Lorenzi, Boyi Gan, James W. Welsh

“…Abstract Background Cancer creates an immunosuppressive environment that hampers immune responses, allowing tumors to grow and resist therapy. …”
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Heterologous cAd3-Ebola and MVA-EbolaZ vaccines are safe and immunogenic in US and Uganda phase 1/1b trials by Myra Happe, Amelia R. Hofstetter, Jing Wang, Galina V. Yamshchikov, LaSonji A. Holman, Laura Novik, Larisa Strom, Francis Kiweewa, Salim Wakabi, Monica Millard, Colleen F. Kelley, Sarah Kabbani, Srilatha Edupuganti, Allison Beck, Florence Kaltovich, Tamar Murray, Susanna Tsukerman, Derick Carr, Carl Ashman, Daphne A. Stanley, Aurélie Ploquin, Robert T. Bailer, Richard Schwartz, Fatim Cham, Allan Tindikahwa, Zonghui Hu, Ingelise J. Gordon, Nadine Rouphael, Katherine V. Houser, Emily E. Coates, Barney S. Graham, Richard A. Koup, John R. Mascola, Nancy J. Sullivan, Merlin L. Robb, Julie A. Ake, Kirsten E. Lyke, Mark J. Mulligan, Julie E. Ledgerwood, Hannah Kibuuka, the VRC 208 and RV 422 study team

“…Different prime-boost intervals impacted the magnitude of humoral and cellular immune responses. The results from these studies demonstrate promising implications for use of these vaccines in both prophylactic and outbreak settings.…”
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An allelic atlas of immunoglobulin heavy chain variable regions reveals antibody binding epitope preference resilient to SARS-CoV-2 mutation escape by Weiqi Deng, Weiqi Deng, Xuefeng Niu, Ping He, Ping He, Qihong Yan, Qihong Yan, Huan Liang, Yongping Wang, Yongping Wang, Lishan Ning, Lishan Ning, Zihan Lin, Zihan Lin, Yudi Zhang, Xinwei Zhao, Xinwei Zhao, Liqiang Feng, Linbing Qu, Ling Chen, Ling Chen

“…BackgroundAlthough immunoglobulin (Ig) alleles play a pivotal role in the antibody response to pathogens, research to understand their role in the humoral immune response is still limited.MethodsWe retrieved the germline sequences for the IGHV from the IMGT database to illustrate the amino acid polymorphism present within germline sequences of IGHV genes. …”
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Effective cancer immunotherapy combining mRNA-encoded bispecific antibodies that induce polyclonal T cell engagement and PD-L1-dependent 4-1BB costimulation by Oana Hangiu, Oana Hangiu, Oana Hangiu, Rocío Navarro, Susana Frago, Laura Rubio-Pérez, Laura Rubio-Pérez, Laura Rubio-Pérez, Antonio Tapia-Galisteo, Antonio Tapia-Galisteo, Antonio Tapia-Galisteo, Laura Díez-Alonso, Laura Díez-Alonso, Laura Díez-Alonso, Marina Gómez-Rosel, Marina Gómez-Rosel, Marina Gómez-Rosel, Noelia Silva-Pilipich, Noelia Silva-Pilipich, Lucía Vanrell, Cristian Smerdou, Cristian Smerdou, Kenneth A. Howard, Laura Sanz, Luis Álvarez-Vallina, Luis Álvarez-Vallina, Luis Álvarez-Vallina, Marta Compte

“…Bispecific T cell-engaging (TCE) antibodies are an effective way to provide tumor-specific T cell receptor-mediated signaling to tumor-infiltrating lymphocytes. mRNA-based delivery of bispecific antibodies, offer a novel approach to enhance tumor-specific immune responses while minimizing adverse effects.MethodsTwo bispecific antibodies were generated: the EGFR x CD3 TCE antibody (LiTE) and the PD-L1 x 4-1BB costimulatory antibody (LiTCo), which was further fused to a high FcRn albumin variant (Albu-LiTCo). …”
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Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1β by Mengmeng Xu, Mengmeng Huang, Guodong WANG, Jingzhe HAN, Xinxin NIU, Jinze HAN, Hangbo YU, Yulong ZHANG, Runhang LIU, Ziwen WU, Hongyu Cui, Yanping Zhang, Suyan Wang, Yongzhen Liu, Yuntong Chen, Yulu Duan, Liuan Li, Yulong Gao, Xiaole Qi

“…Pro-IL-1β is an important inflammatory factor and is also a biomarker for detecting early pro-inflammatory immune responses. However, commercially available antibodies against chicken inflammatory factors are lacking, which prohibits an in-depth exploration of the mechanism of chicken inflammation. …”
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Comparative analysis of the performance, egg quality and ovarian immune function of fast and slow feather strains in tianfu green shell laying hens at various stages of egg product... by Zhi Hu, Hengyong Xu, Zhipeng Zhang, Yuxiang Lu, Yuxin Zhou, Jiajun Zhu, Qingqing Deng, Xi Wang, Yiping Liu, Yao Zhang, Yan Wang

“…These findings indicate that although LF hens excel in early growth stages, EF hens exhibit superior egg production capabilities and enhanced immune responses.…”
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Mucosal SARS-CoV-2 S1 adenovirus-based vaccine elicits robust systemic and mucosal immunity and protects against disease in animals by Najwa D. Aljehani, Levi Tamming, Muhammad Yasir Khan, Rwaa H. Abdulal, Mohamed A. Alfaleh, Aishah Ghazwani, Asalah Helal, Reem M. Alsulaiman, Mohammad A. Sanki, Khalid Alluhaybi, Farah Ayman Sukareh, Rahaf H. Alharbi, Faris H. Alyami, M-Zaki ElAssouli, Salima Shebbo, Wesam H. Abdulaal, Abdullah Algaissi, Ahmad Bakur Mahmoud, Mohammad Basabrain, Diana Duque, Jegarubee Bavananthasivam, Wangxue Chen, Lisheng Wang, Simon Sauve, Turki S. Abujamel, Tarfa Altorki, Rowa Alhabbab, Anh Tran, Xuguang Li, Anwar M. Hashem

“…Our findings indicate that both vaccinations were effective in stimulating strong and long-lasting immune responses, both locally and across the body, when administered through either IM or IN methods. …”
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