Semen quality and fertility effectiveness of cloned buffalo (Bubalus bubalis) bulls by Manoj Kumar Singh, Smiriti Gupta, Kanika Gandhi, S.S. Lathwal, Naresh L. Selokar

“…In conclusion, with this limited study, we can say that the semen produced by cloned bulls is similar to that of non-cloned bulls and parent bulls, which can be further used in various assisted reproductive technologies and sustainable milk production. …”
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Self-reported determinants for subjective financial distress: a qualitative interview study with German cancer patients by Eva Winkler, Katja Mehlis, Bastian Surmann, Wolfgang Greiner, Andrea Züger, Viktoria Mathies, Sophie Pauge, Luise Richter, Thomas Ernst, Natalja Menold

“…The interviews were audiorecorded, transcribed verbatim and analysed using qualitative content analysis.Setting Participants were recruited from two German academic cancer centres, that is, the National Center for Tumor Diseases Heidelberg and Jena University Hospital.Participants 18 patients who had undergone cancer-related therapy for at least 2 months were interviewed (10 females).Results Based on the results of the qualitative content analysis, we developed a multicomponent construct of determinants that could influence subjective financial distress. …”
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Characterization of microplastics in human follicular fluid and assessment of their potential impact on mouse oocyte maturation in vitro by Danyu Ni, Kaidi Yu, Nan Yan, Xiaojiao Chen, Qijun Xie, Yuqin Yang, Wei Jiang, Ye Yang, Junqiang Zhang, Xiufeng Ling

“…Based on the diameter and concentration ranges of MPs in hFF, we used seven types of fluorescence-labeled MP beads—PE, PVC, PP, PS, CPE, Polymethylmethacrylate (PMMA), and Polytetrafluoroethylene (PTFE)—to assess their impact on mouse oocyte in vitro maturation. …”
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Phase 1 study of safety, tolerability, and efficacy of intradermal DNA vaccine ASP2390 in adults allergic to house dust mites by Thomas Kayser, MD, BS, Ronald Smulders, MD, PhD, Tomohiro Kusawake, PhD, Erik Wambre, PhD, Gurunadh R. Chichili, PhD, Mary B. Blauwet, DrPH, Anna Spence, MS, Melanie Patton, BS, Rima Tabash, PhD, Hannah A. DeBerg, PhD, Sugandhika Khosa, MS, Philipp Badorrek, MD, Jens M. Hohlfeld, MD, Brian C. Ferslew, PharmD, PhD

“…The novel LAMP (lysosomal-associated membrane protein)-based DNA vaccine ASP2390 targets major HDM allergens, potentially shifting immune responses toward nonallergic pathways and minimizing the risk of atopy, with positive safety and efficacy signals in preclinical models. Objective: We evaluated the safety, tolerability, and efficacy of first-in-human intradermal ASP2390 in adults with HDM allergy. …”
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Comparative Effects of Glenzocimab and Eptifibatide on Bleeding Severity in 2 Mouse Models of Intracranial Hemorrhage by Sébastien Dupont, Héloïse Lebas, Sabrina Mavouna, Eloïse Pascal, Astride Perrot, Adrien Cogo, Marie‐Charlotte Bourrienne, Carine Farkh, Mialitiana Solo Nomenjanahary, Véronique Ollivier, Fatima Zemali, Bernhard Nieswandt, Stéphane Loyau, Martine Jandrot‐Perrus, Eric Camerer, Jean‐Philippe Desilles, Mikael Mazighi, Yacine Boulaftali, Benoît Ho‐Tin‐Noé

“…Yet while the efficacy of such adjuvant strategies has been shown to be highly time dependent, antiplatelet therapy at the acute phase of ischemic stroke cannot be envisioned until the diagnosis of stroke and its ischemic nature have been confirmed because of the presumed risk of worsening bleeding in case of intracranial hemorrhage (ICH). Here, we investigated this risk for 2 antiplatelet drugs currently being tested in clinical trials for ischemic stroke, glenzocimab and eptifibatide, in 2 mouse models of ICH. …”
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Circulating tumor DNA for surveillance in high-risk melanoma patients: a study protocol by Magnús P.B. Obinah, Sarah A. Al-Halafi, Karin Dreisig, Tim S. Poulsen, Christoffer Johansen, Thomas Litman, Stig E. Bojesen, Estrid Høgdall, Annette H. Chakera, Lisbet R. Hölmich

“…Results and Interpretation: For validation purposes, we conducted a small pilot study using blood samples from 10 patients who had experienced recurrence and had a clinically confirmed BRAF V600E mutation. …”
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Peptide-based vaccine targeting IL17A attenuates experimental spondyloarthritis in HLA-B27 transgenic rats by Tetsuya Tomita, Hiromi Rakugi, Hiroki Hayashi, Ryuichi Morishita, Jiao Sun, Yuka Yanagida, Shota Yoshida, Satoshi Baba, Akiko Tenma, Masayoshi Toyoura, Sotaro Kawabata, Takako Ehara, Ryoko Asaki, Makoto Sakaguchi, Hideki Tomioka, Munehisa Shimamura, Hironori Nakagami

“…Enzyme-linked immunospot (ELISpot) assays for IL4- and interferon-γ-secreting splenocytes were conducted to evaluate IL17A-specific T cell activation. We also evaluated the effect of IL17A vaccine in SpA therapeutic model.Results The IL17A peptide-based vaccine with alum adjuvant successfully induced antibody production and suppressed the arthritis score and joint thickness. …”
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Gastric ESD under Heparin Replacement at High-Risk Patients of Thromboembolism Is Technically Feasible but Has a High Risk of Delayed Bleeding: Osaka University ESD Study Group by Toshiyuki Yoshio, Tsutomu Nishida, Naoki Kawai, Kiyonori Yuguchi, Takuya Yamada, Takamasa Yabuta, Masato Komori, Shinjiro Yamaguchi, Shinji Kitamura, Hideki Iijima, Shusaku Tsutsui, Tomoki Michida, Eiji Mita, Masahiko Tsujii, Tetsuo Takehara

“…This is a retrospective study comprising approximately 1310 consecutive gastric neoplasms in 1250 patients, who underwent ESD in 5 institutes. We assessed the clinical findings and outcomes of ESD under HR, compared to ESD without HR as control. …”
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Clinical Features of Ischemic Enteritis Diagnosed by Double-Balloon Endoscopy by Masanao Nakamura, Takeshi Yamamura, Keiko Maeda, Tsunaki Sawada, Yasuyuki Mizutani, Eri Ishikawa, Naomi Kakushima, Kazuhiro Furukawa, Takuya Ishikawa, Eizaburo Ohno, Takashi Honda, Hiroki Kawashima, Masatoshi Ishigami, Mitsuhiro Fujishiro

“…This study aimed to describe the clinical characteristics, endoscopic imaging features, and treatments for IE at our center. Patients and Methods. We retrospectively searched the DBE database (1,521 patients) at Nagoya University Hospital for patients with IE and collected data regarding endoscopic findings, clinical background, and histological findings. …”
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The effectiveness and risks of Treating people with Idiopathic Pulmonary fibrosis with the Addition of Lansoprazole (TIPAL): study protocol for a randomised placebo-controlled mult... by Toby M Maher, A John Simpson, Anthony Cahn, David Thickett, Luke Vale, Allan B Clark, Nazia Chaudhuri, Ian Forrest, Lisa G Spencer, Christopher Ward, Stephen Jones, Ganesh Raghu, Andrew M Wilson, Helen Parfrey, Megan Jones, Matthew Hammond, Jaclyn Ann Smith, Shajahan Wahed

“…Although several observational studies and a pilot study have investigated the role of proton pump inhibitors (PPIs) in IPF, their efficacy is unknown and there is much debate in international IPF guidelines on their use. We aim to undertake an adequately powered double-blind placebo-controlled randomised multicentre clinical trial to assess the change in forced vital capacity (FVC), cough and other important patient-reported outcomes, following 12-month therapy with PPIs in people with IPF.Methods and analysis A total of 298 patients with IPF diagnosed by a multidisciplinary team according to international guidelines who are not receiving PPIs will be enrolled. …”
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Polo-like Kinase 1 Expression as a Biomarker in Colorectal Cancer: A Retrospective Two-Center Study by Lana Jajac Brucic, Vesna Bisof, Majana Soce, Marko Skelin, Ivan Krecak, Andjela Nadinic, Branka Vrbicic, Zivana Puljiz, Suzana Hancic, Slavko Gasparov

“…<b>Methods</b>: Patients with sporadic CRC, aged >18 years, were included in this study. We categorized the patients into two groups: patients younger than 50 years, and those aged 50 years or older. …”
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Multi-Center Outcome Analysis of 16 Face Transplantations – A Retrospective OPTN Study by Leonard Knoedler, Thomas Schaschinger, Tobias Niederegger, Gabriel Hundeshagen, Gabriel Hundeshagen, Adriana C. Panayi, Curtis L. Cetrulo, Maxime Jeljeli, Elena Hofmann, Max Heiland, Steffen Koerdt, Alexandre G. Lellouch, Alexandre G. Lellouch, Alexandre G. Lellouch

“…Facial Vascularized Composite Allotransplantation (fVCA) restores form and function for patients with severe facial disfigurements, yet multi-center outcome data remain scarce. We accessed the Organ Procurement and Transplantation Network (OPTN) database from 2008 to 2024 to identify all full- or partial-face fVCA recipients, excluding patients under 18 years and those with physiologically impossible BMIs. …”
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Case report: JAK inhibitor treatment of immune dysregulation symptoms in a patient with PTPN2 deficiency by Anna Roppelt, Ulyana Markina, Irina Beloglazova, Irina Beloglazova, Vasily Parshin, Dmitry Kanner, Dmitry Pershin, Mariia Fadeeva, Elena Raykina, Maxim Aleksenko, Alexander Karaulov, Mariana Lysenko, Mariana Lysenko, Daria Fomina, Daria Fomina, Daria Fomina

“…However, the mechanism of pathogenesis for these complications suggests this possibility. We report on a 32-year-old male patient with interstitial lung disease, cytopenia, and lymphadenopathy accompanied by de-novo deletion in PTPN2. …”
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Population genetic analysis of clinical Mycobacterium abscessus complex strains in China by Xiangchen Li, Xiangchen Li, Yelei Zhu, Yewei Lu, Kunyang Wu, Yang Che, Xiaomeng Wang, Weixin Wang, Junli Gao, Junshun Gao, Zhengwei Liu, Zhengwei Liu, Zhuxian Zhou

“…BackgroundTo explore the genetic characteristics of the Mycobacterium abscessus complex (MABC) population in China, given its rising clinical importance among nontuberculous mycobacteria.MethodsWe conducted population genetic analyses on 360 MABC genomes from China, focusing on core genome multilocus sequence typing (cgMLST), pan-genome characterization, population genetics, and antimicrobial resistance gene profiling.ResultsOur analysis identified 273 M. abscessus subsp. abscessus (MabA) and 87 M. abscessus subsp. massiliense (MabM) isolates, uncovering 68 sequence types (STs), with ST5 being the most common. cgMLST classified 33.3% of isolates into six dominant circulating clones (DCCs) and 49.4% into 59 genomic clusters at a threshold of 25 different alleles, including 18 international clusters linking Chinese isolates with seven other countries. …”
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Granulocyte-Macrophage Colony-Stimulating Factor-Activated Neutrophils Express B7-H4 That Correlates with Gastric Cancer Progression and Poor Patient Survival by Zhi-guo Shan, Zong-bao Yan, Liu-sheng Peng, Ping Cheng, Yong-sheng Teng, Fang-yuan Mao, Kun Fan, Yuan Zhuang, Yong-liang Zhao

“…Here, immunohistochemistry, real-time PCR, and flow cytometry analyses were performed to examine levels and phenotype of neutrophils in samples from 41 patients with GC, and also isolated, stimulated, and/or cultured neutrophils for in vitro regulation assays. Finally, we performed Kaplan-Meier plots for overall survival by using the log-rank test to evaluate the clinical relevance of neutrophils and their subsets. …”
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Identification and functional analysis of Wall-Associated Kinase genes in Nicotiana tabacum by Ling Li, Linggai Cao, Jintao Li, Zhiqiang Zhang, Zhiqiang Zhang, Jie Liu, Zhongying Ren, Jie Zhang, Rengang Wang, Yangfan Miao, Shizhou Yu, Wei Li, Wei Li

“…Kinase activity of the WAK proteins was evaluated through in vitro assays. ResultsWe conducted a comprehensive genome-wide identification and analysis of the WAK gene family in tobacco (Nicotiana tabacum). …”
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Genetic ancestry concordant RNA splicing in prostate cancer involves oncogenic genes and associates with recurrence by Muthana Al Abo, Wen-Chi Foo, Lauren E. Howard, Shannon McGue, Bonnie Lacroix, Julie Kephart, Angela Clayton, Blair Thornburg, Monika Anand, Michael B. Rothberg, Shannon J. McCall, Jiaoti Huang, Thomas A. Esther, Judd W. Moul, Michael N. Ferrandino, Thomas J. Polascik, Cary N. Robertson, Brant A. Inman, Andrew J. Armstrong, Yuan Wu, Terry Hyslop, Daniel J. George, Steven R. Patierno, Jennifer A. Freedman

“…Comparing the molecular landscape of PCa among Black and White patients has the potential to identify targets for development of new precision medicine interventions. Herein, we conducted transcriptomic analysis of prostate tumors and paired tumor-adjacent normals from self-reported Black and White PCa patients and estimated patient genetic ancestry. …”
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Clinical findings of acute necrotizing esophagitis complicated by diabetic ketoacidosis by Yuichiro Iwamoto, Tomohiko Kimura, Takashi Itoh, Shigehito Mori, Taku Sasaki, Mana Ohnishi, Haruka Takenouchi, Hideyuki Iwamoto, Junpei Sanada, Yoshiro Fushimi, Yukino Katakura, Fuminori Tatsumi, Masashi Shimoda, Shuhei Nakanishi, Tomoatsu Mune, Kohei Kaku, Hideaki Kaneto

“…The remaining 23 constituted the non-ANE group. We compared the clinical parameters and computed tomography (CT) between the groups. …”
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Carboxylated Graphene: An Innovative Approach to Enhanced IgA-SARS-CoV-2 Electrochemical Biosensing by Luciana de Souza Freire, Ariamna María Dip Gandarilla, Yonny Romaguera Barcelay, Camila Macena Ruzo, Barbara Batista Salgado, Ana P. M. Tavares, Francisco Xavier Nobre, Julio Nino de Souza Neto, Spartaco Astolfi-Filho, Ștefan Țălu, Pritesh Lalwani, Niranjan Patra, Walter Ricardo Brito

“…The characterization of the CG was performed using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Raman spectroscopy, thermogravimetric analysis (TGA), and X-ray diffraction (XRD). We modified screen-printed carbon electrodes (SPCEs) with CG to immobilize the SARS-CoV-2 N-protein, facilitating targeted detection of IgA antibodies (IgA-SARS-CoV-2). …”
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Malnutrisi Energi Protein Berat: Laporan Kasus by Peter Prayogo Hsieh, Putri Dariyanti Wijaya, Putri Aziana, Prahara Wahyu Purnomo, Sitti Radhiah

“…This case is written to give an overview about severe malnutrition in children so that we, as healthcare workers will be more aware to children’s nutrition. …”
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