Multi-tissue network analysis reveals the effect of JNK inhibition on dietary sucrose-induced metabolic dysfunction in rats by Hong Yang, Cheng Zhang, Woonghee Kim, Mengnan Shi, Metin Kiliclioglu, Cemil Bayram, Ismail Bolar, Özlem Özdemir Tozlu, Cem Baba, Nursena Yuksel, Serkan Yildirim, Shazia Iqbal, Jihad Sebhaoui, Ahmet Hacımuftuoglu, Matthias Uhlen, Jan Boren, Hasan Turkez, Adil Mardinoglu

“…However, the intricate mechanisms underlying these interventions remain incompletely understood given their multifaceted effects across multiple tissues. In this study, we challenged rats with sucrose-sweetened water and investigated the potential effects of JNK inhibition by employing network analysis based on the transcriptome profiling obtained from hepatic and extrahepatic tissues, including visceral white adipose tissue, skeletal muscle, and brain. …”
Get full text

Identification of microbial exopolymer producers in sandy and muddy intertidal sediments by compound-specific isotope analysis by Hubas, Cédric, Gaubert-Boussarie, Julie, D’Hondt, An-Sofie, Jesus, Bruno, Lamy, Dominique, Meleder, Vona, Prins, Antoine, Rosa, Philippe, Stock, Willem, Sabbe, Koen

“…Our study reveals that EPS sources are more diverse in sandy sediments than in muddy sediments. We also found distinct patterns in the production and breakdown of EPS in sandy and muddy environments. …”
Get full text

Comparative impact of tertiary lymphoid structures and tumor-infiltrating lymphocytes in cholangiocarcinoma by Chun-Nan Yeh, Yu-Chan Chang, Yi-Chen Yeh, Yu-Chao Wang, Dennis Shin-Shian Hsu, Meng-Lun Lu, Michael Hsiao, Ming-Huang Chen, Nai-Jung Chiang, Shin-Yi Chung, Chien-Jung Huang, Ming-Hsien Chan, Tzu-Sheng Hsu, Yi-Ping Hung

“…This study elucidates their contributions to the TME.Methods We examined 16 tumor samples from a single-arm, phase II trial of nivolumab plus modified gemcitabine and S-1 and various datasets. …”
Get full text

Distinct Neutralising and Complement-Fixing Antibody Responses Can Be Induced to the Same Antigen in Haemodialysis Patients After Immunisation with Different Vaccine Platforms by Nadezhda Wall, Rachel Lamerton, Fiona Ashford, Marisol Perez-Toledo, Aleksandra Jasiulewicz, Gemma D. Banham, Maddy L. Newby, Sian E. Faustini, Alex G. Richter, Haresh Selvaskandan, Roseanne E. Billany, Sherna F. Adenwalla, Ian R. Henderson, Max Crispin, Matthew Graham-Brown, Lorraine Harper, Adam F. Cunningham

“…The SARS-CoV-2 pandemic provided an opportunity to examine the magnitude and functionality of antibody responses in HD patients to a previously unencountered antigen—Spike (S)-glycoprotein—after vaccination with different vaccine platforms (viral vector (VV); mRNA (mRV)). <b>Methods:</b> We compared the total and functional anti-S antibody responses (cross-variant neutralisation and complement binding) in 187 HD patients and 43 healthy controls 21–28 days after serial immunisation. …”
Get full text

Venous thromboembolism incidence and risk factors associated with immune checkpoint inhibitors among patients with advanced non-small cell lung cancer by Chi Nguyen, Alok A Khorana, John Barron, Jennell Palaia, Lisa Rosenblatt, Radhika Pisupati, Ning Huang, Kerrin Gallagher, T Christopher Bond

“…Immune checkpoint inhibitors (ICIs) are a new standard of care for the treatment of lung cancer, but their association with VTE is not fully understood. We evaluated the incidence of VTE and risk factors for patients with advanced non-small cell lung cancer (aNSCLC) treated with first-line ICI-based, chemo-based, or ICI+chemo regimens.Methods This retrospective cohort study used HealthCore Integrated Research Environment - Oncology data, an integrated database of administrative claims, coupled with clinical data from a cancer-care quality program. …”
Get full text

Gastrointestinal symptoms and bowel habits in 53 046 healthy Danish blood donors: a nationwide cross-sectional study by Christian Erikstrup, Ole Birger Pedersen, Henrik Hjalgrim, Erik Sørensen, Jens Frederik Dahlerup, Klaus Rostgaard, Mikkel Steen Petersen, Sisse Rye Ostrowski, Susan Mikkelsen, Khoa Manh Dinh, Thorsten Brodersen, Janna Nissen, Anne Karmisholt Grosen, Jens Kjærgaard Boldsen, Simon Mark Dahl Baunwall, Mie Topholm Bruun, Bitten Aagaard, Christina Mikkelsen, Christian Lodberg Hvas

“…Objective To characterise gastrointestinal symptoms and bowel habits in healthy blood donors and explore symptom phenotypes and their associated factors.Methods Between November 2020 and March 2023, 53 046 participants in the nationwide Danish Blood Donor Study completed a questionnaire including 13 gastrointestinal symptoms, defaecation pattern regularity, stool frequency, and stool consistency. We used a data-driven approach to explore symptom phenotypes and investigated associated factors by multinomial logistic regression.Results Among the 53 046 participants (52% women), 68% (95% CI 67.5% to 68.3%) reported at least one of 13 gastrointestinal symptoms. …”
Get full text

Corticostriatal glutamate‐mediated dynamic therapeutic efficacy of electroacupuncture in a parkinsonian rat model by Xinxin Jiang, Min Sun, Yitong Yan, Yanhua Wang, Xinyu Fan, Jing Wei, Ke Wang, Peirong Liang, Zirui Wang, Jihan Wang, Xiaomin Wang, Jun Jia

“…To establish optimal treatment parameters of EA, motor behaviours were dynamically assessed using open field and rotarod tests. Additionally, we evaluated corticostriatal spine plasticity using immunoelectron microscopy and measured the levels of dopaminergic and glutamatergic neurotransmitters through microdialysis, in vivo electrochemistry and high‐performance liquid chromatography. …”
Get full text

Study protocol on risk factors for the diagnosis of gestational diabetes mellitus in different trimesters and their relation to maternal and neonatal outcomes (GDM-RIDMAN) by Khalida Ismail, Boon How Chew, Iliatha Papachristou Nadal, Angus Forbes, Nurul Iftida Basri, Rozita Zakaria, Pamela Phui Har Yap, Veronika Rysinova, Intan Nureslyna Samsudin, Nurain Mohd Noor, Ziti Akthar Supian, Haslinda Hassan, Fuziah Paimin, Siti Rohani Mohamed Alias, Norizzati Bukhary Ismail Bukhary, Madeleine Benton

“…However, the association of risk factors with GDM diagnosis, maternal and neonatal health outcomes is less established when compared with women without GDM. We aim to examine the diagnostic accuracy of the conventional and novel risk factors for a GDM diagnosis and their impact on maternal and neonatal health outcomes.Methods and analysis This retrospective cohort and nested case–control study at six public health clinics is based on medical records and questionnaire survey of women between 2 and 12 months postpartum. …”
Get full text

Reinforcing cancer immunotherapy with engineered porous hollow mycobacterium tuberculosis loaded with tumor neoantigens by Jie Jiang, Feng-Ying Huang, Shu-Zhen Dai, Guang-Hong Tan, Ming-Hui Chen, Hengyu Chen, Wu-Ping Zheng, Ri-Hong Wu, Weijing Xie

“…Mycobacterium tuberculosis (MTb) contains pathogen-associated molecular patterns (PAMPs) recognized by APCs and adhesion molecules that facilitate MTb invasion of APCs. Therefore, we suggest using MTb as a carrier to enhance APC phagocytosis of neoantigens, thereby promoting antigen cross-presentation.Methods The successful preparation of the MTb carrier (phMTb) was confirmed through electron and confocal microscopy. …”
Get full text

Pentavalent Antimonials Combined with Other Therapeutic Alternatives for the Treatment of Cutaneous and Mucocutaneous Leishmaniasis: A Systematic Review by Taisa Rocha Navasconi Berbert, Tatiane França Perles de Mello, Priscila Wolf Nassif, Camila Alves Mota, Aline Verzignassi Silveira, Giovana Chiqueto Duarte, Izabel Galhardo Demarchi, Sandra Mara Alessi Aristides, Maria Valdrinez Campana Lonardoni, Jorge Juarez Vieira Teixeira, Thaís Gomes Verziganassi Silveira

“…In this systematic review we investigated publications that described a combination of nonconventional treatment for cutaneous and mucocutaneous with pentavalent antimonials. …”
Get full text

Adipose-Derived Stromal Cells for Treatment of Patients with Chronic Ischemic Heart Disease (MyStromalCell Trial): A Randomized Placebo-Controlled Study by Abbas Ali Qayyum, Anders Bruun Mathiasen, Naja Dam Mygind, Jørgen Tobias Kühl, Erik Jørgensen, Steffen Helqvist, Jens Jørgen Elberg, Klaus Fuglsang Kofoed, Niels Groove Vejlstrup, Anne Fischer-Nielsen, Mandana Haack-Sørensen, Annette Ekblond, Jens Kastrup

“…We aimed to evaluate the effect of intramyocardial injections of autologous VEGF-A165-stimulated adipose-derived stromal cells (ASCs) in patients with refractory angina. …”
Get full text

Human proximal tubular epithelial cell interleukin-1 receptor signalling triggers G2/M arrest and cellular senescence during hypoxic kidney injury by Kurt T. K. Giuliani, Purba Nag, Benjamin C. Adams, Xiangju Wang, Seokchan Hong, Anca Grivei, Rebecca L. Johnston, Nicola Waddell, Kenneth K. C. Ho, Yilin Tian, Muhammad Ali Khan, Chang Seong Kim, Monica S. Y. Ng, Glenda Gobe, Jacobus P. J. Ungerer, Josephine M. Forbes, Helen G. Healy, Andrew J. Kassianos

“…Abstract Hypoxia and interleukin (IL)-1β are independent mediators of tubulointerstitial fibrosis, the histological hallmark of chronic kidney disease (CKD). Here, we examine how hypoxia and IL-1β act in synergy to augment maladaptive proximal tubular epithelial cell (PTEC) repair in human CKD. …”
Get full text

Physicians&rsquo; Perceptions of the Quality of Palliative Care and of the Importance of Collaboration in Interdisciplinary Teams in Norwegian Nursing Homes by Skomakerstuen Ødbehr L, Hov R, Sanaker H, Sandsdalen T

“…Further work is needed to ensure that systemic factors enable physicians to deliver high-quality palliative care and that a comfortable physical environment is created in nursing homes.Plain language summary: In this study, we interviewed twelve nursing home physicians and asked them about their views on the quality of palliative care in nursing homes and what they thought about the importance of working in interdisciplinary teams to provide the best possible care for patients. …”
Get full text

Phenotypic and Genotypic Characterization of Extended Spectrum Beta-Lactamase-Producing Clinical Isolates of Escherichia coli and Klebsiella pneumoniae in Two Kenyan Facilities: A... by Sylvia M. Maveke, Gabriel O. Aboge, Laetitia W. Kanja, Alfred O. Mainga, Naftaly Gachau, Beatrice W. Muchira, Gervason A. Moriasi

“…The aim of this study was to assess the phenotypic and genetic determinants of antimicrobial resistance of ESBL-producing Escherichia coli and Klebsiella pneumoniae isolates from patient samples in two Kenyan Hospitals. Methods. We collected 138 E. coli and 127 K. pneumoniae isolates from various clinical specimens at the two health facilities from January 2020 to February 2021. …”
Get full text

Chronic Mild Hyperglycemia in GCK-MODY Patients Does Not Increase Carotid Intima-Media Thickness by Stepanka Pruhova, Petra Dusatkova, Pavel J. Kraml, Michal Kulich, Zdena Prochazkova, Jan Broz, Jaroslav Zikmund, Ondrej Cinek, Michal Andel, Oluf Pedersen, Torben Hansen, Jan Lebl

“…The risk of macrovascular complications is considered low, but studies are limited. We, therefore, investigated the carotid intima-media thickness (CIMT) as an indicator of macrovascular complications in a group of patients with GCK-MODY. …”
Get full text

Knowledge, Attitude, and Barriers Influencing Seasonal Influenza Vaccination Uptake by Mazin A. Barry, Khalid I. Aljammaz, Abdulaziz A. Alrashed

“…Those who elicited symptomatic reaction to the vaccine (26.0%) were less inclined to take it again P=0.035. Conclusion. We concluded that there is a low influenza vaccine uptake rate among our study population, considering that the barriers most commonly chosen by participants are solvable with health education and campaigns oriented towards delivering facts about the vaccine and dispelling misinformation; such measures are highly recommended and are postulated to carry a great benefit that should target common misconceptions identified in this study.…”
Get full text

Increase in methemoglobin fraction due to the use of glyceryl trinitrate patches in preterm infants: a case report and literature review by Andrea Calandrino, Andrea Calandrino, Francesco Vinci, Francesco Vinci, Marcella Battaglini, Marcella Battaglini, Samuele Caruggi, Samuele Caruggi, Chiara Andreato, Chiara Andreato, Carolina Montobbio, Marianna Vannati, Diego Minghetti, Giorgia Brigati, Luca Antonio Ramenghi, Luca Antonio Ramenghi

“…MeHb levels normalized after 18 h, and after adjusting therapy, ischemia resolved successfully.DiscussionWe report the case of a premature IUGR infant who developed elevated methaemoglobin (MeHb) levels during topical GTN therapy for catheter-related extremity ischemia. …”
Get full text

Neospora caninum infection specifically suppresses the expression of a host lncRNA XR_001919077.1 to facilitate parasite propagation by modulating host cell mitochondrial function... by Shan-Shan Zhao, De-Liang Tao, Jin-Ming Chen, Ming-Yi Zhang, Xin Yang, Jun-Ke Song, Qun Liu, Guang-Hui Zhao

“…Our previous study found that N. caninum infection induced significantly aberrant expression of lncRNA profiles in caprine endometrial epithelial cells (EECs). In the present study, we found that N. caninum infection specifically suppressed the expression of a novel lncRNA, XR_001919077.1, and knockdown of XR_001919077.1 with small interfering RNA significantly promoted the propagation of N. caninum in caprine EECs. …”
Get full text

First detection and molecular characterization of Jingmen tick virus with a high occurrence in Rhipicephalus (Boophilus) microplus collected from livestock in Cameroon (2024) by Paloma Kiwan, Eva Lopez, Morena Gasparine, Geraldine Piorkowski, Agathe Colmant, Achille Paguem, Stephanie Mvodo, Laurence Thirion, Xavier de Lamballerie, Remi Charrel, Alessandra Falchi

“…Multiple sequence analysis revealed that JMTV from the Akonolinga area shared > 95% identity with strains from Guinea, and that these strains clustered phylogenetically together. Conclusions We provide molecular evidence of the presence of JMTV in R. …”
Get full text

NPT100-18A rescues mitochondrial oxidative stress and neuronal degeneration in human iPSC-based Parkinson’s model by Julian E. Alecu, Veronika Sigutova, Razvan-Marius Brazdis, Sandra Lörentz, Marios Evangelos Bogiongko, Anara Nursaitova, Martin Regensburger, Laurent Roybon, Kerstin M. Galler, Wolfgang Wrasidlo, Beate Winner, Iryna Prots

“…Conclusions Our findings demonstrate that NPT100-18A limits neuronal degeneration in a human in vitro model of PD. In addition, we provide first mechanistic insights into how a compartment-specific antioxidant effect in mitochondria might contribute to the neuroprotective effects of NPT100-18A.…”
Get full text