Extracellular Recordings of Patterned Human Pluripotent Stem Cell-Derived Cardiomyocytes on Aligned Fibers by Junjun Li, Itsunari Minami, Leqian Yu, Kiyotaka Tsuji, Minako Nakajima, Jing Qiao, Masato Suzuki, Ken Shimono, Norio Nakatsuji, Hitetoshi Kotera, Li Liu, Yong Chen

“…To create tissue-like constructs of CMs for extracellular monitoring, we placed aligned fibers (AFs) on the surface of a microelectrode array and then seeded hiPSC-CMs for subsequent monitoring for 14 days. …”
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Identification and Characterization of a Cryptic Genomic Deletion-Insertion in EYA1 Associated with Branchio-Otic Syndrome by Hao Zheng, Jun Xu, Yu Wang, Yun Lin, Qingqiang Hu, Xing Li, Jiusheng Chu, Changling Sun, Yongchuan Chai, Xiuhong Pang

“…In a sporadic deaf patient diagnosed as BOS, we identified an apparent heterozygous genomic deletion spanning the first four coding exons and one 5′ noncoding exon of EYA1 by targeted next-generation sequencing of 406 known deafness genes. …”
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Observed upper-ocean structure and seasonal production in the southern Kerguelen Plateau region, 1994-2021 by Man Liang, Annie Foppert, Annie Foppert, Karen J. Westwood, Karen J. Westwood, Sophie Bestley, Sophie Bestley

“…Enhanced biological productivity associated with the southern plateau supports an important marine ecosystem with many foraging marine predators. We collate ship-based hydrographic data on the vertical structure of the upper water column near 80°E from eight voyages spanning 1994 to 2021, from 58°S towards the Antarctic continent. …”
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Shift in the profile and prognostic implications in invasive fungal rhinosinusitis: experience of 10 years in a tertiary center by Raúl Mellidez-Acosta, Lidia Torres-García, Miguel Saro-Buendía, Natalia Jaramillo-Ángel, Javier Cabrera-Guijo, Catalina Bancalari-Díaz, Noelia Muñoz-Fernández, Lucas Fito-Martorell, Miguel Armengot-Carceller, Alfonso García-Piñero

“…Evidence on how to optimize its management is still limited. We present a case series from our institution aiming to fill the knowledge gap. …”
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SSVEP Enhancement in Mixed Reality Environment for Brain–Computer Interfaces by Jieyu Wu, Feng He, Xiaolin Xiao, Runyuan Gao, Lin Meng, Xiuyun Liu, Minpeng Xu, Dong Ming

“…Color contrast ratios (CCRs) between stimulus and background colors were introduced to assess the performance difference among all conditions. Additionally, we proposed a cross-correlation task-related component analysis based on simulated annealing (SA-xTRCA), which can increase the signal-to-noise ratio (SNR) and detection accuracy by aligning SSVEP trials. …”
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Anti-Inflammatory Effect of a Peptide Derived from the Synbiotics, Fermented Cudrania tricuspidata with Lactobacillus gasseri, on Inflammatory Bowel Disease by Jimyeong Ha, Hyemin Oh, Nam Su Oh, Yeongeun Seo, Joohyun Kang, Min Hee Park, Kyung Su Kim, Shin Ho Kang, Yohan Yoon

“…These results indicate that Pep 2 derived from αs2-casein was effective in alleviating IBD-associated inflammation. Thus, we showed that these peptides can alleviate inflammation in IBD.…”
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Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue Metabolism by M. Arévalo-Turrubiarte, L. González-Dávalos, A. Yabuta, J. D. Garza, J. L. Dávalos, O. Mora, A. Shimada

“…The main adipogenic transcription factor PPARγ possesses high affinity to 2,4-TZD, a member of the Thiazolidinedione family of insulin-sensitizing compounds used as adipogenic agents. We evaluated 2,4-TZD’s effect on bovine growth and PPAR tissue expression. …”
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Matrix Metalloproteinase-2 Isoforms Differ within the Aortic Wall of Ascending Aortic Aneurysms Associated with Bicuspid Aortic Valve by Ramona Schmitt, Anke Tscheuschler, Philipp Laschinski, Philipp Discher, Jana Fuchs, Fabian A. Kari

“…Influenced by hemodynamics, the tissue levels of proteins associated with aTAA might differ between aTAAs with BAV and TAV and between different localities within the aortic wall. We therefore analyzed aTAA tissue levels of MMP-2 (matrix metalloproteinase-2) isoforms (Pro-MMP-2, active MMP-2, and total MMP-2) and tissue levels of MMP-14, TIMP-2 (tissue inhibitor of metalloproteinase-2), MMP-9, and TIMP-1 in 19 patients with BAV and 23 patients with TAV via gelatin zymography and enzyme-linked immunosorbent assay (ELISA), respectively. …”
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Sunlight exposure practice and its associated factors among infants in Ethiopia, systematic review and meta-analysis. by Fanos Yeshanew Ayele, Natnael Kebede, Asnakew Molla Mekonen, Mengistu Mera Mihiretu, Yawkal Tsega, Elsabeth Addisu, Niguss Cherie, Tesfaye Birhane, Chala Daba, Ahmed Hussien Asfaw, Zinet Abegaz, Abel Endawekie, Anissa Mohammed, Dagnachew Melak, Fekade Demeke Bayou, Husniya Yasin, Aregash Abebayehu Zerga, Fikre Bayu Gebeyehu, Birhanu Wagaye

“…Publication bias was assessed by begg's and Egger's tests. We use Trim and fill analysis was used to treat the publication bias.…”
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Effects of tropical cyclone Freddy on the social determinants of health: the narrative review of the experience in Malawi by Joseph Okeibunor, Otim Patrick Cossy Ramadan, Ishata Nannie Conteh, Abdou Salam Gueye, Jayne Byakika-Tusiime, Annie Chauma-Mwale, Ebenezer Obi Daniel, Dick Chamla, Fiona Braka, Neema Kimambo Rusibamayila, Chol Thabo Yur, Emmanuel Maurice Ochien, Mathew Kagoli

“…Additionally, it aimed to document the immediate humanitarian responses in the acute phase of the disaster. We identified, used and extracted information and data from relevant documents available from the government records, WHO and other multiagency documents, which were summarised along with the humanitarian actions and the associated implications of the entire event. …”
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Preoperative Cardiac Variables of Diastolic Dysfunction and Clinical Outcomes in Lung Transplant Recipients by Ajay Yadlapati, Joseph P. Lynch, Rajan Saggar, David Ross, John A. Belperio, Stephen Weigt, Abbas Ardehali, Tristan Grogan, Eric H. Yang, Jamil Aboulhosn

“…Patients with moderate or severe ventricular systolic dysfunction are typically excluded from lung transplantation; however, there is a paucity of data regarding the prognostic significance of abnormal left ventricular diastolic function and elevated pretransplant pulmonary pressures. Methods. We reviewed the characteristics of 111 patients who underwent bilateral and unilateral lung transplants from 200 to 2009 in order to evaluate the prognostic significance of preoperative markers of diastolic function, including invasively measured pulmonary capillary wedge pressure (PCWP) and echocardiographic variables of diastolic dysfunction including mitral A>E and A′>E′. …”
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Uptake kinetics and distribution of flupyrimin by rice (Oryza sativa L.): Effects of subcellular fractionation and soil factors by Xugen Shi, Min Wang, Zhenyu Jiang, Ruqiang Cui, Baotong Li, Xianpeng Zhang, Lianhu Zhang, Duantao Cao

“…Elucidating the absorption and distribution properties of flupyrimin in rice will help assess the potential risks of human exposure to flupyrimin via the food chain. Here, we studied the uptake kinetics and transport mechanisms of flupyrimin in rice plants grown under hydroponic and soil conditions. …”
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The landscape of alternative polyadenylation during EMT and its regulation by the RNA-binding protein Quaking by Daniel P. Neumann, Katherine A. Pillman, B. Kate Dredge, Andrew G. Bert, Caroline A. Phillips, Rachael Lumb, Yesha Ramani, Cameron P. Bracken, Brett G. Hollier, Luke A. Selth, Traude H. Beilharz, Gregory J. Goodall, Philip A. Gregory

“…Using 3’ end anchored RNA sequencing, we mapped the alternative polyadenylation (APA) landscape following Transforming Growth Factor (TGF)-β-mediated induction of EMT in human mammary epithelial cells and found APA generally causes 3’UTR lengthening during this cell state transition. …”
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Ampelopsin induces MDA-MB-231 cell cycle arrest through cyclin B1-mediated PI3K/AKT/mTOR pathway in vitro and in vivo by Meng Minjun, Yang Qiaolu, Ouyang Zhong, Yang Qingmo, Wu Xinyi, Huang Yufan, Su Yonghui, Chen Shuanglong, Chen Wenlin

“…PI3K/AKT/mTOR plays a very important role in tumor cell growth and proliferation and we hypothesize that AMP may inhibit this pathway. …”
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Osteoarthritic Synovial Fluid Modulates Cell Phenotype and Metabolic Behavior In Vitro by Eduardo Branco de Sousa, Gilson Costa dos Santos Junior, Ramon Pinheiro Aguiar, Rafaela da Costa Sartore, Ana Carolina Leal de Oliveira, Fabio Ceneviva Lacerda Almeida, Vivaldo Moura Neto, Diego Pinheiro Aguiar

“…Cells were cultured for 21 days, characterized with specific markers for MSC, and exposed to a specific cocktail to induce chondrogenic, osteogenic, and adipogenic differentiation. Then, we performed a MTT assay exposing SF cells from non-OA and OA patients to a medium containing non-OA and OA synovial fluid. …”
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Bi-targeting of thioredoxin 1 and telomerase by thiotert promotes cell death of myelodysplastic syndromes and lymphoma by Qiangan Jing, Yunyi Wu, Yanchun Li, Chaoting Zhou, Junyu Zhang, Jun Xia, Keyi Li, Yuhuan Shen, Hongfeng Yao, Xiangmin Tong, Jing Du, Lushan Yu, Ying Wang

“…Abstract Thioredoxin1 (TRX1) and telomerase are both attractive oncology targets that are tightly implicated in tumor initiation and development. Here, we reported that the 6-dithio-2-deoxyguanosine analog thiotert exhibits an effective cytotoxic effect on myelodysplastic syndromes (MDS) cell SKM-1 and lymphoma cell U-937. …”
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Relationship between sleep disorders and depressive symptoms among young women in Japan: a web-based cross-sectional study by Yuta Suzuki, Yukio Urabe, Noriaki Maeda, Satoshi Arima, Tsubasa Tashiro, Rami Mizuta, Takeru Abekura, Sakura Oda, Satoshi Onoue

“…This study aimed to examine the association between sociodemographic factors, lifestyle choices, sleep-related characteristics and depressive symptoms in young Japanese women.Design Web-based cross-sectional study.Participants We distributed an online survey aiming to recruit young Japanese women ages 18 to 29 from 8 November 2022 to 2 February 2023.Primary and secondary outcome measures Sociodemographic, lifestyle, health, and sleep characteristics were compared among participants according to the presence or absence of depressive symptoms, as assessed by the Patient Health Questionnaire 2 (Cut-off value: 3 points).Results Overall, 540 participants aged between 19 and 29 years were enrolled in the study. …”
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Induced mesenchymal stem cells generated from periodontal ligament fibroblast for regenerative therapy by Hemanathan Vembuli, Sheeja Rajasingh, Patrick Nabholz, Jefferson Guenther, Brian R. Morrow, Margaret M. Taylor, Marziyeh Aghazadeh, Vinoth Sigamani, Johnson Rajasingh, Johnson Rajasingh, Johnson Rajasingh

“…However, their limited differentiation capacity and wound healing abilities necessitate the exploration of alternative cell sources. In this study, we generated induced pluripotent stem cells (iPSCs) using a safe, nonviral and mRNA-based approach from human periodontal ligament fibroblasts (PDLF), an easily accessible cell source. …”
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Ex vivo biotransformation of lady’s mantle extracts via the human gut microbiota: the formation of phenolic metabolites and their impact on human normal and colon cancer cell lines... by Katarzyna Jakimiuk, Aleksandra Kruk, Marta Kinga Lemieszek, Jakub W. Strawa, Sebastian Granica, Adrian Wiater, Michał Tomczyk

“…(Rosaceae) stands out as a promising herbal drug candidate due to its phytochemicals displaying anti-inflammatory and antioxidant properties.MethodsIn our study, we investigated the interaction between the human gut microbiota and lady’s mantle herb extract (AV) following the biotransformation of the extract’s constituents and their impact on colorectal cancer cells (HT-29) and normal CCD 841 CoN epithelial cells. …”
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5′-UTR allelic variants and expression of the lycopene-ɛ-cyclase <i>LCYE</i> gene in maize (<i>Zea mays</i> L.) inbred lines of Russian selection by D. Kh. Arkhestova, B. R. Shomakhov, A. V. Shchennikova, E. Z. Kochieva

“…The parallel β,ε-branch is triggered by lycopene-ε-cyclase LCYE, a low expression of which leads to an increase in provitamin A content and is associated with the variability    of the 5’-UTR gene regulatory sequence. In this study, we screened a collection of 165 maize inbred lines of Russian selection for 5’-UTR LCYE allelic variants, as well as searched for the dependence of LCYE expression levels on the 5’-UTR allelic variant in the leaves of 14 collection lines. 165 lines analyzed were divided into three groups carrying alleles A2 (64 lines), A5 (31) and A6 (70), respectively. …”
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