The Mycoplasma hyopneumoniae protein Mhp274 elicits mucosal and systemic immune responses in mice by Mengqi Xie, Zhongshun Huang, Yun Zhang, Yujie Gan, Huiying Li, Dan Li, Honglei Ding

“…Finally, lymphocyte proliferation after stimulation with corresponding proteins or crude extract of M. hyopneumoniae J strain was assessed.ResultsWe successfully constructed recombinant bacteria expressing rMhp170, rMhp274, and rMhp336. …”
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Does preeclampsia impact the gut microbiota of preterm offspring during early infancy? by Yang Chen, Zhou-Ting Yi, Hai-Long Yu, Xiao-Yan Wu, Jun-Ping Wang, Chuan Nie, Hui Li, Sheng-Hui Li, Qiu-Long Yan, Tian-Wen He, Min-Chai Chen, Xin-Yue Yang, Ji-Ying Wen, Li-Juan Lv

“…Fecal samples were collected from preterm neonates of PE and PR mothers at 2 and 6 weeks postpartum and analyzed using shotgun metagenomic sequencing. We found that PE significantly affected the gut microbial composition of preterm neonates, particularly at 2 weeks postpartum. …”
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Predatory bacteria can intensify lung-injury in a multidrug-resistant Acinetobacter baumannii pneumonia model in rat by Zeinab Mohsenipour, Farzaneh Kianian, Behnaz Jahanbin, Hamid Reza Abtahi, Tooba Ghazanfari, Maryam Edalatifard, Saeid Amanpour, Mikael Skurnik, Parya Arazi, Mohammad Mehdi Feizabadi, Mohammad Mehdi Feizabadi

“…Therefore, in this study, we investigated the possibility of using predatory bacteria to improve the Acinetobacter baumannii pneumonia model in rat.MethodsMultidrug-resistant (MDR) A. baumannii clinical strain was used to induce pneumonia. …”
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A tale for two roles: Root-secreted methyl ferulate inhibits P. nicotianae and enriches the rhizosphere Bacillus against black shank disease in tobacco by Siqi Ma, Qianru Chen, Yanfen Zheng, Tingting Ren, Rui He, Lirui Cheng, Ping Zou, Changliang Jing, Chengsheng Zhang, Yiqiang Li

“…Results This study investigated the role of the root exudate methyl ferulate in controlling tobacco black shank disease. We observed that methyl ferulate was secreted in greater quantities in the tobacco resistant cultivar Gexin 3 following inoculation with P. nicotianae than in the susceptible cultivar Xiaohuangjin 1025. …”
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Secondary neoplasms in survivors of pediatric acute lymphoblastic leukemia and lymphoblastic lymphoma: a single-center, retrospective study by Eri Ushida, Hidemi Toyoda, Atsushi Kohso, Yosuke Okumura, Kaori Niwa, Takahiro Ito, Mari Morimoto, Ryo Hanaki, Keishiro Amano, Shotaro Iwamoto, Takao Deguchi, Hiroki Hori, Masahiro Hirayama

“…The aim of this study was to evaluate the incidence of, risk factors for, and outcomes of secondary neoplasms in long-term survivors of ALL and LBL at a tertiary pediatric oncology center in Mie prefecture, Japan.ProcedureWe retrospectively reviewed 188 patients with ALL and LBL who were treated with an ALL-based therapeutic regimen at Mie University Hospital from January 1, 1977 to December 31, 2022 and followed up.ResultsTen patients developed secondary neoplasms, with 10-year and 20-year cumulative incidences of 2.9% [standard error (SE) = 1.5%] and 5.5% (SE = 2.3%), respectively. …”
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Enabling ultra-flexible inorganic thin-film-based thermoelectric devices by introducing nanoscale titanium layers by Ming Tan, Xiao-Lei Shi, Wei-Di Liu, Yong Jiang, Si-Qi Liu, Tianyi Cao, Wenyi Chen, Meng Li, Tong Lin, Yuan Deng, Shaomin Liu, Zhi-Gang Chen

“…Abstract Here, we design exotic interfaces within a flexible thermoelectric device, incorporating a polyimide substrate, Ti contact layer, Cu electrode, Ti barrier layer, and thermoelectric thin film. …”
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Comparative evaluation of various DNA extraction methods and analysis of DNA degradation levels in commercially marketed Chestnut rose juices and beverages by Yongchao Ren, Yunlong Ma, Yanqi Li, Yun Song, WeiWei Zhao, Xuncai Huang, Danmin Yu, Jian Li, Zuogang Xu, Wenjun Zhao

“…This study aimed to optimize and compare four DNA extraction methods for Chestnut rose juices and beverages. Furthermore, we also conducted a comparison and analysis of the extent of DNA degradation in Chestnut rose juice or beverage by utilizing the amplicon size. …”
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Comparative Genomics Analysis of Plasmid pPV989-94 from a Clinical Isolate of Pantoea vagans PV989 by Lei Xu, Min Yin, Tingyuan Zhu, Yabo Liu, Yuanyuan Ying, Junwan Lu, Chaoqing Lin, Jianchao Ying, Teng Xu, Liyan Ni, Qiyu Bao, Shunfei Lu

“…Pantoea vagans, a gram-negative bacterium from the genus Pantoea and family Enterobacteriaceae, is present in various natural environments and considered to be plant endophytes. We isolated the Pantoea vagans PV989 strain from the clinic and sequenced its whole genome. …”
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Integrative analysis of metabolite and transcriptome reveals the biosynthetic pathway and candidate genes for iridoid glycoside biosynthesis in Neopicrorhiza scrophulariiflora (Pen... by Ke Rao, Ke Rao, Siyu Liu, Siyu Liu, Xiaohui Tang, Guofu Jia, Shaohua Yang, Chaoxiang Ren, Chaoxiang Ren, Jin Pei, Jin Pei

“…Through qRT-PCR validation, we found that three NsF3H/NsF3D genes, four NsUGD/NsUPD genes, one Ns2HFD gene, and three NsSQM genes may participate in the iridoid biosynthesis pathway. …”
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The success rate of infrazygomatic mini-implants placed with and without surgical guides: a historical control study by Lixian Yuan, Ruifeng Zhao, Jingjing Liu, Rui Tang, Shizhu Bai, Xin Liu

“…However, there is no a surgical guide which can be applied for the insertion of infrazygomatic mini-implant. In this study, we introduced a two-trajectories surgical guide and investigated the success rate of infrazygomatic mini-implants under the guidance of the template. …”
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Synthetic CT generation from CBCT and MRI using StarGAN in the Pelvic Region by Paritt Wongtrakool, Chanon Puttanawarut, Pimolpun Changkaew, Supakiet Piasanthia, Pareena Earwong, Nauljun Stansook, Suphalak Khachonkham

“…The goal was to provide accurate Hounsfield unit (HU) data for dose calculation to enable MRI simulation and adaptive radiation therapy (ART) using CBCT or MRI. We also compared the performance and benefits of StarGAN to the commonly used CycleGAN. …”
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An automated information extraction model for unstructured discharge letters using large language models and GPT-4 by Robert M. Siepmann, Giulia Baldini, Cynthia S. Schmidt, Daniel Truhn, Gustav Anton Müller-Franzes, Amin Dada, Jens Kleesiek, Felix Nensa, René Hosch

“…However, challenges were encountered, and the prompt was fine-tuned in a second experiment to improve the results. We further tested whether open-source LLMs can achieve similar results. …”
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H3K9 post-translational modifications regulate epiblast/primitive endoderm specification in rabbit blastocysts by Wilhelm Bouchereau, Hong-Thu Pham, Worawalan Samruan, Van-Hong Vu, Thierry Joly, Marielle Afanassieff, Pierre Savatier, Rangsun Parnpai, Nathalie Beaujean

“…However, the role of these modifications in lineage segregation in the mammalian blastocyst remains poorly understood. We demonstrate that di- and tri-methylation marks, H3K9me2 and H3K9me3, decrease during cavitation and expansion of the rabbit blastocyst. …”
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Biofilmed-PGPR: Next-Generation Bioinoculant for Plant Growth Promotion in Rice under Changing Climate by Jeberlin Prabina Bright, Hemant S. Maheshwari, Sugitha Thangappan, Kahkashan Perveen, Najat A. Bukhari, Debasis Mitra, Riyaz Sayyed, Andrea Mastinu

“…Further, Pearson correlation analysis showed that yield was positively correlated with soil dehydrogenase (r = 0.92**) and soil chlorophyll content (r = 0.96**). We concluded that CBB could be used as a novel biofilm-based bio-inoculant to increase rice productivity and crop fitness as a component in integrated nutrient management and sustainable organic farming strategies with reduced chemical fertilizers.…”
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Trends and factors associated with delays in the first prenatal consultation in Guinea by Tiany Sidibe, Mamadou Dioulde Balde, Sadan Camra, Ramata Diallo, Bienvenu Salim Camara, Karifa Kourouma, Madeleine Toure, Kaba Saran Keita, Fanta Barry, Maimouna Balde

“…The factors associated with the delay in performing the ANC1 were: being aged 35–49 years (adjusted odds ratio [AOR]: 1.36; 95% confidence interval [CI]: 1.08–1.69); Living in a poor household (AOR: 1.87; 95% CI: 1.64–2.13), living in Boké (AOR: 2.13; 95% CI: 1.58–2.87), and N’zerekore (AOR: 4.97; 95% CI: 3.58–6.91). Conclusion: We recommend stepping up door-to-door awareness-raising activities by community relays and ensuring that the policy of free antenatal care in Guinea is effective. …”
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Impact on clinical outcomes, surgical interventions, anaesthetic decisions and complication rates following implementation of the NICE obstructive sleep apnoea guidelines during pr... by Gabrielle Shaw, Ricki Leggatt, Paige Roberts, Amanda Peace Witton, Nicole Moll, Akshay Dwarakanath

“…NICE (National Institute for Health and Care Excellence) recommends a rapid preoperative assessment for patients undergoing elective surgery. Methods: We have evaluated the impact on implementing the NICE guidelines on clinical outcomes, surgical interventions, anaesthetic decisions and complication rates in surgical patients referred from the pre-assessment clinic prior to an elective intervention. …”
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Effect of phosphorus fractions on benthic chlorophyll-a: Insight from the machine learning models by Yuting Wang, Sangar Khan, Zongwei Lin, Xinxin Qi, Kamel M. Eltohamy, Collins Oduro, Chao Gao, Paul J. Milham, Naicheng Wu

“…Different machine learning models have been used to predict the benthic chl-a from the TP and dissolved P (DP), however, to the best of our knowledge, colloidal and particulate P (CP and PP) have never been used in predictive models for benthic chl-a. To address this gap, we applied two machine learning algorithms—random forest (RF), and artificial neural networks (ANN) to predict benthic chl-a concentrations by incorporating these specific P fractions as separate variables. …”
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Assessment of Risk Factors for Fractures in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from Northeast China by Yan Guo, Yingfang Wang, Feng Chen, Jiabei Wang, Difei Wang

“…Our study measured the heel bone mineral density (BMD) and used the timed “up and go” (TUG) test and other indicators. In addition, we performed logistic regression analysis to explore the risk factors for fractures in the general population and the diabetic population and to analyze the differences. …”
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HOLSEA-NL: a Holocene water level and sea level indicator dataset for the Netherlands by K. de Wit, K. M. Cohen, R. S. W. van de Wal, R. S. W. van de Wal

“…However, uniform overviews of these data exist only for smaller local subsets, rather than for the entire Netherlands. In this paper, we present a dataset of 712 Holocene water level indicators from the Dutch coastal plain that are relevant for studying RSLR and regional subsidence, compiled in HOLSEA workbook format (<span class="uri">https://doi.org/10.5281/zenodo.11098446</span>, De Wit and Cohen, 2024). …”
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RAS/BRAF Circulating Tumor DNA Mutations as a Predictor of Response to First-Line Chemotherapy in Metastatic Colorectal Cancer Patients by Jiannan Yao, Wanchun Zang, Yang Ge, Nathaniel Weygant, Pan Yu, Lei Li, Guanhua Rao, Zhi Jiang, Rui Yan, Linjia He, Yang Yu, Mulan Jin, Gang Cheng, Guangyu An

“…Since circulating tumor DNA (ctDNA) offers clear advantages as a minimally invasive method for tumor monitoring compared with tumor tissue, we aimed to evaluate genotyping ctDNA using a next-generation sequencing- (NGS-) based panel to identify the prognostic value of mutation status in metastatic colorectal cancer (mCRC) patients with primary tumor resected and with subsequent lines of treatment in this study. …”
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