Genome constitution and differentiation of subgenomes in Siberian and Far Eastern endemic species of the genus Elymus (Poaceae) according to the sequencing of the nuclear gene waxy by A. V. Agafonov, S. V. Asbaganov, E. V. Shabanova (Kobozeva), I. V. Morozov, A. A. Bondar

“…To determine the species’ genomic constitution and to evaluate the level of phylogenetic differentiation, we examined the GBSS1 gene in 18 species of Elymus from Siberia and the Russian Far East, including the fol- lowing 14 endemics: E. charkeviczii, E. jacutensis, E. kamczadalorum, E. komarovii, E. kronokensis, E. lenensis, E. macrourus, E. margaritae, E. subfibrosus, E. sajanensis, E. transbaicalensis, E. peschkovae, E. uralensis, and E. viridiglumis.PCR amplification products of GBSS1 gene fragments (including exons 9–14) were cloned and 6–8 clones per accession were sequenced. …”
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Complex high-risk indicated PCI (CHIP-PCI): is it safe to let fellows-in-training perform it as primary operators? by Tim Kinnaird, Zia Ul-Haq, Holly Morgan, Anirban Choudhury, Majd B Protty, Alexander Chase, David Hildick-Smith, Shantu Bundhoo, Saad Hasan, Diluka Premawardhana, Ahmed Hailan, Mohammed Shugaa Addin, Hussain Hussain

“…Whether the outcomes of these cases differ if the primary operator is a supervised FIT compared with a consultant is unknown.Methods Using multicentre PCI data from three cardiac centres in South Wales, UK (2018–2022), we identified 2295 CHIP-PCI cases with a UK-BCIS CHIP Score of 3 or more. …”
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Unveiling the effect of stress on vacancy diffusion isotropy at high temperature in Ni-Re Systems: Insights from atomic simulations by Shichao Du, Siyuan Lin, Wenyue Zhao, Yi Ru, Yanling Pei, Shusuo Li, Shengkai Gong

“…Rhenium (Re), as a slow-diffusing element, exhibits nonnegligible effects on the vacancy diffusion behavior varied with its atomic concentration and position particularly. In this work, we comprehensively study the vacancy diffusion behavior in Ni-Re alloys at 1173 ∼ 1573 K under stress along [001] and [111], by using the Self-Evolving Atomistic Kinetic Monte Carlo (SEAKMC) method with interatomic potentials. …”
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Evaluation of the Ability of Miltefosine Associated with Topical GM-CSF in Modulating the Immune Response of Patients with Cutaneous Leishmaniasis by Fábio Peixoto, Maurício T. Nascimento, Rúbia Costa, Juliana Silva, Gaby Renard, Luiz Henrique Guimarães, Gerson Penna, Manoel Barral-Netto, Lucas P. Carvalho, Paulo R. L. Machado, Edgar M. Carvalho

“…Miltefosine is more effective than pentavalent antimony (Sbv) in the treatment of CL, and here, we evaluate the ability of Sbv, miltefosine, and GM-CSF administered intravenously, orally, or topically, respectively, to modify the immune response. …”
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Immune Reactions Against Elongation Factor 2 Kinase: Specific Pathogenesis of Gastric Ulcer from Helicobacter pylori Infection by Kiyoshi Ayada, Kenji Yokota, Yoshiro Kawahara, Yumiko Yamamoto, Kazuyuki Hirai, Tomoki Inaba, Masahide Kita, Hiroyuki Okada, Kazuhide Yamamoto, Keiji Oguma

“…Helicobacter pylori (H. pylori) infection is a definite causative factor for gastric ulcers (GUs). In the present study we detected a specific antigen of gastric epithelial cells (HGC-27) using cell ELISA, which was recognized by the sera of GU patients (n=20) but not in patients with chronic gastritis (CG; n=20) or in healthy volunteers (HC; n=10). …”
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Impact of MMP2 rs243865 and MMP3 rs3025058 Polymorphisms on Clinical Findings in Alzheimer’s Disease Patients by Vladimira Durmanova, Juraj Javor, Zuzana Parnicka, Gabriel Minarik, Agata Ocenasova, Barbora Vaseckova, Veronika Reznakova, Maria Kralova, Tomas Hromadka, Ivana Shawkatova

“…Among various MMPs, MMP-2 and MMP-3 belong to candidate molecules related to AD pathology. In our study, we aimed to evaluate the association of MMP2 rs243865 and MMP3 rs3025058 polymorphisms with AD susceptibility and their influence on age at onset and MoCA score in patients from Slovakia. …”
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Nerve Enlargement in Patients with INF2 Variants Causing Peripheral Neuropathy and Focal Segmental Glomerulosclerosis by Quynh Tran Thuy Huong, Linh Tran Nguyen Truc, Hiroko Ueda, Kenji Fukui, Koichiro Higasa, Yoshinori Sato, Shinichi Takeda, Motoshi Hattori, Hiroyasu Tsukaguchi

“…<b>Methods</b>: To characterize the peripheral nerve phenotype of INF2-related CMT, we studied the clinical course, imaging, histology, and germline genetic variants in two unrelated CMT-DIE patients. …”
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Incidence of SARS-CoV-2 reinfection in a paediatric cohort in Kuwait by Mohammad Alghounaim, Saadoun Bin-Hasan, Fatemah Alhaddad, Ali Abdulkareem, Danah Alsharrah, Abdullah Alkandari, Mona Al-Ahmad, Hashem Al Hashemi

“…Objective Subsequent protection from SARS-CoV-2 infection in paediatrics is not well reported in the literature. We aimed to describe the clinical characteristics and dynamics of SARS-CoV-2 PCR repositivity in children.Design This is a population-level retrospective cohort study.Setting Patients were identified through multiple national-level electronic COVID-19 databases that cover all primary, secondary and tertiary centres in Kuwait.Participants The study included children 12 years and younger between 28 February 2020 and 6 March 2021. …”
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The proneural transcription factor Atoh1 promotes odontogenic differentiation in human dental pulp stem cells (DPSCs) by Camila Sabatini, Huey-Jiun Lin, Galib Ovik, Richard Hall, Techung Lee

“…The functional versatility of Atoh1 prompted us to test the possibility that Atoh1 may intersect the dental pulp stem cell (DPSC) gene regulatory network governing odontogenic differentiation. Methods We isolated DPSCs from human dental pulps and treated the cells with a replication-deficient adenoviral vector to achieve robust ectopic expression of Atoh1, following which the growth and odontogenic differentiation profiles of DPSCs were characterized. …”
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Protective factors for suicidal ideation and suicide attempts in adolescence: a longitudinal population-based cohort study examining sex differences by Victoria Bakken, Stian Lydersen, Norbert Skokauskas, Anne Mari Sund, Jannike Kaasbøll

“…Individual, social and environmental protective factors were identified based on prior research and theoretical frameworks. We used linear (or ordinal logistic) regression analyses with SI (or SA) as dependent variable, and one protective factor, sex and their interaction as covariates. …”
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Exacerbation of diabetes due to F. Nucleatum LPS-induced SGLT2 overexpression in the renal proximal tubular epithelial cells by Aiko Seki, Koichiro Kajiwara, Jumpei Teramachi, Masahiko Egusa, Takuya Miyawaki, Yoshihiko Sawa

“…Abstract Background Diabetes treatments by the control of sodium-glucose cotransporter 2 (SGLT2) is commonly conducted while there are still uncertainties about the mechanisms for the SGLT2 overexpression in kidneys with diabetes. Previously, we have reported that glomeruli and proximal tubules with diabetic nephropathy express toll-like receptor TLR2/4, and that the TLR ligand lipopolysaccharide (LPS) of periodontal pathogens have caused nephropathy in diabetic model mice. …”
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Impact of fear of coronavirus disease 2019 on attention-deficit/hyperactivity disorder traits associated with depressive symptoms, functional impairment, and low self-esteem in uni... by Tomoko Suzuki, Toshiyuki Ohtani, Michiko Nakazato, Ariuntuul Garidkhuu, Basilua Andre Muzembo, Shunya Ikeda

“…ADHD traits, fear of COVID-19, depressive symptoms, functional impairment, and self-esteem were assessed using the adult ADHD Self-Report Scale, Fear of COVID-19 Scale, K6 Scale, Sheehan Disability Scale, and Rosenberg Self-Esteem Scale, respectively. We used linear regression analysis and the Paramed command in Stata to analyze whether fear of COVID-19 mediated the association between ADHD traits and outcomes. …”
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Effects of the Implementation of an Emergency Surgical Pattern in Patients with Rhegmatogenous Retinal Detachment: A Retrospective Observational Study by Ziye Chen, Kai Gao, Kunbei Lai, Wenbin Zheng, Jizhu Li, Yaping Liu, Baoyi Liu, Xiaoyue Wei, Yuan Ma, Zitong Chen, Rebiya Tuxun, Tao Li

“…To analyze the effects of the implementation of emergency surgical patterns in patients with rhegmatogenous retinal detachment (RRD) and provide evidence for promoting emergency surgical patterns for RRD. Methods. We reviewed the electronic medical records of 346 patients (348 eyes) who underwent surgical repair of RRD at the Zhongshan Ophthalmic Center in Southern China. …”
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Reasons Why Patients Do Not Return to Sport Post ACLReconstruction: A Cross-Sectional Study by Hamdan M, Haddad BI, Amireh S, Abdel Rahman AMA, Almajali H, Mesmar H, Naum C, Alqawasmi MS, Albandi AM, Alshrouf MA

“…This study aims to investigate the common factors that hinder patients from resuming sports activities following ACLR and to assess how these barriers impact their ability to return to sports. We hypothesized that patients’ psychological factors, including fear of reinjury, would significantly influence their decision to return to sport after ACL reconstruction.Methods: In this cross-sectional study, 138 patients who had undergone ACL reconstruction surgery were examined. …”
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Multi-scale channel attention U-Net: a novel framework for automated gallbladder segmentation in medical imaging by Yiming Zhou, Xiaobo Wen, Xiaobo Wen, Kang Fu, Meina Li, Lin Sun, Xiao Hu

“…ObjectivesTo develop a novel automatic delineation model, the Multi-Scale Channel Attention U-Net (MCAU-Net) model, for gallbladder segmentation on CT images of patients with liver cancer.MethodsWe retrospectively collected the CT images from 120 patients with liver cancer, based on which ground truth was manually delineated by physicians. …”
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Increasing the uptake of long-acting reversible contraception in general practice: the Australian Contraceptive ChOice pRoject (ACCORd) cluster randomised controlled trial protocol by Danielle Mazza, Angela Taft, Kevin McGeechan, Kirsten Black, Heather McKay, Marion Haas, Jayne Lucke, Jeffery F Peipert

“…Using randomisation stratified by whether the GP inserts LARCs or not, a total of 54 groups will be allocated to the intervention (online ‘LARC First’ training and rapid referral pathways) or control arm (usual care). We aim to recruit 729 women from each arm. The primary outcome will be the number of LARCs inserted; secondary outcomes include the women's choice of contraceptive method and quality of life (Short Form Health Survey, SF-36). …”
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Human ACE2 transgenic pigs are susceptible to SARS-CoV-2 and develop COVID-19-like disease by Long Fung Chau, Simon Lillico, Tanja Opriessnig, Rosemary Blake, Luc Tardy, Chen-Hsuin Lee, Scott Maxwell, Claire Warren, Elizabeth Thornton, Catherine L. Mclaughlin, Gerry McLachlan, Christine Tait-Burkard, Sarah Fletcher, Stephen Anderson, Sharon Brown, Louise Gibbard, Thomas Tzelos, Dawn MacMillan-Christensen, J. Kenneth Baillie, David A. Dorward, David J. Griffiths, Finn Grey

“…However, pigs are not susceptible to SARS-CoV-2 infection which limits their utility as a model. To address this, we have developed transgenic pigs expressing human ACE2 that are susceptible to SARS-CoV-2 infection. …”
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A New Device Improves Signs and Symptoms of TMD by Ruggero Cattaneo, Davide Pietropaoli, Barry C. Cooper, Dino Capparè, Eleonora Ortu, Annalisa Monaco

“…Temporomandibular joint dysfunction (TMD) therapy remains an open challenge for modern dentistry. Herein, we propose a new neuromuscular lingual device able to reduce signs and symptoms of TMD in female patients with chronic orofacial pain. …”
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Collaborative design of a care pathway for pharmacy-based PrEP delivery in Nigeria: insights from stakeholder consultation by Obinna I. Ekwunife, Theodora C. Omenoba, Ugochi Eyong, Valentine Okelu, Michael Alagbile, Ifeanyi Ume, Ambrose Eze, Aderinola Fisayo, Gloria Aidoo-Frimpong, Farah Shroff, Chimezie Anyakora

“…Building on the successful pilot implementation of pharmacy-based PrEP delivery in Kenya, we engaged Nigerian stakeholders in adapting the model, addressing user and provider concerns to ensure effective implementation in Nigeria. …”
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Allogeneic DNT cell therapy synergizes with T cells to promote anti-leukemic activities while suppressing GvHD by Jongbok Lee, Hyeonjeong Kang, Branson Chen, Yoosu Na, Ismat Khatri, Fraser Soares, Housheng Hansen He, Arjun D. Law, Tianzhong Pan, Armin Gerbitz, Xiaoyu Zhu, Mark D. Minden, Li Zhang

“…A phase I clinical trial demonstrated the feasibility, safety, and potential efficacy of allogeneic DNT therapy for patients with relapsing acute myeloid leukemia (AML) post-allo-HSCT. Here, we studied the impact of DNTs on the anti-leukemic and GvHD-inducing activities of Tconv cells. …”
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