RfxCas13d-mediated inhibition of Circ1647 alleviates renal fibrosis via PI3K/AKT signaling pathway by Yufang Ni, Hongmin Zhang, Qianwen Xian, Wenjie Qin, Hongwei Su, Li Wang, Jianchun Li

“…This study aimed to investigate the role of Circ1647 in renal fibrosis, which is a hallmark of CKD.Methods In this study, we established a unilateral ureteral obstruction (UUO) model and delivered Circ1647 RfxCas13d knockdown plasmid into renal parenchymal cells via retrograde injection through the ureter followed by electroporation. …”
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[18F]Tetrafluoroborate, a new NIS PET/CT radiopharmaceutical: an overview focused on differentiated thyroid cancer by Arnoldo Piccardo, Francesco Fiz, Sergio Righi, Stefano Raffa, Mattia Riondato, Gianluca Bottoni, Matteo Bauckneht, Michela Massollo, Alessio Rizzo, Mehrdad Shoushtari Zadeh Naseri, Pierpaolo Trimboli, Giorgio Treglia

“…However, these approaches have low sensitivity due to the low resolution of SPECT. 18F-Tetrafluoroborate (TFB) has been proposed as a viable alternative, which could outperform 131/123I scans owing to the superior PET resolution. We have reviewed the literature to collect the available data on TFB diagnostic performance and compare it with the standard methods. …”
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In Silico Screening of Putative Corynebacterium pseudotuberculosis Antigens and Serological Diagnosis for Caseous Lymphadenitis in Sheep by Enzyme-Linked Immunosorbent Assay by Daniela Droppa-Almeida, Caroline de Santana Ferreira, Ioná Brito, Sibele Borsuk, Jorge Alberto López Rodríguez, Isabel Bezerra Lima-Verde, Francine Ferreira Padilha

“…The only proteins expressed were PTS and Ribonuclease. In parallel, we expressed CP40 and all were used with ELISA antigen in 49 CLA positive sera and 26 CLA negative sera. …”
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Prevalence and Economic Impact of Acute Respiratory Failure in the Prehospital Emergency Medical Service of the Madrid Community: Retrospective Cohort Study by Ana María Cintora-Sanz, Cristina Horrillo-García, Víctor Quesada-Cubo, Ana María Pérez-Alonso, Alicia Gutiérrez-Misis

“…By achieving these objectives, we hope to inform efforts to address respiratory failure and establish a standardized methodology and framework that can facilitate expansion to a continuous community-wide registry in Madrid, driving advances in emergency care and care practices in these pathologies. …”
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Effects of Mating and Social Exposure on Cell Proliferation in the Adult Male Prairie Vole (Microtus ochrogaster) by A. E. Castro, L. J. Young, F. J. Camacho, R. G. Paredes, N. F. Diaz, W. Portillo

“…Microtus ochrogaster is a rodent with a monogamous reproductive strategy characterized by strong pair bond formation after 6 h of mating. Here, we determine whether mating-induced pair bonding increases cell proliferation in the subventricular zone (SVZ), rostral migratory stream (RMS), and dentate gyrus (DG) of the hippocampus in male voles. …”
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TESTING POTATO COLLECTION SAMPLES FOR THE PRESENCE OF GENES FOR RESISTANCE TO PHYTOPATHOGENS BY MEANS OF DNA MARKERS by A. B. Saynakova, M. S. Romanova, S. N. Krasnikov, O. V. Litvinchuk, Ya. I. Alekseev, A. V. Nikulin, E. V. Terentjeva

“…The sample included two varieties, three populations produced by self-pollination of the Ideal variety, and 35 individually selected potato hybrids. As a result, we identified marker NL25 (Sen1) in 19 samples; marker PVX (Rx) in 13 samples; marker RYSC3312 (Ryadg) in 10 samples; marker YES3-3A341 (Rysto) in 5 samples; markers TG 689141, 57R450, N195337 (H1) in 12 samples; marker Gro1-4-1602 (Gro1-4) in 6 samples; marker Gpa2-2452 (Gpa2) in 13 samples. …”
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AdipoRon mitigates liver fibrosis by suppressing serine/glycine biosynthesis through ATF4-dependent glutaminolysis by Xiangting Zhang, Yuan Zeng, Huiya Ying, Yiwen Hong, Jun Xu, Rong Lin, Yuhao Chen, Xiao Wu, Weimin Cai, Ziqiang Xia, Qian Zhao, Yixiao Wang, Ruoru Zhou, Dandan Zhu, Fujun Yu

“…Therefore, our objective is to explore the impact of AdipoRon on ATF4-mediated endoplasmic reticulum stress and amino acid metabolism in HSCs. We induced liver fibrosis in mice through intraperitoneal injection of CCl4 and administered AdipoRon (50 mg/kg) via gavage. …”
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RAS, a Pentatricopeptide Repeat Protein, Interacts with OsTRX z to Regulate Chloroplast Gene Transcription and RNA Processing by Zhennan Qiu, Shiyong Wen, Peinan Sun, Dongdong Chen, Chunmiao Wang, Xiliang Song, Liying Xiao, Peiliang Zhang, Dongying Zhao, Cuiping Wen, Peiyan Guan, Xuechu Du, Yinghui Sun, Chenshan Xu, Jian Song

“…Thioredoxin z (TRX z) plays a significant role in chloroplast development by regulating the transcription of chloroplast genes. In this study, we identified a pentatricopeptide repeat (PPR) protein, rice albino seedling-lethal (RAS), that interacts with OsTRX z. …”
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Increased Growth Differentiation Factor 15 Levels Are Associated with HIV-Associated Neurocognitive Impairment: A Pilot Study by Ali Boustani, Mary K. Ford, Jacqueline R. Kulbe, Anna E. Laird, Leeann Shu, Matthew Spencer, Bryant Avalos, Kyle C. Walter, Ronald J. Ellis, Jerel Adam Fields

“…<b>Methods</b>: PWH from the California NeuroAIDS Tissue Network (CNTN) underwent comprehensive neuropsychological exams within 12 months before death and were categorized based on cognitive performance. We examined GDF15 levels in their CSF (Cerebrospinal Fluid) and brain tissues using immunoblotting, immunohistochemistry, double immunolabeling, and ELISA. …”
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Simultaneous vs Staged Procedures for Bilateral Total Knee Arthroplasty: Reduced Infection, Cost, and Readmission Rates Associated With Simultaneous Procedures by Aaron Singh, BA, Travis M. Kotzur, BS, Kathleen Lundquist, MD, Blaire C. Peterson, BS, William H. Young, MD, Chance C. Moore, MD, Frank Buttacavoli, MD

“…This study aims to compare medical and surgical complications and hospital-related outcomes between simultaneous and staged bilateral TKA. We hypothesize that staged procedures will have superior outcomes. …”
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Ultrastructural Analysis of Leishmania infantum chagasi Promastigotes Forms Treated In Vitro with Usnic Acid by João S. B. da Luz, Erwelly B. de Oliveira, Monica C. B. Martins, Nicácio H. da Silva, Luiz C. Alves, Fábio A. B. dos Santos, Luiz L. S. da Silva, Eliete C. Silva, Paloma L. de Medeiros

“…Morphological alterations were observed as blebs in cell membrane and shapes given off, increasing the number of cytoplasmic vacuoles, and cellular and mitochondrial swelling, with loss of cell polarity. We concluded that the usnic acid presented antileishmanial activity against promastigote forms of Leishmania infantum chagasi and structural and ultrastructural analysis reinforces its cytotoxicity. …”
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Effects of management of plastic and straw mulching management on crop yield and soil salinity in saline-alkaline soils of China: A meta-analysis by Ying Song, Jineng Sun, Mingjun Cai, Jinyu Li, Meizhen Bi, Mingxiu Gao

“…Moreover, it remains unclear how these effects vary with climatic, edaphic, and agronomic management factors such as tillage methods, irrigation types, nitrogen application rates, and mulching duration. To address this gap, we conducted a meta-analysis to assess the effects of plastic film mulch (PM), straw mulch (SM), and their combination (SM+PM) on crop yields and soil salinity. …”
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Technological Adjuncts to Streamline Patient Recruitment, Informed Consent, and Data Management Processes in Clinical Research: Observational Study by Jodie Koh, Stacey Caron, Amber N Watters, Mahesh Vaidyanathan, David Melnick, Alyssa Santi, Kenneth Hudson, Catherine Arguelles, Priyanka Mathur, Mozziyar Etemadi

“…MethodsUsing one clinical research study as an example, we highlighted the use of technological adjuncts to automate and streamline research processes across various digital platforms, including a centralized database of electronic medical records (enterprise data warehouse [EDW]); a clinical research data management tool (REDCap [Research Electronic Data Capture]); and a locally managed, Health Insurance Portability and Accountability Act–compliant server. …”
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Work loss in patients with rheumatoid arthritis treated with abatacept, rituximab, tocilizumab or TNF inhibitors: a nationwide direct drug-to-drug comparison by Ulf Lindström, Johan Askling, Katerina Chatzidionysiou, Lotta Ljung, Lars Klareskog, Martin Neovius, Nils Feltelius, Eva Baecklund, Christopher Sjöwall, Helena Forsblad-d’Elia, Carl Turesson, Elisabet Lindqvist, Thomas Frisell, Alf Kastbom, Helena Forsblad-d'Elia, Jonas Söderling, Ann Knight, Gerd-Marie Alenius, Gustaf Magnus Bruze

“…Objective To compare work loss after starting tumour necrosis factor inhibitors (TNFi), rituximab, abatacept or tocilizumab in patients with rheumatoid arthritis (RA).Methods We used data from the Swedish Rheumatology Quality Register to identify patients aged 19-62 years who were treated with TNFi (n=15 093), rituximab (n=2123), abatacept (n=1877) or tocilizumab (n=1720) between 2007 and 2020. …”
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Wybrane czynniki warunkujące wdrażanie polityki spójności Unii Europejskiej w regionie Podkarpacia by Alina Walenia

“…Podstawowym czynnikiem wspierającym sprawną absorpcję środków UE przez beneficjentów z regionu Podkarpacia powinno być wzmocnienie kadrowe służb, w tym przygotowanie merytoryczne pracowników, którzy uczestniczą we wdrażaniu polityki regionalnej i spójności z wykorzystaniem instrumentów finansowych UE. …”
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Myocardial Gene Expression of T-bet, GATA-3, Ror-γt, FoxP3, and Hallmark Cytokines in Chronic Chagas Disease Cardiomyopathy: An Essentially Unopposed TH1-Type Response by Luciana Gabriel Nogueira, Ronaldo Honorato Barros Santos, Alfredo Inácio Fiorelli, Eliane Conti Mairena, Luiz Alberto Benvenuti, Edimar Alcides Bocchi, Noedir Antonio Stolf, Jorge Kalil, Edecio Cunha-Neto

“…Although the T cell-rich myocarditis is known to play a pathogenetic role, the relative contribution of each of the functional T cell subsets has never been thoroughly investigated. We therefore assessed gene expression of cytokines and transcription factors involved in differentiation and effector function of each functional T cell subset (TH1/TH2/TH17/Treg) in CCC, NIC, and heart donor myocardial samples. …”
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Risk prediction models for breast cancer: a systematic review by Jiang Li, He Li, Jie He, Ni Li, Yadi Zheng, Zheng Wu, Maomao Cao

“…Objectives To systematically review and critically appraise published studies of risk prediction models for breast cancer in the general population without breast cancer, and provide evidence for future research in the field.Design Systematic review using the Prediction model study Risk Of Bias Assessment Tool (PROBAST) framework.Data sources PubMed, the Cochrane Library and Embase were searched from inception to 16 December 2021.Eligibility criteria We included studies reporting multivariable models to estimate the individualised risk of developing female breast cancer among different ethnic groups. …”
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IL-6 Inhibition Reduces STAT3 Activation and Enhances the Antitumor Effect of Carboplatin by Zhi-Yong Wang, Jun-Ai Zhang, Xian-Jin Wu, Yan-Fang Liang, Yuan-Bin Lu, Yu-Chi Gao, You-Chao Dai, Shi-Yan Yu, Yan Jia, Xiao-Xia Fu, Xiaoquan Rao, Jun-Fa Xu, Jixin Zhong

“…However, it is not fully understood whether anticytokine therapy could reverse chemosensitivity and enhance the suppressive effect of chemotherapy on tumor growth. In this study, we aimed to investigate the effect of IL-6 inhibition therapy on the antitumor effect of carboplatin. …”
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Fluvial terrace formation in a mountainous area (2): influence of eustatism, tectonics and altitudinal distribution of watersheds based on an allostratigraphic study (Albania) by Mugnier, Jean-Louis, Guzmán, Oswaldo, Vassallo, Riccardo,  Matraku, Kristina, Jouanne, François

“…The thickness of the sedimentary units above the strath surfaces is mostly influenced by the altitudinal distribution of the river catchments: thin strath terraces are found in the low elevation catchments, while thick fill terraces are found in large catchments and we suggest here that the deposition of fill terrace sediments occurred very rapidly at the cold-warm transitions when the high elevation areas of the large catchments were not protected by vegetation and heavily affected by hillslope processes that delivered a large volume of sediments. …”
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Successful Pregnancy Outcome in a Patient Treated with Pembrolizumab and Exposed to Fluoro-Deoxyglucose (¹⁸F-FDG) PET/CT: Case Report and Review of Literature by Anna Lucia Mastricci, Felice Sorrentino, Elisa Giansiracusa, Erika Zanzarelli, Graziana Silvana De Lucia, Vincenza Fernanda Fesce, Luigi Nappi, Lorenzo Vasciaveo

“…The PD-1/PD-L1 pathway, targeted by pembrolizumab, plays a critical role in maternal–fetal immune adaptation, raising concerns about its safety during pregnancy. <b>Case Report:</b> We report the case of a 36-year-old woman diagnosed with rHL who unknowingly became pregnant during treatment with pembrolizumab and ¹⁸F-FDG PET/CT scans. …”
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