Rescue of common and rare exon 2 skipping variants of the GAA gene using modified U1 snRNA by Paolo Peruzzo, Natascha Bergamin, Martina Bon, Sara Cappelli, Alessandra Longo, Elisa Goina, Cristiana Stuani, Emanuele Buratti, Andrea Dardis

“…A functional splicing assay using a GAA hybrid minigene carrying different variants known to affect GAA exon 2 splicing was used to test the effect of engineered U1 snRNAs on exon 2 inclusion. …”
Get full text

Engagement féminin en Kabylie et intersection des revendications (1980-2001). Dominations, expériences et négociations identitaires by Margherita Rasulo

“…From a methodological point of view, with regard to its object and geographical context, this work relies on a hybrid theoretical approach that combines Berber/Amazigh studies (Abrous, 1988, 1995, 2004; Chaker, 1988, 1998; Tilmatine, 1989, 2017; Ould Fella, 2021), Kabyle ethnology studies (Bourdieu, 1998; Lacoste-Dujardin, 1985, 2008) and the experiential approach to the minority condition (Chassain et al., 2016), gender studies and subaltern studies (Spivak, 1988). …”
Get full text

Exosome miR-199a-5p modulated vascular remodeling and inflammatory infiltration of Takayasu’s arteritis by Shuning Guo, Jiehan Li, Shurui Pang, Jing Li, Xinping Tian

“…Dual-luciferase reporter assay, RNA immunoprecipitation assay and fluorescence in situ hybridization were utilized to validate the target gene of miR-199a-5p. …”
Get full text

Modelling the air quality impacts of a zero emission zone scenario in the city of Milan by Andrea Piccoli, Valentina Agresti, Marco Bedogni, Giovanni Lonati, Guido Pirovano

“…The assessment of the environmental and health effects is done thanks to a modelling suite composed of a road traffic, an emission, a hybrid air quality models, plus a fourth module for human health impacts. …”
Get full text

Exploring the Role of C-C Motif Chemokine Ligand-2 Single Nucleotide Polymorphism in Pulmonary Tuberculosis: A Genetic Association Study from North India by Sanjay K. Biswas, Mayank Mittal, Ekata Sinha, Vandana Singh, Nidhi Arela, Bharat Bajaj, Pramod K. Tiwari, Vishwa M. Katoch, Keshar K. Mohanty

“…The genotyping was carried out in 373 participants with pulmonary TB (PTB) and 248 healthy controls (HCs) for CCL2-2518 A>G and -362 G>C polymorphisms by PCR-RFLP and by melting curve analysis using fluorescence-labeled hybridization fluorescent resonance energy transfer (FRET) probes, respectively, followed by DNA sequencing in a few representative samples. …”
Get full text

Implementation strategies: lessons learned during an e-learning intervention to improve dietary behaviors and feeding practices in early childhood education and care by Henrik Lian, Nina Cecilie Øverby, Frøydis Nordgård Vik, Anine Christine Medin, Natalie Garzon Osorio, Christine Helle, Tormod Bjørkkjær, Penelope Love, Harry Rutter, Mary Elizabeth Barker, Elisabet Rudjord Hillesund, Sissel Heidi Helland

“…Methods The study is a part of the Nutrition Now study, a hybrid type 1 non-randomized controlled trial. The implementation process followed the Dynamic Integrated Evaluation Model (DIEM). …”
Get full text

β-Catenin mediated TAM phenotype promotes pancreatic cancer metastasis via the OSM/STAT3/LOXL2 axis by Yijia Zhang, Xinya Zhu, Liyuan Chen, Tianyu Gao, Guang Chen, Jin Zhu, Guoyu Wang, Daiying Zuo

“…These OSM-expressing TAMs exhibited a distinct hybrid M1/M2 phenotype. Besides, our transcriptional profiling of TAMs revealed concurrent activation of both pro- and anti-inflammatory programs, with enrichment in Wnt signaling pathways. …”
Get full text

Neuropilin1-dependent paracrine signaling of cancer cells mediated by miRNA exosomal cargo by Claudia Palazzo, Roberta Mastrantonio, Noemi Gioelli, Erika Testa, Francesco Recco, Donatella Lucchetti, Giulia Villari, Alessio D’Alessio, Alessandro Sgambato, Flavio Mignone, Guido Serini, Maria Teresa Viscomi, Luca Tamagnone

“…The micro-RNA profile carried by exosomes was investigated by Next Generation Sequencing. miRNA-Scope in situ hybridization was used to assess the transfer of miRNA exosome cargo to target cells, and immunofluorescence analysis revealed expression regulation of targeted proteins. miRNA activity was blocked by the use of specific antago-miRs. …”
Get full text

Development of the early fetal human thalamus: from a protomap to emergent thalamic nuclei by Maznah Alhesain, Ayman Alzu’bi, Ayman Alzu’bi, Ayman Alzu’bi, Niveditha Sankar, Niveditha Sankar, Charles Smith, Charles Smith, Janet Kerwin, Ross Laws, Susan Lindsay, Gavin J. Clowry

“…Higher order thalamic nuclei, relaying information between cortical regions and important in higher cognitive function, are greatly expanded.MethodsThis study mapped the emergence of thalamic nuclei in human fetal development (8–16 post conceptional weeks; PCW) by revealing gene expression patterns using in situ hybridization and immunohistochemistry for previously established thalamic development markers.ResultsIn the proliferative thalamic ventricular zone, OLIG3 and NR2F1 immunoreactivity marked the extent of the thalamus, whereas PAX6 and NR2F2 were expressed in gradients, suggesting an early protomap. …”
Get full text

Preliminary effectiveness of social prescription and virtual patient information in increasing tertiary prevention among cancer patients (ESPRIT): protocol for a single-centre, ran... by Karen Steindorf, Michael Koller, Michael Leitzmann, Wolfgang Herr, Daniel Heudobler, Lisa Boyer, Anne Herrmann, Tobias Pukrop, Christine Bernardi, Sabine Einhell

“…Cost-effectiveness and the time spent in the consultation, as well as potential implementation barriers and facilitators, will also be explored as part of a mixed-methods hybrid design. All data will be summarised descriptively. …”
Get full text

Identifikasi Gagal Ginjal Kronis dengan Mengimplementasikan Metode Support Vector Machine beserta K-Nearest Neighbour (SVM-KNN) by Alvin Tarisa Akbar, Novanto Yudistira, Achmad Ridok

“…For this research, we implemented a hybrid of SVM with KNN known as SVM-KNN which was optimized using Simplified Sequential Minimal Optimization (Simplified SMO). …”
Get full text

Comparative Genomics Reveals Evidence of the Genome Reduction and Metabolic Potentials of <i>Aliineobacillus hadale</i> Isolated from Challenger Deep Sediment of the Mariana Trench by Shaofeng Yang, Jie Liu, Yang Liu, Weichao Wu, Jiahua Wang, Yuli Wei

“…The highest average nucleotide identity (ANI), the highest digital DNA-DNA hybridization (DDH) values, and the average amino acid identity (AAI) with <i>Neobacillus</i> sp. …”
Get full text

Analysis of the long-range transport of the volcanic plume from the 2021 Tajogaite/Cumbre Vieja eruption to Europe using TROPOMI and ground-based measurements by P. Hedelt, J. Reichardt, F. Lauermann, B. Weiß, N. Theys, A. Redondas, A. Barreto, O. Garcia, D. Loyola

“…Furthermore, a new modeling approach based on TROPOMI <span class="inline-formula">SO₂</span> VCD measurements and the Hybrid Single-Particle Lagrangian Integrated Trajectory Model (HYSPLIT) was developed, which confirmed the link between Tajogaite eruptions and Lindenberg measurements. …”
Get full text

Selection of terminal Piétrain pigs improves vitality and prevalence of congenital defects in crossbred piglets by Wim Gorssen, Wim Gorssen, Carmen Winters, Roel Meyermans, Léa Chapard, Katrijn Hooyberghs, Jaro De Kort, Alice Van den Broeke, Jürgen Depuydt, Steven Janssens, Nadine Buys

“…The first dataset was collected by the breeding organization and consisted of 12,010 crossbred litters (Piétrain sire, hybrid dam; two farms) scored on piglets’ vitality, number of piglets with porcine congenital splay leg syndrome and scrotal hernia, pre-weaning mortality and the number of piglets born death. …”
Get full text

Deep learning-based synthetic CT for dosimetric monitoring of combined conventional radiotherapy and lattice boost in large lung tumors by Hongwei Zeng, Xiangyu E, Minghe Lv, Su Zeng, Yue Feng, Wenhao Shen, Wenhui Guan, Yang Zhang, Ruping Zhao, Jingping Yu

“…These results indicated that sCT is feasible for inter-fraction dose monitoring and adaptive planning. After rigid and hybrid deformable registration of sCT and pCT, the mean distance-to-agreement was 0.80 ± 0.18 mm, and the mean Dice similarity coefficient was 0.97 ± 0.01. …”
Get full text

Telehomecare Monitoring for Patients Receiving Anticancer Oral Therapy: Protocol for a Mixed Methods Evaluability Study by Dominique Tremblay, Thomas Joly-Mischlich, Annick Dufour, Marie-Claude Battista, Djamal Berbiche, José Côté, Marco Décelles, Catherine Forget, Brigitte Guérin, Manon Larivière, Frédéric Lemay, Manon Lemonde, Éric Maillet, Nathalie Moreau, Michel Pavic, Sara Soldera, Catherine Wilhelmy

“…The objectives are to (1) contextualize how and why TM is implemented according to (a) site characteristics, (b) team characteristics, and (c) characteristics of patients receiving anticancer oral therapy; (2) assess TM effectiveness for recording electronic patient-reported outcome measures (ePROMs) and patient-reported experience measures (ePREMs) according to the site, implementation process, and patient characteristics; (3) describe the acceptability and feasibility of TM from the perspectives of the people directly or indirectly involved and provide evidence-based actionable guidance in anticipation of provincewide implementation. MethodsThis type II hybrid effectiveness-implementation study uses a concurrent mixed methods design. …”
Get full text

Risk Management in Sustainable Construction Projects: A Systematic Review and Bibliometric Analysis by Kasra Hosseini, Youness Javid

“…This focus should be particularly prominent in developing countries and should be approached through experimental and applied studies utilizing mixed methods and hybrid approaches under uncertainty. Various studies have examined barriers and risks in construction projects from different sustainability perspectives, such as economic, environmental, and social. …”
Get full text

Fluid Dynamics in a Thrust Fault Inferred from Petrology and Geochemistry of Calcite Veins: An Example from the Southern Pyrenees by Daniel Muñoz-López, David Cruset, Irene Cantarero, Antonio Benedicto, Cédric M. John, Anna Travé

“…The formation of fractures F1 and F2 and the mosaic to chaotic breccia is consistent with dilatant fracturing within the process zone (around the fault tip) during initial fault growth, whereas the formation of the latest fracture system points to hybrid shear-dilational failure during propagation of the fault. …”
Get full text

Process Evaluations of Interventions for the Prevention of Type 2 Diabetes in Women With Gestational Diabetes Mellitus: Systematic Review by Iklil Iman Mohd Sa'id, Natasha Hotung, Madeleine Benton, Iliatha Papachristou Nadal, Anisah Baharom, Matthew Prina, Barakatun Nisak Mohd Yusof, Kimberley Goldsmith, Samantha Birts, Ching Siew Mooi, Angus Forbes, Khalida Ismail, Boon How Chew

“…The studies involved 3 methods of intervention delivery, including in person (n=7), digital (n=7), and hybrid (n=9). Two of the studies conducted pilot RCTs assessing the feasibility and acceptability of their interventions, including recruitment, participation, retention, program implementation, adherence, and satisfaction, and 1 study assessed the efficacy of a questionnaire to promote food and vegetable intake. …”
Get full text

Impact of incorporating wheat straw together with basal nitrogen ratio on panicle formation and grain yield in rice by Li Qin, Chenyu Wang, Hong Zhao, Shenqi Zhou, Kun Liu, Lijun Liu

“…Straw incorporation and nitrogen (N) application have vital effects on rice yield, but the influence of incorporating wheat straw with N fertilizer ratio on spikelet differentiation, degeneration, and grain yield in rice is not clear. In this experiment, hybrid rice Fengyouxiangzhan (FY) and Yongyou 2640 (YY) were used as materials. …”
Get full text