The Clinicopathological Features and Prognostic Significance of HER2-Low in Early Breast Tumors Patients Prognostic Comparison of HER-Low and HER2-Negative Breast Cancer Stratified... by Sanaa Gamrani, Laila Akhouayri, Sara Boukansa, Mehdi Karkouri, Hinde El Fatemi

“…A new nomenclature has been proposed for HER2 1+ and HER2 2+ tumors that are confirmed negative according to fluorescence in situ hybridization (FISH). These tumors are now referred to as HER2-low, and it is thought that they may represent a distinct subtype of breast cancer that warrants further investigation. …”
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Analysis of the structure and function of the tomato <i>Solanum lycopersicum</i> L. MADS-box gene <i>SlMADS5</i> by A. V. Nezhdanova, M. A. Slugina, E. A. Dyachenko, A. M. Kamionskaya, E. Z. Kochieva, A. V. Shchennikova

“…An in silico expression analysis revealed the absence of gene transcripts in roots, leaves, and shoot apical meristem, and their presence in flowers, fruits, and seeds at different stages of development. Two-hybrid analysis showed the ability of SlMADS5 to activate transcription of the target gene and interact with TAGL1. …”
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The Use of AI in Mental Health Services to Support Decision-Making: Scoping Review by Hassan Auf, Petra Svedberg, Jens Nygren, Monika Nair, Lina E Lundgren

“…The AI systems were used in health care, self-care, and hybrid care contexts, addressing a variety of mental health problems. …”
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Klasifikasi Teks Hadis Bukhari Terjemahan Indonesia Menggunakan Recurrent Convolutional Neural Network (CRNN) by Muhammad Yuslan Abu Bakar, Adiwijaya Adiwijaya

“…This research uses a hybrid method in deep learning by combining a Convolutional Neural Network and a Recurrent Neural Network, namely Convolutional Recurrent Neural Network (CRNN). …”
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The effect of <i>T. aestivum</i> chromosomes 1A and 1D on fertility of alloplasmic recombinant (<i>H. vulgare</i>)-<i>T. aestivum</i> lines depending on cytonuclear compatibility by L. A. Pershina, N. V. Trubacheeva, V. K. Shumny

“…All plants of F1 combinations FS×1R(1A), FS×1R(1D), PS×1R(1A), PS×1R(1D) were sterile, which indicates that a single dose of genes localized on wheat chromosomes 1A or 1D is not enough to restore male fertility in FS and PS plants. All plants of hybrid combinations F(hm)×1R(1A) and F(hm)×1R(1D) in both F1 and F2 were fertile, that is, fertility of allolines with cytonuclear coadaptation does not depend on wheat chromosomes 1A and 1D.…”
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Explaining the Antecedents and Consequences of the Glass Cliff in Service Organizations by Sara Kavoosi, Ali Safari, Ali Shaemi Barzoki

“…Drawing from the qualitative aspect of the study, it is recommended that decision-makers and senior managers in service organizations diligently consider the origins of the glass cliff phenomenon and actively engage in managing the factors that contribute to the unique challenges faced by women in high-risk managerial positions Conclusion This investigation stands as one of the initial studies that, by leveraging a hybrid research methodology, provides a comprehensive framework to thoroughly understand the causal conditions, strategic responses, and consequences of the glass cliff phenomenon.…”
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Comparison of Illumina and Oxford Nanopore sequencing data quality for Clostridioides difficile genome analysis and their application for epidemiological surveillance by Semeh Bejaoui, Sofie Holtsmark Nielsen, Astrid Rasmussen, John Eugenio Coia, Dorte Terp Andersen, Tobias Bruun Pedersen, Martin Vad Møller, Marc Trunjer Kusk Nielsen, Dorte Frees, Søren Persson

“…The goal of our study was to compare sequencing and assembly quality of 37 C. difficile isolates using Illumina (SPAdes assembled) and Nanopore (Flye and Unicycler assembled) data alone, along with hybrid assemblies obtained with short-read polishing of long reads. …”
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Validating a digital depression prevention program for adolescents in Jordan: cultural adaptation and user testing in a randomized controlled trial by Latefa Ali Dardas, Obada Al-leimon, Tracy Gladstone, Abd Arrahman Dabbas, Insaf Alammouri, Benjamin Van Voorhees

“…However, its effectiveness is contingent upon addressing barriers to access, enhancing program interactivity, and integrating hybrid support systems that combine digital tools with in-person guidance. …”
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De Novo Acute Myeloid Leukemia with Combined CBFB-MYH11 and BCR-ABL1 Gene Rearrangements: A Case Report and Review of Literature by Venkata Rakesh Sethapati, Ra’ed Jabr, Leyla Shune, Wissam El Atrouni, Patrick R. Gonzales, Wei Cui, Shivani Golem

“…Fluorescent in situ hybridization studies confirmed these rearrangements. …”
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Models of Integrated Acute Care for Older Adult Inpatients That Incorporate Integrative Health: An Integrative Review by Bonvin E, Perruchoud E, Tacchini-Jacquier N, Perrenoud J, Melly P, Celik S, Jean M, Verloo H

“…Three integrated acute psychogeriatric-care models were identified: the Admiral Nursing model, the Lewy body dementia Admiral nursing service model, and the Care for Acute Mentally Infirm Elders model. A single, hybrid, Integrated, People-Centred Health Services model for acute and community health care was identified. …”
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Humoral and cellular immune durability of different COVID-19 vaccine platforms following homologous/heterologous boosters: one-year post vaccination by Maaweya Awadalla, Halah Z. AlRawi, Rahaf A. Henawi, Fawziya Barnawi, Haitham Alkadi, Ahmed Alyami, Ammar Alsughayir, Alyazeed S. Alsaif, Ayman Mubarak, Wael Alturaiki, Bandar Alosaimi

“…IntroductionThe durability of Hybrid immunity induced by natural infection and/or COVID-19 vaccines and evidence supporting further booster vaccination are crucial factors for pandemic response, yet remain poorly understood.MethodsWe measured the durability of immune response and neutralizing capacity of antibodies following Homologous/Heterologous vaccination by mRNA-based vaccines (Pfizer-BioNTech BNT162b2) or (Moderna mRNA-1273) and viral vector-based vaccines (ChAdox1 nCoV-19-Oxford-AstraZeneca) in infected and non-infected patients. …”
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Multi-omics analysis reveals the interplay between intratumoral bacteria and glioma by Ting Li, Zhanyi Zhao, Meichang Peng, Lu Zhang, Cheng Wang, Feiyang Luo, Meiqin Zeng, Kaijian Sun, Zhencheng Fang, Yunhao Luo, Yugu Xie, Cui Lv, Jiaxuan Wang, Jian-Dong Huang, Hongwei Zhou, Haitao Sun

“…We employed immunohistochemistry, multicolor immunofluorescence, and fluorescence in situ hybridization (FISH) staining to observe the presence and location of bacteria. …”
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Elite Alleles of EPE1 Identified via Genome-wide Association Studies Increase Panicle Elongation Length in Rice by Hao Sun, Qiqi Yao, Mei Hai, Tianhu Li, Jinghan Sun, Zhengbo Liu, Yang Ang, Yingying Zhao, Yanan Zhang, Xianping Cheng, Tao Huang, Yinping Chang, Mingyu Du, Erbao Liu

“…Elite alleles of EPE1 can be used to further improve PEL in CMS lines to increase the outcrossing rate and yield or seed production in hybrid rice breeding.…”
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Quantitative accuracy of preclinical in ovo PET/MRI: influence of attenuation and quantification methods by Theresa Balber, Katarína Benčurová, Manuela Mayrhofer, Joachim Friske, Martin Haas, Claudia Kuntner, Thomas H. Helbich, Marcus Hacker, Markus Mitterhauser, Ivo Rausch

“…Abstract Aim The combination of positron emission tomography (PET) and magnetic resonance imaging (MRI) provides an innovation leap in the use of fertilized chicken eggs (in ovo model) in preclinical imaging as PET/MRI enables the investigation of the chick embryonal organ-specific distribution of PET-tracers. However, hybrid PET/MRI inheres technical challenges in quantitative in ovo PET such as attenuation correction (AC) for the object as well as for additional hardware parts present in the PET field-of-view, which potentially contribute to quantification biases in the PET images if not accounted for. …”
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CircTTC13 promotes sorafenib resistance in hepatocellular carcinoma through the inhibition of ferroptosis by targeting the miR-513a-5p/SLC7A11 axis by Ying Zhang, Ruiwei Yao, Mingyi Li, Chongkai Fang, Kunliang Feng, Xiuru Chen, Jinan Wang, Rui Luo, Hanqian Shi, Xinqiu Chen, Xilin Zhao, Hanlin Huang, Shuwei Liu, Bing Yin, Chong Zhong

“…In silico analysis, luciferase assays, and fluorescence in situ hybridization collectively demonstrated that circTTC13 directly targets and reduces miR-513a-5p expression, which in turn leads to the upregulation of the negative ferroptosis regulator SLC7A11. …”
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CeRNA profiling and the role in regulating gonadal development in gold pompano by Changcan Yang, Yijun Zhao, Wendong Zhao, Hai Huang, Quanqi Zhang, Jinxiang Liu

“…Dual-luciferase gene reporter system and fluorescence in situ hybridization analyses preliminary verified the regulation relationship between Rspo1 and oni-let-7d-1-p3, as well as lnc-TGFβR and oni-let-7d-1-p3 in the cytoplasm of sertoli cells. …”
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A study of the influence of the T2DL.2DS-2SS translocation and the 5S(5D) substitution from <i>Aegilops speltoides</i> on breeding-valuable traits of common wheat by R. O. Davoyan, I. V. Bebykina, E. R. Davoyan, A. N. Zinchenko, Y. S. Zubanova, D. M. Boldakov, V. I. Basov, E. D. Badaeva, I. G. Adonina, E. A. Salina

“…This research is aimed at studying 14 introgression lines with the T2DL.2DS-2SS translocation and the 5S(5D) substitution from Aegilops speltoides obtained from crossing common wheat varieties (Aurora, Krasnodarskaya 99, Nika Kubani) with the genome-substituted form Avrodes (BBAASS). Hybrid lines with different combinations of T2DL.2DS-2SS and T1BL.1RS translocations and 5S(5D) substitution were characterized by resistance to leaf and yellow rusts, productivity components and technological qualities of grain. …”
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Identification and functional characterization of AsWRKY9, a WRKY transcription factor modulating alliin biosynthesis in garlic (Allium sativum L.) by Jiaying Wu, Min Li, Wanni Wang, Yiren Su, Jie Li, Jiaxin Gong, Xianfeng Meng, Chenyuan Lin, Qiantong Zhang, Yanyan Yang, Chunyan Xu, Limei Zeng, Jihong Jiang, Xuqin Yang

“…The promoter sequence of AsFMO1 was then obtained using genomee walking. Yeast one-hybrid (Y1H) and dual-luciferase assays (LUC) confirmed that AsWRKY9 interact with the AsFMO1 promoter. …”
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Identification and expression analysis of the heat shock proteins Hsp70, Hsp90, and Hsp90b in Litopenaeus vannamei under low-temperature stress by Min Peng, Hui Wang, Shuiping Wen, Zheng Liang, Zheng Huang, Bin Zhang, Tianchong Chen, Qingyun Liu, Qiangyong Li, Yuan Meng, Yuliu Huang, Chunling Yang, Digang Zeng, Manyuan Li, Weilin Zhu, Yongzhen Zhao

“…Fluorescence in situ hybridization revealed widespread expression of hsp70, hsp90, and hsp90b in the nucleus and cytoplasm of hepatopancreatic tubular cells. …”
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Impact of mother’s own milk expression practices and processing treatments on infant health and growth outcomes: a systematic review protocol by Daniel Klotz, Carolyn Smith, Agnieszka Bzikowska-Jura, Marzia Giribaldi, Tanya Cassidy, Laura Cavallarin, Serena Gandino, Karolina Karcz, Chiara Peila, Bartłomiej Walczak, Aleksandra Wesolowska

“…The present protocol was designed to gather the evidence on the effect that the expression method, the hygiene regimen and setting and any processing used on expressed MOM can have on the clinical outcomes of recipient infants.Methods This systematic review will follow the methodological recommendations of the Cochrane Collaboration, in accordance with WHO recommendations and Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. We will apply a hybrid search strategy, by combining structured database search with predefined snowballing searches. …”
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