Association of bud and anther morphology with developmental stages of the male gametophyte of melon (Cucumis melo L.) by M. L. Nguyen, T.N.B.T. Huyen, D. M. Trinh, A. V. Voronina

“…The paper presents the results of research estimating the correlations between the length and diameter of the f lower buds, the length of the visual part of the corolla, the length of the anthers and the development stages of the male gametophyte in the F1 hybrid of the Kim Hong Ngoc melon. The strongest correlation (CC = 0.885) was found for the f lower bed diameter and a strong correlation (CC = 0.880), for the bud length. …”
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Using Blockchain in the Registration and Authentication of a Carpooling Application: From Review to Proposal by Lina Sofía Cardona Martínez, Cesar Andrés Sandoval Muñoz, Ricardo Salazar-Cabrera, Álvaro Pachón de la Cruz, Juan Manuel Madrid Molina

“…Improvements in road infrastructure, technological advancements at traffic light intersections, and the adoption of electric or hybrid vehicles are insufficient to resolve these issues. …”
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Inheritance of effective juvenile leaf rust resistance in six accessions of Aegilops speltoides Tausch by M. A. Kolesova, L. G. Tyryshkin

“…The absence of susceptible plants in hybrid populations from the crosses between resistant accessions testified to a tight linkage or, more likely, the identity of their genes associated with resistance. …”
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SpikeDroidDB: AN INFORMATION SYSTEM FOR ANNOTATION OF MORPHOMETRIC CHARACTERISTICS OF WHEAT SPIKE by M. A. Genaev, E. G. Komyshev, Fu Hao, V. S. Koval, N. P. Goncharov, D. A. Afonnikov

“…We used SpikeDroidDB for the digitization and annotation of a collection of ears of F2 hybrids from crosses between the Australian cultivar of common wheat Triple Dirk and accession KU506 of Chinese wheat Triticum yunnanense. …”
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Evaluating GRU Algorithm and Double Moving Average for Predicting USDT Prices: A Case Study 2017-2024 by RAHMAT, Munirul ula, Zara Yunizar

“…However, GRU’s lower RMSE (8.531 vs. 8.715 for DMA) indicates better adaptability to sudden price fluctuations, making it more responsive to volatile markets. A hybrid approach combining GRU and DMA reveals their complementary strengths—DMA’s minimal bias (-0.0013% MPE) supports stable trend analysis, while GRU’s slight positive bias (0.0286% MPE) captures short-term fluctuations. …”
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CircSPG21 ameliorates oxidative stress-induced senescence in nucleus pulposus-derived mesenchymal stem cells and mitigates intervertebral disc degeneration through the miR-217/SIRT... by Yongbo Zhang, Sheng Yang, Xuan You, Zhengguang Li, Liuyang chen, Rui Dai, Hua Sun, Liang Zhang

“…Interactions among circSPG21, miR-217, and the NAD-dependent protein deacetylase sirtuin-1 (SIRT1) were validated through dual-luciferase assays, RNA fluorescence in situ hybridization and RNA immune precipitation. β-Gal staining, EdU staining, Western blotting, JC-1 assays, cell cycle analysis, and quantitative reverse transcription PCR (RT‒qPCR) were used to examine the functions of these molecules in TBHP-induced senescent NPMSCs. …”
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Historical biogeography and systematics of yellow-bellied toads (Bombina variegata), with the description of a new subspecies from the Balkans by Christophe Dufresnes, Simeon Lukanov, Sven Gippner, Johanna Ambu, Ilias Strachinis, Dragan Arsovski, Benjamin Monod-Broca, Hugo Cayuela, Petros Lymberakis, Daniele Canestrelli, Dan Cogălniceanu, Nikolay A. Poyarkov, Spartak N. Litvinchuk, Tomasz Suchan, Mathieu Denoël, Daniel Jablonski

“…As it reached the Dinarides during the Late Pleistocene, B. v. variegata potentially hybridized with B. v. scabra and captured its mitochondrial DNA, which resulted in a massive cyto-nuclear discordance across all northwestern European populations. …”
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Transcriptomic signatures of cold acclimated adipocytes reveal CXCL12 as a Brown autocrine and paracrine chemokine by Marina Agueda-Oyarzabal, Marie S. Isidor, Kaja Plucińska, Lars R. Ingerslev, Oksana Dmytriyeva, Patricia S.S. Petersen, Sara Laftih, Axel B. Pontoppidan, Jo B. Henningsen, Kaja Rupar, Erin L. Brown, Thue W. Schwartz, Romain Barrès, Zachary Gerhart-Hines, Camilla C. Schéele, Brice Emanuelli

“…Cxcl12 mRNA expression analysis by RT-qPCR and fluorescence in situ hybridization specified Cxcl12 distribution in various cell types, and indicated its enrichment in cold-acclimated brown adipocytes. …”
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Persistence of Hepatitis C RNA in Liver Allografts Is Associated with Histologic Progression Independent of Serologic Viral Clearance by M. Ghabril, R. C. Dickson, M. Krishna, R. Lloyd, J. Aranda-Michel, A. Keaveny, R. Satyanarayana, H. Bonatti

“…HCV RNA can be detected in liver tissue by in situ hybridization (ISH). Aim. To determine if HCV nondetectability in liver allografts by ISH can predict SVR in patients who cleared virus serologically on treatment. …”
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Application of 2D polymerase chain reaction for single-tube detection of high-risk human papillomaviruses by Weifeng Wang, Min Jiang, Ying Liu, Xuan Wu, Yan Chen, Xiaoyun Zhang, Weiwei Liu

“…A total of 294 cervical exfoliated cell samples were tested using 2D PCR and flow fluorescence hybridization method. The overall concordance between the two detection methods was 96.17% (Kappa = 0.910).ConclusionThe 2D PCR method, which integrates asymmetric PCR amplification with melting curve analysis, has the capacity to detect 11 types of HR-HPVs across three channels. …”
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The Exocyst Subunits EqSec5 and EqSec6 Promote Powdery Mildew Fungus Growth and Pathogenicity by Jinyao Yin, Xuehuan Zhu, Yalong Chen, Yanyang Lv, Jiaxin Shan, Yuhan Liu, Wenbo Liu, Weiguo Miao, Xiao Li

“…The silencing of <i>EqSec5</i> or <i>EqSec6</i> also inhibited the secretion of the effector protein EqIsc1, interrupting plant salicylic acid biosynthesis. Yeast two-hybrid and gene overexpression assays suggested that EqSec5 and EqSec6 interact with each other and can complement each other’s function during host infection. …”
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Challenges and Prospects of Digital Core-Reconstruction Research by Linqi Zhu, Chong Zhang, Chaomo Zhang, Xueqing Zhou, Zhansong Zhang, Xin Nie, Weinan Liu, Boyuan Zhu

“…Additionally, 2D slice-based digital core stochastic reconstruction techniques are classified into four types: a stochastic reconstruction method based on 2D slice mathematical-feature statistical constraints, a stochastic reconstruction method based on statistical constraints that are related to 2D slice morphological characteristics, a physics process-based stochastic reconstruction method, and a hybrid stochastic reconstruction method. The progress related to these various stochastic reconstruction methods, the characteristics of constructed 3D digital cores, and the potential of these methods are analysed and discussed in detail. …”
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Clinical characteristics, metastasis patterns, and treatment outcomes of HER2-low breast cancer by Maria Elena Lacruz, Saskia Thies, Andrea Schmidt-Pokrzywniak, Ian Wittenberg, Tobias Engler, Fabian Reinwald, Monika Klinkhammer-Schalke, Sylke Ruth Zeissig, Bianca Franke, Kerstin Weitmann, Atanas Ignatov

“…HER2 status was determined using immunohistochemistry and fluorescence in situ hybridization results, and patients were classified as HER2-positive, HER2-low or HER2-zero. …”
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Long-term results of antiviral therapy in patients with chronic hepatitis C with sustained virologic response by T. M. Ignatova, T. N. Lopatkina, V. P. Chulanov, Ye. V. Gaydasheva, S. N. Batskikh, G. V. Mikhaylovskaya, D. T. Abdurakhmanov, S. Yu. Karpov, Ye. N. Nikulkina, O. A. Chernova

“…In 114 patients RNA HCV and DNA HBV in blood serum and peripheral mononuclear blood cells were studied by polymerase chain reaction (PCR) with fluorescent hybridization detection in «real time» mode (sensitivity of 10 IU/ml for HCV and 5 IU/ml for HBV).Results. …”
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Digital Twin-Empowered Green Mobility Management in Next-Gen Transportation Networks by Kubra Duran, Lal Verda Cakir, Achille Fonzone, Trung Q. Duong, Berk Canberk

“…We note DTER achieves 19&#x0025; more successful right-time data capturing, with 30&#x0025; reduced query time. Moreover, our hybrid implementation of intelligent algorithms for emission prediction resulted in higher accuracy when compared to baselines. …”
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Morphological Neuroimaging Biomarkers for Tinnitus: Evidence Obtained by Applying Machine Learning by Yawen Liu, Haijun Niu, Jianming Zhu, Pengfei Zhao, Hongxia Yin, Heyu Ding, Shusheng Gong, Zhenghan Yang, Han Lv, Zhenchang Wang

“…From this feature pool, a hybrid feature selection algorithm combining the F-score and sequential forward floating selection (SFFS) methods was performed to select features. …”
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Novel Genomic Aberrations in Testicular Germ Cell Tumors by Array-CGH, and Associated Gene Expression Changes by Rolf I. Skotheim, Reija Autio, Guro E. Lind, Sigrid M. Kraggerud, Peter W. Andrews, Outi Monni, Olli Kallioniemi, Ragnhild A. Lothe

“…We analyzed 17 TGCTs, three precursor lesions, and the embryonal carcinoma cell lines, NTERA2 and 2102Ep, by comparative genomic hybridization microarrays (array-CGH), and integrated the data with transcriptome profiles of the same samples. …”
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Proximal bronchiolar adenoma with malignant transformation to invasive mucinous adenocarcinoma with 4 years follow-up: a case report and literature review by Yuan-Hui Yang, Ke Yin, Jia-Qi Xu, Xiao-Ying Xu, Jun-Lei Zhang, Ji-Xuan Liu, Xin-Zhi Feng, Xiao-Yan Lin, Xiao-Yan Lin

“…Regular postoperative follow-up showed no recurrence or metastasis. Hybridization Capture-based next-generation sequencing (NGS) analysis detected driver gene mutations in Kirsten Rat Sarcoma viral oncogene homolog (KRAS) and Cyclin-Dependent Kinases (CDK) 6 in the case, thereby inferring the malignant transformation of BA into IMA.ConclusionIn this case, the detection of driver gene KRAS mutation and loss of continuity in the basal cell layer within the mucous glandular structures of the nodule suggests the malignant transformation of BA into IMA, inferring the malignant potential of BA.…”
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Epidemiology of bacterial biofilms on polyps and normal tissues in a screening colonoscopy cohort by Julia L. Drewes, Samara B. Rifkin, Madison McMann, Sara Glass, Emma Spence, Caroline R. Wensel, Abby L. Geis, Courtney Stevens, Joell J. Gills, Hao Wang, Linda M. Hylind, Gerard Mullin, David Kafonek, David Cromwell, Louis La Luna, Francis M. Giardiello, Cynthia L. Sears

“…Here we evaluated potential procedural and demographic risk factors associated with biofilm status using a cross-sectional observational cohort.Methods Histologically normal colonic biopsies from 2,051 individuals undergoing screening colonoscopy were evaluated for biofilm status using fluorescence in situ hybridization with oligonucleotide probes targeting bacterial 16S rRNA. …”
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A machine learning model using the snapshot ensemble approach for soil respiration prediction in an experimental Oak Forest by S.N. Ferdous, J.P. Ahire, R. Bergman, L. Xin, E. Blanc-Betes, Z. Zhang, J. Wang

“…Here, we developed a novel ML model that integrated a hybrid Prophet-ANN and snapshot ensemble approach. …”
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