Comprehensive Analysis of Differentially Expressed miRNAs and mRNAs Reveals That miR-181a-5p Plays a Key Role in Diabetic Dermal Fibroblasts by Peng Liu, Yi Zhu, Qin Li, Biao Cheng

“…A diabetic nonhealing wound causes heavy economic burden and compromised quality of life in patients. The human dermal fibroblast (HDF), which is an important kind of effector cell in the wound healing process, represents different biological behaviors in the normal and diabetic skins. …”
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Fibrosis in PCLS: comparing TGF-β and fibrotic cocktail by Carlos Machahua, Thomas M. Marti, Patrick Dorn, Manuela Funke-Chambour

“…Our aim was to compare the pro-fibrotic effects of TGF-β1 with FC in human precision-cut lung slices (PCLS). Methods PCLS from “healthy” lung tissue of cancer patients undergoing surgery (n = 7) were incubated with TGF-β1, FC or control for 72 h. …”
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Serial Paediatrics Omics Tracking in Myalgic Encephalomyelitis (SPOT-ME): protocol paper for a multidisciplinary, observational study of clinical and biological markers of paediatr... by Adam Scheinberg, Natalie Thomas, Katherine Huang, Paul R Gooley, Elisha K Josev, Tracey Chau, Darcy Tantanis, Sarah J Knight, Christopher W Armstrong

“…The outcomes of this research have the potential to identify biomarker signatures, aid in understanding the underlying mechanisms, and ultimately, improve the lives of children with ME/CFS.Ethics and dissemination This project was approved by the Royal Children’s Hospital’s Human Research Ethics Committee (HREC 74175). Findings from this study will be disseminated through peer-reviewed journal publications and presentations at relevant conferences. …”
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Carthamin yellow-loaded glycyrrhetinic acid liposomes alleviate interstitial fibrosis in diabetic nephropathy by Yifei Wang, Wenfang He, Peiyao Ren, Li Zhao, Danna Zheng, Juan Jin

“…In vitro experiments with TGF-β1-stimulated human renal interstitial fibroblasts (hRIFs) examined the effects of CY-loaded GA liposomes on cell proliferation and the expression of fibrotic markers. …”
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Exceeding the limits of paediatric heat stress tolerance: the risk of losing a generation to climate inaction by Sulani Nyimbili, Anna Bershteyn, Alexander Azan, Oyinkansola O Babayode

“…Greenhouse gas (GHG) emissions are creating unprecedented climate-driven extreme weather, with levels of heat and humidity surpassing human physiological tolerance for heat stress. These conditions create a risk of mass casualties, with some populations particularly vulnerable due to physiological, behavioural and socioeconomic conditions (eg, lack of adequate shelter, limited healthcare infrastructure, sparse air conditioning access and electrical grid vulnerabilities). …”
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Cytotoxicity and genotoxicity of orthodontic bands after aging: an in-vitro study by Maryam Karandish, Bahar Khademi, Golnaz Ziaei, Nasrollah Erfani, Leila Moezi, Zahra Faghih

“…Abstract Background This investigation sought to evaluate cytotoxic and genotoxic effects of two different types of orthodontic bands after aging in acidic and neutral artificial saliva using human gingival fibroblast-like (HGF1-PI 1) cell lines. …”
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FTO attenuates TNF-α-induced damage of proximal tubular epithelial cells in acute pancreatitis-induced acute kidney injury via targeting AQP3 in an N6-methyladenosine-dependent man... by Xinghui Li, Qi Liang, Lu Liu, Shujun Chen, Yong Li, Yu Pu

“…Therefore, the aim of this study is to explore the association of FTO and AQP3 on proximal tubular epithelial cell damage in SAP-induced AKI.Methods An in-vitro AKI model was established in human proximal tubular epithelial cells (PTECs) HK-2 via tumor necrosis factor-α (TNF-α) induction (20 ng/mL), after which FTO and AQP3 expression was manipulated and quantified by quantitative real-time PCR and Western blotting. …”
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Health impacts and economic costs of residential fires (RESFIRES study): protocol for a population-based cohort study using linked administrative data by Lara A Harvey, Nargess Ghassempour, Mark Whybro, W Kathy Tannous

“…Costing models will be constructed.Ethics and dissemination Ethical approval for this study has been obtained from the NSW Population and Health Service Research Ethics Committee and Western Sydney University Human Research Ethics Committee. The study reference group comprises key stakeholders including Fire and Rescue NSW, policy agencies, health service providers and burns clinicians ensuring wide dissemination of results and translation of data to inform practice and identify areas for targeted prevention. …”
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Addition of PARP1-inhibition enhances chemoradiotherapy and thermoradiotherapy when treating cervical cancer in an in vivo mouse model by Marloes IJff, Xionge Mei, Enzo M. Scutigliani, Hans M. Rodermond, Gregor G. W. van Bochove, Przemek M. Krawczyk, Nicolaas A. P. Franken, Lukas J. A. Stalpers, Johannes Crezee, Arlene L. Oei

“…For the in vivo study, human cervical cancer (SiHa) cells were injected in the right hind leg of athymic nude mice. …”
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Identification and Functional Characterization of P159L Mutation in in a Family with Maturity-Onset Diabetes of the Young 5 (MODY5) by Eun Ky Kim, Ji Seon Lee, Hae Il Cheong, Sung Soo Chung, Soo Heon Kwak, Kyong Soo Park

“…The wild-type and mutant HNF1B constructs were transfected into COS-7 cells in the presence of the promoter sequence of human glucose transporter type 2 (GLUT2). The luciferase reporter assay revealed that P159L HNF1B had decreased transcriptional activity compared to wild-type (p < 0.05). …”
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Individual participant data meta-analysis of trials comparing frozen versus fresh embryo transfer strategy (INFORM): a protocol by Lan N Vuong, Rui Wang, Ben W Mol, Rong Li, Karin Hammarberg, Kate Brian, Yun Sun, Pollyanna Hardy, Anja Pinborg, Siladitya Bhattacharya, Sacha Stormlund, David J McLernon, Zi-Jiang Chen, Richard S Legro, Carlos Simon, Shimona Lai, Marian G Showell, Daimin Wei, Ze Wang, Keliang Wu, Xavier Santamaría, Christophe Blockeel, Femke Mol, Anna P Ferraretti, Bruce S Shapiro, Forest C Garner, Christos A Venetis, Abha Maheshwari, Gwenda Burns

“…We will harmonise the IPD and perform a two-stage meta-analysis and examine treatment–covariate interactions for important baseline characteristics.Ethics and dissemination The study ethics have been granted by the Monash University Human Research Ethics Committee (Project ID: 30391). …”
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A systemic analysis of Creutzfeldt Jakob disease cases in Asia by Urwah Rasheed, Sana Khan, Minahil Khalid, Aneeqa Noor, Saima Zafar

“…Sporadic CJD, which occurs for reasons unbeknown to science (85% of known cases), Genetic or Familial CJD which is characterized by the presence of mutations in the human prion protein (PRNP) gene (10–15% cases) and Iatrogenic CJD that occurs via accidental transmission through medical and surgical procedures (1–2% cases). …”
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Metagenomic analysis of DNA viruses from posttransplant lymphoproliferative disorders by Vikas R. Dharnidharka, Marianna B. Ruzinova, Chun‐Cheng Chen, Priyanka Parameswaran, Harry O'Gorman, Charles W. Goss, Hongjie Gu, Gregory A. Storch, Kristine Wylie

“…In univariable analysis, death within 5 years of PTLD diagnosis was associated with anellovirus (P = 0.037) and gammapapillomavirus (P = 0.036) detection by MSS, higher tissue qPCR levels of the predominant human anellovirus species torque teno virus (TTV; P = 0.016), T cell type PTLD, liver, brain or bone marrow location. …”
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Component composition of essential oil in the North American <i>Pinus</i> L. species introduced to the Southern Coast of Crimea by T. M. Sakhno, Y. V. Plugatar, O. M. Shevchuk, S. A. Feskov, I. V. Bulavin

“…Their volatiles play an important role in combating pathogenic microflora and removing harmful microorganisms from the air, thus benefiting human health. Conifers are highly effective as part of parklands and urban landscaping.Materials and methods. …”
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Investigating BLS instructors’ ability to evaluate CPR performance: focus on compression depth, rate, and recoil by Shih-Jhan Lin, Chih-Jan Chang, Shao-Chung Chu, Ying-Hsin Chang, Ming-Yuan Hong, Po-Chang Huang, Chia-Lung Kao, Chih-Hsien Chi

“…Key metrics such as chest compression speed, depth, and recoil were examined to identify the degree of consistency between human and automated evaluations. Results In this study, CPR performance was analyzed using QCPR devices and BLS instructors across metrics like speed, depth, and recoil. …”
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MEG3 Alleviated LPS-Induced Intestinal Injury in Sepsis by Modulating miR-129-5p and Surfactant Protein D by Xianjin Du, Dan Tian, Jie Wei, Chen Yan, Peng Hu, Xu Wu, Wenbin Yang

“…Subsequently, to induce the inflammatory injury of sepsis, human colorectal Caco2 cells were treated with 10 ng/ml LPS. 10 ng/ml LPS significantly inhibited Caco2 cell proliferation and increased the apoptosis. …”
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Lidocaine Shows Significant Antimicrobial Effects Against <i>Staphylococcus</i> Species: An In-Vitro Study Comparing Different Combinations of Lidocaine and Clinically Used Injecta... by Stephan Heller, Ricarda Johanna Seemann, Rainer Burgkart, Andreas Obermeier, Hermann Locher

“…Additional tests were conducted on <i>Staphylococcus epidermidis</i> and methicillin-resistant Staphylococcus aureus (MRSA), as well as on <i>S. aureus</i> in human synovial fluid. Results: Lidocaine showed a concentration-dependent antimicrobial effect, with greater inhibition at higher concentrations and lower bacterial densities. …”
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Alpha-linolenic acid-mediated epigenetic reprogramming of cervical cancer cell lines by Amrita Ulhe, Prerna Raina, Amol Chaudhary, Ruchika Kaul-Ghanekar

“…Cervical cancer, the fourth most common cancer globally and the second most prevalent cancer among women in India, is primarily caused by Human Papilloma Virus (HPV). The association of diet with cancer etiology and prevention has been well established and nutrition has been shown to regulate cancer through modulation of epigenetic markers. …”
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Protocol for the enhanced management of multimorbid patients with COPD and severe asthma: role of indoor air quality by Emili Vela, Isaac Cano, Josep Roca, Antoni Sisó-Almirall, Carme Hernandez, Ebymar Arismendi, Ramon Farré, Alba Gómez-López, María Figols, Antonio Montilla-Ibarra, Núria Sánchez-Ruano, Benigno Sánchez, Marta Sorribes, Jordi Piera-Jiménez, Jaume Benavent, Jose Fermoso, Rubèn González-Colom

“…Each cycle involves a targeted cocreation process following a mixed-methods approach with the active participation of patients, health professionals, managers and digital experts.Ethics and dissemination The Ethics Committee for Human Research at Hospital Clinic de Barcelona approved the protocol on 29 June 2023 (HCB/2023/0126). …”
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Advancements and challenges in agriculture: a comprehensive review of machine learning and IoT applications in vertical farming and controlled environment agriculture by Binoy Sasmal, Gobinda Das, Preeti Mallick, Sneha Dey, Suman Ghorai, Subrata Jana, Chiranjibe Jana

“…In VF, aeroponics consumes 98% less water than conventional farming. Human health, comfort, and productivity directly correlate with the indoor climate. …”
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