Comparative analysis of the primary structure and production of recombinant poly(ADP-ribose)polymerase 1 of long-lived Heterocephalus glaber by K. N. Naumenko, A. R. Nurislamov, K. D. Nazarov, V. S. Fishman, A. A. Popov, I. O. Petruseva, A. N. Evdokimov, O. I. Lavrik

“…In the present work, the amino acid sequence of PARP1 of the naked mole rat is compared with the amino acid sequences of orthologous proteins of other mammals. In contrast to human PARP1, 13 evolutionarily conservative amino acid substitutions in various functional domains of the protein have been identified in the amino acid sequence of naked mole rat PARP1. …”
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Hobbit: Providing Fall Detection and Prevention for the Elderly in the Real World by Markus Bajones, David Fischinger, Astrid Weiss, Daniel Wolf, Markus Vincze, Paloma de la Puente, Tobias Körtner, Markus Weninger, Konstantinos Papoutsakis, Damien Michel, Ammar Qammaz, Paschalis Panteleris, Michalis Foukarakis, Ilia Adami, Danai Ioannidi, Asterios Leonidis, Margherita Antona, Antonis Argyros, Peter Mayer, Paul Panek, Håkan Eftring, Susanne Frennert

“…Our trials show the necessity to move out into actual user homes, as only there can we encounter issues such as misinterpretation of actions during unscripted human-robot interaction.…”
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Body mass index changes and predictors among adults living with HIV/AIDS who are on anti-retroviral therapy at Chiro General Hospital, Eastern Ethiopia: a facility-based retrospect... by Sintayehu Amsalu, Tara Wilfong, Sewmehon Amsalu, Abdu Oumer, Berhe Gebremichael

“…Abstract Background Human immunodeficiency virus continues to be a major global public health issue. …”
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A murine model of Trypanosoma brucei-induced myocarditis and cardiac dysfunction by Nathan P. Crilly, Marcelle Dina Zita, Alexander K. Beaver, Polina Sysa-Shah, Aashik Bhalodia, Kathy Gabrielson, Luigi Adamo, Monica R. Mugnier

“…ABSTRACT Trypanosoma brucei is a protozoan parasite that causes human and animal African trypanosomiases (HAT and AAT). …”
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Antioxidant, Anti-Tyrosinase, and Wound-Healing Capacities of Soy Protein Hydrolysates Obtained by Hydrolysis with Papaya and Cantaloupe Juices Showing Proteolytic Activity by Thi-Phuong Nguyen, Quang Thai Le, Mai Linh Thi Tran, Kim Nhung Ta, Khoa Thi Nguyen

“…Remarkably, the hydrolysates prepared from the hydrolysis with fruit juices accelerated the wound closure in human fibroblasts by estimately 1.5 times after 24 h of treatment while this property was not observed in the hydrolysate by papain. …”
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Improving the integrity and reproducibility of research that uses antibodies: a technical, data sharing, behavioral and policy challenge by M. Biddle, P. Stylianou, M. Rekas, A. Wright, J. Sousa, D. Ruddy, M. I. Stefana, K. Kmiecik, A. Bandrowski, R.A. Kahn, C. Laflamme, E. M. Krockow, H.S. Virk

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N100 Repetition Suppression Indexes Neuroplastic Defects in Clinical High Risk and Psychotic Youth by Joseph Gonzalez-Heydrich, Michelle Bosquet Enlow, Eugene D’Angelo, Larry J. Seidman, Sarah Gumlak, April Kim, Kristen A. Woodberry, Ashley Rober, Sahil Tembulkar, Kyle O’Donnell, Hesham M. Hamoda, Kara Kimball, Alexander Rotenberg, Lindsay M. Oberman, Alvaro Pascual-Leone, Matcheri S. Keshavan, Frank H. Duffy

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Evaluation of in-office tooth whitening treatment with violet LED: protocol for a randomised controlled clinical trial by Lara Jansiski Motta, Anna Carolina Ratto Tempestini Horliana, Sandra Kalil Bussadori, Maria Fernanda Setúbal Destro Rodrigues, Ana Eliza Castanho Garrini dos Santos, Marcelo Mendes Pinto, Dácio Antonio Pantano Junior, Aldo Brugnera Jr, Fátima Antonia Aparecida Zanin

“…Multivariate analysis of variance (MANOVA) and the Wilcoxon test will be used for comparing data from the PIDAQ.Ethics and dissemination This protocol has been approved by the Human Research Ethics Committee of UniversidadeNove de Julho (certificate: 2.034.518). …”
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Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture by Joo Hyun Jee, Dong Hyeon Lee, Jisu Ko, Soojung Hahn, Sang Yun Jeong, Han Kyung Kim, Enoch Park, Seon Young Choi, Sukin Jeong, Joong Woon Lee, Han-Jun Cho, Min-Soo Kwon, Jongman Yoo

“…Mouse small intestine–derived organoids, stomach-derived organoids, and human colon–derived organoids were also cultured, all of which were successfully grown in the collagen-based matrix and had similar properties compared to those cultured in Matrigel. …”
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Rare Taxa Are Key Links in Regional Antimicrobial Resistance Profiles in Dusts Across Diverse North American Regions by Paul B. L. George, Florent Rossi, Marc Veillette, Amélia Bélanger Cayouette, Samantha Leclerc, Cindy Dumais, Nathalie Turgeon, Caroline Duchaine

“…Factors positively linked to total numbers of ARGs included human and livestock populations; whereas mean annual precipitation was negatively linked to resistance gene quantities. …”
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Loss of Lkb1 cooperates with BrafV600E and ultraviolet radiation, increasing melanoma multiplicity and neural‐like dedifferentiation by Kimberley McGrail, Elena González‐Sánchez, Paula Granado‐Martínez, Roberto Orsenigo, Yuxin Ding, Berta Ferrer, Javier Hernández‐Losa, Iván Ortega, Juan Martín‐Caballero, Eva Muñoz‐Couselo, Vicente García‐Patos, Juan A. Recio

“…The mechanisms that work alongside BRAFV600E oncogene in melanoma development, in addition to ultraviolet (UV) radiation (UVR), are of great interest. Analysis of human melanoma tumors [data from The Cancer Genome Atlas (TCGA)] revealed that 50% or more of the samples expressed no or low amounts of serine/threonine protein kinase STK11 (also known as LKB1) protein. …”
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Frequency of Trypanosoma cruzi Infection in Synanthropic and Wild Rodents Captured in a Rural Community in Southeast of Mexico by Ivonne Hernández-Cortazar, Karla Cecilia Amaya Guardia, Marco Torres-Castro, Karla Acosta-Viana, Eugenia Guzmán-Marín, José Israel Chan-Pérez, Antonio Ortega-Pacheco, Roger I. Rodríguez-Vivas, Rodrigo Medina-Pinto, Matilde Jiménez-Coello

“…The active and chronic infection of T. cruzi in synanthropic or wild rodents of the rural community of southern Mexico evidences the natural infection in these reservoirs which contribute to maintaining the agent in the wild and domestic environments and can represent a risk of infection for the human population when the vector is present.…”
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Economic and relationship-strengthening intervention to reduce alcohol use in couples living with HIV in Malawi: a study protocol for a randomised controlled trial of Mlambe by Torsten B Neilands, Lynae A Darbes, James Mkandawire, Derek S Brown, Fred M Ssewamala, Judith A Hahn, Amy A Conroy, Nancy Mulauzi, Scott Tebbetts

“…Programmatic costs will be ascertained throughout the study and incremental cost-effectiveness ratios will be computed for each arm.Ethics and dissemination The RCT has been approved by the University of California, San Francisco (UCSF) (Human Research Protection Program; Protocol Number 23-40642), and the study has been approved by the National Health Sciences Research Committee (NHSRC; Protocol Number 24/05/4431) in Malawi. …”
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Optimization of temperature parameters for screening unexpected antibodies in Rh system by manual polybrene test by ZOU Xin, CHEN Minjie, MA Sifei, YANG Hongmei

“…[Methods] The reaction of IgG human anti-D antibody with different dilutions (undiluted, 1∶2, 1∶4, 1∶8, 1∶16, 1∶32，1∶64) with D antigen-positive red blood cells was detected by manual polybrene test (MPT). …”
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Date pomace polysaccharides-capped selenium nanoparticles: Biosynthesis, optimization, physicochemical characterization, biological activities, stability and gut microbiota modulat... by Gafar Babatunde Bamigbade, Athira Subhash, Mohammed Abdin, Hebah Jarusheh, Basim Abu-Jdayil, Shao-Quan Liu, Giovanni Palmisano, Abdelmoneim Ali, Afaf Kamal Eldin, Mutamed Ayyash

“…The MPS-NPS characteristics, digestibility and impact on human gut microbiota were investigated. The MPS-NPS were well-stabilized, spherical particles averaging 65.4 nm in size, with PDI (0.032), zeta potential (–21.07 mV) and notable antioxidant activity (82.7 % DPPH, 68.9 % ABTS, 491.0 µg/mL FRAP, and 961.2 µg/mL TAC at 100 mg/L). …”
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In Vitro Expansion of Bone Marrow Derived Mesenchymal Stem Cells Alters DNA Double Strand Break Repair of Etoposide Induced DNA Damage by Ian Hare, Marieta Gencheva, Rebecca Evans, James Fortney, Debbie Piktel, Jeffrey A. Vos, David Howell, Laura F. Gibson

“…To investigate the response to DSB stress with passage in vitro, primary human MSCs were exposed to etoposide (VP16) at various passages with subsequent evaluation of cellular damage responses and DNA repair. …”
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Microbial assisted alleviation of nickel toxicity in plants: A review by Xinyu Mao, Bilal Ahmad, Sabir Hussain, Farrukh Azeem, Muhammad Waseem, Yousef Alhaj Hamoud, Hiba Shaghaleh, Amany H.A. Abeed, Muhammad Rizwan, Jean Wan Hong Yong

“…Conversely, when nickel is present in high concentration, it is considered as a toxic substance. Excessive human nickel exposure occurs through ingestion, inhalation, and skin contact, ultimately leading to respiratory, cardiovascular, and chronic kidney diseases. …”
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Analysis of serum levels and DNA methylation of fibroblast growth factor 21 using peripheral blood-derived genomes in patients with obesity by Hiroyuki Shinozaki, Shiori Kawai, Mami Gamo-Kawasaki, Ayano Takei, Kyoko Tsujikado, Kazunori Fukuda, Mototaka Yamauchi, Kenji Hara, Takafumi Tsuchiya, Kohzo Takebayashi, Koshi Hashimoto

“…In the present study, we analyzed the DNA methylation state of the FGF21 gene (FGF21) in obese patients using genomic DNA extracted from human monocytes and macrophages and investigated the pathophysiological significance of the FGF21 expression response to pemafibrate (PM), a PPARα ligand. …”
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The challenges of evidence-based prehabilitation in a real-life context for patients preparing for colorectal surgery—a cohort study and multiple case analysis by A. D. Talen, N. L. U. van Meeteren, J. A. Barten, I. Pereboom, W. P. Krijnen, H. Jager-Wittenaar, B. C. Bongers, G. van der Sluis

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Study protocol: an observational study of distress, immune function and persistent pain in HIV by John A Joska, Mark R Hutchinson, Maia Lesosky, Victoria J Madden, Ncumisa Msolo, Luyanduthando Mqadi, Gillian J Bedwell, Jonathan Grant Peter, Romy Parker, Andrew Schrepf, Robert R Edwards

“…The study has been approved by the Human Research Ethics Committee of the University of Cape Town (approval number: 764/2019) and the City of Cape Town (ref: 24699).Trial registration number NCT04757987.…”
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