Enhanced membrane protein production in HEK293T cells via ATF4 gene knockout: A CRISPR-Cas9 mediated approach by Byung-Jo Choi, Ba Reum Kim, Ho Joong Choi, Ok-Hee Kim, Say-June Kim

“… HEK293T cells are extensively utilized for therapeutic protein production due to their human origin, which enables accurate post-translational modifications. …”
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Effect of amisulpride on the expression of serotonin receptors, neurotrophic factor BDNF and its receptors in mice with overexpression of the aggregation-prone [R406W] mutant tau p... by E. M. Kondaurova, A. A. Komarova, T. V. Ilchibaeva, A. Ya. Rodnyy, E. A. Zalivina, V. S. Naumenko

“…In this study, we investigated the effects of amisulpride (5-HT7R inverse agonist) in C57BL/6J mice with experimentally induced expression of the gene encoding the aggregation-prone human Tau[R406W] protein in the prefrontal cortex. …”
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Effects of E-Cigarette Liquid Ratios on the Gravimetric Filter Correction Factors and Real-Time Measurements by Austin Close, Jane Blackerby, Heather Tunnell, Jack Pender, Eric Soule, Sinan Sousan

“…Abstract Electronic cigarettes (ECIGs) generate high concentrations of particulate matter (PM), impacting the air quality inhaled by humans through secondhand exposure. ECIG liquids are available commercially and some users create their own “do-it-yourself” liquids, and these liquids often vary in the amounts of their chemical ingredients, including propylene glycol (PG) and vegetable glycerin (VG). …”
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Development of a Multi-Sensor Mobile Device for Urban Air Quality Monitoring at the Street Corner: The SMILE Project by Jonas Pellegrino, Hassen Aziza, Mathieu Guerin, Pascal Taranto, Wenceslas Rahajandraibe, Blaise Ravelo

“…Air pollution is a critical contributor to the global climate change crisis and poses severe threats to human health worldwide. In this context, this paper introduces an innovative multi-sensor air quality monitoring (AQM) device designed to address the critical challenge of atmospheric pollution in urban environments. …”
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Progression of ampC amplification during de novo amoxicillin resistance development in E. coli by Luyuan Nong, Martijs Jonker, Wim de Leeuw, Meike T. Wortel, Benno ter Kuile

“…ABSTRACT Beta-lactam antibiotics are the most applied antimicrobials in human and veterinarian health care. Hence, beta-lactam resistance is a major health problem. …”
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Klasifikasi Ekspresi Wajah Menggunakan Covolutional Neural Network by Ahmad Taufiq Akbar, Shoffan Saifullah, Hari Prapcoyo

“…Abstract Facial expression recognition is a significant challenge in image processing and human-computer interaction due to its inherent complexity and variability. …”
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Maternal singing and speech have beneficial effects on preterm infant’s general movements at term equivalent age and at 3 months: an RCT by Manuela Filippa, Gianluca Filippa, Elisa Della Casa, Alberto Berardi, Odoardo Picciolini, Sara Chiara Meloni, Clara Lunardi, Alessandra Cecchi, Alessandra Sansavini, Luigi Corvaglia, Luigi Corvaglia, Didier Grandjean, EVC Group, Fabrizio Ferrari

“…To avoid influencing outcomes, future research should control for differences in maternal engagement outside of the intervention.ConclusionLive maternal singing and speaking are fundamental human practices that, in this study, enhanced preterm infants’ general movements and potentially supported their neurobehavioral development. …”
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Spatiotemporal transcriptome and scRNA sequencing analysis reveals that IKFZ1‐mediated microglia underlies a therapy for intracerebral hemorrhage by Wenqiao Qiu, Jie Tian, Gao Mou, Lili Guo, Tao Xu, Wei Liu, Jianwei Zhu, Yi Zhang, Xiaolin Hou, Yao Xie, Huan Xiong, Xinda Li, Yangyang Wang, Mingjun Gao, Anguo Wu, Longyi Chen, Jie Mei, Lulin Huang, Ruxiang Xu

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miR-199a-5p Exacerbated Intestinal Barrier Dysfunction through Inhibiting Surfactant Protein D and Activating NF-κB Pathway in Sepsis by Xianjin Du, Dan Tian, Jie Wei, Chen Yan, Peng Hu, Xu Wu, Wenbin Yang, Zhanyong Zhu

“…Multiple investigations indicate that miR-199a-5p participates in different human diseases. Nevertheless, little is known on the roles of miR-199a-5p in sepsis. …”
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A Comparative Analysis of Spatial Resolution Sentinel-2 and Pleiades Imagery for Mapping Urban Tree Species by Fabio Recanatesi, Antonietta De Santis, Lorenzo Gatti, Alessio Patriarca, Eros Caputi, Giulia Mancini, Chiara Iavarone, Carlo Maria Rossi, Gabriele Delogu, Miriam Perretta, Lorenzo Boccia, Maria Nicolina Ripa

“…Urbanization poses significant challenges to ecosystems, resources, and human well-being, necessitating sustainable planning. …”
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Approaches to implementing and financing primary health care in Kenya: a case of seven counties by Janette Karimi, Salim Hussien, Elizabeth Wangia, Mercy-Irene Kimani, Mohamud Mohamed, Melissa Wanda, Rosemarie Muganda, Rachel Ndirangu, Daniel Mwai, Mercy Wanjala, Fadhila Richter

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Pengembangan Aplikasi Fokus Timer Dengan Tracking Penggunaan Smartphone Menggunakan Pendekatan Gamifikasi by Dava Mohammad Hamka, Lutfi Fanani, Adam Hendra Brata

“…Abstract Smartphones have become an inseparable part of human life. However, excessive use of smartphones can have negative impacts and can harm users such as decreased cognitive function and addiction which causes users to fail to focus. …”
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Exploring Methacrylated Gellan Gum 3D Bioprinted Patches Loaded with Tannic Acid or L-Ascorbic Acid as Potential Platform for Wound Dressing Application by Federica Scalia, Alessandra Maria Vitale, Domiziana Picone, Noemi De Cesare, Maria Swiontek Brzezinska, Beata Kaczmarek-Szczepanska, Alfredo Ronca, Barbara Zavan, Fabio Bucchieri, Marta Anna Szychlinska, Ugo D’Amora

“…The cytocompatibility of the patches was demonstrated by direct and indirect tests on human dermal fibroblasts (HDF) as per ISO 10993. …”
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Novel Fast-Setting and Mechanically-Strong Calcium Phosphate Pulp-Capping Cement with Metformin Release to Enhance Dental Pulp Stem Cells by Mohammad Alenizy, Abdullah Alhussein, Nader Almutairi, Ibrahim Ba-Armah, Tao Ma, Suping Wang, Nageswara R. Pilli, Maureen A. Kane, Michael D. Weir, Abraham Schneider, Hockin H. K. Xu

“…This study aimed to: (1) develop a calcium phosphate cement with chitosan (CPCC) with improved physio-mechanical properties; (2) incorporate metformin (CPCC-Met) to assess release; and (3) evaluate human dental pulp stem cells (hDPSCs) response. CPCC was mixed at different powder-to-liquid ratios to evaluate physio-mechanical properties compared to MTA. …”
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Assessing costs and constraints of forest residue disposal by pile burning by Jake Barker, Jake Barker, Jimmy Voorhis, Sinéad M. Crotty

“…In addition, pile burning on these National Forests annually emits >1.7 million MT CO2, 61,515 MT of carbon monoxide, 3,823 MT of methane, and 3,211 MT of non-methane hydrocarbons. Given the economic, human health, and climate implications of current pile burning practice, removing residues as feedstocks for carbon-negative utilizations is recommended as a near-term priority. …”
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Whole-genome sequencing-based characterization of Salmonella enterica Serovar Enteritidis and Kentucky isolated from laying hens in northwest of Iran, 2022–2023 by Shirin Vakili, Mehri Haeili, Adel Feizi, Kiarash Moghaddasi, Maryam Omrani, Arash Ghodousi, Daniela Maria Cirillo

“…Abstract Background The transmission of Salmonella spp. to human through the consumption of contaminated food products of animal origin, mainly poultry is a significant global public health concern. …”
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Needs of older adults in Kazakhstan: analysis and psychometric properties of the localized version of the EASYCare standard 2010 instrument by Kerbez Kimatova, Lyudmila Yermukhanova, Dorota Talarska, Marzena Dworacka, Gulnar Sultanova, Gulzat Sarsenbayeva, Yerlan Bazargaliyev, Perizat Aitmaganbet, Aleksandra Suwalska, Katarzyna Wieczorowska-Tobis, Katarzyna Wieczorowska-Tobis, Ian Philp, Slawomir Tobis

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Segmentasi Citra Wajah dengan Implementasi Adaptif Threshold- Integral Image by Maryam Ummul Habibah, Muchamad Kurniawan

“…This study is intended to split the object of a human face and its background. Face images that will be used later in indoor and outdoor with an image resolution of 300x400 pixels. …”
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Development of a Novel Co-Amorphous Curcumin and L-Arginine (1:2): Structural Characterization, Biological Activity and Pharmacokinetics by Jose Antonio Mancillas-Quiroz, Miriam del Carmen Carrasco-Portugal, Karina Mondragón-Vásquez, Juan Carlos Huerta-Cruz, Juan Rodríguez-Silverio, Leyanis Rodríguez-Vera, Juan Gerardo Reyes-García, Francisco Javier Flores-Murrieta, Jorge Guillermo Domínguez-Chávez, Héctor Isaac Rocha-González

“…<b>Conclusions</b>: Results suggest the possible use of CAC12 to treat inflammatory pain disorders in human beings.…”
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Role of Fcγ receptors in HER2-targeted breast cancer therapy by Hope S Rugo, Antonino Musolino, William J Gradishar, Jeffrey L Nordstrom, Edwin P Rock, Fernanda Arnaldez, Mark D Pegram

“…Several therapeutic monoclonal antibodies (mAbs), including those targeting epidermal growth factor receptor, human epidermal growth factor receptor 2 (HER2), and CD20, mediate fragment crystallizable gamma receptor (FcγR)–dependent activities as part of their mechanism of action. …”
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