Stage-dependent changes in culture medium osmolality promote porcine oocyte maturation in vitro by Lixiang Liu, Lixiang Liu, Yu Tang, Yu Tang, Jing Shao, Jing Shao, Bingfeng Fan, Bingfeng Fan, Yifeng Yang, Yifeng Yang, Ying Zhang, Ying Zhang, Xiangyuan Zhao, Xiangyuan Zhao, Hailong Xue, Hailong Xue, Huimin Sun, Huimin Sun, Xulin Zhang, Xulin Zhang, Yushi Zhang, Yushi Zhang, Baozeng Xu, Baozeng Xu

“…In the presence of 1 mM glycine, we adjusted the osmolality of the culture medium from low to high (290 mOsM for the first 22 h and 320 mOsM thereafter) by adding raffinose, which cannot be used by animal cells, in a culture stage-dependent manner.ResultsStage-dependent adjustment of simplified medium PZM-3 osmolarity (290 mOsM for the first 22 h and 320 mOsM thereafter) in the presence of 1 mM glycine significantly improved the quality of porcine oocyte maturation in vitro, manifested by the oocyte maturation rate, functional mitochondrial distribution and activity, the transcript levels of glycolysis genes in granulosa cells, and subsequent embryonic developmental ability and ROS levels.ConclusionOur study demonstrates that optimizing cell volume regulation can further enhance the developmental potential of oocytes cultured in vitro.…”
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Biology and Biotechnology of Follicle Development by Gustavo Adolfo Palma, Martin Eduardo Argañaraz, Antonio Daniel Barrera, Daniela Rodler, Adrian Ángel Mutto, Fred Sinowatz

“…Thecal factors promote granulosa cell proliferation and suppress granulosa cell apoptosis. …”
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Establishment and Mechanism Study of a Primary Ovarian Insufficiency Mouse Model Using Lipopolysaccharide by Si-Ji Lv, Shu-Hui Hou, Lei Gan, Jing Sun

“…Also, LPS promoted granulosa cell apoptosis and ovarian fibrosis in mice. …”
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Chaihu Shugan San and Zuogui Yin synergistically improve premature ovarian failure in a rat model via the PI3K/Akt/mTOR pathway by Fei Zhang, RuiFang Liu, ZhenLing Xu, WenJie Chen, XiYa Lu, Yang Wang

“…Key outcomes, including ovarian granulosa cell proliferation, follicular integrity, and hormonal levels (FSH and E2), were assessed. …”
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Cloning, Characterization and Expression Pattern of the Ovarian Cytochrome P450 Cyp19a1aGene in Gonadal Developmental Period of Cobaltcap Silverside Hypoatherina tsurugae by Dilip Kumar Bej

“…The upregulation of the cyp19a1a transcription factor is required for granulosa cell differentiation and ovarian maintenance. 1630 bp of cyp19a1 mRNA transcript of Hypoatherina tsurugae was cloned and sequenced. …”
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Quantitative Proteomics Analysis Reveals XDH Related with Ovarian Oxidative Stress Involved in Broodiness of Geese by Ning Zhou, Yaoyao Zhang, Youluan Jiang, Wang Gu, Shuai Zhao, Wanwipa Vongsangnak, Yang Zhang, Qi Xu, Yu Zhang

“…The results of this study showed that during the broody period, geese exhibited degraded ovarian tissues, disrupted follicular development, a thinner granulosa cell layer, and lower levels of ovarian hormones E2, P4, and AMH. …”
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