Reproducibility and Consistency of Isolation Protocols for Fibroblasts, Smooth Muscle Cells, and Epithelial Cells from the Human Vagina by Jayson Sueters, Rogier Schipperheijn, Judith Huirne, Theo Smit, Zeliha Guler

“…The best results were obtained after digestion with 0.1% collagenase-IV, where pure FB colonies formed with high cell viability. (4) Conclusions: Today, vaginoplasty is considered the gold standard for surgically creating a neovagina, despite its considerable drawbacks and limitations. …”
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Comparative evaluation of APRI and MELD scores in the prediction of complications of chronic hepatitis in patients infected with hepatitis B or C viruses in Lubumbashi, democratic... by Arsène Kabamba-Tshikongo, Adolphe Baleebenga, Bernard Kalunga-Tompa, Claude Mwamba-Mulumba, Henry Manya-Mboni, Cedrick Mutombo-Shakalenga, Pierrot Mwamba-Tshilumba, Sébastien Bontems, Géraldine Dessilly, Benoît Kabamba-Mukadi, Albert Longanga-Otshudi

“…Analytical performance of APRI and MELD scores was evaluated by using liver biopsy as gold standard.The APRI and MELD scores had a sensitivity of 100 % and a specificity of 93.5 and 88.6 %, respectively. …”
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Diagnostic Accuracy of EndoFaster® and Narrow-Band Imaging Endoscopy in Patients with Impaired Gastric Acid Secretion: A Real-Time Prospective Study by M. Cazzato, G. Esposito, G. Galli, E. Pilozzi, E. Lahner, V. D. Corleto, A. Zullo, E. Di Giulio, B. Annibale

“…EndoFaster® showed an accuracy for corpus atrophic gastritis diagnosis, compared to histopathological evaluation as gold standard, of 87.1% and a sensitivity, specificity, PPV, and NPV of 78.8%, 93.1%, 89.1%, and 85.9%, respectively. pH showed a positive correlation with the severity score of atrophy (r=0.67, 95% CI: 0.73-0.81, and p<0.0001). …”
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Comparison of handheld rebound tonometry with Goldmann applanation tonometry in children with glaucoma: a cohort study by Catey Bunce, Peng Tee Khaw, John Brookes, Maria Papadopoulos, Amanda Lewis, Poornima Rai, Renata Puertas, Annegret Hella Dahlmann-Noor, Shenille Tabasa-Lim, Ahmed El-Karmouty, Mustafa Kadhim, Nicholas Kloster Wride, Dawn Grosvenor

“…Objective To test agreement of two methods to measure intraocular pressure (IOP): rebound tonometry (RBT) and gold standard Goldmann applanation tonometry (GAT) in children with glaucoma.Design Observational prospective cohort study.Setting Tertiary paediatric glaucoma clinic at a single centre.Participants 102 individuals attending a paediatric glaucoma clinic, mean (SD) age 11.85 (3.17), of whom 53 were male.Primary and secondary outcome measures Intraocular pressure, central corneal thickness, child preference for measurement method.Results Limits of agreement for intraobserver and interobserver were, respectively, (−2.71, 2.98) mm Hg and (−5.75, 5.97) mm Hg. …”
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Impact of Nasal Anatomical Variation Subtype on Surgical Outcomes for Rhinogenic Contact Point Headache by Salvatore Lavalle, Annalisa Pace, Giuseppe Magliulo, Mario Lentini, Jerome Rene Lechien, Christian Calvo-Henriquez, Federica Maria Parisi, Giannicola Iannella, Antonino Maniaci, Daniela Messineo

“…This study provides new evidence that preoperative CT scans should be considered a non-invasive gold standard for analyzing nasal subtypes as they significantly influence surgical success, with concha bullosa associated with worse pain relief. …”
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Video Review Can Measure Surgical Robotic Skill Development in a Resident Robotic Hernia Curriculum: A Retrospective Cohort Study by Sarah B. Hays, MD, Amr I. Al Abbas, MD, Kristine Kuchta, MS, Aram Rojas, MD, Melissa Ramirez Barriga, BS, Syed Abbas Mehdi, MBBS, MPH, Stephen Haggerty, MD, H. Mason Hedberg, MD, John Linn, MD, Mark Talamonti, MD, Melissa E. Hogg, MD, MS

“…The drills were completed by 3 attending surgeons to establish gold-standard benchmarks. Results:. In total, 20 residents started the curriculum, 19 completed all IHR drills and 17 completed all VHR drills. …”
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Wetland degradation monitoring using multi-temporal remote sensing data and watershed land degradation index by I. Ridwan, S. Kadir, N. Nurlina

“…Environmental damage in the Tabunio Watershed is exacerbated by the existence of traditional gold mining and has become a concern for many parties. …”
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Leveraging Autofluorescence for Tumor Detection, Diagnosis, and Accurate Excision with Surgical Margin Assessment in Tumor Excision by Antonis Perdiou, Ramona Dumitrescu, Daniela Jumanca, Octavia Balean, Ruxandra Sava-Rosianu, Serban Talpos, Dacian Virgil Lalescu, Atena Galuscan

“…Histopathological analysis was the gold standard for margin evaluation, with margins coded as tumor-free margins (0), close (1), or infiltrated (2). …”
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Evaluation of Body Composition in Hemodialysis Thai Patients: Comparison between Two Models of Bioelectrical Impedance Analyzer and Dual-Energy X-Ray Absorptiometry by Kulapong Jayanama, Supanee Putadechakun, Praopilad Srisuwarn, Sakda Arj-Ong Vallibhakara, Prapimporn Chattranukulchai Shantavasinkul, Chanika Sritara, Surasak Kantachuvesiri, Surat Komindr

“…Dual-energy X-ray absorptiometry (DXA) is a gold standard test, but clinically limited. Bioelectrical impedance analysis (BIA) with multifrequency technique is a practical and reliable tool. …”
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Medication-focused telehealth interventions to reduce the hospital readmission rate: a systematic review by Fatemeh Emadi, Racha Dabliz, Rebekah Moles, Stephen Carter, Jenny Chen, Charu Grover, Manya Angley, Rohan A. Elliott, Deirdre Criddle, Deborah Rigby, Sepehr Shakib, Frank Sanfilippo, Charley Budgeon, Kim-Huong Nguyen, Paul Yates, Katie Phillips, Anna Packer, Linda Krogh, Simon Poon, Jonathan Penm

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Impact of viral load on sample pooling for reverse-transcription polymerase chain reaction detection-based diagnosis of coronavirus disease 2019 in Nigeria by Timan T. Eliya, Elvis E. Isere, Bassey Emmana, Chukwuebuka Ugwu, Jonathan Kushim, Precious Ishaku, Aisha E. Ibrahim, John S. Bimba

“…Reverse-transcription polymerase chain reaction (RT-PCR) remains the gold standard for COVID-19 detection, but limited testing resources caused bottlenecks in Nigeria’s response during the pandemic. …”
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Culture-independent detection of Mycobacterium tuberculosis complex DNA using targeted next generation sequencing in African buffalo (Syncerus caffer) oronasal swabs in South Afric... by Sinegugu Kholeka Mhlophe, Charlene Clarke, Giovanni Ghielmetti, Giovanni Ghielmetti, Megan Matthews, Tanya Jane Kerr, Michele Ann Miller, Wynand Johan Goosen, Wynand Johan Goosen

“…Controlling aTB in South Africa relies on mycobacterial culture as the gold standard for M. bovis confirmation, with the single intradermal comparative cervical test (SICCT) and Bovigam™ assays as validated cell-mediated immunological assays for detection. …”
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Low-Dose Ketamine Infusion for Perioperative Pain Management in Patients Undergoing Laparoscopic Gastric Bypass: A Prospective Randomized Controlled Trial by Mitchell T. Seman, Shawn H. Malan, Matthew R. Buras, Richard J. Butterfield, Kristi L. Harold, James A. Madura, David M. Rosenfeld, Andrew W. Gorlin

“…Laparoscopic Roux-en-Y gastric bypass is the gold standard surgical treatment for patients whose weight is refractory to diet and exercise. …”
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Use of Blood Lipid Indicators as a Screening Tool of Insulin Resistance among Individuals in Low-Income Country Sides of China: A Multiethnic Region Study by Yi-Zhong Yan, Jia-Ning Fan, Jia-Ming Liu, Yun-hua Hu, Jiao-Long Ma, Jia He, Heng Guo, Xiang-hui Zhang, Xin-ping Wang, Shu-gang Li, Shu-Xia Guo

“…The homeostasis model assessment was the gold standard for identifying IR. The receiver operating characteristic (ROC) curve was used to evaluate the diagnostic value, and the nomogram was utilized to analyze the predictive value. …”
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Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis? by Natalia Souza de Godoy, Marcos Luiz Alves Andrino, Regina Maia de Souza, Erika Gakiya, Valdir Sabbaga Amato, José Ângelo Lauletta Lindoso, Lucia Maria Almeida Braz

“…PB from suspected VL patients was evaluated by parasitological and molecular techniques using as the gold standard (GS) a combination of clinical, epidemiological, and immunochromatographic test (PB-rK39) results and parasitological examination of BM. …”
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An electrolyte-gated transistor for the monitoring of a CRISPR/Cas activity by Pierre Guermonprez, Le Tu Anh, Louis Renaud, Pierre Nioche, Eric Krejci, Aurélie Alleaume-Butaux, Nicolas Battaglini, Vu Thi Thu, Sébastien Sanaur, Benoît Piro

“…Reverse transcriptase-polymerase chain reaction (RT-PCR) remains the gold standard, but clustered regularly interspaced short palindromic repeats linked to a Cas endoribonuclease particle (CRISPR/Cas) have recently revolutionized the recognition step of two RNAs, i.e. the CRISPR-RNA (crRNA) and the target, providing a much better selectivity compared to the naked hybridization on which RT-PCR is based. …”
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Clinically relevant orthotopic pancreatic cancer models for adoptive T cell transfer therapy by Gregory B. Lesinski, Chrystal M. Paulos, Maggie Phillips, Jacklyn Hammons, Isaac Karpovsky, Zaid Mahdi, Natalie K. Horvat, Megan M. Wyatt, Margaret A. Hall, Cameron J. Herting, Richard A. Moffitt

“…Currently, genetically engineered mouse models (GEMMs) for PDAC are considered a “gold-standard” as they recapitulate many aspects of human disease. …”
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Integrated Evaluation of Age-Related Changes in Structural and Functional Vascular Parameters Used to Assess Arterial Aging, Subclinical Atherosclerosis, and Cardiovascular Risk in... by Daniel Bia, Yanina Zócalo, Ignacio Farro, Juan Torrado, Federico Farro, Lucía Florio, Alicia Olascoaga, Javier Brum, Walter Alallón, Carlos Negreira, Ricardo Lluberas, Ricardo L. Armentano

“…Subjects (n=388) were submitted to non-invasive vascular studies (gold-standard techniques), to evaluate (1) common (CCA), internal, and external carotid plaque prevalence, (2) CCA intima-media thickness and diameter, (3) CCA stiffness (percentual pulsatility, compliance, distensibility, and stiffness index), (4) aortic stiffness (carotid-femoral pulse wave velocity), and (5) peripheral and central pressure wave-derived parameters. …”
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Artificial intelligence-assisted platform performs high detection ability of hepatocellular carcinoma in CT images: an external clinical validation study by Rongxue Shan, Chenhao Pei, Qianrui Fan, Junchuan Liu, Dawei Wang, Shifeng Yang, Ximing Wang

“…The CT images were annotated and confirmed by the experienced radiologists using InferScholar software as the “gold standard”. The automatic HCC segmentation performed by the AI-assisted platform was used to compare with the annotation of radiologists. …”
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Prevalence, incidence and determinants of QuantiFERONTM positivity in South African schoolchildren by J. Stewart, N. Walker, K. Jennings, C. Delport, J Nuttall, A.K. Coussens, R Dyers, D.A. Jolliffe, J.C.Y. Tang, W.D. Fraser, R.J. Wilkinson, L.-G. Bekker, A.R. Martineau, K. Middelkoop

“…Prevalent infection was defined as QuantiFERON™-TB Gold Plus (QFT-Plus) positivity as assessed at baseline. …”
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