The beneficial effects of thymoquinone on viability and differentiation of human adipose tissue-derived mesenchymal stem cells by Ahsen Erginsoy, Nur Seda Gokdemir, Venhar Cinar, Eda Koseoglu, Zeynep Burcin Gonen, Tuba Dilay Kokenek Unal

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Identification of two new genetic loci for high-resolution genotyping of Enterocytozoon bieneusi by Meng Xinan, Ou Yonglin, Jiang Wen, Guo Yaqiong, Xiao Lihua, Feng Yaoyu, Li Na

“…In this study, comparison of a clinical genome sequence (C44566) with the whole genome sequence of an E. bieneusi isolate (H348) in GenBank led to the selection of the hypothetical protein 1 (hp1) and tubulin 1 (tub1) loci. …”
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SNP discovery by exome capture and resequencing in a pea genetic resource collection by Aubert, Grégoire, Kreplak, Jonathan, Leveugle, Magalie, Duborjal, Hervé, Klein, Anthony, Boucherot, Karen, Vieille, Emilie, Chabert-Martinello, Marianne, Cruaud, Corinne, Bourion, Virginie, Lejeune-Hénaut, Isabelle, Pilet-Nayel, Marie-Laure, Bouchenak-Khelladi, Yanis, Francillonne, Nicolas, Tayeh, Nadim, Pichon, Jean-Philippe, Rivière, Nathalie, Burstin, Judith

“…The development of this resource paves the way for Genome-wide association studies and the development of powerful genotyping tools. …”
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Linking obesity-associated genotype to child language development: the role of early-life neurology-related proteomics and brain myelinationResearch in context by Jian Huang, Jinyi Che, Michelle Z.L. Kee, Ai Peng Tan, Evelyn C. Law, Patricia Pelufo Silveira, Irina Pokhvisneva, Sachin Patel, Keith M. Godfrey, Lourdes Mary Daniel, Kok Hian Tan, Yap Seng Chong, Shiao-Yng Chan, Johan G. Eriksson, Dennis Wang, Jonathan Yinhao Huang

“…Interpretation: This study demonstrates the potential of examining early-life proteomics in conjunction with deep genotyping and phenotyping and provides biological insights into the shared genomic links between obesity and language development. …”
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Functional analysis of a novel homozygous missense IVD gene variant: a case report with dual genetic diagnoses by Yuying Zhu, Ke Wu, Hanying Wen

“…BackgroundGenomic or exome sequencing is beneficial for identifying more than one pathogenic variation causing blended atypical and/or severe phenotypes. …”
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Cryptic species and hybridisation in corals: challenges and opportunities for conservation and restoration by Riginos, Cynthia, Popovic, Iva, Meziere, Zoe, Garcia, Vhon, Byrne, Ilha, Howitt, Samantha M., Ishida, Hisatake, Bairos-Novak, Kevin, Humanes, Adriana, Scharfenstein, Hugo, Richards, Thomas, Briggs, Ethan, Clark, Vanessa, Lei, Chuan, Khan, Mariam, Prata, Katharine E.

“…Here, we investigate the prevalence of cryptic coral groups and assess evidence for their permeability to gene flow (hybridisation) via a structured literature review of genomic studies. Using reproducible criteria to detect distinct genetic groups that are sympatric, we find that 68% of nominal species represented in population genomic studies show evidence for comprising partially reproductively isolated groups and that these distinct groups are often linked by gene flow. …”
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Co-obligate symbioses have repeatedly evolved across aphids, but partner identity and nutritional contributions vary across lineages by Manzano-Marín, Alejandro, Coeur d’acier, Armelle, Clamens, Anne-Laure, Cruaud, Corinne, Barbe, Valérie, Jousselin, Emmanuelle

“…Lastly, patterns of Buchnera genome evolution reveal that small losses affecting a few key genes can be the onset of these dual systems, while large gene losses can occur without any co-obligate symbiont acquisition. …”
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Correlation among blastocoel fluid DNA level, apoptotic genes expression and preimplantation aneuploidy by Fattaneh Khajehoseini, Zahra Noormohammadi, Poopak Eftekhari-Yazdi, Hamid Gourabi, Reza Pazhoomand, Shirzad Hosseinishenatal, Masood Bazrgar

“…Following BF aspiration and whole genome amplification, BF-DNA level was quantified. …”
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T7 DNA polymerase treatment improves quantitative sequencing of both double-stranded and single-stranded DNA viruses by Billaud, Maud, Theodorou, Ilias, Lamy-Besnier, Quentin, Shah, Shiraz A., Lecointe, François, De Sordi, Luisa, De Paepe, Marianne, Petit, Marie-Agnès

“…Bulk microbiome, as well as virome-enriched shotgun sequencing only reveals the double-stranded DNA (dsDNA) content of a given sample, unless specific treatments are applied. However, genomes of viruses often consist of a circular single-stranded DNA (ssDNA) molecule. …”
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A catechol-O-methyltransferase genetic variant impacts functional movement in tactical athletes. by Marcus K Taylor, Lisa M Hernández, Richard C Allsopp, John J Fraser

“…In resource constrained settings, genomic modeling may help to direct limited assets to at-risk subgroups (for screening purposes). …”
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Chromatin remodeling (SWI/SNF) complexes, cancer, and response to immunotherapy by Razelle Kurzrock, Shumei Kato, Nithya Krishnamurthy, Scott Lippman

“…Significantly, ~20% of malignancies harbor alterations in >1 of these subunits, making the genes encoding SWI/SNF family members among the most vulnerable to genomic aberrations in cancer. ARID1A is the largest subunit of the SWI/SNF complex and is altered in ~40%–50% of ovarian clear cell cancers and ~15%–30% of cholangiocarcinomas, in addition to a variety of other malignancies. …”
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Author Correction: AIVariant: a deep learning-based somatic variant detector for highly contaminated tumor samples by Hyeonseong Jeon, Junhak Ahn, Byunggook Na, Soona Hong, Lee Sael, Sun Kim, Sungroh Yoon, Daehyun Baek

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Single-nucleotide polymorphisms in dizygotic twin ovine fetuses are associated with discordant responses to antenatal steroid therapy by Erin L. Fee, Haruo Usuda, Sean W. D. Carter, Hideyuki Ikeda, Tsukasa Takahashi, Yuki Takahashi, Yusaku Kumagai, Michael W. Clarke, Demelza J. Ireland, John P. Newnham, Masatoshi Saito, Sebastian E. Illanes, Binny Priya Sesurajan, Liang Shen, Mahesh A. Choolani, Gokce Oguz, Adaikalavan Ramasamy, Sara Ritchie, Andrew Ritchie, Alan H. Jobe, Matthew W. Kemp

“…We aimed to test the degree of concordance in preterm lung function, and correlate this with genomic, transcriptomic, and pharmacokinetic variables in preterm dizygotic twin ovine fetuses. …”
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Low & Anaplastic Grade Glioma Umbrella Study of MOlecular Guided TherapieS (LUMOS-2): study protocol for a phase 2, prospective, multicentre, open-label, multiarm, biomarker-direct... by Marshall Pitz, Eng-Siew Koh, Elizabeth H Barnes, Lucille Sebastian, Mandy L Ballinger, David M Thomas, Christopher O'Callaghan, John Simes, Hui K Gan, Sonia Yip, Hao-Wen Sim, Desma Spyridopoulos, Richard De Abreu Lourenco, Cynthia Hawkins, Kristen McParland, Benjamin Kong, Subotheni Thavaneswaran, Patrick J Wheeler

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Solid Predominant Histology and High Podoplanin Expression in Cancer-Associated Fibroblast Predict Primary Resistance to Osimertinib in EGFR-Mutated Lung Adenocarcinoma by Yuji Uehara, MD, Hiroki Izumi, MD, PhD, Tetsuro Taki, MD, PhD, Tetsuya Sakai, MD, PhD, Hibiki Udagawa, MD, PhD, Eri Sugiyama, MD, PhD, Shigeki Umemura, MD, PhD, Yoshitaka Zenke, MD, PhD, Shingo Matsumoto, MD, PhD, Kiyotaka Yoh, MD, PhD, Shoko Kubota, MD, PhD, Keiju Aokage, MD, PhD, Naoya Sakamoto, MD, PhD, Shingo Sakashita, MD, PhD, Motohiro Kojima, MD, PhD, Michiko Nagamine, MD, PhD, Yukio Hosomi, MD, PhD, Masahiro Tsuboi, MD, PhD, Koichi Goto, MD, PhD, Genichiro Ishii, MD, PhD

“…We also investigated HGF mRNA expression levels, using The Cancer Genome Atlas Program and Singapore Oncology Data Portal cohorts. …”
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Polygenic prediction of human longevity on the supposition of pervasive pleiotropy by M. Reza Jabalameli, Jhih-Rong Lin, Quanwei Zhang, Zhen Wang, Joydeep Mitra, Nha Nguyen, Tina Gao, Mark Khusidman, Sanish Sathyan, Gil Atzmon, Sofiya Milman, Jan Vijg, Nir Barzilai, Zhengdong D. Zhang

“…We showed that coefficients derived from our ensemble model could potentially reveal an interesting pattern of genomic pleiotropy specific to lifespan. We assessed the predictive performance of our model for distinguishing the enrichment of exceptional longevity among long-lived individuals in two replication cohorts (the Scripps Wellderly cohort and the Medical Genome Reference Bank (MRGB)) and showed that the median lifespan in the highest decile of our composite prognostic index is up to 4.8 years longer. …”
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Optimized gene transduction in human lung organoids: A high-efficiency method for advanced research applications by Jasmin Khateeb, Jady Liang, Yuchong Li, Thenuka Thanabalasingam, Julie Khang, Mirjana Jerkic, Giovanna Pellecchia, Bhooma Thiruv, Ya-Wen Chen, Ori Rotstein, Arthur S. Slutsky, Haibo Zhang

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Genetic Evidence for Geographic Structure within the  Neanderthal Population by Rogers, Alan R.

“…It argues that the observed pattern results from subdivision and gene flow. If two haploid genomes are sampled from the same subpopulation, their recent ancestors are likely to be geographic neighbors and therefore coalesce rapidly. …”
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Egg Microinjection for the Ladybird Beetle Harmonia axyridis by Taro Nakamura, Yuji Matsuoka, Teruyuki Niimi

“…Our step-by-step protocol is applicable to generating transgenic or genome-edited ladybird beetles, facilitating functional genetics in H. axyridis; the microinjection method should be applicable to other insect eggs.…”
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Generation of an induced pluripotent stem cell line (UCLi026-A) from a patient with ADCY5-related disease carrying the heterozygous variant c.1253G > A; (p. Arg418Gln) by Sharmin Alhaque, Dimitri Budinger, Barbara Garavaglia, Giovanna Zorzi, Serena Barral, Manju A. Kurian

“…This line was further characterised for pluripotency, differentiation potential and genomic integrity. This cell line, UCLi026-A (UCL-NG-ADCY5-001) can be utilized for in vitro disease modelling of ADCY5-related diseases, as well as for the development of novel therapeutic approaches.…”
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