Development of <i>Aspergillus oryzae</i> BCC7051 as a Robust Cell Factory Towards the Transcriptional Regulation of Protease-Encoding Genes for Industrial Applications by Sarocha Panchanawaporn, Chanikul Chutrakul, Sukanya Jeennor, Jutamas Anantayanon, Kobkul Laoteng

“…Here, we improved food-grade <i>Aspergillus oryzae</i> BCC7051 by manipulating the transcriptional regulation of protease-encoding genes. Genome mining of the transcription factor <i>AoprtR</i> and computational analysis confirmed its deduced amino acid sequence sharing evolutionary conservation across <i>Aspergillus</i> and <i>Penicillium</i> spp. …”
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Phylogenetic analysis of dermatophytes isolated from small domestic animals by A. M. Smagulova, E. V. Kukhar, T. I. Glotova, A. G. Glotov

“…The comparative analysis of the genomes of the Microsporum and Trichophyton fungi and reference strains revealed a relatively low level of intraspecies polymor- phism and point mutations of the sequences. …”
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Polymorphisms in Adipokines in Mexican Children with Obesity by Angélica Saraí Jiménez-Osorio, Alma Olivia Aguilar-Lucio, Helios Cárdenas-Hernández, Claudette Musalem-Younes, Jacqueline Solares-Tlapechco, Paula Costa-Urrutia, Oscar Medina-Contreras, Julio Granados, Martha Eunice Rodríguez-Arellano

“…We propose to investigate the contribution of adipokines and cytokines polymorphisms and common BMI/obesity-associated loci, revealed in genome-wide association studies in Caucasian adult cohorts, with childhood obesity. …”
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Presence of the Anopheles culicifacies complex species A in southeast Iran by Seyed Massoud Madjdzadeh, Nahid Behi, Mohammad Amin Gorouhi, Arsalan Amirkafi, Mohammad Ali Oshaghi

“…DNA was extracted from mosquito specimens, and the D3 region of the 28S rDNA gene and a segment of the COII gene from the mitochondrial genome (mtDNA) were targeted for amplification and sequencing. …”
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Integrating phylogeographic and phenotypic evidence to delimit deep evolutionary lineages in the Dryophytes japonicus species complex, with an assessment of their conservation need... by Amaël Borzée, Yikweon Jang, Siti N. Othman, Jordy Groffen, Irina V. Maslova, Zoljargal Purevdorj, Kiyomi Yasumiba, Tomohiko Shimada, Yoonjung Yi, Natalya Alekseevna Schepina, Desiree Andersen, Yoonhyuk Bae, Ikuo Miura, Christophe Dufresnes

“…In this study, we assessed the genetic, genomic, morphological, and acoustic diversity and differentiation within the D. japonicus complex to propose taxonomic arrangements and assess taxon-specific threats for each defined clade. …”
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The case for development of a core outcome set (COS) and supplemental reporting guidelines for influenza vaccine challenge trial research in swine by Sheila Keay, Famke Alberts, Annette M. O’Connor, Robert Friendship, Terri O’Sullivan, Zvonimir Poljak

“…The checklist serves two purposes: as a reminder of details essential to report for inclusion of findings in meta-analyses and sub-group meta-analyses (e.g., antigenic or genomic descriptions of influenza vaccine and challenge viruses), and as an aid to help synthesis researchers fully characterize and comprehensively include studies in reviews.…”
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Resistance-Associated Substitutions (RAS) and Clinical Factors as Determinants of Sofosbuvir-Daclatasvir Treatment Outcomes in Chronic Hepatitis C Patients by Juferdy Kurniawan, Anugrah Dwi Handayu, Gita Aprilicia, Darlene Raudhatul Bahri, Irsan Hasan

“…One particular concern associated with treatment failure is the emergence of resistance associated substitutions (RASs) in the genome. The occurrence of RASs highlights the adaptability and resilience of the HCV. …”
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Using Adipose Measures from Health Care Provider-Based Imaging Data for Discovery by Elliot D. K. Cha, Yogasudha Veturi, Chirag Agarwal, Aalpen Patel, Mohammad R. Arbabshirani, Sarah A. Pendergrass

“…Sex-combined and sex-stratified association testing was done between adipose measures and 1,233 disease diagnoses and 37 clinical laboratory measures. A genome-wide association study (GWAS) for adipose measures was also performed. …”
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Use of a synthetic form Avrodes for transfer of leaf rust resistance from Aegilops speltoides to common wheat by R. O. Davoyan, I. V. Bebyakina, E. R. Davoyan, D. S. Mikov, E. D. Badaeva, I. G. Adonina, E. A. Salina, A. N. Zinchenco, Y. S. Zubanova

“…Hence it was assumed that new genes were introduced into the common wheat genome from Ae. speltoides. Introgression lines have been studied for productivity and technological qualities of grain. …”
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Promoter Methylation Precedes Chromosomal Alterations in Colorectal Cancer Development by Sarah Derks, Cindy Postma, Peter T. M. Moerkerk, Sandra M. van den Bosch, Beatriz Carvalho, Mario A. J. A. Hermsen, Walter Giaretti, James G. Herman, Matty P. Weijenberg, Adriaan P. de Bruïne, Gerrit A. Meijer, Manon van Engeland

“…Chromosomal alterations were evaluated by comparative genomic hybridization. Results: Our data demonstrate that nonprogressed adenomas, progressed adenomas and carcinomas show similar frequencies of promoter methylation for the majority of the genes. …”
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Sulfatase modifying factor 2 as a predictive biomarker for urothelial carcinoma by Wei-Ting Kuo, Yi-Chen Lee, Jia-Bin Liao, Ching-Jiunn Tseng, Yi-Fang Yang

“…High SUMF2 mRNA levels were associated with poor overall survival (OS) and disease-free survival (DFS) in bladder UC (BLCA) from The Cancer Genome Atlas (TCGA) dataset. High SUMF2 protein levels were associated with grade (P < 0.001), T status (P = 0.01), and stage (P = 0.006) in patients with BLCA. …”
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Genetic polymorphism of modern common flax (Linum usitatissimum L.) cultivars developed at Russian breeding centers using SSR markers by T. A. Bazanov, I. V. Ushchapovskii, V. A. Lemesh, M. V. Bahdanava, A. V. Lahunovskaya

“…Molecular identification of plant genomes, based on the use of DNA markers, is becoming a reliable tool for cultivar identification. …”
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Multi-tissue network analysis reveals the effect of JNK inhibition on dietary sucrose-induced metabolic dysfunction in rats by Hong Yang, Cheng Zhang, Woonghee Kim, Mengnan Shi, Metin Kiliclioglu, Cemil Bayram, Ismail Bolar, Özlem Özdemir Tozlu, Cem Baba, Nursena Yuksel, Serkan Yildirim, Shazia Iqbal, Jihad Sebhaoui, Ahmet Hacımuftuoglu, Matthias Uhlen, Jan Boren, Hasan Turkez, Adil Mardinoglu

“…Coexpression analysis and genome-scale metabolic modeling reveal that sucrose overconsumption primarily induces transcriptional dysfunction related to fatty acid and oxidative metabolism in the liver and adipose tissues, which are largely rectified after JNK inhibition at a clinically relevant dose. …”
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Identification of a new alphacoronavirus (Coronaviridae: <i>Alphacoronavirus</i>) associated with the greater horseshoe bat (<i>Rhinolophus ferrumequinum</i>) in the south of Europ... by Sergey V. Lenshin, Tatyana V. Vishnevskaya, Alexey V. Romashin, Yulia I. Bulycheva, Oleg I. Vyshemirsky, Sophya A. Solovyeva, Asya K. Gitelman, Alexey S. Pazilin, Dmitry K. Lvov, Ben Hu, Zheng-Li Shi, Sergey V. Alkhovsky

“…Fecal samples were collected from bats inhabiting caves on the southern macroslope of the Greater Caucasus (Sochi-Adler region) during 2020, 2021, and 2024. Viral genomes were detected and analyzed using high-throughput sequencing (NGS) and RT-PCR. …”
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Development of polymerase chain reaction kit for detection of SARS-CoV-2 RNA in biological samples collected from animals by A. M. Timina, A. S. Yakovleva, M. V. Timanov, M. V. Biryuchenkova, Ye. S. Orlova

“…Animal populations in different regions of the Russian Federation were subjected to the screening tests in order to detect the novel coronavirus genome. NoSARS-CoV-2 was reported in her bivorous animals in the Russian Federation. …”
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Identified VCAM1 as prognostic gene in gastric cancer by co-expression network analysis by Wenjuan Li, Hong Gao, Jianjun Liu

“…Data from RNA sequencing and clinical pathological details were acquired from The Cancer Genome Atlas (TCGA) database and the Gene Expression Omnibus (GEO) dataset. …”
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Programmed cell death-related gene IL20RA facilitates tumor progression and remodels tumor microenvironment in thyroid cancer by Yuqi Wang, Yunlong Zhang, Min Liu, Yu Liu, Yu Zeng, Wei Zhang, Shuping Wu, Linfei Hu, Xianhui Ruan, Xiangqian Zheng, Ming Gao, Jingzhu Zhao

“…Data from The Cancer Genome Atlas (TCGA) database was utilized to develop a PCD-related risk prediction model based on LASSO regression along with univariate Cox regression. …”
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Insufficient gene expression and lost gene regulatory network may underlie the mechanism of Hirschsprung Disease in 5p–syndrome by Yizhao Luan, Peng Li, Yuanyuan Luo, Hong Zhang, Xiaochun Zhu, Yan Zhang, Aihua Yin, Qiang Wu, Chengwei Chai

“…Here, we conducted a study on two patients with CDC and HSCR using whole genome sequencing (WGS) analyses. Our WGS data confirmed the deletion regions on 5p associated with CDC and indicated potential unknown genetic mechanisms underlying HSCR. …”
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Actively protective combinatorial analysis: A scalable novel method for detecting variants that contribute to reduced disease prevalence in high-risk individuals by J Sardell, S Das, K Taylor, C Stubberfield, A Malinowski, M Strivens, S Gardner

“…We show how this can be used to identify mechanisms in the background of normal cellular biology that work to slow or stop progression of complex, chronic diseases.Actively protective combinatorial analysis identifies combinations of features that contribute to reducing risk of disease in individuals who remain healthy even though their genomic profile suggests that they have high risk of developing disease. …”
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Single-cell sequencing uncovers the mechanistic role of DAPK1 in glioma and its diagnostic and prognostic implications by Tian-Hang Yu, Yan-Yu Ding, Yan-Yu Ding, Si-Guo Zhao, Jie-Hui Zhao, Yu Gu, Dong-Hui Chen, Fang Zhang, Wen-Ming Hong, Wen-Ming Hong

“…However, the precise role and underlying mechanisms of DAPK1 in gliomas remain inadequately understood.MethodsWe performed analyses on RNA-seq and microarray datasets from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), in addition to single-cell RNA sequencing (scRNA-seq) data from glioma patients available in GEO. …”
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