Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC by Yulin Zhang, Yixu Wang, Jilin Peng, Kun Zhao, Ling Li, Yuan Zhang, Ziyu Zhai, Sijie Yuan, Shichao Li, Shichao Li, Shichao Li, Fanglei Ye, Le Wang

“…BackgroundN6-methyladenosine (m6A) RNA modification is crucial for tumor development and progression; however, which m6A regulators play a pivotal role in head and neck squamous cell carcinoma (HNSCC) remains ambiguous.MethodsUtilizing the Cancer Genome Atlas (TCGA) database, the expression levels of m6A regulators in HNSCC were examined, which led to the identification of heterogeneous nuclear ribonucleoprotein C (HNRNPC) as a key gene. …”
Get full text

Extreme Environment Streptomyces: Potential Sources for New Antibacterial and Anticancer Drug Leads? by Periyasamy Sivalingam, Kui Hong, John Pote, Kandasamy Prabakar

“…It is because of technical advances in isolation, fermentation, spectroscopy, and genomic studies which led to the efficient recovering of Streptomyces and their new chemical compounds with distinct activities. …”
Get full text

The Studies of Chlorogenic Acid Antitumor Mechanism by Gene Chip Detection: The Immune Pathway Gene Expression by Tian Yi Kang, Hua Rong Yang, Jie Zhang, Dan Li, Jie Lin, Li Wang, XiaoPing Xu

“…Here, we have utilized a whole mouse genome oligo microarray (4*44K) to analyze gene expression level of female BALB/c mice (implanted with EMT-6 sarcoma cells) after treatment with low, medium, and high-dose CA (5 mg/kg, 10 mg/kg, and 20 mg/kg), docetaxel, interferon, and normal saline separately at 6 time points (3rd, 6th, 9th, 12th, 15th, and 18th days after administration). …”
Get full text

Bioprospecting of a Native Plant Growth-Promoting Bacterium <i>Bacillus cereus</i> B6 for Enhancing Uranium Accumulation by Sudan Grass (<i>Sorghum sudanense</i> (Piper) Stapf) by Longyuan Wu, Lijuan Zhang, Ning Wang, Wei Huang, Yanzhi Wang, Meng Sun, Guofeng Zheng, Wei Wang, Chong Shi

“…The bacterium was identified as Bacillus cereus. Genomic analyses and assessment of uranium tolerance-promoting properties showed that strain B6 not only exhibited high uranium tolerance, but also possessed beneficial properties such as phosphorus solubilization and iron-producing carriers. …”
Get full text

An assessment of circulating biomarkers and germline genetic variants in predicting chemotherapy associated mucositis in Ewing sarcoma by Pawan Gulati, Vickyanne Carruthers, Claire Hutton, Chloe Cassidy, Edward B. Amankwatia, Séréna Pascual, Electra Florence, Tsegay G. Gebru, Andrea L. Jorgensen, Alastair Greystoke, Guy Makin, Martin G. McCabe, Daniel B. Hawcutt, Gareth J. Veal, David Jamieson

“…Discussion: Early increases in circulating CK18 levels correlated with grade 3 mucositis. Genome-wide association analysis highlighted genes that may be associated with mucositis pathology, offering insights into biological mechanisms underlying susceptibility.…”
Get full text

Rapid detection of FAdV-4 by one-tube RPA-CRISPR/Cas12a assay by Lei Ma, Xueping Wang, Mingliang Zhang, Mengjie Zhu

“…This approach reduced contamination.ResultsThe RPA-CRISPR/Cas12a detection method can identify as few as 10 copies of the genome per reaction, demonstrating 100% sensitivity comparable to that of fluorescence PCR (qPCR). …”
Get full text

Transgenic plants as a model for studying еpigenetic regulation of gene expression by T. V. Marenkova, E. V. Deineko

“…The study of this phenomenon revealed dependence of the frequency of gene silencing on the number of integrated copies in the plant genome, the properties of the transgene sequence itself (the presence of duplications, vector sequences, and others), chromosomal position. …”
Get full text

Characterization of Microbiota in Endometrial Fluid and Vaginal Secretions in Infertile Women with Repeated Implantation Failure by Kotaro Kitaya, Yoko Nagai, Wataru Arai, Yoshiyuki Sakuraba, Tomomoto Ishikawa

“…On days 6-8 in the luteal phase of the natural, oocyte-pickup, or hormone replacement cycle, the paired EF and VS samples were obtained separately. Extracted genomic DNA was pyrosequenced for the V4 region of 16S ribosomal RNA using a next-generation sequencer. …”
Get full text

Epigenetic Alterations and an Increased Frequency of Micronuclei in Women with Fibromyalgia by Victoria Menzies, Debra E. Lyon, Kellie J. Archer, Qing Zhou, Jenni Brumelle, Kimberly H. Jones, G. Gao, Timothy P. York, Colleen Jackson-Cook

“…To determine if acquired epigenetic/genetic changes are associated with FM, we compared the frequency of spontaneously occurring micronuclei (MN) and genome-wide methylation patterns in women with FM (n=10) to those seen in comparably aged healthy controls (n=42 (MN); n=8 (methylation)). …”
Get full text

Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage by Ying Zheng, Xi Luo, Zailong Qin, Zhiguang Zhou

“…Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses were performed to predict the functions of dysregulated genes. …”
Get full text

Transcriptomic-Based Identification of miR-125a Novel Targets in Human Hepatocarcinoma Cells by Ilenia De Leo, Nicola Mosca, Mariaceleste Pezzullo, Danila Valletta, Francesco Manfrevola, Vincenza Grazia Mele, Rosanna Chianese, Aniello Russo, Nicoletta Potenza

“…However, its HCC relevant targets and molecular mechanisms are still largely unknown. Here, a genome-wide perspective of the whole miR-125a targetome has been achieved. …”
Get full text

Establishment and characterization of ovarian clear cell carcinoma patient-derived xenografts by Joseph J. Caumanns, Shang Li, Gert J. Meersma, Evelien W. Duiker, Ate G. J. van der Zee, G. Bea A. Wisman, Steven de Jong

“…SNP array and Infinium MethylationEPIC BeadChip array data analysis demonstrated the copy number alterations and DNA methylation signatures of genome-wide and tumor-driver genes in PDXs generally resembled their patients’ tumors. …”
Get full text

OMIC Technologies and Vaccine Development: From the Identification of Vulnerable Individuals to the Formulation of Invulnerable Vaccines by Nicola Cotugno, Alessandra Ruggiero, Veronica Santilli, Emma Concetta Manno, Salvatore Rocca, Sonia Zicari, Donato Amodio, Manuela Colucci, Paolo Rossi, Ofer Levy, Federico Martinon-Torres, Andrew J. Pollard, Paolo Palma

“…This review describes innovative and experimental approaches that can help identify specific genomic profiles defining nonresponder individuals for whom specific interventions might be needed. …”
Get full text

Characterization and functional analysis of chicken hnRNP protein by Ke Wang, Manzi Huang, Qinghua Zeng, Huansheng Wu

“…In this study, multiple chicken hnRNPs were identified to interact with genomic dsRNA. Initially, these hnRNPs were found to be exclusively localized in the nucleus before IBDV infection. …”
Get full text

SCO6564, a novel 3-ketoacyl acyl carrier protein synthase III, contributes in fatty acid synthesis in Streptomyces coelicolor. by Jian-Rong Ma, Jia-Ying Lin, Yuan-Yin Zhang, Yun Chen, Wen-Bing Zhang, Xian-Pu Ni, Yong-Hong Yu

“…In this study, the FabH homolog SCO6564 (named FabH2) was identified in the S. coelicolor genome by BLAST analysis. The expression of fabH2 restored the growth of Ralstonia solanacearum fabH mutant and made the mutant produce small amounts of branched-chain fatty acids. …”
Get full text

Molecular complexity of quantitative immunity in plants: from QTL mapping to functional and systems biology by Chauveau, Carine, Roby, Dominique

“…First, from genetic analyses by QTL (Quantitative Trait Locus) mapping and GWAs (Genome Wide Association studies), the identification, cloning and functional analysis of this gene have been used as a starting point for the exploration of the multiple and coordinated pathways acting together to mount the QDR response dependent on RKS1. …”
Get full text

Formation mechanisms of ethyl acetate and organic acids in Kluyveromyces marxianus L1-1 in Chinese acid rice soup by Na Liu, Likang Qin, Laili Hu, Song Miao

“…At least 4231 genes and 2937 proteins were identified and 610 differentially expressed proteins were annotated to 30 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways based on the analysis results of transcriptome and proteome. …”
Get full text

Engineering a phi15-based expression system for stringent gene expression in Pseudomonas putida by Eveline-Marie Lammens, Daniel C. Volke, Alison Kerremans, Yannick Aerts, Maarten Boon, Pablo I. Nikel, Rob Lavigne

“…The system functions well in combination with several inducer systems, and in a variety of vector-based and genomically integrated set-ups. In conclusion, the phi15 RNAP, promoter, lysozyme and growth-decoupler provide a valuable plug-and-play set of genetic parts for the P. putida toolbox.…”
Get full text

NERVE 2.0: boosting the new enhanced reverse vaccinology environment via artificial intelligence and a user-friendly web interface by Andrea Conte, Nicola Gulmini, Francesco Costa, Matteo Cartura, Felix Bröhl, Francesco Patanè, Francesco Filippini

“…Abstract Background Vaccines development in this millennium started by the milestone work on Neisseria meningitidis B, reporting the invention of Reverse Vaccinology (RV), which allows to identify vaccine candidates (VCs) by screening bacterial pathogens genome or proteome through computational analyses. …”
Get full text

The Role of Mitochondrial NADPH-Dependent Isocitrate Dehydrogenase in Cancer Cells by Katarína Smolková, Petr Ježek

“…Arg172 mutants of human IDH2—frequently found with similar mutants of cytosolic IDH1 in grade 2 and 3 gliomas, secondary glioblastomas, and acute myeloid leukemia—catalyze reductive carboxylation of 2-oxoglutarate and reduction to D-2-hydroxyglutarate, which strengthens the neoplastic phenotype by competitive inhibition of histone demethylation and 5-methylcytosine hydroxylation, leading to genome-wide histone and DNA methylation alternations. …”
Get full text