Association between Polymorphisms in Interleukin-17A and -17F Genes and Chronic Periodontal Disease by Jôice Dias Corrêa, Mila Fernandes Moreira Madeira, Renata Gonçalves Resende, Jeane de Fátima Correia-Silva, Ricardo Santiago Gomez, Danielle da Glória de Souza, Mauro Martins Teixeira, Celso Martins Queiroz-Junior, Tarcília Aparecida da Silva

“…Material and Methods. Genomic DNA was obtained from 30 CP patients and 30 healthy controls (HCs). …”
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Screening and Functional Verification of Selectable Marker Genes for Cordyceps militaris by Haiwei Lou, Yu Zhao, Renyong Zhao, Zhiwei Ye, Junfang Lin, Liqiong Guo

“…The bar gene of the vector pCAMBIA0390-Bar was successfully integrated into the C. militaris genome and could be highly expressed in the transformants of C. militaris. …”
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AutoPVPrimer: A comprehensive AI-Enhanced pipeline for efficient plant virus primer design and assessment. by Abozar Ghorbani, Mahsa Rostami, Elham Ashrafi-Dehkordi, Pietro Hiram Guzzi

“…The pipeline uses Biopython to automatically retrieve different genomic sequences from the NCBI database to increase the robustness of the subsequent primer design. …”
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Transmitted Drug Resistance among People Living with HIV/Aids at Major Cities of Sao Paulo State, Brazil by Joao Leandro Paula Ferreira, Rosangela Rodrigues, Andre Minhoto Lança, Valeria Correia de Almeida, Simone Queiroz Rocha, Taisa Grotta Ragazzo, Denise Lotufo Estevam, Luis Fernando de Macedo Brigido

“…Seventy-six percent of genomes (13/17) with TDR carried a nonnucleoside reverse transcriptase inhibitor (NNRTI) resistance mutation, mostly K103N/S (9/13, 69%), potentially compromising the preferential first-line therapy suggested by the Brazilian HIV Treatment Guideline that recommends efavirenz-based combinations. …”
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Isolation and characterization of a highly pathogenic strain of Vibrio harveyi EFL-2201 from pearl gentian grouper by Biao Yuan, Zhixia Zhuang, Xiaoru Wang, Huabin Huang, Qingpi Yan

“…In vitro assays indicated that the conditions during the outbreak were conducive to the proliferation, motility, biofilm formation, and chemotaxis of EFL-2201. Whole-genome sequencing of the strain revealed that mechanisms related to adhesion, secretion, toxins, and invasion significantly contributed to its pathogenicity. …”
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Identification of Functional Modules and Key Pathways Associated with Innervation in Graft Bone—CGRP Regulates the Differentiation of Bone Marrow Mesenchymal Stem Cells via p38 MAP... by Ziqian Wu, Xudong Wang, Jingcun Shi, Anand Gupta, Yuhan Zhang, Bingqing Zhang, Yang Cao, Lei Wang

“…We identified differentially expressed genes) from these samples and used the gene ontology and Kyoto Encyclopedia of Genes and Genomes databases for functional enrichment and signaling pathway analysis. …”
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Epigenetic Properties of Compounds Contained in Functional Foods Against Cancer by Giulia Casari, Brenda Romaldi, Andrea Scirè, Cristina Minnelli, Daniela Marzioni, Gianna Ferretti, Tatiana Armeni

“…Epigenetics encompasses reversible and heritable genomic changes in histones, DNA expression, and non-coding RNAs that occur without modifying the nucleotide DNA sequence. …”
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DNAPred_Prot: Identification of DNA-Binding Proteins Using Composition- and Position-Based Features by Omar Barukab, Yaser Daanial Khan, Sher Afzal Khan, Kuo-Chen Chou

“…In the domain of genome annotation, the identification of DNA-binding protein is one of the crucial challenges. …”
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Causal role of ischemic heart disease in ovarian cancer subtypes by Jianhai Chen, Huabin He, Ling Gao

“…Methods A two-sample MR analysis was conducted using genetic instruments for IHD from large-scale genome-wide association studies (GWAS). The inverse-variance weighted (IVW) method was used as the primary analysis, supported by MR-Egger, weighted median, and MR-PRESSO for sensitivity analyses. …”
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Adeno-associated virus-based gene therapy for hemophilia–addressing the gaps by Wolfgang Miesbach, Paul Batty, Pratima Chowdary, Sylvia Fong, Radoslaw Kaczmarek, Frank W.G. Leebeek, Brian Long, Johnny Mahlangu, Mike Makris, Glenn F. Pierce, Steven W. Pipe, Alok Srivastava, Jan Voorberg, Flora Peyvandi

“…This manuscript follows literature reviews and structured discussions by the International Society on Thrombosis and Haemostasis Scientific and Standardization Committee Working Group on Gene Therapy that identified specific areas requiring standardization of methods, including viral vector production, liver function assessment, quantification of factor (F)VIII and FIX expression levels, assessment of antiadeno-associated viral antibodies, and genomic integration detection methods. Standardization strategies aim to achieve consistent vector quality, effective patient selection, and uniform assessment methods by implementing advanced laboratory techniques and standardized protocols. …”
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Differential pathological changes in colon microenvironments in acute and chronic mouse models of inflammatory bowel disease by NaYeon Ham, Minji Park, Young-An Bae, Eui-Ju Yeo, YunJae Jung

“…RNA-sequencing revealed genetic changes in acute and chronic colitis, marked by the maintenance of genomic integrity in the acute phase and extracellular matrix dynamics in the chronic phase. …”
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The Role of the Pathoanatomical Diagnosis «Chronic Gastritis» in the System of Personified Cancer Prevention by A. V. Kononov

“…The activity of gastritis (infiltration of the mucosa with neutrophilic leukocytes) of any etiology is the theoretical justification for the use of bismuth tripotassium dicitrate pharmacological preparation to protect the genome of stem cells of the gastric epithelium as a secondary prevention of gastric cancer.…”
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A TaqMan Real-Time PCR Assay for Detection and Quantification of Sporisorium scitamineum in Sugarcane by Yachun Su, Shanshan Wang, Jinlong Guo, Bantong Xue, Liping Xu, Youxiong Que

“…This method was more sensitive (a detection limit of 10 ag pbE DNA and 0.8 ng sugarcane genomic DNA) than that of conventional PCR (10 fg and 100 ng, resp.). …”
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Essential oils modulate virulence phenotypes in a multidrug-resistant pyomelanogenic Pseudomonas aeruginosa clinical isolate by Jayalekshmi Haripriyan, Christy Rose Binu, Nitasha D Menon, Muralidharan Vanuopadath, Malavika B. Hari, N Namitha, Kesiya Binoy, Anil Kumar, Bipin G. Nair, Victor Nizet, Geetha B. Kumar

“…Abstract Pyomelanogenic P. aeruginosa, frequently isolated from patients with urinary tract infections and cystic fibrosis, possesses the ability to withstand oxidative stress, contributing to virulence and resulting in persistent infections. Whole genome sequence analysis of U804, a pyomelanogenic, multidrug-resistant, clinical isolate, demonstrates the mechanism underlying pyomelanin overproduction. …”
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In vitro long-term exposure to chlorhexidine or triclosan induces cross-resistance against azoles in Nakaseomyces glabratus by Kathrin Spettel, Dominik Bumberger, Richard Kriz, Sarah Frank, Madita Loy, Sonia Galazka, Miranda Suchomel, Heimo Lagler, Athanasios Makristathis, Birgit Willinger

“…Susceptibility testing and whole genome sequencing of yeast isolates pre- and post-exposure were performed. …”
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Epigenetic Methylation of Parathyroid CaR and VDR Promoters in Experimental Secondary Hyperparathyroidism by Jacob Hofman-Bang, Eva Gravesen, Klaus Olgaard, Ewa Lewin

“…Melting temperature profiling of CaR and VDR PCR products after bisulfite treatment of genomic DNA from rat parathyroids was performed. Real-time PCR measured expression of PTH, CaR, VDR, and klotho genes in vitro. …”
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RNASeq analysis of drought-stressed guayule reveals the role of gene transcription for modulating rubber, resin, and carbohydrate synthesis by Chen Dong, Grisel Ponciano, Naxin Huo, Yong Gu, Daniel Ilut, Colleen McMahan

“…To better understand how drought influences rubber biosynthesis at the molecular level, a comprehensive transcriptome database was built from drought-stressed guayule stem tissues using de novo RNA-seq and genome-guided assembly, followed by annotation and expression analysis. …”
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Insights into the molecular mechanisms and nanoparticle-based therapies for gastric cancer: A review by Smarika Chauhan, Eswar Marcharla, Sneha Hariharan, Saranya Vinayagam, Thanigaivel Sundaram, Priya SD, Swamynathan Ganesan, Maximilian Lackner

“…This review aims to explore the role of nanoparticles in the prognosis and diagnosis of gastric cancer. We have analysed genomic pathways linked with gastric carcinoma and explored the utilisation of nanoparticles for multimodal imaging techniques, precise drug delivery and efficient therapeutic approaches. …”
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The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma by Shahid Banday, Alok K. Mishra, Romana Rashid, Tianyi Ye, Amjad Ali, Junhui Li, Jason T. Yustein, Michelle A. Kelliher, Lihua Julie Zhu, Sara K. Deibler, Sunil K. Malonia, Michael R. Green

“…Factors that promote EWSR1::FLI1 expression, and thus whose inhibition would reduce EWSR1::FLI1 protein levels and function, are potential drug targets. Here, using genome-scale CRISPR/Cas9 knockout screening, we identify C1GALT1, a galactosyltransferase required for the biosynthesis of many O-glycoproteins, as a factor that promotes EWSR1::FLI1 expression. …”
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Challenges in Emerging Vaccines and Future Promising Candidates against SARS-CoV-2 Variants by Tanmay Ghildiyal, Nishant Rai, Janhvi Mishra Rawat, Maargavi Singh, Jigisha Anand, Gaurav Pant, Gaurav Kumar, Amrullah Shidiki

“…The review provides an integrative discussion on the current state of knowledge on the type of vaccines developed against SARS-CoV-2, the safety and efficacy of COVID-19 vaccines concerning the VOCs, and prospects of novel universal, chimeric, and bivalent mRNA vaccines efficacy to fend off existing variants and other emerging coronaviruses. Genomic variation can be quite significant, as seen by the notable differences in impact, transmission rate, morbidity, and death during several human coronavirus outbreaks. …”
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