Heterologous Expression and Transglycosylation of Acarviosyltransferase by XUE Zhenglian, WANG Yuqing, LI Chuang, LI Dandan, ZHU Sibao, LI Xiangfei

“…In order to further investigate the structural properties and catalytic function of acarviosyltransferase (ATase), a key enzyme in the biosynthesis of acarbose, its gene (acbD) was cloned from Actinoplanes sp. SE50 genome and heterologously expressed in Escherichia coli. …”
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Proteogenomic analysis reveals Arp 2/3 complex as a common molecular mechanism in high risk pancreatic cysts and pancreatic cancer by A. K. M. Firoj Mahmud, Dina Gamaleldin Mansour Aly, Yelin Zhao, Mikael Benson, Martin Smelik, Oleg Sysoev, Hui Wang, Xinxiu Li

“…This study investigates the genetic architecture of benign pancreatic cysts and its potential connection to PC using genome-wide association studies (GWAS). The discovery GWAS identified significant genetic variants associated with benign cysts, specifically the rs142409042 variant near the OPCML gene. …”
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Relationship between Maternal Immunological Response during Pregnancy and Onset of Preeclampsia by Alicia Martínez-Varea, Begoña Pellicer, Alfredo Perales-Marín, Antonio Pellicer

“…The maternal immunological tolerance to the semiallogeneic fetus becomes greater in egg donation pregnancies with unrelated donors as the complete fetal genome is allogeneic to the mother. Instead of being rejected, the allogeneic fetus is tolerated by the pregnant woman in egg donation pregnancies. …”
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Activation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton by Brendan W. Mormile, Yan Yan, Taran Bauer, Li Wang, Rachel C. Rivero, Sara C. D. Carpenter, Catherine Danmaigona Clement, Kevin L. Cox, Lin Zhang, Xiyu Ma, Terry A. Wheeler, Jane K. Dever, Ping He, Adam J. Bogdanove, Libo Shan

“…To understand the pathogenicity and host adaptation of Xanthomonas citri pv. malvacearum (Xcm), we assemble the genome and the TALE repertoire of three recent Xcm Texas isolates. …”
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Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo by Yilai Shu, Yong Tao, Wenyan Li, Jun Shen, Zhengmin Wang, Zheng-Yi Chen

“…To identify adenovirus vectors that target specific cell subtypes in the inner ear, we studied three adenovirus vectors, carrying a reporter gene encoding green fluorescent protein (GFP) from two vendors or with a genome editing gene Cre recombinase (Cre), by injection into postnatal days 0 (P0) and 4 (P4) mouse cochlea through scala media by cochleostomy in vivo. …”
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Urinary Catheter Colonization by Multidrug-Resistant Cedecea neteri in Patient with Benign Prostatic Hyperplasia by Peter S. Ginn, Serina B. Tart, Stephen M. Sharkady, Dorothea K. Thompson

“…This C. neteri isolate was resistant or intermediate to second-generation cephalosporins, penicillins, and certain β-lactamase inhibitor/β-lactam combinations. Analysis of whole genome sequence information for a representative C. neteri strain indicated the presence of multiple open reading frames with sequence similarity to β-lactamases, including a chromosome-encoded AmpC β-lactamase and metallo-β-lactamases, consistent with the resistance phenotype of this bacterium. …”
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Global Gene Expression Profiling in PPAR-γ Agonist-Treated Kidneys in an Orthologous Rat Model of Human Autosomal Recessive Polycystic Kidney Disease by Daisuke Yoshihara, Masanori Kugita, Tamio Yamaguchi, Harold M. Aukema, Hiroki Kurahashi, Miwa Morita, Yoshiyuki Hiki, James P. Calvet, Darren P. Wallace, Takafumi Toyohara, Takaaki Abe, Shizuko Nagao

“…Their relevant pathways were identified using the Kyoto Encyclopedia of Gene and Genomes database. Stearoyl-coenzyme A desaturase 1 is a key enzyme in fatty acid metabolism found in the top 5 genes downregulated by PIO treatment. …”
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Comparative transcriptome analysis revealed gene expression differences in fruits between two Actinidia chinensis cultivars by Lina Guo, Kai Yan, Dan Li, Wanxing Li

“…Meanwhile, 1,915 could be aligned to the GO (Gene Ontology), 551 could be aligned to the KEGG (Kyoto Encyclopedia of Genes and Genomes Pathway), and 2,187 could be aligned to SWISS-PROT (SWISS-PROT Protein Sequence) database, respectively. …”
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The Impact of Interferon Lambda 3 Gene Polymorphism on Natural Course and Treatment of Hepatitis C by F. Bellanti, G. Vendemiale, E. Altomare, G. Serviddio

“…Very recently, three landmark genome-wide association studies identified single nucleotide polymorphisms near the interleukin 28B (IL28B) region which were more frequent in responders to treatment. …”
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Characterization of Halorubrum sfaxense sp. nov., a New Halophilic Archaeon Isolated from the Solar Saltern of Sfax in Tunisia by Hana Trigui, Salma Masmoudi, Céline Brochier-Armanet, Sami Maalej, Sam Dukan

“…The G+C content of the genomic DNA was 65.1 mol% (T(m)). Strain ETD6 grew in 15–35% (w/v) NaCl. …”
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PCR-Based Identification of Oral Streptococcal Species by Jeffrey A. Banas, Min Zhu, Deborah V. Dawson, Huojun Cao, Steven M. Levy

“…These novel primer sets offer a convenient means of presumptive identification that will have utility in many studies where large scale, in-depth genomic analyses are not practical.…”
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Genetic Diversity Analysis of Sugarcane Parents in Chinese Breeding Programmes Using gSSR Markers by Qian You, Liping Xu, Yifeng Zheng, Youxiong Que

“…In the present study, 115 sugarcane genotypes used for parental crossing were genotyped based on five genomic simple sequence repeat marker (gSSR) loci and 88 polymorphic alleles of loci (100%) as detected by capillary electrophoresis. …”
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XGBoost-enhanced ensemble model using discriminative hybrid features for the prediction of sumoylation sites by Salman Khan, Sumaiya Noor, Tahir Javed, Afshan Naseem, Fahad Aslam, Salman A. AlQahtani, Nijad Ahmad

“…Abstract Posttranslational modifications (PTMs) are essential for regulating protein localization and stability, significantly affecting gene expression, biological functions, and genome replication. Among these, sumoylation a PTM that attaches a chemical group to protein sequences—plays a critical role in protein function. …”
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Parity-Dependent Hemosiderin and Lipofuscin Accumulation in the Reproductively Aged Mouse Ovary by Ulises Urzua, Carlos Chacon, Renato Espinoza, Sebastián Martínez, Nicole Hernandez

“…In addition, the 8-OHdG adduct was evaluated in ovarian genomic DNA. Both hemosiderin and lipofuscin were significantly higher in virgin compared to multiparous ovaries. …”
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Directed Evolution of Microbial Communities in Fermented Foods: Strategies, Mechanisms, and Challenges by Zihan Yao, Ting Xie, Hongjie Deng, Shuzhi Xiao, Tao Yang

“…Additionally, we explore the integration of genomic tools and dynamic community analysis to understand and guide the evolutionary trajectories of these communities. …”
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SARS-COV-2 viral RNA detection through oligonucleotide-capped nanoporous anodic alumina supports. by Alba López-Palacios, María Nieves Aranda, Isabel Caballos, Andy Hernández-Montoto, Eva Calabuig, María Dolores Gómez-Ruiz, María Ángeles Tormo-Mas, Javier Pemán, Félix Sancenón, Ramón Martínez-Máñez, Elena Aznar, Estela Climent

“…The system is based on NAA that contains a fluorescent dye (Rhodamine B; RhB) and is capped with an oligonucleotide sequence that hybridize specifically a region of SARS-CoV-2 genome. In the presence of RNA from SARS-COV-2 virus, the oligonucleotide of the surface is displaced, uncapping the pores, and producing a delivery of RhB. …”
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Regulation of viral replication by host restriction factors by Ying Lin, Ying Lin, Ying Lin, Yun Zhu, Yun Zhu, Ling Jing, Ling Jing, Ling Jing, Xiaobo Lei, Xiaobo Lei, Zhengde Xie, Zhengde Xie

“…These cellular proteins inhibit viral replication and spread by impeding essential steps in the viral life cycle, such as viral entry, genome transcription and replication, protein translation, viral particle assembly, and release. …”
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Self-Renewal Signalling in Presenescent Tetraploid IMR90 Cells by Anda Huna, Kristine Salmina, Elina Jascenko, Gunars Duburs, Inna Inashkina, Jekaterina Erenpreisa

“…Endopolyploidy and genomic instability are shared features of both stress-induced cellular senescence and malignant growth. …”
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Gene Technology for Papaya Ringspot Virus Disease Management by Md. Abul Kalam Azad, Latifah Amin, Nik Marzuki Sidik

“…This review discusses the genome and the genetic diversity of PRSV, host range determinants, molecular diagnosis, disease management strategies, the development of transgenic papaya, environmental issues, issues in the adoption of transgenic papaya, and future directions for research.…”
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Hypoxia-Induced Reactive Oxygen Species: Their Role in Cancer Resistance and Emerging Therapies to Overcome It by Eleicy Nathaly Mendoza, Maria Rosa Ciriolo, Fabio Ciccarone

“…Hypoxia-induced ROS, resulting from mitochondrial dysfunction and enzyme activation, exacerbates genomic instability, tumor aggressiveness, and therapy resistance. …”
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