High throughput single cell metagenomic sequencing with semi-permeable capsules: unraveling microbial diversity at the single-cell level in sewage and fecal microbiomes by Meilee Ling, Judit Szarvas, Vaida Kurmauskaitė, Vaidotas Kiseliovas, Rapolas Žilionis, Rapolas Žilionis, Baptiste Avot, Baptiste Avot, Patrick Munk, Frank M. Aarestrup

“…Our method involves extracting and amplifying single bacterial DNA within individual SPCs, followed by combinatorial split-and-pool single-amplified genome (SAG) barcoding and short-read sequencing. …”
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Bacterial community assembly of specific pathogen-free neonatal mice by Elizabeth A. Kennedy, James S. Weagley, Andrew H. Kim, Avan Antia, Anna L. DeVeaux, Megan T. Baldridge

“…Short-read shotgun sequencing-based metagenomic profiling of young pups enabled the assembly of metagenome-assembled genomes for strain-level analysis of these pioneer Ligilactobacillus, Streptococcus, and Proteus species. …”
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Identification and characterization of Faecalibacterium prophages rich in diversity-generating retroelements by Anastasia Gulyaeva, Lei Liu, Sanzhima Garmaeva, Marloes Kruk, Rinse K. Weersma, Hermie J. M. Harmsen, Alexandra Zhernakova

“…One promising method of obtaining novel phages for experimental characterization is through induction of the prophages integrated into the genomes of cultured gut bacteria. Here, we developed a bioinformatic approach to prophage identification that builds on prophage genomic properties, existing prophage-detecting software, and publicly available virome sequencing data. …”
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Virulence factor discovery identifies associations between the Fic gene family and Fap2+ fusobacteria in colorectal cancer microbiomes by Geicho Nakatsu, Duhyun Ko, Monia Michaud, Eric A. Franzosa, Xochitl C. Morgan, Curtis Huttenhower, Wendy S. Garrett

“…Advances in omics analyses provide an opportunity to profile and identify microbial genomic features that correlate with the cancer-associated bacterial virulence factor Fap2. …”
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Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia by Claudio Azzolini, Simone Donati, Giovanni Micheloni, Vittoria Moretti, Roberto Valli, Francesco Acquati, Lucy Costantino, Fulvio Ferrara, Davide Borroni, Elias Premi, Francesco Testa, Francesca Simonelli, Giovanni Porta

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Evaluating the Influence of Hashimoto’s Thyroiditis on Clinico-Pathological Characteristics and Prognostic Outcomes of Middle Eastern Differentiated Thyroid Carcinoma by Sandeep Kumar Parvathareddy, Abdul K. Siraj, Nabil Siraj, Saeeda O. Ahmed, Saif S. Al-Sobhi, Fouad Al-Dayel, Khawla S. Al-Kuraya

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Maximising informativeness for target capture-based phylogenomics in Erica (Ericaceae) by Seth D. Musker, Nicolai M. Nürk, Michael D. Pirie

“…Plant phylogenetics has been revolutionised in the genomic era, with target capture acting as the primary workhorse of most recent research in the new field of phylogenomics. …”
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Long Noncoding RNAs, Chromatin, and Development by Daniel P. Caley, Ryan C. Pink, Daniel Trujillano, David R. F. Carter

“…The way in which the genome of a multicellular organism can orchestrate the differentiation of trillions of cells and many organs, all from a single fertilized egg, is the subject of intense study. …”
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Alzheimer’s disease may develop from changes in the immune system, cell cycle, and protein processing following alterations in ribosome function by Akiko Yamakawa, Mutsumi Suganuma, Risa Mitsumori, Shumpei Niida, Kouichi Ozaki, Daichi Shigemizu

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Muscle Satellite Cells: Exploring the Basic Biology to Rule Them by Camila F. Almeida, Stephanie A. Fernandes, Antonio F. Ribeiro Junior, Oswaldo Keith Okamoto, Mariz Vainzof

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Genetics of Keratoconus: Where Do We Stand? by Khaled K. Abu-Amero, Abdulrahman M. Al-Muammar, Altaf A. Kondkar

“…Keratoconus has a complex multifactorial etiology, with environmental, behavioral, and multiple genetic components contributing to the disease pathophysiology. Using genome-wide and candidate gene approaches several genomic loci and genes have been identified that highlight the complex molecular etiology of this disease. …”
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Relationships between the menstrual cycle and neuropsychiatric and physical symptoms in females with Tourette syndrome by Daisy T. Noriega-Makarskyy, Evan Realbuto, Ariadne Kaylor, Lisa Osiecki, Angela Essa, Dongmei Yu, Dongmei Yu, Cornelia Illmann, Carol A. Mathews, Carol A. Mathews, Carol A. Mathews, Jeremiah M. Scharf, Jeremiah M. Scharf, Jeremiah M. Scharf, Jeremiah M. Scharf, Jeremiah M. Scharf

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Middle-age cerebral small vessel disease and cognitive function in later life: a population-based prospective cohort studyResearch in context by Ali Tanweer Siddiquee, Yoon Ho Hwang, Soriul Kim, Sung Jin Shin, Ji Soo Lee, June Christoph Kang, Min-Hee Lee, Hyeon Jin Kim, Seung Ku Lee, Chol Shin

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Replication timing in Drosophila and its peculiarities in polytene chromosomes by T. D. Kolesnikova, O. V. Antonenko, I. V. Makunin

“…High-throughput methods allowed analysis of DNA replication on a genome scale, as well as its correlation with chromatin structure and gene activi ty. …”
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Insights into the origin, hybridisation and adaptation of Candida metapsilosis hybrid pathogens. by Valentina Del Olmo, Álvaro Redondo-Río, Alicia Benavente García, Savitree Limtong, Ester Saus, Toni Gabaldón

“…However, little is known about the genomic implications of hybridisation nor its impact on pathogenicity-related traits. …”
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Molecular cytological analysis of alien introgressions in common wheat lines created by crossing of <i>Triticum aestivum</i> with <i>T. dicoccoides</i> and <i>T. dicoccum</i> by О. A. Orlovskaya, I. N. Leonova, L. A. Solovey, N. I. Dubovets

“…A molecular cytogenetic analysis of introgression wheat lines indicated that the inclusion of the genetic material of wild and domesticated emmer was carried out mainly in the form of whole arms or large fragments in the chromosomes of the B genome and less extended inserts in the A genome. …”
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Are You My Host? An Overview of Methods Used to Link Bacteriophages with Hosts by Paul Hyman

“…These include methods to show phage genome entry into host cells (e.g., PhageFISH); proximity of phage and host genomes (e.g., proximity ligation, polonies, viral tagging); and analysis of genomes and metagenomes (e.g., CRISPR spacer analysis, metagenomic co-occurrence). …”
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Chromosome distribution of four LTR retrotransposons and 18 S rDNA in coffea eugenioides by Mariana Cansian Sattler, Jéssica Coutinho Silva, Stéfanie Cristina Oliveira, Wellington Ronildo Clarindo

“…Understanding the genomic organization and dynamics of the repeatome is fundamental to enlighten their role in plant genome evolution. …”
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Hf-induced strengthening and lattice distortion in HfxNbTaTiV refractory multi-principal element alloys by Yingwu Wang, Kai Xiong, Wei Li, Chengchen Jin, Haijun Wu, Hua Dai, Zihang Yang, Hongmo Yang, Shunmeng Zhang

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National Thalassemia Registry: a 30 year journey of implementing carrier screening in Singapore by Stella Zhang, Guek Peng Tan, Soh Lan Peh, Swee Lim Ang, Sylvia Kam, Weng Khong Lim, Teck Wah Ting, Angeline Lai, Ee Shien Tan, Saumya Shekhar Jamuar, Hai Yang Law, Ivy Ng

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