Characterization and phylogenetic analysis of the chloroplast genome of endangered Impatiens chishuiensis by LAN Wenxiang, MO Qing, HUANG Haiquan, HUANG Meijuan

“…[Methods] Based on the chloroplast genome sequence of I. chishuiensis, an endemic species in China, the assembly, annotation, gene characterization, sequence repetition and phylogenetic analysis of chloroplast genome were performed by bioinformatic softwares. …”
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A high-quality chromosome-scale genome assembly of the Cherokee rose (Rosa laevigata) by Yi Wang, Huijun Yan, Xianqin Qiu, Hao Zhang, Yonghong Zhang, Hongying Jian

“…A total of 493.2 Mb (99.8%) of the draft genome sequences were anchored on seven pseudochromosomes and two gapless pseudochromosomes were included in the final genome assembly. …”
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CysQ of , a Protozoa, May Have Been Acquired from Bacteria by Horizontal Gene Transfer by Ji Young Lee, Sangsoo Kim

“…Cryptosporidium parvum is a parasitic protozoan that causes an infectious disease. Its genome sequencing reported 14 bacteria-like proteins in the nuclear genome. …”
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Genome Resequencing of the Honeybee Apis mellifera jemenetica (Hymenoptera: Apidae): A Key Tool towards Characterization, Conservation, and Genomic Selection by Ahmad Alghamdi, Yehya Alattal

“…We report the whole-genome sequence of the Arabian honeybee (Apis mellifera jemenetica). …”
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Evidence of High-Risk Human Papillomavirus in Esophageal Cancer in East Azerbaijan Province, Northwest of Iran by Zahra Sadeghian, Hossein Bannazadeh Baghi, Vahdat Poortahmasebi, Javid Sadeghi, Alka Hasani, Arezoo Azadi, Mahin Ahangar Oskouee

“…In contrast, all cases of the control group were negative for the HPV genome. Sequence analysis revealed that HPV types 16 and 18 are the most frequent ones identified in this study. …”
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Confirming Multiplex RT-qPCR Use in COVID-19 with Next-Generation Sequencing: Strategies for Epidemiological Advantage by Rob E. Carpenter, Vaibhav Tamrakar, Harendra Chahar, Tyler Vine, Rahul Sharma

“…All 78 samples were classified into WHO lineages by whole-genome sequencing and then compared with two commercially available RT-qPCR assays for spike protein mutation(s). …”
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Mutations in Mycobacterium tuberculosis Isolates with Discordant Results for Drug-Susceptibility Testing in Peru by L. Solari, D. Santos-Lazaro, Z. M. Puyen

“…Our objective was to identify mutations that could explain discordant results in the evaluation of susceptibility to rifampicin and isoniazid between molecular and phenotypic methods, using whole genome sequencing (WGS). Peruvian strains showing sensitive results in the GenoType MTBDRplus v2.0 test and resistant results in the proportions in the agar-plaque test for isoniazid or rifampin were selected. …”
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Comparative genomics reveal a novel phylotaxonomic order in the genus Fusobacterium by Cristian Molteni, Diego Forni, Rachele Cagliani, Manuela Sironi

“…Thus, evidence herein calls for further evolutionary and phylogenomic analyses based on more Flavobacterium nucleatum genome sequences. More generally, our data inform future molecular profiling approaches to investigate the epidemiology of Fusobacterium-associated diseases.…”
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Identification of Viral Taxon-Specific Genes (VTSG): Application to by Shinduck Kang, Young-Chang Kim

“…With the development of genome sequence analysis technology, there is an increasing demand for virus taxonomy to be extended from in vivo and in vitro to in silico. …”
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First report of the chloroplast and mitochondrial genomes of the Indian pitcher plant, Nepenthes khasiana Hook.f. by Ruchishree Konhar, Debasis Dash, Devendra Kumar Biswal

“…These organelle genomes were assembled as part of a whole-genome sequencing project aimed at gaining deeper insights into their evolutionary relations with genomes of other carnivorous plants. …”
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Nucleosome patterns in four plant pathogenic fungi with contrasted genome structures by Clairet, Colin, Lapalu, Nicolas, Simon, Adeline, Soyer, Jessica L., Viaud, Muriel, Zehraoui, Enric, Dalmais, Berengère, Fudal, Isabelle, Ponts, Nadia

“…Our findings support that nucleosome positioning and occupancies are subjected to evolution, in relation with underlying genome sequence modifications. Understanding genomic organization and its role in expression regulation is the next gear to understand complex cellular mechanisms and their evolution. …”
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Research note: A low-density SNP genotyping panel for Chinese native chickens by Jie Wang, Siyuan Xing, Yan Zhou, Jie Liu, Yan Sun, Qiuxia Lei, Haixia Han, Wei Liu, Dapeng Li, Fuwei Li, Dingguo Cao

“…The panel design was based on whole genome sequencing data from 7 local breeds and a commercial breed. …”
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Validation of Customized Cancer Panel for Detecting Somatic Mutations and Copy Number Alterations by Su-Hye Choi, Seung-Hyun Jung, Yeun-Jun Chung

“…With targeted sequencing, we can obtain deeper coverage of reads and handle data more easily with a relatively lower cost and less time than whole-exome or whole-genome sequencing. Recently, we designed a customized gene panel for targeted sequencing of major solid cancers. …”
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Structural and Functional Annotation of Hypothetical Proteins of O139 by Md. Saiful Islam, Shah Md. Shahik, Md. Sohel, Noman I. A. Patwary, Md. Anayet Hasan

“…Vibrio cholerae is one of the responsible bacteria involved in cholera disease. The complete genome sequence of V. cholerae deciphers the presence of various genes and hypothetical proteins whose function are not yet understood. …”
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Assembly-free reads accurate identification (AFRAID) approach outperforms other methods of DNA barcoding in the walnut family (Juglandaceae) by Yanlei Liu, Kai Chen, Lihu Wang, Xinqiang Yu, Chao Xu, Zhili Suo, Shiliang Zhou, Shuo Shi, Wenpan Dong

“…Species identification of 100% was achieved when chloroplast genome sequence coverage reached 20% and the original sequencing data reached 500,000 reads. …”
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Post-mortem and histological lesions in the European minks (Mustela lutreola) induced by spontaneous infection with coronavirus SARS-CoV-2 by I. A. Subbotina, I. N. Gromov

“…Profound and detailed studies of the virus circulation in the European mink population and the clinical manifestations of the disease, comprehensive approaches to the disease diagnosis, including epidemiological studies, clinical and post-mortem examinations, molecular genetic laboratory diagnostics (polymerase chain reaction and full-genome sequencing) contributed to better understanding of the disease features. …”
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Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, Iraq by Sura S. Aghwan, Enas S. Hussein, Salam A Esmaeel

“…The results indicate that some of the tested samples contain DNA from the Cytauxzoon genus, based on the presence of bands at the expected 1600 bp size. The local genome sequence of Cytauxzoon felis Sesa LC775709.1 is highly similar to sequences found in geographically diverse regions such as Switzerland, Italy, France, and the USA. …”
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Cancer phylogenetic tree inference at scale from 1000s of single cell genomes by Salehi, Sohrab, Dorri, Fatemeh, Chern, Kevin, Kabeer, Farhia, Rusk, Nicole, Funnell, Tyler, Williams, Marc J., Lai, Daniel, Andronescu, Mirela, Campbell, Kieran R., McPherson, Andrew, Aparicio, Samuel, Roth, Andrew, Shah, Sohrab P., Bouchard-Côté, Alexandre

“…A new generation of scalable single cell whole genome sequencing (scWGS) methods allows unprecedented high resolution measurement of the evolutionary dynamics of cancer cell populations. …”
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Human Genomic Loci Important in Common Infectious Diseases: Role of High-Throughput Sequencing and Genome-Wide Association Studies by Gerald Mboowa, Ivan Sserwadda, Marion Amujal, Norah Namatovu

“…High-throughput sequencing (HTS) has transformed both the management of infectious diseases and continues to enable large-scale functional characterization of host resistance/susceptibility alleles and loci; a paradigm shift from single candidate gene studies. Application of genome sequencing technologies and genomics has enabled us to interrogate the host-pathogen interface for improving human health. …”
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SARS-CoV-2 Remained Airborne for a Prolonged Time in a Lockdown Confined Space by Xinyue Li, Xiao Qi, Jianxin Ma, Yang Pan, Tian Tian, Yue Zhang, Zhen Li, Wenjing Li, Lingli Sun, Lu Zhang, Zheng Zhang, Quanyi Wang, Maosheng Yao

“…The traveler did not have any contacts with the two employees; however, genome sequencing showed that SARS-CoV-2 variants from three patients and two environmental surface samples belonged to 20B viral clade, sharing a nucleic acid identity of more than 99.9%. …”
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