Pantoea eucrina: Catheter‐Related Bloodstream Infection in a Woman With Short Bowel Syndrome by Ida Saksenborg Kølle, Lars Vinter‐Jensen, Morten Eneberg Nielsen, Hans Linde Nielsen

“…Despite presenting with only minor clinical symptoms, blood cultures from both central and peripheral sites confirmed the presence of P. eucrina, identified by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) and whole‐genome sequencing. The infection was successfully cleared following a 13‐day course of gentamicin lock therapy and intravenous piperacillin–tazobactam, after which the catheter was removed.…”
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Moving “Forward” in Plasmodium Genetics through a Transposon-Based Approach by Bharath Balu

“…The genome sequence of the human malaria parasite, Plasmodium falciparum, was released almost a decade ago. …”
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IMA Genome – F19 by Janneke Aylward, Andi M. Wilson, Cobus M. Visagie, Joseph Spraker, Irene Barnes, Carla Buitendag, Callin Ceriani, Lina Del Mar Angel, Deanné du Plessis, Taygen Fuchs, Katharina Gasser, Daniella Krämer, WenWen Li, Kiara Munsamy, Anja Piso, Jenna-Lee Price, Byron Sonnekus, Chanel Thomas, Ariska van der Nest, Alida van Dijk, Alishia van Heerden, Nicole van Vuuren, Neriman Yilmaz, Tuan A. Duong, Nicolaas A. van der Merwe, Michael J. Wingfield, Brenda D. Wingfield

“…In this issue, we report on a Genome Assembly Workshop for Mycologists that was held at the Forestry and Agricultural Biotechnology Institute (FABI) at the University of Pretoria, South Africa and make available the 12 draft genome sequences emanating from the event. With the aim of making the process of genome assembly and annotation more accessible to biologists, we provide a step-by-step guide to both genome assembly and annotation, intended to encourage and empower mycologists to use genome data in their research.…”
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Introduction to International Ethical Standards Related to Genetics and Genomics by Seon-Hee Yim, Yeun-Jun Chung

“…After that, the morality of one recent event related to whole-genome sequencing and person-identifiable genetic data will be explored based on those international standards.…”
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RNA splicing: a split consensus reveals two major 5′ splice site classes by Matthew T. Parker, Sebastian M. Fica, Gordon G. Simpson

“…The two 5′ splice site classes are separable in genome sequences, sensitized by specific genotypes and associated with splicing complexity. …”
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Ophiostomatoid fungi associated with pine bark beetles and infested pines in south-eastern Australia, including Graphilbum ipis-grandicollis sp. nov. by Conrad Trollip, Angus J. Carnegie, Quang Dinh, Jatinder Kaur, David Smith, Ross Mann, Brendan Rodoni, Jacqueline Edwards

“…This study also provides an early example of whole genome sequencing (WGS) approaches replacing traditional PCR-based methods for taxonomic surveys. …”
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On the nature of picobirnaviruses by A. Yu. Kashnikov, N. V. Epifanova, N. A. Novikova

“…The reasons for the occurrence of atypical sequences among the PBV genome sequences that use an alter­ native mitochondrial code of lower eukaryotes (fungi and invertebrates) for the translation of viral RNA­dependent RNA polymerase (RdRp) instead of the standard genetic code are analyzed. …”
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Genomic characterization of three marine fungi, including Emericellopsis atlantica sp. nov. with signatures of a generalist lifestyle and marine biomass degradation by Ole Christian Hagestad, Lingwei Hou, Jeanette H. Andersen, Espen H. Hansen, Bjørn Altermark, Chun Li, Eric Kuhnert, Russell J. Cox, Pedro W. Crous, Joseph W. Spatafora, Kathleen Lail, Mojgan Amirebrahimi, Anna Lipzen, Jasmyn Pangilinan, William Andreopoulos, Richard D. Hayes, Vivian Ng, Igor V. Grigoriev, Stephen A. Jackson, Thomas D. S. Sutton, Alan D. W. Dobson, Teppo Rämä

“…These species were genome sequenced to study their genomic features, biosynthetic potential and phylogenetic placement using multilocus data. …”
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Genomic and Pathogenic Characteristics of Virulent Newcastle Disease Virus Isolated from Chicken in Live Bird Markets and Backyard Flocks in Kenya by Irene N. Ogali, Paul O. Okumu, Erick O. Mungube, Jacqueline K. Lichoti, Stephen Ogada, Grace K. Moraa, Bernard R. Agwanda, Sheila C. Ommeh

“…This study reports the first complete genome sequences of NDV from backyard chicken in Kenya. …”
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Fleming's penicillin producing strain is not Penicillium chrysogenum but P. rubens by J. Houbraken, J.C. Frisvad, R.A. Samson

“…Fleming's original penicillin producing strain and the full genome sequenced strain of P. chrysogenum are re-identified as P. rubens. …”
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Meningococcal B Vaccination (4CMenB) in Infants and Toddlers by Susanna Esposito, Claudia Tagliabue, Samantha Bosis

“…Serogroup B has long been a challenge but the discovery of the complete genome sequence of an MenB strain has allowed the development of a specific four-component vaccine (4CMenB). …”
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Comparative Analysis of Predicted Gene Expression among Crenarchaeal Genomes by Shibsankar Das, Brajadulal Chottopadhyay, Satyabrata Sahoo

“…Research into new methods for identifying highly expressed genes in anonymous genome sequences has been going on for more than 15 years. …”
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Transposable Elements: No More 'Junk DNA' by Yun-Ji Kim, Jungnam Lee, Kyudong Han

“…Since the advent of whole-genome sequencing, transposable elements (TEs), just thought to be 'junk' DNA, have been noticed because of their numerous copies in various eukaryotic genomes. …”
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ICGenomics: A PROGRAM COMPLEX FOR ANALYSIS OF SYMBOL SEQUENCES IN GENOMICS by Y. L. Orlov, A. O. Bragin, I. V. Medvedeva, K. V. Gunbin, P. S. Demenkov, O. V. Vishnevsky, V. G. Levitsky, D. Y. Oshchepkov, N. L. Podkolodnyy, D. A. Afonnikov, I. Grosse, N. A. Kolchanov

“…Application of ICGenomics to the analysis of genomic sequences of the parasite Opisthorchis felineus and to ChIP-seq data on the mouse and human is considered. …”
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Successful Diagnosis of Sengers Syndrome Using a Comprehensive Genomic Analysis by Kohta Nakamura, Yukiko Yatsuka, Sachie Naito, Akira Hasegawa, Takeya Kasukawa, Atsushi Kondo, Yoshihito Kishita, Yohei Sugiyama, Takanori Onuki, Tomohiro Ebihara, Tomoko Tsuruoka, Takuya Fushimi, Akira Ohtake, Kei Murayama, Atsuko Imai‐Okazaki, Yasushi Okazaki

“…Methods We employed a comprehensive genomic analysis approach, including whole‐genome sequencing and RNA sequencing, combined with various bioinformatics tools. …”
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Sanger validation of WGS variants by Arina Kopernik, Mariia Sayganova, Gaukhar Zobkova, Natalia Doroschuk, Anna Smirnova, Daria Molodtsova-Zolotukhina, Olesya Sagaydak, Oxana Ryzhkova, Sergey Kutsev, Olga Groznova, Lyusya Melikyan, Elizaveta Bondarchuk, Mary Woroncow, Eugene Albert, Viktor Bogdanov, Pavel Volchkov

“…Despite that, no works to date report the concordance between variants from whole genome sequencing and their gold-standard Sanger validation. …”
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Alternative Splicing in Self-Renewal of Embryonic Stem Cells by Clara Y. Cheong, Thomas Lufkin

“…Progressing beyond the limitations of a fixed genomic sequence, alternative splicing offers an additional layer of complexity to produce protein variants that may differ in function and localization that can direct embryonic stem cells to specific differentiation pathways. …”
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A Novel Partial Sequence Alignment Tool for Finding Large Deletions by Taner Aruk, Duran Ustek, Olcay Kursun

“…Finding large deletions in genome sequences has become increasingly more useful in bioinformatics, such as in clinical research and diagnosis. …”
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Development of Primer Panels for Whole-Genome Amplification and Sequencing of Human Seasonal Coronaviruses: hCoV-OC43, hCoV-HKU1, hCoV-229E, and hCoV-NL63 by Tamila Musaeva, Artem Fadeev, Maria Pisareva, Veronika Eder, Andrey Ksenafontov, Margarita Korzhanova, Valery Tsvetkov, Alexander Perederiy, Irina Kiseleva, Daria Danilenko, Dmitry Lioznov, Andrey Komissarov

“…In this study, we developed a two-pool, long-amplicon (900–1100 bp) PCR primer panel for the whole-genome sequencing of four seasonal hCoV species. The panel was validated using nasopharyngeal swab samples collected within the Global Influenza Hospital Surveillance Network (GIHSN) project. …”
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Coleopteran Antimicrobial Peptides: Prospects for Clinical Applications by Monde Ntwasa, Akira Goto, Shoichiro Kurata

“…Recently, the complete genome sequence of Tribolium was published, boosting research on coleopteran immunity and leading to the identification of Tribolium AMPs that are shared by Drosophila and mammals, as well as other AMPs that are unique. …”
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