Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The objects of the study were recombinant clones of Komagataella phaffii K51 carrying the heterologous proteinase K (PK-w) gene from Tritirachium album integrated into their genome as well as samples of recombinant proteinase K isolated from these clones. …”
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Comparative cytogenetics of anembryonic pregnancies and missed abortions in human by T. V. Nikitina, E. A. Sazhenova, E. N. Tolmacheva, N. N. Sukhanova, S. A. Vasilyev, I. N. Lebedev

“…Thus, although the frequencies of some types of chromosomal pathology differ between AP and MA, the total frequency of chromosomal abnormalities in AP is not increased compared to MA, which indicates the need to search for the causes of AP at other levels of the genome organization, including microstructural chromosomal rearrangements, monogenic mutations, imprinting disorders, and epigenetic abnormalities.…”
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Comprehensive analysis of mRNA and miRNA differential expression profiles in the hypothalamus-pituitary-gonadal axis in laying and broodiness period of Wanxi white geese by Xinwei Tong, Xiaojin Li, Yuhua Wang, Fei Xie, Ruidong Li, Man Ren, Qianqian Hu, Shenghe Li

“…Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analysis showed that differentially expressed genes (DEGs) and differentially expressed miRNAs (DEMs) were significantly enriched in hormone level regulation, cell communication, calcium signaling pathway, GnRH signaling pathway, MAPK signaling pathway, Wnt signaling pathway, and other processes. …”
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Deep learning–based assessment of missense variants in the COG4 gene presented with bilateral congenital cataract by Shaohua Zhang, Yang Sun, Yinghong Ji, Li Ning, Binghe Xiao, Maierdanjiang Ainiwaer, Houyi Liu, Yingying Hong

“…Despite classified as VUS (American College of Medical Genetics and Genomics criteria: PM1, PP4), the pathogenicity in this Cog4 variant cannot be ruled out and warrants further investigation.…”
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Exploring the functional role of POU5F1 using CRISPR RNP electroporation of zygote (CRISPR-EZ) in buffaloes by Meeti Punetha, Dharmendra Kumar, Suman Choudhary, Sheetal Saini, Kamlesh Kumari Bajwa, Surabhi Sharma, Manu Mangal, P. S. Yadav, T. K. Datta

“…We developed an easy and straightforward protocol for gene editing, which could serve as a valuable method for studying the functional genomics of the buffalo embryos. …”
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HIV-1 drug resistance and genetic transmission networks among patients with sexually transmitted HIV in Ningxia, China by Jianxin Pei, Zhonglan Wu, Zhonglan Wu, Bingqian Si, Chunhua Ma, Yichang Liu, Xiaofa Ma, Wenhe Kuai, Yinhao Zhang, Yong Li

“…This study was conducted to better understand the relationship between genotypes, drug resistance, and molecular transmission networks in two groups of sexually transmitted HIV-1 in Ningxia, China.MethodsPlasma samples were collected from sexually transmitted HIV/AIDS patients in Ningxia between 2020 and 2021 for RNA extraction followed by HIV-1 genome sequencing, genotype and drug resistance analyses. …”
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Elevated POSTN expression predicts poor prognosis and is associated with radioresistance in cervical cancer patients treated with radical radiotherapy by Cui-qin Huang, Wen-tao Xiao, Xiang-rong Yao, Zhi-min Li, Jun-yan He

“…We analyzed data from 92 CC patients in The Cancer Genome Atlas (TCGA) and 153 patients from our institution, assessing POSTN expression levels through mRNA analysis and immunohistochemistry (IHC). …”
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Development and experimental validation of dephosphorylation-related biomarkers to assess prognosis and immunotherapeutic response in gliomas by Hui Tang, Xuping Yang, Xuping Yang, Guoqian Li, Ke Peng, Yang Sun, Longyang Jiang, Longyang Jiang, Yilan Huang, Yilan Huang

“…This study aims to construct a validated prognostic risk model for dephosphorylation, which will provide new directions for clinical treatment, prognostic assessment, and temozolomide (TMZ) resistance in glioma patients.MethodsScreening dephosphorylation-related genes (DRGs) and transcriptome expression data from The Cancer Genome Atlas (TCGA), Molecular signatures database (MSigDB) and constructing risk scoring models. …”
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Reductive acetogenesis is a dominant process in the ruminant hindgut by Qiushuang Li, Jiabin Huo, Gaofeng Ni, Fan Zhang, Shizhe Zhang, Xiumin Zhang, Rong Wang, Jinzhen Jiao, Zhongtang Yu, Xuanxuan Pu, Yipeng Yue, Emilio M. Ungerfeld, Xiaoli Zhang, Jian Wu, Zhiliang Tan, Chris Greening, Min Wang

“…Most strikingly, reductive acetogenic bacteria were 12-fold more abundant in the cecum. Genome-resolved metagenomic analysis unveiled that the cecum acetogens are both phylogenetically and functionally distinct from those found in the rumen. …”
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A prognostic signature of Glutathione metabolism-associated long non-coding RNAs for lung adenocarcinoma with immune microenvironment insights by Junxi Hu, Junxi Hu, Shuyu Tian, Shuyu Tian, Qingwen Liu, Qingwen Liu, Jiaqi Hou, Jiaqi Hou, Jun wu, Jun wu, Xiaolin Wang, Xiaolin Wang, Yusheng Shu, Yusheng Shu

“…This study developed a prognostic model using GSH-related lncRNAs to predict LUAD outcomes and assess tumor immunity.MethodsThis study analyzed survival data from The Cancer Genome Atlas (TCGA) and identified GSH metabolism-related lncRNAs using Pearson correlation. …”
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Mutations in the <i>A34R</i> gene increase the immunogenicity of vaccinia virus by S. N. Shchelkunov, T. V. Bauer, S. N. Yakubitskiy, A. A. Sergeev, A. S. Kabanov, S. A. Pyankov

“…The most effective agents are live vaccines, usually low-virulence organisms for humans and closely related to pathogenic viruses or attenuated as a result of mutations/deletions in the genome of pathogenic virus. Smallpox vaccination with live vaccinia virus (VACV) closely related to smallpox virus played a key role in the success of the global smallpox eradication program carried out under the World Health Organization auspices. …”
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Understanding the educational inequalities in suicide attempts and their mediators: a Mendelian randomisation study by Jing Wang, Ye Yang, Rui Gao, Lilu Ding, Jiahao Zhu, Dan Zhou, Yingjun Li, Houpu Liu

“…Elucidating modifiable risk factors that serve as intermediaries in the impact of low educational attainment on suicide has the potential to reduce health disparities.Aims To examine the risk factors that mediate the relationship between educational attainment and suicide attempts and quantify their contributions to the mediation effect.Methods We conducted a two-sample Mendelian randomisation (MR) analysis to estimate the causal effect of educational attainment on suicide attempts, utilising genome-wide association study summary statistics from the Integrative Psychiatric Research (iPSYCH; 6024 cases and 44 240 controls) and FinnGen (8978 cases and 368 299 controls). …”
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Prognostic values of intracellular cell-related genes in esophageal cancer and their regulatory mechanisms by Wei Cao, Dacheng Jin, Weirun Min, Haochi Li, Rong Wang, Jinlong Zhang, Yunjiu Gou

“…Hence, investigating the prognostic significance and regulatory mechanisms of genes related to these intracellular structures in esophageal cancer is imperative. The Cancer Genome Atlas (TCGA) Esophageal Cancer (ESCA) dataset served as the training set for the analysis. …”
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Antibiotic Resistance of Enterobacteriaceae in Microbiomes Associated with Poultry Farming by Anna S. Krivonogova, Irina M. Donnik, Albina G. Isaeva, Egor A. Loginov, Maxim V. Petropavlovskiy, Elisey N. Bespamyatnykh

“…During a broiler’s life span, bacteria with no active resistance determinants in their genome remained sensitive to antibiotics, even though the contact with the latter was constant. …”
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Assessing inflammatory protein factors in inflammatory bowel Disease using multivariable mendelian randomization by Qiang Su, Yun Lu, Song He, Jiang Liang, Song Huang, Yuanli He, Zhenxiang An

“…This study uses Mendelian randomization (MR) analysis to identify cytokines that may be associated with UC and CD, aiding in early diagnosis and treatment decisions. Methods Genome-wide association study (GWAS) data for inflammatory cytokine levels were obtained from a cohort of 14,824 individuals of European descent. …”
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Pheochromocytoma in Congenital Cyanotic Heart Disease by Carmen Aresta, Gianfranco Butera, Antonietta Tufano, Giorgia Grassi, Livio Luzi, Stefano Benedini

“…Studies on genome-wide transcription patterns have shown that many genetic alterations implicated in pheochromocytoma-paraganglioma (P-PGL) syndromes cluster in a common cellular pathway leading to aberrant activation of molecular response to hypoxia in normoxic conditions (the pseudohypoxia hypothesis). …”
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Phage WO diversity and evolutionary forces associated with Wolbachia-infected crickets by Qing-Chen Luo, Yue-Yuan Li, Ye-Song Ren, Xiao-Hui Yang, Dao-Hong Zhu

“…These findings suggest that horizontal transmission and intragenic recombination have played a significant role in the evolution of the phage WO genome, effectively enhancing the diversity of phage WO associated with crickets.…”
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Exploring the mediating role of blood metabolites in the relationship between gut microbiota and gastric cancer risk: a Mendelian randomization study by Xiaocheng Li, Xiaocheng Li, Huapeng Lin, Huapeng Lin, Huapeng Lin, Jing Peng, Jianping Gong

“…However, the causal nature of these associations remains uncertain.MethodsWe utilized summary data from genome-wide association studies (GWAS) on GM (European, n=8,956), blood metabolites (European, n=120,241; East Asian, n=4,435), and GC (European, n=476,116; East Asian, n=167,122) to perform a bidirectional Mendelian randomization (MR) analysis, investigating the causal effects of GM and metabolites on GC risk. …”
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Circle-seq reveals that eccDNA may be a key blood biomarker for HBV-associated liver cancer by Xiao K. Ran, Xiao F. Zhao, Zhen W. Wei, Hua Z. Pang, Yan F. Tang, Rong Liu, Tie X. Wu, Xu D. Liu

“…Geno Ontology enrichment and Kyoto Encyclopedia of Genes and Genome pathway analyses were performed on differentially expressed eccDNA-related genes. …”
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A ribodepletion and tagging protocol to multiplex samples for RNA-seq based virus detection: application to the cassava virome by Daniel H. Otron, Justin S. Pita, Murielle Hoareau, Fidèle Tiendrébéogo, Jean-Michel Lett, Pierre Lefeuvre

“…After confirming that the protocol was suitable for ribodepletion, we demonstrated it was possible to detect RNA and DNA viruses via identification of near full-size genomes. Additional phylogenetic analyses confirmed the presence of begomoviruses and ipomoviruses responsible for CMD and CBSD, respectively. …”
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