Antimicrobial susceptibility and epidemiological types of Legionella pneumophila human isolates from Italy (1987–2020) by Maria Scaturro, Alessio Lanni, Fabiola Mancini, Antonietta Girolamo, Silvia Fillo, Andrea Ciammaruconi, Florigio Lista, Clementina Elvezia Cocuzza, Rosario Musumeci, Christophe Ginevra, Ghislaine Descours, Sophie Jarraud, Jessica Iera, Paolo Visca, Maria Luisa Ricci

“…The presence of the lpeAB efflux pump genes was also investigated. The genome sequences of a subpopulation of isolates showing reduced susceptibility to azithromycin were also analysed. …”
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Decreased Spp1 Expression in Acute Myocardial Infarction after Ischemia and Reperfusion Injury by Ling Li, Jungang Huang, Zongkai Zhao, Zhuzhi Wen, Kang Li, Tianjiao Ma, Lisui Zhang, Junmeng Zheng, Shi Liang

“…In addition, Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to find the biological property of 449 RNAs from GSE83472 via the Database for Annotation, Visualization, and Integrated Discovery (DAVID). …”
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A positive experience in applying the biolistic approach to potato varieties Aksor and Nevskiy by N. P. Malakhova, Y. A. Skiba, G. A. Iskakova, D. A. Naizabayeva, B. K. Tezekbaeva, G. A. Ismagulova, E. R. Maltseva

“…The main advantages of this method include the ability to simultaneously incorporate several target genes into the plant genome, carry out transfer without unnecessary agrobacterial parts and plasmid DNA sequences, and the short time needed to produce transgenic cells. …”
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Investigation of the Mechanism of Complement System in Diabetic Nephropathy via Bioinformatics Analysis by Bojun Xu, Lei Wang, Huakui Zhan, Liangbin Zhao, Yuehan Wang, Meng Shen, Keyang Xu, Li Li, Xu Luo, Shasha Zhou, Anqi Tang, Gang Liu, Lu Song, Yan Li

“…Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were carried out to determine the molecular mechanisms of DEGs involved in the progression of DN. …”
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Establishment of a rapid method for the detection of Brucella canis based on recombinase-mediated thermostable nucleic acid amplification technology by Shao-Zheng Song, Zi-Yuan Li, Yuan-Yuan Liu, Ying-Chao Wu, Kang-Ying Yu, Zhengyi He

“…Specificity was tested using genomes from Brucella canis, Brucella suis, Brucella melitensis, Brucella abortus, Staphylococcus aureus, pathogenic Escherichia coli, Salmonella enteritidis, Shigella spp., Proteus mirabilis, and Listeria monocytogenes. …”
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Expression Analysis and Functional Validation of DcTPSb1 in Terpene Synthesis of <i>Dendrobium chrysotoxum</i> by Yuxuan Jin, Shuting Zhou, Zhihui Du, Weize Wang, Zhilin Chen

“…Analysis of the <i>D. chrysotoxum</i> genome and transcriptional data revealed that the gene <i>DcTPSb1</i> was significantly up-regulated during flowering periods, showing a strong correlation with the accumulation of aromatic monoterpenes in the floral components of <i>Dendrobium chrysotoxum</i>. …”
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Minimization of the Bacillus subtilis divisome suggests FtsZ and SepF can form an active Z-ring, and reveals the amino acid transporter BraB as a new cell division influencing fact... by Ilkay Celik Gulsoy, Terrens N V Saaki, Michaela Wenzel, Simon Syvertsson, Taku Morimoto, Tjalling K Siersma, Leendert W Hamoen

“…Viability of the multiple deletion mutant was not greatly affected, although the frequency of cell division was considerably reduced. Whole genome sequencing suggested that the construction of this minimal divisome strain was also possible due to the accumulation of suppressor mutations. …”
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Relationship between inherited genetic variation and survival from colorectal cancer stratified by tumour location by Christopher Wills, Katie Watts, Amy Houseman, Timothy S. Maughan, David Fisher, Nada A. Al-Tassan, Richard S. Houlston, Valentina Escott-Price, Jeremy P. Cheadle

“…We studied 1899 patients with advanced CRC (514 had proximal colonic, 493 distal colonic and 892 rectal tumours) and carried out genome-wide association studies for survival. Single nucleotide polymorphisms (SNPs) suggestive of association (P < 1.0 × 10–5) were tested for replication in 5078 CRC patients from the UK Biobank. …”
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Duckweed: a starch-hyperaccumulating plant under cultivation with a combination of nutrient limitation and elevated CO2 by Ling Guo, Ling Guo, Yang Fang, Songhu Wang, Yao Xiao, Yanqiang Ding, Yanling Jin, Xueping Tian, Anping Du, Zhihua Liao, Kaize He, Shuang Chen, Yonggui Zhao, Li Tan, Zhuolin Yi, Yuqing Che, Lanchai Chen, Jinmeng Li, Leyi Zhao, Peng Zhang, Zhengbiao Gu, Fangyuan Zhang, Yan Hong, Qing Zhang, Hai Zhao

“…In this study, we integrated whole-genome sequencing, epigenomics, transcriptomics, enzyme activity, and composition variation to elucidate the mechanisms of efficient starch accumulation in duckweed in terms of starch accumulation and carbon partitioning, regulation of the expression of genes in the starch metabolic pathway, and sucrose biosynthesis and transportation.Results and discussionAlthough Landoltia punctata exhibits dramatic gene family contraction, its starch content and productivity reached 72.2% (dry basis) and 10.4 g m-2 d-1, respectively, in 10 days, equivalent to a yield of 38.0 t ha-1 y-1, under nutrient limitation treatment with elevated CO2 levels. …”
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Effective resistance to powdery mildew in Aegilops L. accessions by M. A. Kolesova, N. N. Chikida, M. Kh. Belousova, L. G. Tyryshkin

“…As a result of the tests, susceptibility to powdery mildew was shown in all Aegilops accessions of the D-genome group; all the studied representatives of Ae. speltoides, Ae. caudata and Ae. biuncialis were highly resistant to powdery mildew.…”
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Comparative Metabolic Analysis of Different <i>Indica</i> Rice Varieties Associated with Seed Storability by Fangxi Wu, Yidong Wei, Yongsheng Zhu, Xi Luo, Wei He, Yingheng Wang, Qiuhua Cai, Huaan Xie, Guosheng Xie, Jianfu Zhang

“…The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that several biosynthesis pathways were involved in the observed metabolite differences, including alpha-linolenic acid metabolism, butanoate metabolism, and propanoate metabolism. …”
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Characterization of the Phosphotransferase from <i>Bacillus subtilis</i> 1101 That Is Responsible for the Biotransformation of Zearalenone by Yuzhuo Wu, Qiuyu Zhou, Junqiang Hu, Yunfan Shan, Jinyue Liu, Gang Wang, Yin-Won Lee, Jianrong Shi, Jianhong Xu

“…In this study, a ZEA phosphotransferase, ZPH1101, was identified from <i>Bacillus subtilis</i> 1101 using genome sequencing. The product transformed by ZPH1101 was identified as phosphorylated ZEA (ZEA-P) through LC-TOF-MS/MS analysis. …”
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Bidirectional Mendelian Randomization Analysis of the Association Between Mitochondrial Proteins and Neurodegenerative Diseases by Fangyuan Wang, Zhou Jing, Qingyi Wang, Minghe Li, Bingqi Lu, Ao Huo, Chenglin Zhao, Huanyu Zhou, Wulong Liang, Weihua Hu, Xudong Fu

“…Methods Herein, we utilized data from genome‐wide association studies (GWAS) on mitochondrial proteins and neurodegenerative diseases. …”
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Genetic insights into therapeutic targets for gout: evidence from a multi-omics mendelian randomization study by Mingyuan Fan, Zhangjun Yun, Jiushu Yuan, Sai Zhang, Hongyan Xie, Dingyi Lu, Haipo Yuan, Hong Gao

“…Methods A drug-targeted MR study was performed for gout by integrating the gout genome-wide association studies (GWAS) summary data and cis expression quantitative trait loci of 2,633 druggable genes from multiple cohorts. …”
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SARS-CoV-2 excretion and genetic evolution in nasopharyngeal and stool samples from primary immunodeficiency and immunocompetent pediatric patients by Haifa Khemiri, Ilhem Ben Fraj, Alessio Lorusso, Najla Mekki, Iolanda Mangone, Mariem Gdoura, Adriano Di Pasqual, Cesare Cammà, Valeria Di Lollo, Asma Cherni, Henda Touzi, Amel Sadraoui, Zina Meddeb, Nahed Hogga, Imen Ben Mustapha, Mohamed-Ridha Barbouche, Monia Ouederni, Henda Triki, Sondes Haddad-Boubaker

“…RNA detection was performed using RT-qPCR, and whole-genome sequencing was conducted with the NexSeq 1000 platform. …”
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Preimplantation genetic testing for four families with severe combined immunodeficiency: Three unaffected livebirths by Lingyun Zhang, Lei Feng, Hao Shi, Wenbin Niu, Yanchi Wang, Bei Bu, Yidong Liu, Xiao Bao, Wenyan Song, Haixia Jin, Yingpu Sun

“…The strategy of PGT-M started with a biopsy of the trophectoderm cells of embryos, and the whole genome was amplified by multiple replacement amplification (MDA). …”
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Newly isolated halotolerant Gordonia terrae S-LD serves as a microbial cell factory for the bioconversion of used soybean oil into polyhydroxybutyrate by Song Xu, Ruiqin Han, Lidan Tao, Zhipeng Zhang, Junfei Gao, Xinyuan Wang, Wei Zhao, Xiaoxia Zhang, Zhiyong Huang

“…In addition, the whole genome was sequenced, and the PHB biosynthetic pathway and quantitative expression of key genes were delineated in the novel isolated strain. …”
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Chikungunya virus infection in the skin: histopathology and cutaneous immunological response by Natália Gedeão Salomão, Natália Gedeão Salomão, Luciana Araújo, Luiz José de Souza, Anna Luiza Young, Carlos Basílio-de-Oliveira, Rodrigo Panno Basílio-de-Oliveira, Jorge José de Carvalho, Priscilla Conrado Guerra Nunes, Juliana Fernandes da Silva Amorim, Douglas Valiati dos Santos Barbosa, Marciano Viana Paes, Kíssila Rabelo, Kíssila Rabelo, Flavia Dos Santos

“…Since the skin is the first line of defense against CHIKV infection, in this study, we aimed to investigate the immunohistopathological aspects of the skin of infected individuals during the acute phase of the disease by performing histopathological and ultrastructural analysis, detection and quantification of the viral genome, detection of viral antigen and immune cells, and cytokines/chemokines’ characterization. …”
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Diagnosis of invasive encapsulated follicular variant papillary thyroid carcinoma by protein-based machine learning by Truong Phan-Xuan Nguyen, Minh-Khang Le, Sittiruk Roytrakul, Shanop Shuangshoti, Nakarin Kitkumthorn, Somboon Keelawat

“…Additionally, we analyzed the performance of single-protein/gene receiver operating characteristic in differentiating the invasive encapsulated follicular variant of papillary thyroid carcinoma from others within The Cancer Genome Atlas projects, which yielded an AUC >0.5. …”
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Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil by Karan Khadayat, Dawa Dindu Sherpa, Krishna Prakash Malla, Sunil Shrestha, Nabin Rana, Bishnu P. Marasini, Santosh Khanal, Binod Rayamajhee, Bibek Raj Bhattarai, Niranjan Parajuli

“…Here, we have also analyzed 16S rRNA in 27 Streptomyces species whose whole-genome sequence is available, which has revealed that some species have multiple copies of the 16S gene (∼1.5 kb) with significant variation in nucleotides. …”
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