Genetic insights and therapeutic avenues: unraveling the role of polyunsaturated fatty acids as mediators between hypothyroidism and Von Willebrand disease through Mendelian random... by Wenting Zhou, Rui He, Ruwei Ou

“…In this study, we used a Mendelian randomization study to analyze the causal relationship between Hypothyroidism and VWD and to investigate whether polyunsaturated fatty acids mediate the effects of Hypothyroidism on VWD.MethodsUsing a large publicly available genome-wide association study of predominantly European ancestry to obtain data on Hypothyroidism, VWD, and PUFA, we conducted a two-sample Mendelian randomization study to assess the causal relationship between Hypothyroidism and VWD and assess the potential role of Polyunsaturated fatty acids in mediating the causal pathway between Hypothyroidism and VWD. …”
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Developmental and hormonal regulation of Arabidopsis thaliana ornithine-delta-aminotransferase by A. A. Egorova, S. V. Gerasimova, A. V. Kochetov

“…In the present work, the reporter gene construct containing the Escherichia coli β-glucuronidase gene (gus) under control of the A. thaliana OAT gene promoter was introduced into the genome of A. thaliana ecotype Columbia plants using the floral dip method; GUS activity was assayed in different experimental conditions including hormone treatment, low and high nitrogen and salinity. …”
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Chemokine family significance and prognostic value in colorectal cancer by Yi Ding, Yi Ding, Yi Ding, Yinnan Chen, Yinnan Chen, Yinnan Chen, Siyun Xie, Quanpeng Qiu, Quanpeng Qiu, Xiaolong Guo, Yun Feng, Yun Feng, Hongxia Li, Hongxia Li, Fang Zhu, Yaping Liu, Yaping Liu, Yaping Liu

“…This study was rigorously augmented using comprehensive datasets from The Cancer Genome Atlas (TCGA), ensuring a robust analysis of gene expression patterns associated with clinical outcomes.ResultsThrough data analysis, we identified key genes from the chemokine family thought pertinent to CRC outcomes. …”
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Immunoinfiltration Analysis of Mitochondrial Damage‐Related Genes in Lung Adenocarcinoma and Construction of a Classification and Prognostic Model Integrated With WGCNA and Machine... by Jirong Zhang, Lin Lin

“…Materials & Methods Using GeneCards and The Cancer Genome Atlas (TCGA) databases, we screened differentially expressed mitochondrial damage‐related genes in LUAD. …”
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Kruppel-family zinc finger proteins as emerging epigenetic biomarkers in head and neck squamous cell carcinoma by Patrick Pearson, Kendra Smith, Nilita Sood, Elizabeth Chia, Alicia Follett, Michael B. Prystowsky, Simon Kirby, Thomas J. Belbin

“…Methods We examined prognostic significance of ZNF154 and ZNF132 expression and DNA methylation in independent patient cohort of about 500 head and neck cancer patients in the Cancer Genome Atlas (TCGA). We also overexpressed these genes in HEK-293 cells, as well as the oral cancer cell line UM-SCC-1. …”
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Online resources on gene networks containing human and animal data by E. V. Ignatieva, D. A. Afonnikov, N. A.  Kolchanov

“…Gene networks are molecular genetic systems that ensure the formation of phenotypic characteristics of organisms (molecular, biochemical, structural, morphological, behavioral, etc.) based on  information encoded in their genomes. Reconstruction of gene networks provides a methodological basis for modern systems biology. …”
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Multi-generational adaptation to Solanum nigrum increases reproduction and decreases microbial diversity of Aphis gossypii by Peng Wang, Peng Wang, Yu-Xi Jing, Ya-Jie Ma, Ya-Jie Ma, Dan Wang, Yong-Pan Shan, Hongyan Hu, Changcai Wu, Xian-Peng Song, Xiangliang Ren, Xiangliang Ren, Xiangliang Ren, Yan Ma, Yan Ma, Yan Ma, Xiaoyan Ma, Xiaoyan Ma, Xiaoyan Ma

“…KEGG (Kyoto Encyclopedia of Genes and Genomes) function prediction analysis suggested that the roles of symbiotic bacteria in A. gossypii are primarily linked to metabolic processes. …”
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The left amygdala is genetically sexually-dimorphic: multi-omics analysis of structural MRI volumes by Yuanyuan Gui, Geyu Zhou, Shuya Cui, Hongyu Li, Hui Lu, Hongyu Zhao

“…We also constructed polygenic risk scores (PRS) to investigate sex-specific genetic correlations between left amygdala volume and mental disorders (N = 25,576~105,318) of Psychiatric Genomics Consortium and other traits of UKB (N = 347,996). …”
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Loss of protein C vs protein S results in discrepant thrombotic phenotypes by Chia-Jui Ku, Xinge Yu, Queena Y. Zhao, Steven J. Grzegorski, Jeffrey G. Daniel, Allison C. Ferguson, Jordan A. Shavit

“…Here, we generate proc (zebrafish PROC ortholog) and pros1 knockouts through genome editing in zebrafish and uncover partially discordant phenotypes. proc−/− mutants exhibited ∼70% lethality at 1 year of age, whereas pros1−/− survival was unaffected. …”
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Signatures of lower respiratory tract microbiome in children with severe community-acquired pneumonia using shotgun metagenomic sequencing by Ting-Yu Yen, Ching Hsu, Ni-Chung Lee, Chao-Szu Wu, Hsin Wang, Kuan-Yi Lee, Chia-Ray Lin, Chun-Yi Lu, Mo-Li Tsai, Tzu-Yu Liu, Che Lin, Chien-Yu Chen, Luan-Yin Chang, Feipei Lai, Li-Min Huang

“…Signatures of lower respiratory tract microbiome were compared between groups using an exact k-mer matching metagenomic analysis pipeline (Kraken 2) and a metagenome-assembled genomes pipeline (MetaWRAP). Results: Totally 66 children were enrolled, and 27 children were critical cases, and the rest were severe cases. …”
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Prognostic value of FCER1G expression and M2 macrophage infiltration in esophageal squamous cell carcinoma by Wei Peng, Yali Zhao, Ningning Yang, Yan Fang, Yintong Wu, Zhenzhong Feng, Qiang Wu, Xian Wang

“…Methods The expression of FCER1G and its prognostic value in ESCC was examined by The Cancer Genome Atlas and Gene Expression Omnibus databases. …”
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Mitochondrial dysfunction-driven AMPK-p53 axis activation underpins the anti-hepatocellular carcinoma effects of sulfane sulfur by Xue Zheng, Yuhua Luo, Rui Huo, Yiwen Wang, Youbang Chen, Mianrong Chen, Qi Zhao, Kexin Li, Hanyi Zhang, Xiaotong Li, Xiang Li, Hui Zhang, Zaopeng He, Li Huang, Chun-tao Yang

“…We utilized bioinformatics algorithms for clustering, function enrichment, feature screening and survival analysis on proteomic data from the Cancer Proteome Atlas (CPTAC) and transcriptomic data from the Cancer Genome Atlas (TCGA). The impact of PSCP on HCC was assessed in vitro and in vivo, focusing on the expression and activity of p53 and AMP-activated protein kinase (AMPK), as well as mitochondrial function. …”
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Absent in melanoma 2: a potent suppressor of retinal pigment epithelial-mesenchymal transition and experimental proliferative vitreoretinopathy by Yu Chen, Mingyuan Jiang, Liping Li, Shanshan Yang, Zuimeng Liu, Shiwen Lin, Wanxiao Wang, Jinyang Li, Feng Chen, Qiang Hou, Xiaoyin Ma, Ling Hou

“…Kyoto Encyclopedia of Genes and Genome analysis revealed that the PI3K-AKT signaling pathway was significantly related to RPE-EMT and that AIM2 inhibited AKT activation in RPE cells by reducing its phosphorylation. …”
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Genetic causality between insomnia and specific orthopedic conditions: Insights from a two-sample Mendelian randomization study by Mingyi Yang, Jiale Xie, Yani Su, Ke Xu, Pengfei Wen, Xianjie Wan, Hui Yu, Zhi Yang, Lin Liu, Peng Xu

“…Objective: To investigate the genetic causality for the insomnia and common orthopedic diseases, such as rheumatoid arthritis (RA), ankylosing spondylitis (AS), osteoporosis (OP), and gout (GT). Methods: The genome-wide association study (GWAS) summary data on insomnia were obtained from a published study, while the GWAS summary data on RA, AS, OP, and GT were sourced from the FinnGen consortium. …”
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High-Throughput Oxford Nanopore Sequencing Unveils Complex Viral Population in Kansas Wheat: Implications for Sustainable Virus Management by Nar B. Ranabhat, John P. Fellers, Myron A. Bruce, Jessica L. Shoup Rupp

“…A phylogenetic analysis of <i>Emaravirus tritici</i> based on RNA 3A and 3B segments and whole-genome characterization of <i>Furovirus tritici</i> were also conducted. …”
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Transcriptional regulatory network analysis identifies GRN as a key regulator bridging chemotherapy and immunotherapy response in small cell lung cancer by Seungyeul Yoo, Ayushi S. Patel, Sarah Karam, Yi Zhong, Li Wang, Feng Jiang, Ranran Kong, Sharon Bikvan, Wenhui Wang, Abhilasha Sinha, Charles A. Powell, Jun Zhu, Hideo Watanabe

“…In this study, we aimed to gain insights into those leveraging a recent surge in the field of SCLC genomics. We constructed a regulatory network for SCLC and identified granulin precursor (GRN) as a hub of EP response associated genes. …”
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A SYBR green I-based multiplex real-time PCR for simultaneous detection of pseudorabies virus, porcine circovirus 3 and porcine parvovirus by Lihua Cao, Wenke Lv, Anqi Li, Lulu Yang, Feng Zhou, Feng Wen, Sheng Yuan, Shujian Huang, Zhili Li, Jinyue Guo

“…Methods In the present study, we established a multiplex real-time PCR assay based on SYBR Green I for the simultaneous detection of PRV, PPV and PCV3 genomes. Results PRV, PPV and PCV3 were distinguished in the same sample by their different melting temperatures (Tm), with melting peaks at 90 °C for PRV, 84 °C for PPV and 80 °C for PCV3, respectively, and other non-targeted swine pathogens did not exhibit specific melting peaks. …”
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Differential expressed genes (DEGs) and differential alternative splicing genes (DASs) revealed the common pathologic mechanism of three bacterial pathogens to American eels (Angui... by Zihao Chen, Guanghua Sun, Songlin Guo

“…Gene Ontology (GO) enrichment analysis identified 23 common terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis highlighted 18 common pathways. …”
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Potential Virulence and Survival of Acid-Resistant Bacillus cereus KS100Bc Isolated from Foodservice Establishments in Korea by Seung Min Kim, Daekeun Hwang, Hyun Jung Kim

“…The parameter Kmax of the log-linear + tail model for ATCC 14579 was up to1.31-fold higher than that for KS100Bc in SGF, indicating that it is difficult to inactivate KS100Bc in acidic gastric environments. Although whole-genome sequencing identified nucleotide sequences with high homology (>99%) between ATCC 14579 and KS100Bc, significant differences were observed in phenotypes such as biofilm formation and survival in SGF. …”
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Bacteriophages RCF and 1-6bf can control the growth of avian pathogenic Escherichia coli by Farah Khan, Hafsa Naseem, Muhammad Asif, Iqbal Alvi, Shafiq ur Rehman, Abdul Rehman

“…Rcf has an icosahedral capsid of 18 nm with a tail size of 5 nm, while 1-6bf has an elongated head capsid of 93 nm and a short non-contractile tail of 8 nm with tail fibers for attachment. RCF and 1-6bf have genome sizes of 38 kb and 77 kb, with GC content of 50.98 % and 42.1 % respectively. …”
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