Selective suppression of influenza A/H5N1 virus replication <i>in vitro</i> using nanocomplexes consisting of siRNA and aminopropylsilanol nanoparticles by Marina N. Repkova, Asya S. Levina, Oleg Yu. Mazurkov, Elena V. Makarevich, Ekaterina I. Filippova, Natalya A. Mazurkova, Valentina F. Zarytova

“…The most effective nanocomplex that contained siRNA targeted at the chosen region of mRNA segment 5 of the viral genome reduced virus replication in the culture by a factor of 630. …”
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Identification and functional analysis of energy metabolism and pyroptosis-related genes in diabetic nephropathy by Shan He, Jian Ye, Yu Wang, Lu yang Xie, Si Yi Liu, Qin kai Chen

“…Subsequent analyses included Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, Gene Set Enrichment Analysis (GSEA), and Protein-Protein Interaction (PPI) network analysis. …”
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Genotype-driven sensitivity of mice to tick-borne encephalitis virus correlates with differential host responses in peripheral macrophages and brain by Michaela Berankova, Jiri Holoubek, Vaclav Hönig, Zuzana Matusova, Martin Palus, Jiri Salat, Imtissal Krayem, Jarmila Vojtiskova, Pavel Svoboda, Veronika Pranclova, Lukas Valihrach, Peter Demant, Marie Lipoldova, Daniel Ruzek

“…BALB/c mice have intermediate susceptibility to TBE virus (TBEV) and STS mice are highly resistant, whereas the recombinant congenic strain CcS-11, which carries 12.5% of the STS genome on the BALB/c background, is more susceptible than BALB/c mice. …”
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Traces of Paleolithic expansion in the Nivkh gene pool based on data on autosomal SNP and Y chromosome polymorphism by V. N. Kharkov, N. A. Kolesnikov, L. V. Valikhova, A. A. Zarubin, A. L. Sukhomyasova, I. Yu. Khitrinskaya, V. A. Stepanov

“…The gene pool of the Nivkhs and other Far Eastern and Siberian populations was characterized using a genome-wide panel of autosomal single-nucleotide polymorphic markers and Y chromosome haplogroups. …”
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Creation of body color mutants by CRISPR/Cas9 gene editing in largemouth bass, Micropterus salmoides by Lina Sun, Tian Gao, Zhiqiang Li, Xiaolong Yang, Zuliang Qin, Mingxiao Ye, Ying Li, Feilong Wang, Deshou Wang

“…The efficiency of mutation was gRNA and Cas9 mRNA concentration dependent, and 400 ng/μl gRNA and 800 ng/μl Cas9 mRNA were recommended as the appropriate injection dose for genome editing in LMB. Enzyme-digestion and sanger-sequencing demonstrated that the CRISPR/Cas9 system is available for the gene editing of LMB. …”
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How does gut microbiota affect the vaginitis axis? The mediating role of plasma metabolites by Mo Li, Qianyu Zhang, Tong Wu, Lanfang Ma, Dianxing Hu, Zixuan Yuan, Shixuan Wang, Aiyue Luo, Jinjin Zhang

“…Here, we explored whether the gut microbiota influences the risk of vaginitis by performing a two-sample Mendelian randomization analysis using the largest genome-wide association studies to date. Four gut taxa in genus levels were identified related to vaginitis: Candidatus Soleaferrea (inverse-variance weighted [IVW] odds ratio [OR] = 2.20, P = 0.026), Dialister (IVW OR = 2.62, P = 0.029), Lachnospiraceae UCG-008 (IVW OR = 0.41, P = 0.0067), and Ruminiclostridium 5 (IVW OR = 0.080, P = 1.42 × 10−5). …”
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A Network Pharmacology-Based Strategy for Unveiling the Mechanisms of Tripterygium Wilfordii Hook F against Diabetic Kidney Disease by Yuyang Wang, Tongtong Liu, Fang Ma, Xiaoguang Lu, Huimin Mao, Weie Zhou, Liping Yang, Ping Li, Yongli Zhan

“…Finally, all the targets of clusters were subjected for Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses via DAVID. …”
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CD2AP deficiency aggravates Alzheimer’s disease phenotypes and pathology through p38 MAPK activation by Yan-Yan Xue, Zhe-Sheng Zhang, Rong-Rong Lin, Hui-Fen Huang, Ke-Qing Zhu, Dian-Fu Chen, Zhi-Ying Wu, Qing-Qing Tao

“…Abstract Background Alzheimer’s disease (AD) is the most common form of neurodegenerative disorder, which is characterized by a decline in cognitive abilities. Genome-wide association and clinicopathological studies have demonstrated that the CD2-associated protein (CD2AP) gene is one of the most important genetic risk factors for AD. …”
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Polytrauma in Older Adults Leads to Significantly Increased TIMP-1 Levels in the Early Posttraumatic Period by Mareen Braunstein, Thomas Kusmenkov, Wolf Mutschler, Christian Kammerlander, Wolfgang Böcker, Viktoria Bogner-Flatz

“…MMP-9 and TIMP-1 have been found to play a major role in posttraumatic immune disorder in a previous genome-wide mRNA analysis. Objective. The aim of this study was to evaluate the differences in serum protein dynamics in older and younger polytraumatized adults. …”
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Infarct‐like myocarditis with coronary vasculitis and aneurysm formation caused by Epstein–Barr virus infection by Cristina Chimenti, Romina Verardo, Claudia Grande, Marco Francone, Andrea Frustaci

“…Two additional frozen samples were evaluated by real‐time polymerase chain reaction for the presence of cardiotropic viral genomes. Routine laboratory tests revealed the presence of elevated white blood cells (17 000 103/μL) and increased Troponin T. …”
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Transfer of Cytoplasmic Male Sterility to the Female Parents of Heat- and Drought-Resilient Maize (<i>Zea mays</i> L.) Hybrids by Ayyanagouda Patil, Kushal Gowda, Shivananda T. Lakshman, Prakash H. Kuchanur, Gajanan Saykhedkar, Sudha Krishnan Nair, Kisan B. Jadhav, Sharanabasappa Yeri, Gururaj Sunkad, Jayaprakash M. Nidagundi, Vinayan Madhumal Thayil, Pervez H. Zaidi

“…The present research used Diversity Array Technology (DArTag) and Kompetitive Allele Specific PCR (KASP)-based single-nucleotide polymorphic markers for background selection in backcross populations. Genome recovery percentage ranged from 64.25 to 72.70, 78.94 to 87.69 and 82.28 to 90.77 percent in the BC₁F₁, BC₂F₁ and BC₃F₁ population, respectively, in the CAL1514 population, while it was 63.47 to 73.55, 78.16 to 88.76 and 83.96 to 91.81 percent in the BC₁F₁, BC₂F₁ and BC₃F₁ population, respectively, in the ZL153493 population. …”
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Impact of prior SARS-CoV-2 infection and COVID-19 vaccination on the subsequent incidence of COVID-19: a multicentre prospective cohort study among UK healthcare workers – the SIRE... by Nick Andrews, Mary Ramsay, Maria Zambon, Victoria Hall, Sarah Wallace, Susan Hopkins, Jean Timeyin, Tim J G Brooks, Amoolya Vusirikala, Meera Chand, Andre Charlett, Sakib Rokadiya, Colin S Brown, Madhumita Shrotri, Peter D Kirwan, Michele Cole, Natalie Gillson, Ana Atti, Sarah Foulkes, Andrew Taylor-Kerr, Blanche Oguti, Jasmin Islam

“…Secondary outcomes include incidence and prevalence (both RNA and antibody) of SARS-CoV-2, viral genomics, viral culture, symptom history and antibody/neutralising antibody titres.Ethics and dissemination The study was approved by the Berkshire Research Ethics Committee, Health Research Authority (IRAS ID 284460, REC reference 20/SC/0230) on 22 May 2020; the vaccine amendment was approved on 12 January 2021. …”
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Autophagy activation in response to cigarette smoke: Exploring the disparity in laryngeal cancer incidence and outcomes between sexes in South Korea by Min Ji Kim, Jisu Hong, Hyo Won Chang, Yun-Hee Lee, Jun-Pyo Myong, Ah Ra Jung, Yoon Se Lee, Minsu Kwon

“…In addition, to explore the relationship between the level of autophagy-related gene expression and clinical features, female LC patients were compared with male patients through an analysis of data from The Cancer Genome Atlas (TCGA). Results: In the NHIS data analyses, male LC patients had an 11 times higher incidence than female patients, even after adjusting for smoking and age. …”
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Heterogeneity of avian infectious bronchitis virus population by Ye. V. Ovchinnikova, L. O. Scherbakova, S. N. Kolosov, A. N. Andriyasova, N. G. Zinyakov, Z. B. Nikonova, A. A. Kozlov, P. B. Akshalova, D. A. Altunin, D. B. Andreychuk

“…Due to the lack of mechanisms to correct errors during genome replication, the virus can quickly mutate and generate new strains. …”
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Identification of Co-Expression Modules of Cotton Plant Height-Related Genes Based on Weighted Gene Co-Expression Network Analysis by Qian Huang, Li Liu, Hang Li, Xuwen Wang, Aijun Si, Liangrong He, Yu Yu

“…Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that these modules are associated with biologically significant pathways, including oxidoreductase activity, transcriptional regulator activity, catalytic activity, and phosphate hydrolase activity. …”
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Assessment of genetic polymorphism in sea buckthorn (Hippophae L.) of different environmentally-geographical origin by ISSR-markers by A. Y. Zemtsova, Y. A. Zubarev, A. V. Gunin, M. S. Ivanova

“…The absence of an approved procedure of genome definition for Siberian sea buckthorn makes the above task quite complicated. …”
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A Metabolic Gene Signature to Predict Overall Survival in Head and Neck Squamous Cell Carcinoma by Zeng-Hong Wu, Yun Tang, Yue Zhou

“…In this study, we used RNA sequencing (RNA-seq) data from the cancer genome atlas (TCGA), with validation in the GEO dataset to profile the metabolic microenvironment and define potential biomarkers for metabolic therapy. …”
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Integrating RNA-seq and scRNA-seq to explore the prognostic features and immune landscape of exosome-related genes in breast cancer metastasis by Guanyou Huang, Yong Yu, Heng Su, Hongchuan Gan, Liangzhao Chu

“…Objective This study aims to explore the role of exosome-related genes in breast cancer (BRCA) metastasis by integrating RNA-seq and single-cell RNA-seq (scRNA-seq) data from BRCA samples and to develop a reliable prognostic model.Methods Initially, a comprehensive analysis was conducted on exosome-related genes from the BRCA cohort in The Cancer Genome Atlas (TCGA) database. Three prognostic genes (JUP, CAPZA1 and ARVCF) were identified through univariate Cox regression and Lasso-Cox regression analyses, and a metastasis-related risk score model was established based on these genes. …”
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The heterogeneity of NOTCH1 to tumor immune infiltration in pan-cancer by XiaoJun Duan, Rihan Wu, Mingyang Zhang, Kexin Li, Lei Yu, Huirong Sun, Xingxia Hao, Changshan Wang

“…In this study, the data originated from the Genotype-Tissue Expression (GTEx) and the Cancer Genome Atlas (TCGA) databases were input into multiple online bioinformatic tools to study the characteristics of NOTCH1 in pan-cancer. …”
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Deficiency of the palmitoyl acyltransferase ZDHHC7 modulates depression-like behaviour in female mice after a mild chronic stress paradigm by Christa Hohoff, Nicole Kerkenberg, Mingyue Zhang, Weronika Palkowska, Lydia Wachsmuth, Maja Peng, Lena Stiehl, Christiane Schettler, Johannes C. S. Zang, Andreas Huge, Evgeni Ponimaskin, Cornelius Faber, Bernhard T. Baune, Weiqi Zhang

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