The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels by Andrey A. Ayginin, Ekaterina V. Pimkina, Alina D. Matsvay, Anna S. Speranskaya, Marina V. Safonova, Ekaterina A. Blinova, Ilya V. Artyushin, Vladimir G. Dedkov, German A. Shipulin, Kamil Khafizov

“…However, unlike the metagenomic studies using 16S rRNA for the detection of bacteria, it is impossible to create universal oligonucleotides to target all known and novel viruses, owing to their genomic diversity and variability. On the other hand, sequencing the entire genome is still expensive and has relatively low sensitivity for such applications. …”
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Generation of iPSCs by Nonintegrative RNA-Based Reprogramming Techniques: Benefits of Self-Replicating RNA versus Synthetic mRNA by Heidrun Steinle, Marbod Weber, Andreas Behring, Ulrike Mau-Holzmann, Christian Schlensak, Hans Peter Wendel, Meltem Avci-Adali

“…Using both RNA-based methods, integration-free iPSCs without genomic alterations were obtained. The pluripotency characteristics identified by specific marker detection and the in vitro and in vivo trilineage differentiation capacity were comparable. …”
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In Vitro Analyses Reveal the Effect of Synthetic Cytokinin Forchlorfenuron (FCF) on a Septin-Like Protein of Taeniid Cysticerci by Diana G. Rios-Valencia, Edgar O. López-Villegas, Dylan Diaz Chiguer, Adrian Marquez Navarro, Ruben D. Díaz-Martín, Benjamin Nogueda-Torres, Javier R. Ambrosio

“…Cytokinin forchlorfenuron (FCF), a synthetic cytokinin, has been used specifically for the characterization of septins. In spite of genomic evidence of their existence, nothing is known about septin filaments in taeniid cestodes. …”
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Deficiency of Dietary Fiber in Slc5a8-Null Mice Promotes Bacterial Dysbiosis and Alters Colonic Epithelial Transcriptome towards Proinflammatory Milieu by Sathish Sivaprakasam, Pramodh K. Ganapathy, Mohd Omar Faruk Sikder, Moamen Elmassry, Sabarish Ramachandran, Kameswara Rao Kottapalli, Vadivel Ganapathy

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Orbital Desmoid-Type Fibromatosis: A Case Report and Literature Review by Alessandro Moro, Paolo De Angelis, Giulio Gasparini, Sandro Pelo, Gianluigi Petrone, Emanuela Lucci Cordisco, Umberto Garagiola, Giuseppe D’Amato, Gianmarco Saponaro

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Molecular Detection of Panton Valentine Leukocidin Toxin in Clinical Isolates of Staphylococcus aureus from Kiambu County, Kenya by Sani Iliya, Jonathan Mwangi, Ronald Maathai, Mary Muriuki, Christopher Wainaina

“…The detection of PVL toxins was performed by amplification of genomic DNA and by reverse hybridization that identifies PVL genes using Geno Type MRSA kit. …”
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Direct Reprogramming of Mouse Fibroblasts to Neural Stem Cells by Small Molecules by Yan-Chuang Han, Yoon Lim, Michael D. Duffieldl, Hua Li, Jia Liu, Nimshitha Pavathuparambil Abdul Manaph, Miao Yang, Damien J. Keating, Xin-Fu Zhou

“…The risk hurdles are associated with viral transduction vectors induced mutagenesis, tumor formation from undifferentiated stem cells, and transcription factors-induced genomic instability. Here we describe a viral vector-free and more efficient method to induce mouse fibroblasts into NSC using small molecules. …”
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Evaluation of Antimicrobial Activity of Four Organic Acids Used in Chicks Feed to Control Salmonella typhimurium: Suggestion of Amendment in the Search Standard by Aicha El Baaboua, Mohamed El Maadoudi, Abdelhakim Bouyahya, Omar Belmehdi, Ayoub Kounnoun, Rajae Zahli, Jamal Abrini

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Integrating phylogeographic and phenotypic evidence to delimit deep evolutionary lineages in the Dryophytes japonicus species complex, with an assessment of their conservation need... by Amaël Borzée, Yikweon Jang, Siti N. Othman, Jordy Groffen, Irina V. Maslova, Zoljargal Purevdorj, Kiyomi Yasumiba, Tomohiko Shimada, Yoonjung Yi, Natalya Alekseevna Schepina, Desiree Andersen, Yoonhyuk Bae, Ikuo Miura, Christophe Dufresnes

“…In this study, we assessed the genetic, genomic, morphological, and acoustic diversity and differentiation within the D. japonicus complex to propose taxonomic arrangements and assess taxon-specific threats for each defined clade. …”
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The case for development of a core outcome set (COS) and supplemental reporting guidelines for influenza vaccine challenge trial research in swine by Sheila Keay, Famke Alberts, Annette M. O’Connor, Robert Friendship, Terri O’Sullivan, Zvonimir Poljak

“…The checklist serves two purposes: as a reminder of details essential to report for inclusion of findings in meta-analyses and sub-group meta-analyses (e.g., antigenic or genomic descriptions of influenza vaccine and challenge viruses), and as an aid to help synthesis researchers fully characterize and comprehensively include studies in reviews.…”
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Efficient chimeric mouse production using a novel embryonic stem cell line by G. V. Kontsevaya, N. A. Feofanova, A. G. Menzorov, I. E. Pristyazhnyuk, A. V. Smirnov, N. R. Battulin, L. A. Gerlinskaya

“…Never-theless, there is a need for complex genome modifications, such as large deletions, reporter genes insertion into the 3’ gene regulatory sequence, or site-specific modifications of the genome. …”
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TRα1 mutant suppresses KLF9 to cause endometrial metaplasia with ectopic IL-33 expression leading to uterine fibrosis and infertility by Elijah Edmondson, Takahito Kimura, Eunmi Hwang, Minjun Kim, Andrew Warner, Yuelin Zhu, Li Zhao, Yanlin Yu, Xuguang Zhu, Maria Hernandez, Noemi Kedei, Sheue-yann Cheng

“…Abstract Thyroid hormone receptors (TRs) mediate the genomic actions of thyroid hormone. Mutations of THRA gene cause a human disease known as resistance to thyroid hormone (RTHα). …”
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Genetics polymorphism of poplars from Moscow region based on high-throughput sequencing of ITS by E. V. Borkhert, G. S. Krasnov, N. L. Bolsheva, Р. Kezimana, O. Yu. Yurkevich, O. V. Muravenko, A. V. Kudryavtseva, N. V. Melnikova

“…Bioinformatics analysis was performed using CLC Genomics Workbench tool. The involved set of poplars had a high level of genetic diversity – the number of single nucleotide polymorphisms (SNPs) detected in each genotype relative to the reference ITS1 and ITS2 sequences of P. trichocarpa varying from 4 to 44. …”
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Protein C deficiency with recurrent systemic thrombosis associated with compound heterozygous PROC missense variants by Mikio Shiba, Shuichiro Higo, Yu Morishita, Yasuhiro Ichibori, Yoshihiro Kin, Yasushi Sakata, Yoshiharu Higuchi

“…PCR amplification of genomic DNA spanning these exons followed by Sanger sequencing analysis revealed that the c.76G>A and the synonymous c.423G>T variants were in the same allele, whereas the c.1000G>A variant was on the opposite allele, indicating compound heterozygosity. …”
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Avirulence depletion assay: Combining R gene-mediated selection with bulk sequencing for rapid avirulence gene identification in wheat powdery mildew. by Lukas Kunz, Jigisha Jigisha, Fabrizio Menardo, Alexandros G Sotiropoulos, Helen Zbinden, Shenghao Zou, Dingzhong Tang, Ralph Hückelhoven, Beat Keller, Marion C Müller

“…In a proof-of-concept experiment we mapped the AVR component of the wheat immune receptor Pm3a to a 25 kb genomic interval in Bgt harboring a single effector, the previously described AvrPm3a2/f2. …”
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Different DNA Loidy Patterns for the Differentiation of Common Subtypes of Renal Tumors by Guorong Li, Michèle Cottier, Odile Sabido, Anne Gentil-Perret, Claude Lambert, Christian Genin, Jacques Tostain

“…DNA ploidy is viewed as a marker of gross genomic aberrations. The aim of this study is to evaluate the DNA ploidy in the common subtypes of renal tumors to increase our understanding of renal tumor biology and to broaden clinical application of DNA ploidy. …”
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Smart breeding driven by advances in sequencing technology by Chenji Zhang, Sirong Jiang, Yangyang Tian, Xiaorui Dong, Jianjia Xiao, Yanjie Lu, Tiyun Liang, Hongmei Zhou, Dabin Xu, Han Zhang, Ming Luo, Zhiqiang Xia

“…This review provides an overview of breeding techniques from traditional selective breeding and crossbreeding to modern breeding based on molecular marker‐assisted and genomic selection. Systematic analysis of the mainstream genotyping approaches based on principle, application scenario and supporting software revealed that the changes in genotyping technology have led to an explosion in data. …”
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All Roads Lead to Rome: Pathways to Engineering Disease Resistance in Plants by Aziz Ul Ikram, Muhammad Saad Shoaib Khan, Faisal Islam, Sulaiman Ahmed, Tengfang Ling, Feng Feng, Zongtao Sun, Huan Chen, Jian Chen

“…Recent developments in genetic engineering, including efficient gene manipulation and transformation methods, gene editing and synthetic biology, coupled with the understanding of microbial pathogenicity and plant immunity, both at molecular and genomic levels, have enhanced the capabilities to develop disease resistance in plants. …”
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Analysis of Bacterial Diversity in Different Heavy Oil Wells of a Reservoir in South Oman with Alkaline pH by Biji Shibulal, Saif N. Al-Bahry, Yahya M. Al-Wahaibi, Abdulkadir E. Elshafie, Ali S. Al-Bemani, Sanket J. Joshi

“…Molecular approaches like proteomic and genomic characterization of the isolates are replacing the traditional method of identification with systemic classification. …”
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Actively protective combinatorial analysis: A scalable novel method for detecting variants that contribute to reduced disease prevalence in high-risk individuals by J Sardell, S Das, K Taylor, C Stubberfield, A Malinowski, M Strivens, S Gardner

“…We show how this can be used to identify mechanisms in the background of normal cellular biology that work to slow or stop progression of complex, chronic diseases.Actively protective combinatorial analysis identifies combinations of features that contribute to reducing risk of disease in individuals who remain healthy even though their genomic profile suggests that they have high risk of developing disease. …”
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