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2681
Characterization of Microbiota in Endometrial Fluid and Vaginal Secretions in Infertile Women with Repeated Implantation Failure
Published 2019-01-01“…On days 6-8 in the luteal phase of the natural, oocyte-pickup, or hormone replacement cycle, the paired EF and VS samples were obtained separately. Extracted genomic DNA was pyrosequenced for the V4 region of 16S ribosomal RNA using a next-generation sequencer. …”
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2682
Computational biology and artificial intelligence in mRNA vaccine design for cancer immunotherapy
Published 2025-01-01“…This paper reviews technologies that streamline mRNA vaccine development, from genomic sequencing to lipid nanoparticle (LNP) formulation. …”
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2683
Directions for improving the legal regulation of forensic DNA analysis in Ukraine in the context of integration into the European Union
Published 2022-06-01“…Together with the adoption of the Law of Ukraine “On the State Registration of Human Genomic Information”, it is necessary to introduce changes and additions to the Code of Criminal Procedure of Ukraine in order to harmonize the provisions of these normative legal acts, which primarily concern the improvement of the procedure for obtaining samples for molecular genetic research. …”
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2684
Immune-Boosting and Antiviral Effects of Antioxidants in COVID-19 Pneumonia: A Therapeutic Perspective
Published 2025-01-01“…Furthermore, these molecules may mitigate the symptoms of COVID-19 by exerting direct antiviral effects that inhibit the infection process and genomic replication of SARS-CoV-2 in host cells. Moreover, antioxidants may work synergistically with standard antiviral treatments to reduce viral-induced oxidative damage. …”
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2685
Prevalence of Ticks Infesting Dairy Cattle and the Pathogens They Harbour in Smallholder Farms in Peri-Urban Areas of Nairobi, Kenya
Published 2021-01-01“…Each animal was examined for attached ticks followed by morphological tick identification at the species level. Genomic DNA was extracted from each of the ticks, and 16S rDNA gene was amplified for pathogen identification. …”
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2686
Loss of BRCA1 Spontaneously Induces the Tumorigenesis in Lacrimal Gland
Published 2018-01-01“…These results suggest that BRCA1 is involved in genetic stability of the lacrimal gland, providing new insight into genomic instability in organism maintenance and tumorigenesis of the lacrimal gland.…”
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2687
The genus Entomophthora: bringing the insect destroyers into the twenty-first century
Published 2021-11-01“…However, we are only starting to understand the functions of unusual molecular and genomic characteristics, such as having large > 1 Gb genomes full of repetitive elements and potential functional diploidy. …”
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2688
Genetic diversity assessed in Ethiopian highland bamboo [Oldeania alpina (K. Schum) Stapleton] populations revealed by microsatellite markers
Published 2024-02-01“…Methods The study relied on 150 DNA samples representing 15 O.alpina populations collected across major O.alpina harboring forests of Ethiopia. Following total genomic DNA isolation SSR primer screening was conducted using PCR, gel electrophoreses, gel doc imaging, allele scoring, and statistical analysis. …”
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2689
The problem of conservation of gene pools of domesticated animals
Published 2017-07-01“…Using various DNA markers, methods of genomic and genetic breeding in nature conservation and genetic selection of domesticated species provides an opportunity to obtain data on the genetic potential (value, originality) of the breed, which is important for scientific justification of its preservation.…”
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2690
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2691
Mapping dynamic regulation of gene expression using single-cell transcriptomics and application to complex disease genetics
Published 2025-04-01“…As an application, we show that the approach detects known and new genes associated with schizophrenia and enables insights into context-dependent disease mechanisms. We provide a genomic resource from a phenome-wide application of our models to more than 1,500 phenotypes from the UK Biobank. …”
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2692
The widely used Ucp1-Cre transgene elicits complex developmental and metabolic phenotypes
Published 2025-01-01“…Mapping the transgene insertion site reveals insertion in chromosome 1 accompanied by large genomic alterations disrupting several genes expressed in a range of tissues. …”
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2693
Tracking des transferts des bactéries porteuses de résistances entre animal, homme et environnement
Published 2023-08-01“…Various targeted or pan-genomic molecular biology techniques can be used to better characterise the dissemination of bacterial clones and to identify exchanges of genes and mobile genetic elements between ecosystems.…”
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2694
Occurrence of <i>Pestalotiopsis</i> sp. on Ornamental Plants <i>Camellia japonica</i> L. in Romanian Public Gardens
Published 2025-01-01“…<i>Pestalotiopsis</i> species were identified based on morphological characteristics and Sanger sequencing. Genomic analysis of the fungal isolate coded P_CJ_24, obtained from living plants, was based on ribosomal internal transcribed spacer region amplification using the LSU primers. …”
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2695
Analysis of genetic diversity in sesame (Sesamum indicum L.) germplasm lines based on agro-morphological traits and SSR markers
Published 2024-12-01“…An investigation was carried out with a set of fifty sesame genotypes to study the degree of genetic diversity based on morphological and marker based genotypic data. The genomic isolation was carried out using CTAB method and the molecular diversity was estimated with the help of fifty SSR markers using per cent polymorphism, PIC content and represented in dendogram. …”
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2696
Therapeutic Targeting of Fibroblast Growth Factor Receptors in Gastric Cancer
Published 2015-01-01“…Fibroblast growth factor receptors (FGFR) play key roles in tumor growth via activated signaling pathways in GC. Genomic amplification of FGFR2 leads to the aberrant activation found in GC tumors and is related to survival in patients with GC. …”
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2697
GWAS of a soybean breeding collection from South East and South Kazakhstan for resistance to fungal diseases
Published 2018-08-01“…One of the main problems of soybean production is fungal diseases causing yields losses of up to 30 %. Modern genomic studies can be applied to facilitate efficient breeding research for improvement of soybean fungal disease tolerance. …”
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2698
Diversity of <i>mariner</i>-like elements in Orthoptera
Published 2020-01-01“…In the present study, we explore the diversity of MLEs elements in 16 species of Orthoptera belonging to three infraorders, Acridoidea (Caelifera), Grylloidea (Ensifera), and Tettigoniidea (Ensifera) by combining data mined from computational analysis of sequenced degenerative PCR MLE amplicons and available Orthoptera genomic scaffolds. In total, 75 MLE lineages (Ortmar) were identified in all the studied genomes. …”
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2699
Mangrove plants using deoxyribonucleic acid barcodes for enhancing biodiversity and conservation
Published 2024-07-01“…METHODS: This study focused on selecting the main regions where mangroves are predominantly distributed in the provinces of North Sumatra and Aceh: Percut Sei Tuan and Deli Serdang mangrove areas, Pulau Sembilan and Lubuk Kertang of Langkat mangrove areas in North Sumatra, and Langsa mangrove areas in Aceh. The genomic deoxyribonucleic acid of mangrove plants was isolated from fresh leaf material using the Geneaid genomic deoxyribonucleic acid mini kit. …”
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2700
Methicillin-Resistant <i>Staphylococcus aureus</i>: The Shifting Landscape in the United Arab Emirates
Published 2025-01-01“…Methods: To investigate this influence, genomic profiling of 310 MRSA clinical isolates collected between February and November 2022 was performed using a DNA microarray-based assay. …”
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