Comparison of the evolutionary patterns of DNA repeats in ancient and young invertebrate species flocks of Lake Baikal by Wang Yuxiang, T. E. Peretolchina, E. V. Romanova, D. Y. Sherbakov

“…DNA repeat composition of low coverage (0.1–0.5) genomic libraries of four amphipods species endemic to Lake Baikal (East Siberia) and four endemic gastropod species of the fam. …”
Get full text

Isolation and Molecular Identification of Lactic Acid Bacteria Using 16s rRNA Genes from Fermented Teff (Eragrostis tef (Zucc.)) Dough by Belay Tilahun, Anteneh Tesfaye, Diriba Muleta, Andualem Bahiru, Zewdu Terefework, Gary Wessel

“…A total of 24 purified bacterial isolates were found to be Gram-positive (they are coccus and rod under microscope) and were good acid producers. Genomic DNA of bacterial isolates were extracted using Invisorb® Spin DNA Extraction kit. 16S rRNA of bacterial isolates were amplified using the bacteria universal primers (rD1 and fD1). …”
Get full text

How do we measure the costs, benefits, and harms of sharing data from biomedical studies? A protocol for a scoping review [version 2; peer review: 2 approved] by Lauren Maxwell, Ankur Krishnan, Priya Shreedhar

“…Introduction The benefits of sharing participant-level data, including clinical or epidemiological data, genomic data, high-dimensional imaging data, or human-derived samples, from biomedical studies have been widely touted and may be taken for granted. …”
Get full text

Azurin a potent anticancer and antimicrobial agent isolated from a novel Pseudomonas aeruginosa strain by Nourhan A. Zaghloul, Mona K. Gouda, Yasser Elbahloul, Nancy M. El Halfawy

“…The presence of the azurin gene was confirmed in the genomic DNA using specific oligonucleotide primers, and azurin expression was also detected in the synthesized cDNA, which revealed that the azurin expression is active. …”
Get full text

EFFECT OF FLANKING SEQUENCES ON THE ACCURACY OF THE RECOGNITION OF TRANSCRIPTION FACTOR BINDING SITES by T. M. Khlebodarova, D. Yu. Oshchepkov, V. G. Levitsky, O. A. Podkolodnaya, E. V. Ignatieva, E. A. Ananko, I. L. Stepanenko, N. A. Kolchanov

“…The detected features of in vitro selected TF binding sites impose constraints on their use in the development of computer-assisted methods of the recognition of candidate sites in genomic sequences.…”
Get full text

Variability of morphological features and nuclear DNA content in haploids and doubled haploids of androgenic callus lines of rice (<I>Oryza sativa</I> L.) by M. V. Ilyushko, M. V. Skaptsov, M. V. Romashova

“…The theoretical possibility of the appearance of intraspecific variability among plants with a small number of chromosomes is considered. A scheme of genomic reorganization is proposed for such species: initial plant (2n) → aneuhaploid plants (n + 1) → megasporogenesis and microsporogenesis of the 0-n type, formation of fertile pollen (n + 1) → diploid plant (2n + 2). …”
Get full text

Emerging Norovirus GII.12 infection in 2010 in Northern Taiwan by Chi-Neu Tsai, Yu-Chung Chang, Hsun-Ching Chao, Yi-Hsiang Hsu, Yuan-Hung Wang, Shih-Yen Chen

“…Phylogenetic analysis showed that our GII.12 sequences were closely aligned with reference genomes identified in the United Kingdom and the United States of America. …”
Get full text

Genetics of Endometrial Cancers by Tsuyoshi Okuda, Akihiko Sekizawa, Yuditiya Purwosunu, Masaaki Nagatsuka, Miki Morioka, Masaki Hayashi, Takashi Okai

“…Telomere shortening is another important type of genomic instability observed in endometrial cancer. …”
Get full text

Estimating the Genetic Diversity of Oranges Citrus spp. in South Sulawesi, Indonesia, Using RAPD Markers by Mustika Tuwo, Tutik Kuswinanti, Andi Nasruddin, Elis Tambaru

“…Leaf samples of 13 orange varieties were collected from the five production centers: Pangkep, Sidrap, Bantaeng, North Luwu, and Selayar in South Sulawesi, Indonesia. Genomic DNA extraction from the orange leaves followed the protocol of the DNA Mini Kit Geneaid. …”
Get full text

Novel MFSD8 Variants in a Chinese Family with Nonsyndromic Macular Dystrophy by Qin Xiang, Yanna Cao, Hongbo Xu, Zhijian Yang, Liang Tang, Ju Xiang, Jianming Li, Hao Deng, Lamei Yuan

“…Ophthalmic examinations were performed, and genomic DNA was extracted from available family members. …”
Get full text

The Single-Nucleotide Polymorphism (SNP) Validity to Detect Omicron Variants by Lia Gardenia Partakusuma, Luhung Budiailmiawan, null Budiman, Ida Parwati, null Aryati, Basti Andriyoko, Louisa Markus, Corine Niswara, Cut Nur Cinthia Alamanda

“…Variants of SARS-CoV-2 are determined by the whole genome sequencing (WGS) assay, which is time-consuming, with limited availability (only in several laboratories). …”
Get full text

Exploring potentially synthetic genes related to diarrhetic shellfish toxins production in Prorocentrum sp. via comparative transcriptomics by Danlin Zheng, Jingyi Cen, Peiliang Chen, Ligong Zou, Jian Zou, Qun Li, Songhui Lu

“…While not completely uncovering the biosynthetic pathway of DSTs, our study offers crucial insights and genomic resources for future research on dinoflagellate toxin production mechanisms.…”
Get full text

The mutational landscape of Staphylococcus aureus during colonisation by Francesc Coll, Beth Blane, Katherine L. Bellis, Marta Matuszewska, Toska Wonfor, Dorota Jamrozy, Michelle S. Toleman, Joan A. Geoghegan, Julian Parkhill, Ruth C. Massey, Sharon J. Peacock, Ewan M. Harrison

“…Survival and persistence during colonisation are likely major drivers of S. aureus evolution. Here we applied a genome-wide mutation enrichment approach to a genomic dataset of 3060 S. aureus colonization isolates from 791 individuals. …”
Get full text

Pathogen Detection and Resistome Analysis in Healthy Shelter Dogs Using Whole Metagenome Sequencing by Smriti Shringi, Devendra H. Shah, Kimberly Carney, Ashutosh Verma

“…Fecal samples from 58 healthy shelter dogs across 10 shelters in Kentucky, Tennessee, and Virginia were analyzed using WMS. Genomic DNA was extracted, and bioinformatics analyses were performed to identify pathogens and ARGs. …”
Get full text

The future of plant lectinology: Advanced technologies and computational tools by Vinicius J.S. Osterne, Kyria S. Nascimento, Benildo S. Cavada, Els J.M. Van Damme

“…Phage display libraries enable the identification of carbohydrate-mimetic peptides and the development of ligands for lectins by presenting diverse peptide libraries on bacteriophages. Genomic and transcriptomic analyses facilitate the exploration of the lectome in various plant species by scanning entire datasets to identify genes that contain lectin motifs—specific conserved amino acid sequences involved in carbohydrate recognition—and lectin domains, the larger structural regions that facilitate and stabilize these interactions. …”
Get full text

Comprehensive analysis reveals the tumor suppressor role of macrophage signature gene FCER1G in hepatocellular carcinoma by Deyu Kong, Yiping Zhang, Linxin Jiang, Nana Long, Chengcheng Wang, Min Qiu

“…This study utilized single-cell RNA sequencing and genomic analysis to explore the characteristic genes of macrophages in HCC and their impact on patient prognosis. …”
Get full text

Polymorphism of RAPD and ISSR markers in grain amaranth species by S. V. Lymanska, L. A. Miroshnichenko, T. I. Goptsiy, O. S. Korneeva

“…Due to the lack of research and depending on the environmental conditions, the morphological markers of amaranth are not able to provide sufficient genomic information to the breeder; thus it is necessary to search for reliable genetic markers that allow the genetic diversity of the Amaranthus L. species to be studied and effectively maintained. …”
Get full text

Paternal metabolic and cardiovascular programming of their offspring: A systematic scoping review. by Claudia Eberle, Michaela F Kirchner, Raphaela Herden, Stefanie Stichling

“…<h4>Background</h4>There is lots of evidence that maternal peri-gestational metabolic, genomic and environmental conditions are closely linked to metabolic and cardiovascular outcomes in their offspring later in life. …”
Get full text

Using technology to increase reach and optimize consent experience for a large-scale research program by Emma Coen, Daniel P. Judge, Samantha Norman, John T. Clark, Andrew Cates, Randolph Thornhill, Kelly Hunt, Lori McMahon, Leslie Lenert, Caitlin G. Allen

“…This pragmatic study aimed to improve the consent experience and evaluate its impact on participant decision making, study knowledge, and satisfaction with the In Our DNA SC program, a population-based genomic screening initiative. We compared two consent procedures: standard consent (SC) involving a PDF document and enhanced consent (EC) incorporating a pictograph and true or false questions. …”
Get full text

Multi-locus CRISPRi targeting with a single truncated guide RNA by Molly M. Moore, Siddarth Wekhande, Robbyn Issner, Alejandro Collins, Anna J. Cruz, Yanjing V. Liu, Nauman Javed, Salvador Casaní-Galdón, Jason D. Buenrostro, Charles B. Epstein, Eugenio Mattei, John G. Doench, Bradley E. Bernstein, Noam Shoresh, Fadi J. Najm

“…Abstract A critical goal in functional genomics is evaluating which non-coding elements contribute to gene expression, cellular function, and disease. …”
Get full text