Engineering synthetic signaling receptors to enable erythropoietin-free erythropoiesis by Aadit P. Shah, Kiran R. Majeti, Freja K. Ekman, Sridhar Selvaraj, Devesh Sharma, Roshani Sinha, Eric Soupene, Prathamesh Chati, Sofia E. Luna, Carsten T. Charlesworth, Travis McCreary, Benjamin J. Lesch, Tammy Tran, Simon N. Chu, Matthew H. Porteus, M. Kyle Cromer

“…To address this challenge, we develop highly optimized small molecule-inducible synthetic EPO receptors (synEPORs) using design-build-test cycles and genome editing. By integrating synEPOR at the endogenous EPOR locus in O-negative induced pluripotent stem cells, we achieve equivalent erythroid differentiation, transcriptomic changes, and hemoglobin production using small molecules compared to EPO-supplemented cultures. …”
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Allelic differences in the key genes of betalain biosynthesis in table beet accessions with contrasting root color from the VIR collection by A. S. Mikhailova, D. V. Sokolova, N. A. Shvachko, V. S. Popov, E. K. Khlestkina

“…It is possible to identify the best table beet accessions for further genome editing among a previously selected high-betanin group. …”
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Shared rare genetic variants in multiplex autism families suggest a social memory gene under selection by Kang Seon Lee, Taeyeop Lee, Mujun Kim, Elizaveta Ignatova, Hyo-Jeong Ban, Min Kyung Sung, Younghoon Kim, Youn-Jae Kim, Jin-Hee Han, Jung Kyoon Choi

“…The results of our population genetic and evolutionary analyses, behavior experiments, and genome editing propose a molecular mechanism that may confer a selective advantage through social memory enhancement and may cause autism-related social impairment when disrupted in humans. …”
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Loss of protein C vs protein S results in discrepant thrombotic phenotypes by Chia-Jui Ku, Xinge Yu, Queena Y. Zhao, Steven J. Grzegorski, Jeffrey G. Daniel, Allison C. Ferguson, Jordan A. Shavit

“…Here, we generate proc (zebrafish PROC ortholog) and pros1 knockouts through genome editing in zebrafish and uncover partially discordant phenotypes. proc−/− mutants exhibited ∼70% lethality at 1 year of age, whereas pros1−/− survival was unaffected. …”
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Resistance to viruses of potato: current status and prospects by S. S. Makarova, V. V. Makarov, M. E. Taliansky, N. O. Kalinina

“…Recently developed new genome editing technologies with the potential to be a powerful tool for gene design open up broad opportunities for development of next-generation resistance genes. …”
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Alkaloids of narrow-leaved lupine as a factor determining alternative ways of the crop’s utilization and breeding by M. A. Vishnyakova, A. V. Kushnareva, T. V. Shelenga, G. P. Egorova

“…Information is briefly presented about the genomic resources of the species and the prospects for their use in marker-assistant selection and genome editing.…”
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Transitioning from wet lab to artificial intelligence: a systematic review of AI predictors in CRISPR by Ahtisham Fazeel Abbasi, Muhammad Nabeel Asim, Andreas Dengel

“…CRISPR-Cas9 based genome editing is a multi-step process such as designing a precise gRNA, selecting the appropriate Cas protein, and thoroughly evaluating both on-target and off-target activity of the Cas9-gRNA complex. …”
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Characterization of Mice Ubiquitously Overexpressing Human 15-Lipoxygenase-1: Effect of Diabetes on Peripheral Neuropathy and Treatment with Menhaden Oil by Lawrence Coppey, Alexander Obrosov, Hanna Shevalye, Eric Davidson, William Paradee, Mark A. Yorek

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THE MORPHOGENIC POTENTIAL OF SIBERIAN POTATO CULTIVARS IN TISSUE CULTURES by S. M. Ibragimova, A. V. Romanova, G. Kh. Myzgina, A. V. Kochetov

“…Recent advances in plant  biotechnology have made it possible to produce resistant cultivars by plant genome editing. This approach allows crop modiications that would be diicult to obtain by conventional breeding techniques. …”
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Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavage by Danyuan Li, Shouyue Zhang, Shuo Lin, Wenjing Xing, Yun Yang, Fengxia Zhu, Dingding Su, Chunlai Chen, Jun-Jie Gogo Liu

“…Two known Cas12e nucleases, DpbCas12e and PlmCas12e, are both effective in genome editing. However, many differences exist in their in vitro dsDNA cleavage activities, reflecting the diversity in Cas12e’s enzymatic properties. …”
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Exploration of the Role of Cyclophilins in Established Hepatitis B and C Infections by Jennifer Molle, Sarah Duponchel, Jennifer Rieusset, Michel Ovize, Alexander V. Ivanov, Fabien Zoulim, Birke Bartosch

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Long-term correction of hemophilia A via integration of a functionally enhanced FVIII gene into the AAVS1 locus by nickase in patient-derived iPSCs by Do-Hun Kim, Sang-Hwi Choi, Jin Jea Sung, Sieun Kim, Hanui Yi, Sanghyun Park, Chan Wook Park, Young Woo Oh, Jungil Lee, Dae-Sung Kim, Jong-Hoon Kim, Chul-Yong Park, Dong-Wook Kim

“…Abstract Hemophilia A (HA) is caused by mutations in coagulation factor VIII (FVIII). Genome editing in conjunction with patient-derived induced pluripotent stem cells (iPSCs) is a promising cell therapy strategy, as it replaces dysfunctional proteins resulting from genetic mutations with normal proteins. …”
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Comprehensive phenotypic analysis of multiple gene deletions of α-glucan synthase and Crh-transglycosylase gene families in Aspergillus niger highlighting the versatility of the fu... by Katharina J. Ost, Mark Arentshorst, Bruno M. Moerschbacher, Mareike E. Dirks-Hofmeister, Arthur F.J. Ram

“…To determine whether the ags and crh gene families have redundant functions, both gene families were deleted using iterative CRISPR/Cas9 mediated genome editing. The 12-fold deletion mutant was viable and did not exhibit growth defects under non-stressing growth conditions. …”
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Altered O-Glycans in stimulated whole saliva from patients with primary Sjögren’s syndrome and non-pSS sicca by Sarah Kamounah, Kristina A. Thomsson, Christiane Elisabeth Sørensen, Eric Paul Bennett, Niclas G. Karlsson, Anne Marie Lynge Pedersen

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Delivery of Prime editing in human stem cells using pseudoviral NanoScribes particles by Thibaut Halegua, Valérie Risson, Julien Carras, Martin Rouyer, Laurent Coudert, Arnaud Jacquier, Laurent Schaeffer, Théophile Ohlmann, Philippe Emmanuel Mangeot

“…Nanoscribes is an asset for development of next generation genome editing approaches using VLPs.…”
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Advances in Molecular Genetics of Reproductive Isolation in Rice by Shunmei LI, Zihan ZHENG, Ruiying LIU, Hongze GUO, Lin ZHENG, Yongyao XIE

“…According to these findings, several strategies and methods such as developing wide compatibility lines, breeding intersubspecific introgression rice and establishing artificial hybrid compatible lines by genome editing have been proposed to overcome RI in rice. …”
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Production of β-lactoglobulin (BLG) gene knock- out blastocyst stage embryos of Indian water buffalo using CRISPR and SCNT technology by Aseem Tara, Priyanka Singh, Devika Gautam, Gaurav Tripathi, Shreya Malhotra, Sacchinandan De, Manoj K. Singh, Naresh L. Selokar

“…With the development of biotechnology, especially genome editing tools (CRISPRs), it is possible to generate new value-added products such as designer hypoallergenic milk for human health benefits. …”
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Advancing date palm cultivation in the Arabian Peninsula and beyond: Addressing stress tolerance, genetic diversity, and sustainable practices by Soumya Koippully Manikandan, Dharshini Jenifer. A, Nisarga K. Gowda, Vaishakh Nair, Rami Al-Ruzouq, Mohamed Barakat A. Gibril, Fouad Lamghari, John Klironomos, Maryam Al Hmoudi, Mohamed Sheteiwy, Ali El-Keblawy

“…Biotechnological advancements, such as genetic transformation and genome editing, are discussed for their potential to enhance crop resilience and productivity. …”
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MaveDB 2024: a curated community database with over seven million variant effects from multiplexed functional assays by Alan F. Rubin, Jeremy Stone, Aisha Haley Bianchi, Benjamin J. Capodanno, Estelle Y. Da, Mafalda Dias, Daniel Esposito, Jonathan Frazer, Yunfan Fu, Sally B. Grindstaff, Matthew R. Harrington, Iris Li, Abbye E. McEwen, Joseph K. Min, Nick Moore, Olivia G. Moscatelli, Jesslyn Ong, Polina V. Polunina, Joshua E. Rollins, Nathan J. Rollins, Ashley E. Snyder, Amy Tam, Matthew J. Wakefield, Shenyi Sunny Ye, Lea M. Starita, Vanessa L. Bryant, Debora S. Marks, Douglas M. Fowler

“…Here we describe the 2024 update to MaveDB ( https://www.mavedb.org/ ) with four key improvements to the MAVE community’s database of record: more available data including over 7 million variant effect measurements, an improved data model supporting assays such as saturation genome editing, new built-in exploration and visualization tools, and powerful APIs for data federation and streamlined submission and access. …”
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Functional genomics implicates ebony in the black pupae phenotype of tephritid fruit flies by Daniel F. Paulo, Thu N. M. Nguyen, Chris M. Ward, Renee L. Corpuz, Angela N. Kauwe, Pedro Rendon, Rocio E. Y. Ruano, Amanda A. S. Cardoso, Georgia Gouvi, Elisabeth Fung, Peter Crisp, Anzu Okada, Amanda Choo, Christian Stauffer, Kostas Bourtzis, Sheina B. Sim, Simon W. Baxter, Scott M. Geib

“…In tephritids, understanding the molecular basis of mutant traits is also crucial for applied entomology, enabling the creation of genetic sexing strains through genome editing, thus facilitating sex-sorting before sterile insect releases. …”
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