Interspecific hybridization and cell engineering of lettuce (<i>Lactuca</i> L.) by N. A. Zagnukhina, A. B. Kurina

“…This technique faces problems associated with complicated protoplast regeneration and the loss of reproductive ability in hybrids. Genome-editing methods are more effective and better controllable, but society is still wary of any interference with the plant genome and legally regulates the sale of GM products as food. …”
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Modern and future forestry based on biotechnology by Shihui Niu, Jihua Ding, Changzheng Xu, Jing Wang

“…In the last 2 decades, advances in biotechnologies such as high‐throughput sequencing and genome editing are transforming tree breeding and forestry. …”
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Role of Verticillium dahliae effectors in interaction with cotton plants by Lingling Yang, Tingyuan Fu, Ruichen Sha, Guihuan Wei, Yuhe Shen, Zhen Jiao, Bing Li

“…Furthermore, it highlighted the potential of effectors for engineering resistance cotton plants against Verticillium wilt, aiming to provide a reference for the creation of cotton disease-resistant germplasm resources by host genome editing and other methods.…”
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The autophagy component LC3 regulates lymphocyte adhesion via LFA1 transport in response to outside-in signaling by Naoyuki Kondo, Yuko Mimori-Kiyosue, Keizo Tokuhiro, Giuseppe Pezzotti, Tatsuo Kinashi

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A chromosome-level genome assembly of wild silkmoth, Bombyx mandarina by Jung Lee, Takashi Kiuchi, Katsushi Yamaguchi, Shuji Shigenobu, Atsushi Toyoda, Toru Shimada

“…ATAC-seq data comprehensively detected open chromosome regions, which will benefit when CRISPR/Cas9-mediated genome editing is conducted.…”
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Creation of a novel zebrafish model with low DHA status to study the role of maternal nutrition during neurodevelopment by Katherine M. Ranard, Bruce Appel

“…Here, we create a novel DHA-deficient zebrafish model by 1) disrupting elovl2, a key gene in the DHA biosynthesis pathway, via CRISPR/Cas9 genome editing, and 2) feeding mothers a DHA-deficient diet. …”
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Breeding for disease resilience: opportunities to manage polymicrobial challenge and improve commercial performance in the pig industry by Xuechun Bai, Graham S. Plastow

“…The next steps for managing polymicrobial challenges faced by the pig industry will include the development of large-scale multi-omics data, new phenotyping technologies, and mathematical and statistical methods adapted to these data. Genome editing to produce pigs resistant to major diseases may complement selection for disease resilience along with continued efforts in the more traditional areas of biosecurity, vaccination and treatment. …”
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Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing by Sol A Jeon, Jong Lyul Park, Jong-Hwan Kim, Jeong Hwan Kim, Yong Sung Kim, Jin Cheon Kim, Seon-Young Kim

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The Potential Use of the CRISPR-Cas System for HIV-1 Gene Therapy by Gabriela De Nardi Sanches-da-Silva, Luiza Fonseca Sales Medeiros, Fabio Mitsuo Lima

“…The CRISPR- (clustered regularly interspaced short palindromic repeat-) Cas system is a genome-editing method which uses a guide RNA, a complementary sequence to the interested site, recruiting a nuclease that can break the viral or the host cell genetic material. …”
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Prevalent integration of genomic repetitive and regulatory elements and donor sequences at CRISPR-Cas9-induced breaks by Chongwei Bi, Baolei Yuan, Yingzi Zhang, Mengge Wang, Yeteng Tian, Mo Li

“…Abstract CRISPR-Cas9 genome editing has been extensively applied in both academia and clinical settings, but its genotoxic risks, including large insertions (LgIns), remain poorly studied due to methodological limitations. …”
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Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo by Yilai Shu, Yong Tao, Wenyan Li, Jun Shen, Zhengmin Wang, Zheng-Yi Chen

“…To identify adenovirus vectors that target specific cell subtypes in the inner ear, we studied three adenovirus vectors, carrying a reporter gene encoding green fluorescent protein (GFP) from two vendors or with a genome editing gene Cre recombinase (Cre), by injection into postnatal days 0 (P0) and 4 (P4) mouse cochlea through scala media by cochleostomy in vivo. …”
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Breeding for postharvest physiological deterioration in cassava: problems and strategies by Wilton Mbinda, Asunta Mukami

“…Continuous improvements based on the new plant breeding technologies (genome editing, speeding breeding and RNA-dependent DNA methylation) in cassava and innovations in postharvest handling and storage of the storage roots are expected to provide sustainable solutions for PPD constraints and make cassava an important food security and nutrition and industrial crop.…”
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Mismatch prime editing gRNA increased efficiency and reduced indels by Jidong Fei, Dongdong Zhao, Caiyi Pang, Ju Li, Siwei Li, Wentao Qiao, Juan Tan, Changhao Bi, Xueli Zhang

“…Abstract Prime editing enables precise and efficient genome editing, but its efficacy is hindered by pegRNA’s 3’ extension, forming secondary structures due to high complementarity with the protospacer. …”
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CRISPR-Cas9 in basic and translational aspects of cancer therapy by Maryam Samareh Salavatipour, Zahra Poursalehi, Negin Hosseini Rouzbahani, Sohaib Mohammadyar, Mohammad Vasei

“…Methods: By review of the existing literature on application of CRISPR-Cas9 in cancer, different databases, such as PubMed and Google Scholar, we started data collection for "CRISPR-Cas9", "Genome Editing", "Cancer", "Solid tumors", "Hematologic malignancy" "Immunotherapy", "Diagnosis", "Drug resistance" phrases. …”
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Improvements in pig agriculture through gene editing by Kristin M. Whitworth, Jonathan A. Green, Bethany K. Redel, Rodney D. Geisert, Kiho Lee, Bhanu P. Telugu, Kevin D. Wells, Randall S. Prather

“…The availability of genome editing tools that allow for the introduction of precise genetic modification at a single nucleotide resolution, while also facilitating large transgene integration in the target population, offers a solution. …”
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Bone‐Targeted Fluoropeptide Nanoparticle Inhibits NF‐κB Signaling to Treat Osteosarcoma and Tumor‐Induced Bone Destruction by Lin Li, Guangyu Rong, Xin Gao, Yiyun Cheng, Zhengwang Sun, Xiaopan Cai, Jianru Xiao

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Removing limitations surrounding terpenoid biosynthesis by biotechnological techniques in Ferula sp.: A review by Zahra Aghaali, Jun-Li Yang, Mohammad Reza Naghavi, Meisam Zargar

“…This review examines the potential of hairy root culture, CRISPR/Cas-mediated genome editing, and metabolic engineering, including gene overexpression and enzyme engineering, for enhancing Ferula capacity tailored to industrial and medicinal needs. …”
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<i>Macrostomum lignano</i> as a model to study the genetics and genomics of parasitic flatworms by K. V. Ustyantsev, V. Yu. Vavilova, A. G. Blinov, E. V. Berezikov

“…All this makes M. lignano amenable to the most modern approaches of forward and reverse genetics, such as transposon insertional mutagenesis and methods of targeted genome editing by the CRISPR/Cas9 system. Due to the availability of an increasing number of genome and transcriptome assemblies of different parasitic flatworm species, new knowledge generated by studying M. lignano can be easily translated to parasitic f latworms with the help of modern bioinformatic methods of comparative genomics and transcriptomics. …”
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High-throughput tracking enables systematic phenotyping and drug repurposing in C. elegans disease models by Thomas J O'Brien, Ida L Barlow, Luigi Feriani, André EX Brown

“…Here, we show that high-throughput imaging and quantitative phenotyping can be conducted systematically on a panel of C. elegans disease model strains. We used CRISPR genome-editing to create 25 worm models of human Mendelian diseases and phenotyped them using a single standardised assay. …”
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Understanding Rett Syndrome: Genetic, clinical and therapeutic perspectives - literature review by Sylwia Urbańska, Aleksandra Witas, Martyna Borowska-Łygan, Justyna Pięta

“…Recent studies show positive effects of trofinetide treatment, and gene therapy, genome editing and ataluren promise to be a promising treatment for RTT. …”
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