Structural insights into how Cas9 targets nucleosomes by Reina Nagamura, Tomoya Kujirai, Junko Kato, Yutaro Shuto, Tsukasa Kusakizako, Hisato Hirano, Masaki Endo, Seiichi Toki, Hiroaki Saika, Hitoshi Kurumizaka, Osamu Nureki

“…Abstract The CRISPR-associated endonuclease Cas9 derived from prokaryotes is used as a genome editing, which targets specific genomic loci by single guide RNAs (sgRNAs). …”
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Bioinformatics services for analyzing massive genomic datasets by Gunhwan Ko, Pan-Gyu Kim, Youngbum Cho, Seongmun Jeong, Jae-Yoon Kim, Kyoung Hyoun Kim, Ho-Yeon Lee, Jiyeon Han, Namhee Yu, Seokjin Ham, Insoon Jang, Byunghee Kang, Sunguk Shin, Lian Kim, Seung-Won Lee, Dougu Nam, Jihyun F. Kim, Namshin Kim, Seon-Young Kim, Sanghyuk Lee, Tae-Young Roh, Byungwook Lee

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Multi-gene precision editing tool using CRISPR-Cas12a/Cpf1 system in Ogataea polymorpha by Senqin Hou, Shibin Yang, Wenqin Bai

“…However, the lack of precise and efficient genome editing tools severely hinders the construction of cell factories. …”
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Generation of Whole-Genome Sequencing Data for Comparing Primary and Castration-Resistant Prostate Cancer by Jong-Lyul Park, Seon-Kyu Kim, Jeong-Hwan Kim, Seok Joong Yun, Wun-Jae Kim, Won Tae Kim, Pildu Jeong, Ho Won Kang, Seon-Young Kim

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Manipulations with early mouse embryos for generation of genetically modified animals by A. N. Korablev, I. A. Serova, B. V. Skryabin

“…Recently, genome-editing technologies have  become more efficient and accessible. …”
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Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells by Dingbo Zhang, Fiona Parth, Laura Matos da Silva, Teng-Cheong Ha, Axel Schambach, Jens Boch

“…The engineered SsdA is a highly efficient genome editing tool.…”
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Recent Updates on Induced Pluripotent Stem Cells in Hematological Disorders by Methichit Wattanapanitch

“…Furthermore, recent advances in genome editing technology in combination with the iPSC technology have provided a versatile platform for studying stem cell biology and regenerative medicine. …”
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Mycobacteria Exploit Host GPR84 to Dampen Pro-Inflammatory Responses and Promote Infection in Macrophages by Reziya Wumaier, Ke Zhang, Jing Zhou, Zilu Wen, Zihan Chen, Geyang Luo, Hao Wang, Juliang Qin, Bing Du, Hua Ren, Yanzheng Song, Qian Gao, Bo Yan

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Rational engineering of minimally immunogenic nucleases for gene therapy by Rumya Raghavan, Mirco J. Friedrich, Indigo King, Samuel Chau-Duy-Tam Vo, Daniel Strebinger, Blake Lash, Michael Kilian, Michael Platten, Rhiannon K. Macrae, Yifan Song, Lucas Nivon, Feng Zhang

“…Abstract Genome editing using CRISPR-Cas systems is a promising avenue for the treatment of genetic diseases. …”
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Prospects for mineral biofortification of wheat: classical breeding and agronomy by I. N. Leonova, E. V. Ageeva, V. K. Shumny

“…Despite the promise of transgenesis and genome editing, these labor-intensive methods require significant investments, so these technologies, when applied to wheat, are still at the development stage and cannot be applied routinely. …”
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DNA targeting by compact Cas9d and its resurrected ancestor by Rodrigo Fregoso Ocampo, Jack P. K. Bravo, Tyler L. Dangerfield, Isabel Nocedal, Samatar A. Jirde, Lisa M. Alexander, Nicole C. Thomas, Anjali Das, Sarah Nielson, Kenneth A. Johnson, Christopher T. Brown, Cristina N. Butterfield, Daniela S. A. Goltsman, David W. Taylor

“…Abstract Type II CRISPR endonucleases are widely used programmable genome editing tools. Recently, CRISPR-Cas systems with highly compact nucleases have been discovered, including Cas9d (a type II-D nuclease). …”
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A Review: CRISPR Cas System and the Mechanism With an Inhibition of Binding of CRISPR Cas‐9 by Akram Hosen, Md Nakibul Hasan Nishat, MD. Mohaimin Hossain Soaib, Omar Sharif Sharkar, Md. Sahabuddin, Imdadul Haque Sharif, Sujay Kumar Bhajan

“…ABSTRACT CRISPR Cas system is a revolutionary and precise genome editing system that induces bacterial and archaeal adaptive immunity and already has shown several promising applications in the treatment of genetic diseases and detection of nucleic acid targets. …”
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Mesenchymal Stem Cells and Regenerative Medicine by Huseyin Sumer, Jun Liu, Sangho Roh

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Advances in lignocellulosic feedstocks for bioenergy and bioproducts by Daniel B. Sulis, Nathalie Lavoine, Heike Sederoff, Xiao Jiang, Barbara M. Marques, Kai Lan, Carlos Cofre-Vega, Rodolphe Barrangou, Jack P. Wang

“…It also explores emerging biotechnologies such as CRISPR-based genome editing informed by machine learning, aimed at improving feedstock traits and reducing the environmental impact of fossil fuel dependence.…”
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PD-L1 knockout or ZG16 overexpression inhibits PDAC progression and modulates TAM polarization by Hui Meng, Manman Nan, Yizhen Li, Yi Ding, Xiaokun Fang, Weiqian Ma, Mingzhi Zhang

“…CRISPR/Cas9-mediated genome editing has the potential to delete PD-L1 both on the cell surface and inside the cell, thereby inhibiting tumor growth and migration and overcoming immunosuppression. …”
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Engineering CRISPR for emerging therapeutic applications by Xiaoqi Sun, Zhuang Liu

“…The discovery of the ability of CRISPR–Cas9 to cut targeted DNA sequences at predetermined sites has introduced a new era of precision and flexibility in genome editing. Building on this classic CRISPR–Cas system, the discovery of other diverse CRISPR systems and their further engineering have produced various powerful tools for gene editing and modulation at the genomic, chromatin, and RNA levels. …”
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Canadian perspectives on food security and plant breeding by Claire Williams, Savannah Gleim, Stuart J. Smyth

“…Conclusions Canadians lack basic knowledge about modern plant breeding practices and technologies and possess high levels of uncertainty regarding the potential for benefits or externalities to develop from the commercialization of new genome editing plant breeding technologies.…”
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Dual α-globin-truncated erythropoietin receptor knockin restores hemoglobin production in α-thalassemia-derived erythroid cells by Simon N. Chu, Eric Soupene, Devesh Sharma, Roshani Sinha, Travis McCreary, Britney Hernandez, Huifeng Shen, Beeke Wienert, Chance Bowman, Han Yin, Benjamin J. Lesch, Kun Jia, Kathleen A. Romero, Zachary Kostamo, Yankai Zhang, Tammy Tran, Marco Cordero, Shota Homma, Jessica P. Hampton, James M. Gardner, Bruce R. Conklin, Tippi C. MacKenzie, Vivien A. Sheehan, Matthew H. Porteus, M. Kyle Cromer

“…Despite progress in genome editing treatments for β-thalassemia, there is no analogous curative option for α-thalassemia. …”
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Principles and medical applications of gene editing by CRISPR / Cas by Rolando Sánchez Artigas, María Teresa Díaz Armas, Raisa Rodríguez Duque, Pedro Enrique Miguel Soca

“…In order to describe the principles and medical application of this technology, a bibliographic review was carried out in Pubmed, SciELO, academic Google and the Cochrane Library, with the descriptors “gene editing”, “genome editing”, “CRISPR-Cas systems”, and “CRISPR-associated protein 9”. …”
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The role of pangenomics in orphan crop improvement by Haifei Hu, Junliang Zhao, William J. W. Thomas, Jacqueline Batley, David Edwards

“…Orphan crop research has now entered the pangenomics era and applying these pangenomes with advanced selection methods and genome editing technologies can transform these neglected species into crops of broader agricultural significance.…”
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