Engineering a bacterial toxin deaminase from the DYW-family into a novel cytosine base editor for plants and mammalian cells by Dingbo Zhang, Fiona Parth, Laura Matos da Silva, Teng-Cheong Ha, Axel Schambach, Jens Boch

“…The engineered SsdA is a highly efficient genome editing tool.…”
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Recent Updates on Induced Pluripotent Stem Cells in Hematological Disorders by Methichit Wattanapanitch

“…Furthermore, recent advances in genome editing technology in combination with the iPSC technology have provided a versatile platform for studying stem cell biology and regenerative medicine. …”
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Mycobacteria Exploit Host GPR84 to Dampen Pro-Inflammatory Responses and Promote Infection in Macrophages by Reziya Wumaier, Ke Zhang, Jing Zhou, Zilu Wen, Zihan Chen, Geyang Luo, Hao Wang, Juliang Qin, Bing Du, Hua Ren, Yanzheng Song, Qian Gao, Bo Yan

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Prospects for mineral biofortification of wheat: classical breeding and agronomy by I. N. Leonova, E. V. Ageeva, V. K. Shumny

“…Despite the promise of transgenesis and genome editing, these labor-intensive methods require significant investments, so these technologies, when applied to wheat, are still at the development stage and cannot be applied routinely. …”
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Mesenchymal Stem Cells and Regenerative Medicine by Huseyin Sumer, Jun Liu, Sangho Roh

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Advances in lignocellulosic feedstocks for bioenergy and bioproducts by Daniel B. Sulis, Nathalie Lavoine, Heike Sederoff, Xiao Jiang, Barbara M. Marques, Kai Lan, Carlos Cofre-Vega, Rodolphe Barrangou, Jack P. Wang

“…It also explores emerging biotechnologies such as CRISPR-based genome editing informed by machine learning, aimed at improving feedstock traits and reducing the environmental impact of fossil fuel dependence.…”
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PD-L1 knockout or ZG16 overexpression inhibits PDAC progression and modulates TAM polarization by Hui Meng, Manman Nan, Yizhen Li, Yi Ding, Xiaokun Fang, Weiqian Ma, Mingzhi Zhang

“…CRISPR/Cas9-mediated genome editing has the potential to delete PD-L1 both on the cell surface and inside the cell, thereby inhibiting tumor growth and migration and overcoming immunosuppression. …”
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Canadian perspectives on food security and plant breeding by Claire Williams, Savannah Gleim, Stuart J. Smyth

“…Conclusions Canadians lack basic knowledge about modern plant breeding practices and technologies and possess high levels of uncertainty regarding the potential for benefits or externalities to develop from the commercialization of new genome editing plant breeding technologies.…”
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Principles and medical applications of gene editing by CRISPR / Cas by Rolando Sánchez Artigas, María Teresa Díaz Armas, Raisa Rodríguez Duque, Pedro Enrique Miguel Soca

“…In order to describe the principles and medical application of this technology, a bibliographic review was carried out in Pubmed, SciELO, academic Google and the Cochrane Library, with the descriptors “gene editing”, “genome editing”, “CRISPR-Cas systems”, and “CRISPR-associated protein 9”. …”
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Interspecific hybridization and cell engineering of lettuce (<i>Lactuca</i> L.) by N. A. Zagnukhina, A. B. Kurina

“…This technique faces problems associated with complicated protoplast regeneration and the loss of reproductive ability in hybrids. Genome-editing methods are more effective and better controllable, but society is still wary of any interference with the plant genome and legally regulates the sale of GM products as food. …”
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Modern and future forestry based on biotechnology by Shihui Niu, Jihua Ding, Changzheng Xu, Jing Wang

“…In the last 2 decades, advances in biotechnologies such as high‐throughput sequencing and genome editing are transforming tree breeding and forestry. …”
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The autophagy component LC3 regulates lymphocyte adhesion via LFA1 transport in response to outside-in signaling by Naoyuki Kondo, Yuko Mimori-Kiyosue, Keizo Tokuhiro, Giuseppe Pezzotti, Tatsuo Kinashi

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Creation of a novel zebrafish model with low DHA status to study the role of maternal nutrition during neurodevelopment by Katherine M. Ranard, Bruce Appel

“…Here, we create a novel DHA-deficient zebrafish model by 1) disrupting elovl2, a key gene in the DHA biosynthesis pathway, via CRISPR/Cas9 genome editing, and 2) feeding mothers a DHA-deficient diet. …”
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Breeding for disease resilience: opportunities to manage polymicrobial challenge and improve commercial performance in the pig industry by Xuechun Bai, Graham S. Plastow

“…The next steps for managing polymicrobial challenges faced by the pig industry will include the development of large-scale multi-omics data, new phenotyping technologies, and mathematical and statistical methods adapted to these data. Genome editing to produce pigs resistant to major diseases may complement selection for disease resilience along with continued efforts in the more traditional areas of biosecurity, vaccination and treatment. …”
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Comparison of the MGISEQ-2000 and Illumina HiSeq 4000 sequencing platforms for RNA sequencing by Sol A Jeon, Jong Lyul Park, Jong-Hwan Kim, Jeong Hwan Kim, Yong Sung Kim, Jin Cheon Kim, Seon-Young Kim

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The Potential Use of the CRISPR-Cas System for HIV-1 Gene Therapy by Gabriela De Nardi Sanches-da-Silva, Luiza Fonseca Sales Medeiros, Fabio Mitsuo Lima

“…The CRISPR- (clustered regularly interspaced short palindromic repeat-) Cas system is a genome-editing method which uses a guide RNA, a complementary sequence to the interested site, recruiting a nuclease that can break the viral or the host cell genetic material. …”
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Prevalent integration of genomic repetitive and regulatory elements and donor sequences at CRISPR-Cas9-induced breaks by Chongwei Bi, Baolei Yuan, Yingzi Zhang, Mengge Wang, Yeteng Tian, Mo Li

“…Abstract CRISPR-Cas9 genome editing has been extensively applied in both academia and clinical settings, but its genotoxic risks, including large insertions (LgIns), remain poorly studied due to methodological limitations. …”
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Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo by Yilai Shu, Yong Tao, Wenyan Li, Jun Shen, Zhengmin Wang, Zheng-Yi Chen

“…To identify adenovirus vectors that target specific cell subtypes in the inner ear, we studied three adenovirus vectors, carrying a reporter gene encoding green fluorescent protein (GFP) from two vendors or with a genome editing gene Cre recombinase (Cre), by injection into postnatal days 0 (P0) and 4 (P4) mouse cochlea through scala media by cochleostomy in vivo. …”
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Breeding for postharvest physiological deterioration in cassava: problems and strategies by Wilton Mbinda, Asunta Mukami

“…Continuous improvements based on the new plant breeding technologies (genome editing, speeding breeding and RNA-dependent DNA methylation) in cassava and innovations in postharvest handling and storage of the storage roots are expected to provide sustainable solutions for PPD constraints and make cassava an important food security and nutrition and industrial crop.…”
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CRISPR-Cas9 in basic and translational aspects of cancer therapy by Maryam Samareh Salavatipour, Zahra Poursalehi, Negin Hosseini Rouzbahani, Sohaib Mohammadyar, Mohammad Vasei

“…Methods: By review of the existing literature on application of CRISPR-Cas9 in cancer, different databases, such as PubMed and Google Scholar, we started data collection for "CRISPR-Cas9", "Genome Editing", "Cancer", "Solid tumors", "Hematologic malignancy" "Immunotherapy", "Diagnosis", "Drug resistance" phrases. …”
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